• Radiation Oncology Journal
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• 제14권4호
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• pp.265-279
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• 1996

방사선은 DNA손상을 초래하고 세포 성장에 관련된 유전자의 발현 조절 및 apoptosis등을 유발한다고 알려져 있으며 본 연구는 신경계에 있어 방사선 조사 후 종양 발생율과 시간 경과의 관계 및 조사 양과 암 발생의 관계를 구명하기 위해 코발트 60의 전신조사에 따른 흰쥐 뇌 조직의 생체 반응을 연구하고자 하였다. 이를 위하여 상기조직의 jun 및 p53 유전자의 발현도를 1 Gy로 부터 100 Gy 범위의 감마선 용량별 및 1시간에서 6시간까지의 조사 훈 경과 시간 별로 Northern 분석하였다. Jun유전자 발현도는 ley이하에서 1시간 이내에 한계수준에 도달하였으며 30 Gy의 조사 1시간 째에 최대였다. 또한 조사 1시간 이후 1 Gy로부터 10 Gy 범위에서는 조사 5시간 및 6시간까지 점진적으로 증가되었으나 20 Gy로부터 100 Gy 범위에서는 조사 2시간까지 증가 후 감소되는 양상을 나타냈다. p53유전자의 발현도는 1 Gy이하에서 1시간 이내에 한계 수준에 근접했고 1 Gy의 조사 후 6시간 째에 최대였다. 1 Gy로부터 40 Gy까지의 범위에서는 조사 5시간 및 6시간까지 점진적으로 증가되는 반면 50 Gy에서 100 Gy범위에서는 조사 2시간 째까지 증가 후 감소되는 양상을 보였다. 따라서 감마선 조사양이 높을수록 jun 및 p53유전자는 신속하게 최대로 발현되었고 감마선 조사양이 낮을수록 서서히 증가되었다 그러나 jun유전자와 p53유전자의 감마선 조사에 따른 발현 양상에는 상호간의 연관성을 찾을 수 없었다.

• Journal of Radiation Protection and Research
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• 제40권1호
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• pp.25-35
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• 2015

We analyzed the differential effects of histopathology, apoptosis and expression of radiation response genes after chronic low dose rate (LDR) and acute high dose rate (HDR) radiation exposure in spleen, lung and liver of rats. Female 6-week-old Sprague-Dawley rats were used. For chronic low-dose whole body irradiation, rats were maintained for 14 days in a $^{60}Co$ gamma ray irradiated room and received a cumulative dose of 2 Gy or 5 Gy. Rats in the acute whole body exposure group were exposed to an equal dose of radiation delivered as a single pulse ($^{137}Cs$-gamma). At 24 hours after exposure, spleen, lung and liver tissues were extracted for histopathologic examination, western blotting and RT-PCR analysis. 1. The spleen showed the most dramatic differential response to acute and chronic exposure, with the induction of substantial tissue damage by HDR but not by LDR radiation. Effects of LDR radiation on the lung were only apparent at the higher dose (5 Gy), but not at lower dose (2 Gy). In the liver, HDR and LDR exposure induced a similar damage response at both doses. RT-PCR analysis identified cyclin G1 as a LDR-responsive gene in the spleen of rats exposed to 2 Gy and 5 Gy gamma radiation and in the lung of animals irradiated with 5 Gy. 2. The effects of LDR radiation differed among lung, liver, and spleen tissues. The spleen showed the greatest differential effect between HDR and LDR. The response to LDR radiation may involve expression of cyclin G1.

• 한국안전학회지
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• 제27권2호
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• pp.13-19
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• 2012

The effectiveness of treeing resistance in low density polyethylene containing azocompounds were investigated under radiation environments. 4-(4-nitrophenylazo)-1-naphthol and 4-(4-nitrophenylazo) resorcinol were selected for treeing inhibitors. LDPE containing the additives were crosslinked with dicumyl peroxide, irradiated by $Co^{60}$ ${\gamma}$-ray in the presence of air at room temperature. Treeing inception voltage, AC breakdown strength measurements were carried out for electrical insulation characteristics, thermoluminescence and gel contents were analyzed as various irradiation doses also. LDPE with treeing inhibitors showed much better insulation characteristics than pure LDPE. It is interpreted that molecular structures, polar groups and the type of radicals connected on the benzene ring of the additives affected electrical characteristics. From the results, 4-(4-nitrophenylazo) resorcinol was the most effective upon the best treeing and radiation resistance.

