• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.12
• /
• pp.7489-7496
• /
• 2013

This study was conducted to investigate enhancement of anti-tumor effects of the lentogenic Newcastle disease virus Clone30 strain (NDV rClone30) expressing cytosine deaminase (CD) gene against tumor cells and in murine groin tumor-bearing models. Cytotoxic effects of the rClone30-CD/5-FC on the HepG2 cell line were examined by an MTT method. Anti-tumor activity of rClone30-CD/5-FC was examined in H22 tumor-bearing mice. Compared to the rClone30-CD virus treatment alone, NDV rClone30-CD/5-FC at 0.1 and 1 MOIs exerted significant cytotoxic effects (P<0.05) on HepG2 cells. For treatment of H22 tumor-bearing mice, recombinant NDV was injected together with 5-FC given by either intra-tumor injection or tail vein injection. When 5-FC was administered by intra-tumor injection, survival for the rClone30-CD/5-FC-treated mice was 4/6 for 80 days period vs 1/6, 0/6 and 0/6 for the mice treated with rClone30-CD, 5-FC and saline alone, respectively. When 5-FC was given by tail vein injection, survival for the rClone30-CD/5-FC-treated mice was 3/6 vs 2/6, 0/6 and 0/6 for the mice treated with rClone30-CD, 5-FC or saline alone, respectively. In this study, NDV was used for the first time to deliver the suicide gene for cancer therapy. Incorporation of the CD gene in the lentogenic NDV genome together with 5-FC significantly enhances cell death of HepG2 tumor cells in vitro, decreases tumor volume and increases survival of H22 tumor-bearing mice in vivo.

• Journal of Korean Society of Forest Science
• /
• v.36 no.1
• /
• pp.1-4
• /
• 1977

The variation of isoperoxidase band patterns in the zymograms in the leaves of ${\times}$P. albaglandulosa clones showing excellent growth were observed by starch gel electrophoresis in this study. The results are summerized as follows; The numbers of total bands in the clones were six to eleven. Four to seven were active and one to four were of trace in these bands, and also active bands appeared plentifully in all clones. The appearing pattern of the bands was more monotonous to the cathode than to the anode. Besides, the uniqueness of the isoenzyme forms in each clone made possible to identify the clones, and g and 1 bands were fixed in ${\times}$P. albaglandulosa, ${\times}$P. albaglandulosa being $F_1$ hybrid, the genetic variation of isoenzyme forms was significant statistically.

• Korean Journal of Microbiology
• /
• v.25 no.3
• /
• pp.198-204
• /
• 1987

Biparental clones and recombinant clones were obtained by spheroplast fusion of Pseudomonas putida KU218R-3 and P.putida KU428. Formation of the fusion product was the most effective when the Pseudomonas spheroplast mixture were treated with 40% plyethyleneglycol(PEG) 6000 for 10min at room temperature, The fusants which selected by indirect method were obtained at an average frequency of 10.8%. Most of the fusants were biparental clones (10.4%) and the recombinant clones were produced in low yield (0.42%). Fusants, at the frequency of 4% were obtained without PEG 6000, which shows that fusion is not strictly dependant on PEG. The stability of fusants were examined. Most of the biparental clones were segregated to parental form amd late recombinants were formed on further propagation of biparental clone but the recombinant clones were nery stable.

• Journal of Software Assessment and Valuation
• /
• v.7 no.1
• /
• pp.29-39
• /
• 2011

To evaluate the quality of clone detection tools, we should know how many clones the tool misses. Hence we need to have the standard code-clone reference corpus for a carefully chosen set of sample source codes. The reference corpus available so far has been built by manually collecting clones from the results of various existing tools. This paper presents a tree-pattern-based clone detection tool that can be used for automatic generation of reference corpus. Our tool is compared with CloneDR for precision and Bellon's reference corpus for recall. Our tool finds no false positives and 2 to 3 times more clones than CloneDR. Compared to Bellon's reference corpus, our tools shows the 93%-to-100% recall rate and detects far more clones.

• Development and Reproduction
• /
• v.4 no.2
• /
• pp.147-152
• /
• 2000

Four clonal lines of ayu, Plecoglossus altivelis, were produced through gynogenesis, mixed before hatching and reared communally. After 10 months, a randomly taken sample was subjected to a standardized shallow water stressor. Hematocrit, hemoglobin, red blood cells count (RBC) and mean corpuscular volume (MCV) were obtained from stressed and non-stressed fish. DNA fingerprinting was used to confirm the clonal nature of the organisms and to identified the clonal line to which each fish belonged. 1 observed significant differences between clona] lines mostly in the hematocrit and MCV measured under no-stress conditions. Such differences are suggested to represent mainly genetic variance, on account of the common environment provided to all the experimental groups. The stress response ratio was lower than expected, mainly due to some unexpectedly high non-stress values. Heritability values (h$^2$) were medium to high for the no-stress measurements (mean 0.238) and very low or zero for the stressed groups'traits (excepting one high value of 0.484). 1 conclude that the use of communally reared clonal lines represents a good tool for the characterization of the physiological traits, thus allowing for their utilization as genetic selection criteria.

