• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.245-253
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• 2014

BACKGROUND: Oyster shell(OS) is alkaline with pH 9.8, porous, and has high concentration of $CaCO_3$. It could be used as an alternative of lime fertilizer to immobilize cadmium(Cd) in heavy metal contaminated arable soil. Therefore, this study has been conducted to compare effects of calcium(Ca) materials [OS and $Ca(OH)_2$] on Cd extractability in contaminated soil and determined mechanisms of Cd immobilization with OS. METHODS AND RESULTS: Both Ca materials were added at the rates of 0, 0.1, 0.2, 0.4, and 0.8% (wt Ca wt-1) in Cd contaminated soil and the mixtures were incubated at $25^{\circ}C$ for 4 weeks. Both Ca materials increased pH and negative charge of soil with increasing Ca addition and decreased 1N $NH_4OAc$ extractable Cd concentration. 0.1 N HCl extractable Cd concentration markedly decreased with addition of OS. 1 N $NH_4OAc$ extractable Cd concentration was related with pH and net negative charge of soil, but not with 0.1 N HCl extractable Cd concentration. We assumed that Cd immobilization with $Ca(OH)_2$ was mainly attributed to Cd adsorption resulted from increase in pH-induced negative charge of soil. Scanning electron microscope (SEM) images and energy dispersive spectroscopy(EDS) analyses were conducted to determine mechanism of Cd immobilization with OS. There was no visible precipitation on surface of both Ca materials. However, Cd was detected in innerlayer of OS by EDS analyses but not in that of $Ca(OH)_2$. CONCLUSION: We concluded that Cd immobilization with OS was different from that with $Ca(OH)_2$. OS might adsorbed interlayer of oyster shell or have other chemical reactions.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.6
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• pp.1393-1403
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• 2003

The genetic effects of restraint stress challenge on HPA axis and the therapeutic effect of Boshimgeonbi-Tang on the stress were studied with cDNA microarray analyses on hypothalamus using an immobilization-stress mouse as stress model. Male CD-1 mice were restrained in a tightly fitted and ventilated vinyl holder for 2hours once a day, and this challenge was repeated for seven consecutive days. The body weights of the immobilization-stress mice were diminished about 25 percent degree as compared to normal ones. Seven days later, total RNA was extracted from the organs of the mouse, body-labeled with CyDye/sup TM/ fluorescence dyes (Amersham Bioscience Co., NJ), and then hybridized to cDNA microarray chip. Scanning and analyzing the array slides were carried out using GenePix 4000 series scanner and GenePix Pro/sup TM/ analyzing program, respectively. The expression profiles of 109 genes out of 6000 genes on the chip were significantly modulated in hypothalamus by the immobilization stress. Energy metabolism-, lipid metabolism-, apoptosis- and signal transduction-related genes were transcriptionally activated whereas DNA repair-, protein biosynthesis-, and structure integrity-related genes were down-regulated in hypothalamus. The 58 genes were up-regulated by the mRNA expression folds of 1.5 to 7.9. and the 51 genes were down-regulated by 1.5 - 3.5 fold. The 20 genes among them were selected to confirm the expression profiles by RT-PCR. The mRNA expression levels of Tnfrsf1a (apoptosis), Calm2 (cell cycle), Bag3 (apoptosis), Hspe1 (protein folding), Aatk (apoptosis), Dffa (apoptosis), Itgb1 (cell adhesion), Vcam1 (cell adhesion), Fkbp5 (protein folding), BDNF (neuron survival) were restored to the normal one by the treatment of Boshimgeonbi-Tang.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.255-264
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• 2023

Background: Red ginseng (RG) alleviates psychiatric disorders. Fermented red ginseng (fRG) alleviates stress-induced gut inflammation. Gut dysbiosis causes psychiatric disorders with gut inflammation. To understand the gut microbiota-mediated action mechanism of RG and fRG against anxiety/depression (AD), we investigated the effects of RG, fRG, ginsenoside Rd, and 20(S)-β-D-glucopyranosyl protopanaxadiol (CK) on gut microbiota dysbiosis-induced AD and colitis in mice. Methods: Mice with AD and colitis were prepared by exposing to immobilization stress (IS) or transplanting the feces of patients with ulcerative colitis and depression (UCDF). AD-like behaviors were measured in the elevated plus maze, light/dark transition, forced swimming, and tail suspension tests. Results: Oral gavage of UCDF increased AD-like behaviors and induced neuroinflammation, gastrointestinal inflammation, and gut microbiota fluctuation in mice. Oral administration of fRG or RG treatment reduced UCDF-induced AD-like behaviors, hippocampal and hypothalamic IL-6 expression, and blood corticosterone level, whereas UCDF-suppressed hippocampal BDNF⁺NeuN⁺ cell population and dopamine and hypothalamic serotonin levels increased. Furthermore, their treatments suppressed UCDF-induced colonic inflammation and partially restored UCDF-induced gut microbiota fluctuation. Oral administration of fRG, RG, Rd, or CK also decreased IS-induced AD-like behaviors, blood IL-6 and corticosterone and colonic IL-6 and TNF-α levels, and gut dysbiosis, while IS-suppressed hypothalamic dopamine and serotonin levels increased. Conclusion: Oral gavage of UCDF caused AD, neuroinflammation, and gastrointestinal inflammation in mice. fRG mitigated AD and colitis in UCDF-exposed mice by the regulation of the microbiota-gut-brain axis and IS-exposed mice by the regulation of the hypothalamic-pituitary-adrenal axis.

