• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.143-146
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• 2004

A surface plasmon resonance (SPR) imaging system was constructed and used to detect the hexahistidine-ubiquitin-tagged human parathyroid hormone fragment (His$\sub$6/-Ub-hPTHF(1-34)) expressed in Escherichia coli. The hexahistidine-specific antibody was immobilized on a thin gold film coated with ProLinker$\^$TM/ B, a novel calixcrown derivative with a bifunctional coupling property that permits efficient immobilizaton of capture proteins on solid matrices. The soluble and insoluble fractions of an E. coli cell lysate were spotted onto the antibody-coated gold chip, which was then washed with buffer (pH 7.4) solution and dried. SPR imaging measurements were carried out to detect the expressed His$\sub$6/-Ub-hPTHF(1-34). There was no discernible protein image in the uninduced cell lysate, indicating that non-specific binding of contaminant proteins did not occur on the gold chip surface. It is expected that the approach used here to detect affinity-tagged recombinant proteins using an SPR imaging technique could be used as a powerful tool for the analyses of a number of proteins in a high-throughput mode.

• 비파괴검사학회지
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• 제26권1호
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• pp.7-11
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• 2006

기계부품의 세라믹 코팅 층은 사용 중 그 표면특성이 변하므로 잔류수명 예측과 신뢰성 확보를 위해 비파괴적으로 세라믹 코팅 층의 특성을 평가하는 것은 매우 중요하다. 이를 위해 본 인구는 AISI 1045 강과 오스테나이트 스테인리스 304 강 모재위에 매우 얇은 TiN 세라믹 층을 코팅한 시편에 내하여 누설 레일리(Rayleigh) 표면파를 이용한 TiN 코팅부재의 특성평가를 시도하였다. 후방복사 측정기법으로 코팅 층 내의 표면파의 전파특성이 평가되었고 표면 거칠기, 코팅 층 두께, 마모조긴 둥이 다양한 시편들에 대해 후방복사 프로파일이 측정되었다. 후방복사 프로파일의 정점각과 크기는 시편의 조건들에 따라 민감하게 변하였고, 정점각의 증가는 코팅 층 접합 강도의 저하로 설명되었다. 결과들은 후방복사 기법을 이용한 표면파의 분산성 평가가 코팅 층의 특성 평가에 매우 유용함을 보여주었다.

• 방사선산업학회지
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• 제5권4호
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• pp.371-376
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• 2011

The surface property of the materials used in tissue engineering application has been essential to regulate cellular behaviors by directing their adhesion on the materials. To modulate surface property of the synthetic biodegradable materials, a variety of surface modification techniques have used to introduced surface functional groups or bioactive molecules, recently polydopamine coating method have been introduce as a facile modification method which can be coated on various materials such as polymers, metals, and ceramics regardless of their surface property. However, there are no reports about the degree of polydopamine coating on the materials with different hydrophilicity. In the present study, we prepared acrylic acid grafted nanofibrous meshes using electron-beam irradiation, and then coated meshes with polydopamine. Polydopamine successfully coated on the all meshes, both properties of acrylic acid and polydopamine were detected on the meshes. In addition, the degree of polydopamine deposition on the materials has been altered according to surface hydrophilicity, which was approximately 8-times greater than those on the non-modified materials. In conclusion, dual effect from the acrylic acid grafting and polydopamine may give a chance as a alternative tool in tissue engineering application.

• 한국산학기술학회논문지
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• 제13권2호
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• pp.471-477
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• 2012

SCM420재질의 기어를 담금질을 실시한 후 초경호브(Hob)에 PACVD 코팅처리 후 표면경도가 높은 표면(HRC 60)을 절삭하였다. 코팅처리 없이 난삭재(難削材)로 분류되는 경도가 큰것, 경한 물질을 포함한 것, 강도가 높은 것에 공구수명과 생산성을 향상에는 한계성을 가지고 있다. 이를 개선하기 위해 초경호브에 TiCN코팅처리 전후에 대하여 Skiving 절삭으로 코팅처리 한 호브가 가공성이 좋고, 공구마멸이 적어, 공구수명이 2.5배 증가하는 결과를 얻었다. 실험은 CNC 스카이빙 호빙머신을 이용하여 습식절삭으로 절삭속도와 이송량으로 다양한 조건을 적용하여 공구마멸과 표면거칠기 데이터를 얻었다. 실험결과 조건 2에서(V=200m/min F=0.7mm/rev) Cutting speed가 절삭표면에 Feed Mark가 미세하고, 표면거칠기는 Rmax $4.7{\mu}m$(Ra $1.19{\mu}m$)의 데이터를 얻었다.

• 한국기계가공학회지
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• 제6권4호
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• pp.22-27
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• 2007

This study aims to find the optimal cutting conditions, when ellipse mirrors consisted Aluminum alloy were made it the Millimeter-Wave Interferometer System mirror with several tools on the High-Speed Machine. Machining technique for precision machining characteristics of ellipse mirrors consisted Al6061 matter by Ball endmill is reported in this paper., Results of machining on the High-Speed Machine(using NCD(Natural Crystalline diamond), WC and coated TiAlN ${\phi}6mm$ ball endmill tool) had measurement of surface roughness and form accuracy with cutting conditions(the Feed rate, the Depth of cut and the Cutting speed). the Millimeter-Wave Interferometer System ellipse mirror had been machined foundational precision machining characteristics of aluminum.