• Applied Biological Chemistry
• /
• v.38 no.1
• /
• pp.49-54
• /
• 1995

To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses, we have isolated cDNA clones for garlic viruses. The partial nucleotide sequences of 24 cDNA clones were determined and those of five clones containing poly(A) tail were compared with sequences of other plant viruses. One of these clones, V9, has a primary structure similar to the carlavirus group, suggesting that the clone V9 derived from a part of garlic latent virus (GLV). Northern blot analysis with the clone V9 as a probe demonstrated that GLV genome is 8.5 knt long and has a poly(A) tail. The clone V9 encodes coat protein (CP) of 33 kDa and nucleic acid binding protein of 10 kDa in different reading frame. The hexanucleotide motif, 5'-ACCUAA, which is conserved in the 3' noncoding region arid was proposed to be a cis-acting element involved in the production of negative strand genomic RNA was noticed. Complementary sequence to the hexanucleotide motif, 5'-TTAGGT, is also found in the positive strand of V9 RNA. The putative CP gene was cloned into the pRSET-A expression vector and expressed in E. coli BL21. The expressed recombinant V9CP protein was purified by $Ni^{2+}$ NTA affinity chromatography. The anti-V9CP antibody recognizes 34 kDa polypeptide which could be CP of GLV in infected garlic leaf extract. Immunoblot and Northern blot analysis of various cultivars shows wide occurrence of GLV in Korean garlic plants.

• Plant Resources
• /
• v.4 no.3
• /
• pp.111-120
• /
• 2001

The efficiency of in vitro regeneration of four clones of Populus euramericana, Canada blanc, Eugenii, I-45/51, and Wisconsin #5, was examined. Cytokinins and the combinations with auxins affected the rate of regeneration from the explants of root segments, stem internodes, and leaf discs. Overall, BA and the combination with auxins were effective in root segments and leaf discs of the Canada blanc clone, whereas zeatin and the combination with auxins were important in stem internodes of the Wisconsin #5 clone. The highest number of shoots averaging 17.6 $\pm$ 0.47 from root segments in the Canada blanc clone,18.2 $\pm$ 3.0 from stem internodes in the Wisconsin #5 clone, and 17.8 $\pm$ 1.92 from leaf discs in the Canada blanc clone were obtained with 2.0 mg/1 BA, 2.0 mg/l zeatin combined with 0.2 mg/l IAA, and 0.5 mg/l BA combined with 0.05 mg/l 2,4-D, respectively. In particular, the addition of 2,4-D into cytokinin medium promoted shoot proliferation.

• Journal of Microbiology
• /
• v.45 no.1
• /
• pp.1-5
• /
• 2007

The bacterial compositions between the dental unit water system and human saliva were characterized and compared by direct sequence analysis of 16S rDNA clone libraries. Based on the species richness estimation, bacterial diversity in the dental unit water system (DUW) was more diverse than that of the human saliva (HS). The Chaol estimates of species richness in HS and DUW samples were 12.0 and 72.4, respectively. The total numbers of OTUs observed in the combined libraries accounted for 83% (HS) and 59% (DUW) of the Chaol diversity estimate as defined at the 80% similarity threshold. Based on the sequence analysis, the phylum Proteobacteria was the major group in both clone libraries at phylum level. DUW clone library contained 80.0% Proteobacteria, 8.0% Bacteroides, 4.0% Nitrospira, 4.0% Firmicutes, 2.0% Planctomycetes and 2.0% Acidobacteria. On the other hand, human saliva (HS) clone library contained 55.5% Proteobacteria, 36.1% Firmicutes and 8.4% Bacteroides. The majority of bacteria identified belonged to phylum Proteobacteria in both samples. In dental unit water system (DUW), Alphaproteobacteria was detected as the major group. There was no evidence of the bacterial contamination due to a dental treatment. Most sequences were related to microorganisms derived from biofilm in oligotrophic environments.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.12 no.5
• /
• pp.1932-1950
• /
• 2018

The paper proposes a code-clone detection method that gives the highest possible precision and recall, without giving much attention to efficiency and scalability. The goal is to automatically create a reliable reference corpus that can be used as a basis for evaluating the precision and recall of clone detection tools. The algorithm takes an abstract-syntax-tree representation of source code and thoroughly examines every possible pair of all duplicate tree patterns in the tree, while avoiding unnecessary and duplicated comparisons wherever possible. The largest possible duplicate patterns are then collected in the set of pattern clusters that are used to identify code clones. The method is implemented and evaluated for a standard set of open-source Java applications. The experimental result shows very high precision and recall. False-negative clones missed by our method are all non-contiguous clones. Finally, the concept of neighbor patterns, which can be used to improve recall by detecting non-contiguous clones and intertwined clones, is proposed.