• 방사선산업학회지
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• 제5권4호
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• pp.305-311
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• 2011

We introduced a novel sustainable slow-releasing agrochemical formulation, a biopolymer bound to silica, for controlling plant diseases. The formulation was obtained through the following process. Curdlan, sodium silicate ($Na_2SiO_3$) and isopropyl alcohol were dissolved in DDW (Deionized-distilled water). The resultant solution was then irradiated using a $^{60}Co$ ${\gamma}$-irradiator (150 TBq of capacity; ACEL, Canada) at KAERI. The resultant solution was treated with phosphorous acid ($H_3PO_3$). Finally, we obtained a novel biopolymer-silica microsized formulation containing phosphorous acid ($H_3PO_3$) from the solution. The morphology of the complex was characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM and TEM images revealed that the curdlan-silica formulation has a particle size ranging from 1 to $3{\mu}m$ with high stability. We also detected that $H_3PO_3$ was distributed within the formulation through energy dispersive X-ray spectroscopy (EDX) analysis. $H_3PO_3$ was sustain-released from the formulation in water. Based on our results, it seems effectively that one or two applications of the formulation during a cropping season will assist in controlling various plant diseases.

• Structural Engineering and Mechanics
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• 제85권1호
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• pp.29-53
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• 2023

High-strength shielding concrete against gamma radiation is a priority for many medical and industrial facilities. This paper aimed to investigate the gamma-ray shielding properties of high-strength hematite concrete mixed with silica fume (SF) with nanoparticles of lead dioxide (PbO₂), tungsten oxide (WO₃), and bismuth oxide (Bi₂O₃). The effect of mixing steel fibres with the aforementioned binders was also investigated. The reference mixture was prepared for high-strength concrete (HSCC) containing 100% hematite coarse and fine aggregate. Thirteen mixtures containing 5% SF and nanoparticles of PbO₂, WO₃, and Bi₂O₃ (2%, 5%, and 7% of the cement mass, respectively) were prepared. Steel fibres were added at a volume ratio of 0.28% of the volume of concrete with 5% of nanoparticles. The slump test was conducted to workability of fresh concrete Unit weight water permeability, compressive strength, splitting tensile strength, flexural strength, and modulus of elasticity tests were conducted to assess concrete's engineering properties at 28 days. Gamma-ray radiation of 137Cs emits photons with an energy of 662 keV, and that of 60Co emits two photons with energies of 1173 and 1332 keV were applied on concrete specimens to assess radiation shielding properties. Nanoparticles partially replacing cement reduced slump in workability of fresh concrete. The compressive strength of mixtures, including nanoparticles was shown to be greater, achieving 94.5 MPa for the mixture consisting of 7.5 PbO2. In contrast, the mixture (5PbO₂-F) containing steel fibres achieved the highest values for splitting tensile, flexural strength, and modulus of elasticity (11.71, 15.97, and 42,840 MPa, respectively). High-strength shielded concrete (7.5PbO2) showed the best radiation protection. It also showed the minimum concrete thickness required to prevent the transmission of radiation.

• 방사선산업학회지
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• 제4권4호
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• pp.347-352
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• 2010

In this study, we addressed a novel nanosized curdlan-silica complex, which is curdlan bound to silica, for the development of a sustain-releasing micro element fertilizer formulation. The complex was obtained as follow steps; First, Curdlan polymer, sodium silicate ($Na_2SiO_3$) and isopropyl alcohol were dissolved in DDW. Next the resultant solution was irradiated by $^{60}Co$ gamma-irradiator (150 TBq of capacity; ACEL, Canada). Then $MgSO_4$ was treated with the resultant solution. The obtained colloidal solution was dried by freeze dryer. Finally, we obtained a novel nanosized curdlan-silica formulation containing $MgSO_4$ from the colloidal solution. The morphology of the complex was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The nanosized curdlan-silica complex has a particle size ranging from 20 to 80 nm and high stability. Our results suggested that the nano-complex can be applicable to use in various sustain-releasing formulation for pesticide delivery system (PDS).

