• Maxillofacial Plastic and Reconstructive Surgery
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• v.40
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• pp.10.1-10.6
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• 2018

Background: The objective of this study was to evaluate the changes in gene expression after incubation of cells with proteins released from different silk mat layers. Methods: A silk cocoon from Bombyx mori was separated into four layers of equal thickness. The layers were numbered from 1 to 4 (from the inner to the outer layer). The proteins were released by sonication of a silk mat layer in normal saline. The concentration of proteins was determined by spectrophotometry. They were incubated with RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Results: Layer 1 and 4 groups had higher protein concentrations compared to those in layer 2 and 3 groups. The genes associated with inflammation and angiogenesis showed significantly higher expression in layer 1 and 4 groups. The results of qRT-PCR were in agreement with those of the cDNA microarray analysis. Conclusions: The silk mat from the middle portion of the silkworm cocoon yielded a lower protein release and caused an insignificant change in the expression of genes that are associated with inflammation and angiogenesis.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 1997.06a
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• pp.1-21
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• 1997

Silk is praised "Queen of Fiber", which wrote a glorious page in the history of human adornment culture. Thanks to the development of science and technology, silk production has been increased dramatically during last half century. Nowadays, as "Healthy Fiber" silk lets all the globe to be forming the "Silk Boom" Like in other scientific fields, a great deal of progress has been made in sericultural science and technology, which also accelerated the development of multipurpose utilization of sercultural recourses. The main tendency of the sericulture development is raising the quality of cocoon, and at the same time, increasing the output per unit mulberry area and labor production efficiency. The chief measure of raising the cocoon quality is breeding and selecting the new mulberry and silkworm varieties, preventing and curing the diseases and pests and improving the environment of mounting, and the symble for increasing the labor productivity is carrying out the technological system of saving labor and operation ease, and enlarging the production scale. As a natural protein fiber, silk will be still a favorite with human in the next century, because its structure and properties can not be replaced by other any fiber. The 21st century is the biological time, and measures of developing sericulture will use the achievement on the field of molecular biology and biotechnology to carry out the molecular breeding, development regulating, silkworm rearing industrializing, exploiting the new silk and its new usage, utilizing the sericultural resources, and producing the special protein from silkworm as a host of expressing exogenous genes so that make the sericulture to contribute more what it can do for the human being.

• Proceedings of the Zoological Society Korea Conference
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• 2000.04a
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• pp.69.2-69
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• 2000

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.135.1-135
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• 1996

No Abstract, See Full Text

• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.2
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• pp.87-90
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• 2000

This paper has reviewed the China Sericulture history in the 20th century. On the basis of the current sericulture production, sericulture area distribution, problems and challenges, it has been forecasted that China sericulture will be developed as following points: 1) On a stable basis, cocoon production increased gradually to a suitable amount; 2) Quality cocoon production bases established to form a regionalized, industrialized and specialized sericulture structure; 3) With increase of householding rearing capacities, rearing facilities improved; 4) Production efficiency raised and silk product quality improved through technical innovation; 5) New and diversified products developed from Sericultural resources with new and high technologies.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.1
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• pp.13-19
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• 2005

Biotechnological researches in Central Asian Research Institute of Sericulture were developed since 1981 when laboratory of artificial diets for mulberry silk­worm was created. The researches were directed not only to creation of accessible artificial diets (AD) for off-season mass rearing of economical beneficial insect and for industrialization of sericulture but also to obtaining of easy reproducible source of animal proteins, carbohydrates, lipids, enzymes, enzymes inhibitors and other metabolites - bioresource for manufacture of oriental medicine preparations. Space experiments with silkworms became possible with use of AD. These experiments used mulberry silkworm as high effective board test organism with outstanding developmental, reproductive and transgenic properties having impor­tant significance in future of cosmonautics. Introduction to practice of bioprotective and ecologically pure method of drying and sterilizing of green cocoons and by-products of silk manufacture allows to increase efficiency of cocoons reeling technology as well as to preserve nativity of biological active substances in mulberry silkworm pupae that is unachievable by traditional methods of raw material processing. New spheres of application of two important silk proteins - fibroin and sericin in medicine, cosmetics, for bio- and nanotechnology are opening with use of biotechnological approaches. At present the achievements and experience of R & D in these fields are used in creation of modern pests biocontrol agents. Schemes of efficient processing of cocoon raw material, by-products and wastes of sericulture and cocoon processing are devel- Institute of Physiology and Biophysics, Uzbek Academy of Sciences, I, Niyazov street, 700095, Tashkent, Uzbekistan. oped for obtaining of traditional and new consumer products.