• Steel and Composite Structures
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• 제23권2호
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• pp.153-160
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• 2017

It is extremely important to be aware of the ballistic performances of engineering materials in order to be able to choose the lightest armor providing full ballistic protection in civil and military applications. Therefore, ballistic tests are an important part of armor design process. In this study, ballistic performance of plates made of carbon steel and cold worked tool steel against 7.62 mm AP (armor-piercing) bullets was examined experimentally and numerically in accordance with NIJ standards. Samples in different sizes were prepared to demonstrate the effect of target thickness on ballistic performance. Some of these samples were coated with titanium nitride using physical vapor deposition (PVD) method. After examining all successful and unsuccessful samples at macro and micro levels, factors affecting ballistic performance were determined. Explicit non-linear analyses were made using Ls-Dyna software in order to confirm physical ballistic test results. It was observed that the ballistic features of steel plates used in simulations comply with actual physical test results.

• 한국생산제조학회지
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• 제20권2호
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• pp.162-168
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• 2011

In this research, nitrogen plasma source ion implantation(PSII) of non-coated tungsten carbide endmill tools was conducted with low heat treatment for increasing wear resistance. After the low heat treatment of PSIIed tools to give a homogeneity of wear resistance, the surface modification of tools was analyzed by hardness test, surface roughness and cutting forces. As for the resultant cutting forces, low heat treatment in temperature of $400^{\circ}C$ and $500^{\circ}C$ is stable because of low cutting resistance. The 20-minutes heat treated tool at spindle speed 25000rpm has superiority of surface roughness, Ra of $0.420{\mu}m$ and was found to have good wear resistance. The higher hardness value was obtained by increasing temperature from $300^{\circ}C$ to $600^{\circ}C$ for PSIIed tools with low heat treatment. As the PSIIed tools under 10minutes at temperature of $600^{\circ}C$ have the highest hardness as Hv of 2349.8, It was analyzed that temperature processing give much influences on hardness.

• Bulletin of the Korean Chemical Society
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• 제35권2호
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• pp.383-391
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• 2014

Biotin-S-S-Phosphine was designed and synthesized as a potential tool for a proteomic study of O-GlcNAcmodified proteins. This reagent features a disulfide linker between a triarylphosphine moiety, which allows selective conjugation to azide-containing proteins, and a biotin moiety that can allow easy isolation through its strong affinity toward avidin-coated solid beads. The disulfide linkage within this reagent can allow the easy release of the bound molecules of interest, which is difficult to achieve when a biotin:avidin pair is used alone, by reducing the disulfide bond of the reagent with DTT. Preliminary in vitro biological assays with azidelabeled and unlabeled cell lysates and a pure protein Nup62 showed that the Biotin-S-S-Phosphine reagent is highly reactive toward the free thiol groups of proteins. When a molecular tool with a disulfide linker is applied to the enrichment of the molecules of interest from other species, it is important to block the free-thiols of the sample using exhaustive alkylation prior to the Staudinger ligation reactions to restore the bioorthogonal nature of this reaction.

• 음성과학
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• 제10권4호
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• pp.181-187
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• 2003

This paper introduces a new zinc coated copper wire electrode with coiled cone shape which has low surface resistance and tolerance to the motion artifact for promontory stimulation electrically auditory brainstem responses (PSEABR). Auditory brainstem responses (ABR) can be used to predict hearing threshold level with a great deal of accuracy particularly for a young child who cannot cooperate mechanically and some hearing impaired who are exaggerating a hearing loss for economic compensation. While severe profound sensorineural hearing losses may not be implemented by auditory potentials, PSEABR is proven as a useful tool even for some sensorineural related hearing impaired. It was shown that PSEABR gives the electrical stimuli to promontory of the cochlear instead of giving acoustic stimuli. For this reason, PSEABR can be used as an alternative for cochlear implantation, and can also be used as an optimal device selection and neural information for MAP. It was found that the role of electrode is very important in PSEABR. Even though this cone-shaped electrode was applied in animal experiments, waveforms are well produced by PSEABR. Thus, it was concluded that cone-shaped electrode turned out to be a useful preoperative audiological evaluation tool in deciding time for cochlear implantation surgery.

• 한국공작기계학회논문집
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• 제14권2호
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• pp.1-7
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• 2005

In order to determine the wear Properties of PECVD ceramic coatings, wear process was evaluated using the coated pin of Falex Tribosystem. Coating materials deposited wear the TiC, TiN and Ti(CN). An experimental process was established to determine the tribological characteristics of friction and wear behavior under the variation of applied load, temperature and sliding distance by the Falex test machine. The experimental results indicate that TiN coating compared with TiC coating on e materials have e excellent friction and wear characteristics. However TiC coating compared i친 TiN coatings have a low friction coefficient with steel and good thermal stability, and Ti(CN) has the excellent anti-wear properly as well as the superiority of extreme pressure property. Compound coating compared wi simple coatings show improved tribological characteristics.