• 원예과학기술지
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• 제29권2호
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• pp.123-129
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• 2011

To identify unintended vertical gene-transfer rates from the developed transgenic plants, rapid and unequivocal techniques are needed to identify event-specific markers based on flanking sequences around the transgene and to distinguish zygosity such as homo- and hetero-zygosity. To facilitate evaluation of zygosity, a polymerase chain reaction technique was used to analyze a transgenic pepper line B20 (homozygote), P915 wild type (null zygote), and their F1 hybrids, which were used as transgene contaminated plants. First, we sequenced the 3'-flanking region of the T-DNA (1,277 bp) in the transgenic pepper event B20. Based on sequence information for the 3'- and 5'-flanking region of T-DNA provided in a previous study, a primer pair was designed to amplify full length T-DNA in B20. We successfully amplified the full length T-DNA containing 986 bp from the flanking regions of B20. In addition, a 1,040 bp PCR product, which was where the T-DNA was inserted, was amplified from P915. Finally, both full length T-DNA and the 1,040 bp fragment were simultaneously amplified in the F1 hybrids; P915 ${\times}$ B20, Pungchon ${\times}$ B20, Gumtap ${\times}$ B20. In the present study, we were able to identify zygosity among homozygous transgenic event B20, its wild type P915, and hemizygous F1 hybrids. Therefore, this novel zygosity identification technique, which is based on PCR, can be effectively used to examine gene flow for transgenic pepper event B20.

• KSBB Journal
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• 제24권3호
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• pp.279-286
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• 2009

방선균은 다양한 생리활성 물질을 이차대사산물로 생산하는 산업적으로 매우 유용한 미생물이다. 이에 따라 많은 연구진들이 방선균에 대한 분자생물학적 연구와 산업적 이용에 대한 연구들을 수행하고 있다. 방선균 중에서도 S. avermitilis는 강력한 구충효과가 있는 avermectin을 생산하지만, 또한 포유동물 세포의 미토콘드리아에서 산화적 인산화반응을 억제하는 oligomycin도 함께 생성된다. 따라서 S. avermitilis에서 oligomycin의 생성을 제거시키기 위하여 oligomycin synthetase gene을 disruption 시키기 위한 연구를 수행하였다. 이를 위하여 S. avermitilis로부터 cloning 한 oligomycin synthetase gene (olmA5)의 중앙부분에 apramycin resistance gene을 끼워 넣어 integration vector로 구축한 후에 S. avermitilis의 chromosomal DNA와의 homologous recombination에 의하여 olmA5 gene의 disruption을 유도하였다. Disruption mutants (olmA5::apra)는 PCR을 통해 olmA5 gene의 위치에 apramycin resistance gene이 존재하는 것으로 확인하였고, 또한 HPLC 분석을 통해 oligomycin 생합성이 완전히 제거된 것임을 확인하였다. 그러나 disruption mutant (olmA5::apra)를 이용하여 avermectin 만을 생산할 수 있었으나, avermectin의 생산량에는 거의 변화가 없었다. 이러한 mutants는 산업적으로 avermectin을 생산하기 위한 균주 개량의 훌륭한 source가 될 수 있을 것이다.

• 대한치과보철학회지
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• 제44권4호
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• pp.414-420
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• 2006

Purpose : The purpose of this study is to use finite element analysis to predict the fatigue life of an implant system subjected to fatigue load by mastication (chewing force). The reliability and the stability of implant system can be defined in terms of the fatigue strength. Not only an implant is expensive but also it is almost impossible to correct after it is inserted. From a bio-engineering standpoint, the fatigue strength of the dental implant system must be evaluated by simulation (FEA). Material and Methods Finite element analysis and fatigue test are performed to estimate the fatigue strength of the implant system. Mesh of implant is generated with the actual shape and size. In this paper, the fatigue strength of implant system is estimated. U-fit (T. Strong, Korea, internal type). The stress field in implant is calculated by elastic-plastic finite element analysis. The equivalent fatigue stress, considering the contact and preload stretching of a screw by torque for tightening an abutment, is obtained by means of Sine's method. To evaluate the reliability of the calculated fatigue strength, fatigue test is performed. Results: A comparison of the calculated fatigue strength with experimental data showed the validity and accuracy of the proposed method. The initiation points of the fatigue failure in the implant system exist in the region of high equivalent fatigue stress values. Conclusion: The above proposed method for fatigue life estimation tan be applied to other configurations of the differently designed and improved implant. In order to prove reliability of prototype implant, fatigue test should be executed. The proposed method is economical for the prediction of fatigue life because fatigue testing, which is time consuming and precision-dependent, is not required.