• Journal of Sericultural and Entomological Science
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• v.49 no.1
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• pp.14-17
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• 2007

A new silkworm variety Golden silk for spring rearing season is $F_1$ hybrid between Jam311, a Japanese race bred from introduction breeding and Jam312, a Chinese race from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2006, "Golden silk" was showed 7 percent higher in hatchability than authorized silk-worm races laval. "Golden silk" was receded higher 1.7 percent, 2.2 percent in pupation rate, best cocoon rate respectively than Chunsujam. The larval period shortened for 1.12 day even though low record to the several items including productivity than Chunsujam. "Golden silk" was showed 1,031 m, 2.97 denier in length of cocoon filament, weight of cocoon filament respectively. The concentration of Deoxynojirimycin(DNJ) was measured 4.34 mg in "Golden silk", high then to "Chunsujam"(3.81 mg). In the Paecilomyces tenuipes production ability test, the pupal weight of "Golden silk" was 1.20g.

• Journal of Sericultural and Entomological Science
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• v.15 no.1
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• pp.27-30
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• 1973

The present research has been carried out in an attempt to obtain fundamental data which contribute to enhansing the accuracy of cocoon reeling test and improving the method of it more effectively. The results obtained are as follows: 1. The standard deviation and coefficient of variation of percentage of reelability and of percentage of raw silk yield to the sample size tended to show a gradual decrease according to the increasing of the sample size. However, the significant differences in the standard deviation and coefficient of variation of percentage of reelability and of percentage of raw silk yield between the different sizes of the samples having more than 300 cocoons have not been observed according to their sizes. 2. The significant difference in the standard errors from percentage of reelability between the samples of 300 cocoons and 400 cocoons have not been observed. 3. The significant difference in the standad errors from percentage of raw silk yield between the samples of 300 cocoons, 500 cocoons and 600 cocoons have not been observed. 4. The significant difference in the standard deviation, the standard errors and coefficient of variation to the length of a bave between the different sizes of the samples have not been shown. 5. From the above result, it is estimated that the optimum sire of the sample for the cocoon reeling test is the 300 cocoons.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.83-88
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• 2017

Silk is appealing materials for many biomedical applications involving tissue engineering and implantable devices, because of its biocompatibility, environmental stability, controlled proteolytic biodegradability and morphologic flexibility. Silk matrix is required for the treatment of a wide wound area, but the present silk matrix is made by the second processing, and thus, the labor and the cost are high. In this work, we investigated the optimal production condition of natural silk 3D matrix using the silkworms and invented Automatic Silk Matrix Making Machine (ASMMM) for natural silk 3D matrix production. As a result, we determined that optimal production condition for making A4 paper size natural silk 3D matrix was used Rough aquarelle paper on surface at $25^{\circ}C$ and 30 silkworm larvae. These results are expected to provide basic data for the efficient production of the natural silk 3D matrix, and it is suggested that the produced natural silk 3D matrix is useful as a medical biomaterials.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.92-96
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• 2015

It is known that silk protein supports effectively proliferation of cell such as insect cell and hybridoma cell. Although there are many varieties of Bombyx mori silkworm, the effect of silkworm varieties on cell proliferation has not been considered in detail. We studied that characteristics of silk cocoon obtained from Baegokjam, Kumokjam, Daeseongjam silkworm varieties and whether silk protein affected cell proliferation or not. Silk sericin was prepared under high temperature and high pressure condition. Silk fibroin was prepared using $CaCl_2:H_2O:EtOH$ with different dissolution time. As a result, there are differences in silk cocoon from different silkworm varieties about cell proliferation. The proliferation was accelerated in the presence of Baegokjam silk sericin and Kumokjam silk fibroin with 5hr dissolution time. We expect that silk proteins could be a preferable culture medium supplement for stimulating the proliferation of cell. Then, this results suggest silk as a new material for medium supplement replacing with fetal bovine serum.