• Applied Biological Chemistry
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• 제10권
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• pp.63-68
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• 1968

고구마 저장중의 성분변화(成分變化), 연부병(軟腐病)에 대한 감수성(感受性) 및 연부병균포자(軟腐病菌胞子)의 치사(致死)에 미치는 방사선(放射線)($^{60}Co-{\gamma}$)의 영향을 조사하여 다음과 같은 결과를 얻었다. 1) $^{60}Co-{\gamma}$선조사(線照射)에 의하여 고구마의 수용성당(水溶性糖)은 증가하고 환원형(還元型) ascorbic acid는 현저히 감소하였다. 2) 조사선량(照射線量)이 높을수록 直後)의 호흡율(呼吸率)은 증가하나 시간이 경과됨에 따라 고선량(高線量)의 조사구(照射區)에서는 조직(組織)의 갈변(褐變)과 아울려 호흡율(呼吸率)이 감소하였다. 3) 고선량(高線量)($2.0{\times}10^5$ rad 이상)의 조사(照射)는 고구마 저장(貯藏)중 냉해(冷害)의 영향과 같이 연부병균(軟腐病菌)의 감염율(感染率)을 증가시켰다. 4) 고구마연부병균(軟腐病菌)인 Rhizopus nigricans 포자(胞子)의 평균치사선량(平均致死線量)은 $3{\times}10^5$ rad이며 $1.25{\times}10^5$ rad에서 완전사멸(完全死滅)되었다.

• 미생물학회지
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• 제38권3호
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• pp.162-167
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• 2002

항진균 활성 관련 단백질을 찾기 위해 미역류로부터 Streptomyces sp. SAR01을 분리하고, FAME (fatty acid methyl ester)분석으로 동정하였다. 감마선(sup $({60}^Co)$)조사를 이용하여 항진균 활성 결핍 돌연변이체(SAR535)를 유도한 후, 이차원전기영동으로 단백질을 분석한 결과 SAR01에만 존재하는 6종의 단백질을 확인할 수 있었다. 이중 10 kDa chaperonin cpn10 (GroES)과 96%의 상동성을 가진 10 kDa의 단백질을 클로닝하였으며, E. coli Ml5에서 과대 발현됨을 확인하였다. 또한 SAR535에 형질전환시킨 결과 SAR01과 유사한 항진균 활성이 나타났다. 이것으로 볼 때 groES는 Streptomyces sp. SAR01의 항진균 활성에 관련된 것으로 사료된다.

• Nuclear Engineering and Technology
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• 제31권4호
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• pp.423-431
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• 1999

A radiotracer technique has been developed to monitor the rotational movement of two piston rings in one cylinder during engine operation. The rings were labeled with two different kinds of radioisotopes, i.e. $^{60}$ Co and $^{192}$ Ir, for identification of the top ring from the second ring. The radiotracers were implanted in a small hole bored on the inner side of each piston ring. The rings were installed in a single cylinder hydrogen engine and three Nal scintillation detectors were mounted around the engine block to measure the gamma radiation. The angle of ring-gap orientation was determined from the radiation counts measured with the three detectors during engine operation. Two windows (upper window for $^{60}$ Co and lower window for $^{192}$ Ir) were set on each ratemeter to count radiation from the two isotopes separately. Procedure to convert the radiation counts to the position of the ring gap was established. With the software programmed with MS-Visualbasic, radiation counts were compared with the reference responses that were measured at angular intervals of 10$^{\circ}$for each piston ring in advance of the experiment. The result was used for the evaluation of the relationship between the orientation of ring-gaps and oil consumption. It was found that an increase in the oil consumption rate of a specific operation condition was closely related to the relative phase angle of the two piston rings.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 하계학술대회 논문집 D
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• pp.2285-2288
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• 2002

Recently, electronic personal dosimeters based upon silicon PIN photodiode or miniature GM tube were developed and have attracted a lot of attention because of the advantages of their nature such as indication of dose rate and the cumulative dose, and facilitation of record keeping. In this paper, we have developed a high-sensitivity electronic personal dosimeter with silicon PIN photodiode. The electronic personal dosimeter is constructed with silicon PIN photodiode, preamplifier, and shaping amplifier. To show the effectiveness of electronic personal dosimeter, we conducted nuclear radiation experiments using $\gamma$-ray Ba-133, Cs-137, and Co-60. The electronic personal dosimeter have a good linearity on $\gamma$-ray energy and activity.