• The Korean Journal of Ceramics
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• 제6권3호
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• pp.276-279
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• 2000

Ferroelectric properties of $SrBi_2TaNbO_9$ (SBTN) thin films were changed by the amount of Bi content in SBTN. We suggested that the addition of excess Bi into the films could be accomplished by heat-treating $SBTN/Bi_2O_3/SBTN$ heterostructure fabricated by r.f. magnetron sputtering method. Excess Bi composition was controlled by the thickness of the sandwiched $Bi_2O_3$ from 0 to $400\;\AA$. When the SBTN thin films were inserted by $400\;{\AA}\;Bi_2O_3$ layer, $Bi_2Pt$ phase was formed as a second phase in SBTN films, resulting in poor ferroelectric properties. The onset temperature for hysteresis loop can be reduced by heat treating $SBTN/Bi_2O_3/SBTN$ heterostructure. The films with $SBTN/Bi_2O_3(100\;{\AA})/SBTN$ hetero-structure followed by annealing at $650^{\circ}C$ for 30 min show 2Pr and Ec of $5.66\;{\mu}C/\textrm{cm}^2$ and 54 kV/cm, respectively.

• 동굴
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• 제76호
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• pp.37-42
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• 2006

The Dielectric Barrier Discharge (DBD) is a nonequilibrium gas discharge that is generated in the space between two electrodes, which are separated by an insulating dielectric layer. The dielectric layer can be put on either of the two electrodes or be inserted in the space between two electrodes. If an AC or pulse high voltage is applied to the electrodes that is operated at applied frequency from 50Hz to several MHz and applied voltages from a few to a few tens of kilovolts rms, the breakdown can occur in working gas, resulting in large numbers of micro-discharges across the gap, the gas discharge is the so called DBD. Compared with most other means for nonequilibrium discharges, the main advantage of the DBD is that active species for chemical reaction can be produced at low temperature and atmospheric pressure without the vacuum set up, it also presents many unique physical and chemical process including light, heat, sound and electricity. This has led to a number of important applications such as ozone synthesizing, UV lamp house, CO2 lasers, et al. In recent years, due to its potential applications in plasma chemistry, semiconductor etching, pollution control, nanometer material and large area flat plasma display panels, DBD has received intensive attention from many researchers and is becoming a hot topic in the field of non-thermal plasma.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1979년도 제13차 학술대회 연제순서 및 초록
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• pp.8.2-8
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• 1979

상부기도가 갑자기 폐쇄증을 일으켜 심한 호흡곤란증을 호소하는 환자에 대하여 응급으로. 기도 및 호흡을 재확보하여야하며 이와같은 예를 임상에서 가끔 직면하게 된다. 이러한 환자에게 적절한 기도확보는 생명을 유지시킬 수 있다. 저자는 토끼를 대상으로 기존 기관의 직경을(약 3.4mm) 약 1/3(1.2mm), 1/4(0.8mm) 및 1/6(0.6mm)로 협소시켜 생리적 변화를 추적하였다. 결과는 다음과 같았다. 1) 혈액가스의 분석결과 직경을 약 1/3로 감소시켰던 군에서 생리적 변동이 별로 없었다. 2) 직경을 약 1/4, 1/6로 감소시켰던 군에서는 $PaO_2,$ $PaCO_2$ 및 pHa에 뚜렷한 변화를 나타내어 저산소혈증, 과탄산혈증 및 대사성산증을 나타내었다. 3) 호흡저항은 모든 군에서 뚜렷하게 증가하여 1회 호흡량도 현저하게 감소하였는데 16G(직경 1.2mm 호흡로)에서는 호흡수의 증가로 폐포환기가 적당하게 영위됨에 따라서 혈액가스 및 vital signs의 변동이 별로 없던 것으로 보아 토끼에서는 이 정도의 호흡로 폐쇄에는 1시간까지 견딜 수 있음을 알았다.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.241-247
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• 2016

Natamycin is a widely used antifungal antibiotic. For natamycin biosynthesis, the gene pimE encodes cholesterol oxidase, which acts as a signalling protein. To confirm the positive effect of the gene pimE on natamycin biosynthesis, an additional copy of the gene pimE was inserted into the genome of Streptomyces gilvosporeus 712 under the control of the ermE* promoter (p_ermE*) using intergeneric conjugation. Overexpression of the target protein engendered 72% and 81% increases in the natamycin production and cell productivity, respectively, compared with the control strain. Further improvement in the antibiotic production was achieved in a 1 L fermenter to 7.0 g/l, which was a 153% improvement after 120 h cultivation. Exconjugants highly expressing pimE and pimM were constructed to investigate the effects of both genes on the increase of natamycin production. However, the co-effect of pimE and pimM did not enhance the antibiotic production obviously, compared with the exconjugants highly expressing pimE only. These results suggest not only a new application of cholesterol oxidase but also a useful strategy to genetically engineer natamycin production.