• Bulletin of the Korean Chemical Society
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• v.26 no.11
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• pp.1839-1842
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• 2005

• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.193-196
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• 2004

The peptide Arg-Gly-Asp (RGD) was immobilized through their amino terminus to ends of a sugar bearing copolymer, producing a functional hybrid copolymer. Poly(N-p-vinylbenzyl-D-maltonamide-co-6-(p-vinylbenzamido)-hexanoic acid-g-GRGDS) [p(VMA-co-VBGRGDS)] promoted the attachment and growth of NIH fibroblast cells. The interaction between fibroblast cells and p(VMA-co- VBGRGDS) copolymer resulted in effective cell attachment, proliferation, and morphological changes by introduction of a GRGDS sequence. Moreover, when pretreated with soluble RGD monomer, attachment of fibroblast cells was suppressed approximately 50% from that of the p(VMA-co-VBGRGDS) surface.

• Bulletin of the Korean Chemical Society
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• v.31 no.10
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• pp.2973-2979
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• 2010

The newly synthesized homogeneous chiral Co(III) salen complexes were anchored non-covalently on the acidic sites of mesoporous Al-SBA-15. The Bronsted and Lewis acidic sites are attributed to the immobilization of fluorine functionalized chiral salen complexes on the supports. XRD, BET, TEM, FT-IR and ESCA (XPS) analyses were performed to characterize the property of support, and the structure of new homogeneous and heterogeneous chiral Co salen catalyst. The homogeneous and heterogeneous catalysts could be applied in asymmetric ring opening of epichlorohydrine (ECH) by water. They showed very high enantioselectivity and a good yield up to 99% in the catalytic synthesis of optically active products.

• Journal of Korean Society of Forest Science
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• v.69 no.1
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• pp.13-18
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• 1985

Forest soils mixed with organic matters (green needle, flesh needle litter and needle litter in F layer of Pinus koraiensis, and green leaf of Quercus dentata and Q. variabilis) were incubated under a constant $30^{\circ}C({\pm}1)$ for 53 days to measure the changes of inorganic nitrogen and $CO_2$ evolution rate. The results obtained were summarized as follows; 1) In the early incubation period the amounts of total inorganic nitrogen in soils by mixture of organic matters decreased rapidly because of immobilization by microbial uptake, and thereafter their amounts increased with further incubation. 2) The rate of immobilization of organic nitrogen in mixed organic matters was the highest in green needle among green needle, flesh needle litter and needle litter in F layer of P. koraiensis, but lower than that of green leaf of Q. variabilis and Q. dentata. 3) The rates of $CO_2$ evolution from soils mixed with organic matters increased sharply in the early time, and then decreased slowly with increasing time. The order of the $CO_2$ evolution rate was green leaf of Q. variabilis > green leaf of Q. dentata > green needle of P. koraiensis > flesh needle litter of P. koraiensis > needle litter of P. koraiensis in F layer from the largest to the least. 4) Nitrate nitrogen concentrations showed a tendency to increase throughout incubation time, so that their concentrations after 53 days were higher than that of ammonium nitrogen.

• Proceedings of the KOSOMBE Conference
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• v.1998 no.11
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• pp.223-224
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• 1998

RGDF immobilized micro-patterned surface was developed to detect the functional state of platelets. Using photolithographic technology, an RGDF micro-patterned surface was prepared on silicon wafer. Platelet adhesion to this surface was observed by fluorescence microscopy after staining platelets with mepacrine. Nonactivated platelets pretreated with $PGE_1$ interacted incompletely with the RGDF micro-patterned surface, whereas activated platelets treated with ADP interacted with the surface extensively. These results show that the distinct selectivity of an RGDF-immobilized micro-patterned surface can be used to detect the functional state of platelets.

• Journal of the Korean Society of Food Culture
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• v.2 no.1
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• pp.9-16
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• 1987

In early 1956, MSG (monosodium glutamate) had been produced by hydrolysis of the vegetable proteins in Korea. In accordance with development of fermentation technology mainly led by the Japanese scientists, its major production method has been changed to microbial fermentation since 1962. Meanwhile, 5'-ribonucleotides which are nucleic acid-related condiments have been produced by the enzymic hydrolysis of yeast RNA and/or the direct fermentation by Miwon Co. and Cheil sugar Co., respectively since 1977. At the technological viewpoints, Korean fermentation level seems relatively highly-reputated over the world in terms of production yield and unit-consumption level. For further progress of technology, our emphasis on this research area should be laid on both improvement of bacterial strain by means of modern biotechnology and process development through the immobilization and/or computerized control technics, etc.

• Applied Chemistry for Engineering
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• v.27 no.3
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• pp.239-244
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• 2016

The catalytic applicability of various ionic liquids immobilized on different silica-supports such as amorphous, SBA, MCM and commercial silica for the cycloaddition of $CO_2$ and epoxides is reviewed in this work. The effects of different structures of supported ionic liquids and silica supports in the synthesis cyclic carbonate by the cycloaddition of $CO_2$ have been remarked. The studies revealed that ionic liquids possessing functional groups or metals exhibited increased catalytic performance towards cyclic carbonate synthesis. Moreover, the reusability of SSIL catalyst and mechanism for the cycloaddition of $CO_2$ were studied.