• 융합정보논문지
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• 제10권11호
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• pp.16-22
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• 2020

개인 전력 생산자 및 기반 지식이 부족한 비전문가의 경우 EMS(Energy Management System)을 통해 시설을 제어, 관리, 운영하기 어렵기 때문에 증강현실 및 가상현실을 적용한 모니터링 시스템이 적용되고 있다. 그러나 기존의 시스템들은 센서에서 수집된 아날로그 신호 값에 대한 컬럼 값을 분석하고 이에 대한 컬럼들을 결합한 후 데이터를 변환하는 과정으로 인하여 데이터 엑세스 효율성이 떨어진다. 그리고 다양한 아날로그 신호 파형에 대한 액세스 패턴을 수용하기 위한 다수의 인덱스들로 인하여 고속 연산 처리가 어렵다. 따라서 본 논문에서는 전력설비에서 수집된 데이터를 비트맵 생성기(Bitmap Generator)를 비-트리 구조에 삽입하여 물성 정보로 변환하고 변환된 정보를 공통 키로 암호화하여 각 기기 사이에서 공유되는 리소스에 대한 자원을 계측적으로 제어하는 증강현실 기반 전력시스템의 데이터 교환방법을 제안한다.

• 한국전자파학회논문지
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• 제22권2호
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• pp.140-147
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• 2011

본 논문에서는 차량 후면 유리에 여러 주파수 대역의 안테나를 집적화할 수 있는 유리접지를 이용한 안테나 급전 방법을 제안하였다. 유리접지가 가능하도록 동축 선로를 유리접지면에 직접 연결할 수 있는 어댑터를 설계하였으며, 차체 접지 방식과 반사 손실을 비교 분석하였다. Azimuth 방향에서 높은 복사 이득을 얻기 위해 유리 접지의 크기와 위치를 최적화 하였으며, 도출된 유리접지면을 이용하여 삼각 패치 형상의 WiBro 안테나를 상용세단의 후면 유리에 인쇄하고 반사 손실과 복사 패턴 성능을 측정하였다. 제안된 유리접지를 이용한 급전 방식은 차체 접지를 이용한 급전 방식과 유사한 반사 손실을 보이며, 2 dB 높은 azimuth 복사 이득이 나타내었다. 측정 결과, 제안된 유리접지면을 이용한 안테나 급전 방식을 이용하면 여러 주파수에서 동작하는 다양한 형태의 온-글래스 안테나를 후면 유리에 효과적으로 적용할 수 있음을 확인하였다.

• 농업과학연구
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• 제44권1호
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• pp.30-39
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• 2017

This study was performed to produce succinic acid from biomass by developing mutants of Cellulomonas flavigena in which the succinate dehydrogenase gene (sdh) is deleted. For development of succinate producing mutants, the upstream and downstream regions of sdh gene from C. flavigena and antibiotic resistance gene (neo, bla) were inserted into pKC1139, and the recombinant plasmids were transformed into Escherichia coli ET12567/pUZ8002 which is a donor strain for conjugation. C. flavigena was conjugated with the transformed E. coli ET12567/pUZ8002 to induce the deletion of sdh in chromosome of this bacteria by double-crossover recombination. Two mutants (C. flavigena H-1 and H-2), in which sdh gene was deleted in the chromosome, were constructed and confirmed by PCR. To estimate the production of succinic acid by the two mutants when the culture broth was fermented with biomass such as CMC, xylan, locust gum, and rapeseed straw; the culture broth was analyzed by HPLC analysis. The succinic acid in the culture broth was not detected as a fermentation products of all biomass. One of the reasons for this may be the conversion of succinic acid to fumaric acid by sdh genes (Cfla_1014 - Cfla_1017 or Cfla_1916 - Cfla_1918) which remained in the chromosomal DNA of C. flavigena H-1 and H-2. The other reason could be the conversion of succinyl-CoA to other metabolites by enzymes related to the bypass pathway of TCA cycle.