• 제어로봇시스템학회:학술대회논문집
• /
• 1990.10a
• /
• pp.559-563
• /
• 1990

A new speed improvement method for quantitative superimposed EMG signal analysis to diagnose the neuromuscular dysfunction is described. The improvement is achieved through the use of efficient software and hardware signal processing techniques. The software approch is composed of the MANDF filter and HRWA algorithm which provides the optimal set and time delays of-selected templates. The hardware employs a TMS32OC25 DSP chip to execute the intensive calculation part. The purposed method is verified through a simulation with real templates which are obtained from needle EMG. As a results, the proposed method provides an overall speed improvement of 32-40 times.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.33 no.5B
• /
• pp.379-385
• /
• 2008

For the sharing and exchange of information between medical clinics, the clinical document has to be built on a standardized protocol such as a HL7-CDA. But it is difficult to exchange information between medical clinics because clinical document such as electronic medical record that include text and image, have different structure of document and type of expression. In this paper, we propose the electronic medical record system based on HL7-CDA that can share and exchange clinical information between medical institute. For this purpose, we have to design the schema of the clinical document architecture after we select the essential items of medical record and define templates. The proposed system can minimize integrating process and save parsing time when clinical information exchange and refer, by converting electronic medical record to base64 encoding scheme and integrate it in a XML document.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.5
• /
• pp.745-752
• /
• 2015

Tuberculosis (TB) is the most common mycobacterial infection in developing countries, requiring a rapid, accurate, and well-differentiated detection/diagnosis. For the rapid detection and discrimination of Mycobacterium tuberculosis complex (MTC) from non-tuberculous mycobacteria (NTM), a novel, simple, and primer-combined single-step multiplex PCR using three primer pairs (6110F-6110R, 1081F-1081R, and 23SF-23SR; annealing on each of IS6110, IS1081, and 23S rDNA targets), hereafter referred to as a triplex PCR, has been developed and evaluated. The expected product for IS6110 is 416 bp, for IS1081 is 300 bp, and for 23S rDNA is 206 bp by single PCR, which was used to verify the specificity of primers and the identity of MTC using DNA extracted from the M. tuberculosis H37Rv reference strain (ATCC, USA) and other mycobacteria other than tuberculosis (MOTT) templates. The triplex PCR assay showed 100% specificity and 96% sensitivity; the limit of detection for mycobacteria was ~100 fg; and it failed to amplify any target from DNA of MOTT (50 samples tested). Of 307 blinded clinical samples, overall 205 positive M. tuberculosis samples were detected by single PCR, 142 by conventional culture, and 90 by AFB smear methods. Remarkably, the triplex PCR could subsequently detect 55 positive M. tuberculosis from 165 culture-negative and 115 from 217 AFB smear-negative samples. The triplex PCR, targeting three regions in the M. tuberculosis genome, has proved to be an efficient tool for increasing positive detection/discrimination of this bacterium from clinical samples.

• Journal of KIISE:Databases
• /
• v.33 no.2
• /
• pp.239-249
• /
• 2006

Interoperability has been deemphasized from the hospital information system in general, because it is operated independently of other hospital information systems. This study proposes a future-oriented hospital information system through the design and actualization of the HL7 clinical document architecture. A clinical document is generated using the hospital information system by analysis and designing the clinical document architecture, after we defined the item regulations and the templates for the release form and radiation interpretation form. The schema is analyzed based on the HL7 reference information model, and HL7 interface engine ver.2.4 was used as the transmission protocol. This study has the following significance. First, an expansion and redefining process conducted, founded on the HL7 clinical document architecture and reference information model, to apply international standards to Korean contexts. Second, we propose a next-generation web based hospital information system that is based on the clinical document architecture. In conclusion, the study of the clinical document architecture will include an electronic health record (EHR) and a clinical data repository (CDR), and also make possible medical information-sharing among various healthcare institutions.

• Journal of Biomedical Engineering Research
• /
• v.27 no.3
• /
• pp.131-141
• /
• 2006

A visual decision by clinical experts like physical therapists is a best way to detect onset and offset time of muscle activation. The current computer-based algorithms are being researched toward similar results of clinical experts. The new algorithm in this paper has an ability to extract a trend from noisy input data. Kalman smoother is used to recognize the trend to be revealed from disorderly signals. Histogram of smoothed signals by Kalman smoother has a clear boundary to separate muscle contractions from relaxations. To verify that the Kalman smoother algorithm is reliable way to detect onset and offset time of muscle contractions, the algorithm of Robert P. Di Fabio (published in 1987) is compared with Kalman smoother. For 31 templates of subjects, an average and a standard deviation are compared. The average of errors between Di Fabio's algorithm and experts is 109 milliseconds in onset detection and 142 milliseconds in offset detection. But the average between Kalman smoother and experts is 90 and 137 milliseconds in each case. Moreover, the standard deviations of errors are 133 (onset) and 210 (offset) milliseconds in Di Fabio's one, but 48 (onset) and 55 (offset) milliseconds in Kalman smoother. As a result, the Kalman smoother is much closer to determinations of clinical experts and more reliable than Di Fabio's one.

• Korean Journal of Veterinary Research
• /
• v.43 no.1
• /
• pp.121-127
• /
• 2003

Ornithobacterium rhinotracheale (OR) is a bacterium responsible for a respiratory disease in turkeys and chickens, and has been identified as one of the emerging respiratory bacterial pathogens. Ten cases of four hundred cases submitted to National Veterinary Research & Quarantine Service for diagnosis in 2001 and in 2002 were diagnosed as OR infection. The major clinical signs of chickens infected with OR were respiratory symptoms including sneezing, sniveling, wet eyes, and swelling of the sinus infraorbitalis at 3 to 4 weeks of age. At necropsy, gross lesions were commonly found to foamish, white, and yoghurt-like exudates in the peritonium and abdominal air sacs. Microscopically, epithelial metaplasia and proliferation of air sacs were prominant with accompaning inflammatory reactions characterized by heterophils, fibrins, and bacterial colonization. Ten field isolates were obtained from air sacs and peritonium of these affected chickens, and were identified as OR, resulted from by gram-staining, catalase, oxidase, API NE and API ZYM Kit. In additon, using a previously reported primer targeted to 16S rRNA of ORT, 784bp fragment was successfully amplified from templates extracted from the isolates and a reference strain. This report describes an occurrence of Ornithobacterium rhinotracheale infection in chickens in Korea.

• The Journal of Korean Academy of Prosthodontics
• /
• v.46 no.6
• /
• pp.634-638
• /
• 2008

PURPOSE: The application of computer-aided technology to implant dentistry has created new opportunities for treatment planning, surgery and prosthodontic treatment, but the correct selection and combination of available methods may be challenging in times. Hence, the purpose of this case report is to present a combination of several computer-aided tools as approaches to manage complicated implant case. MATERIAL AND METHODS: A 47 year-old female patient with severe dental anxiety, high expectations, financial restrictions and poor compliance presented for a fixed rehabilitation. A CT scan with a radiographic template obtained with software (SimPlant, Materialize, Leuven, Belgium) was used for treatment planning. The surgical plan was created and converted into a stereolithographic model of the maxilla with bone-supported surgical templates (SurgiGuide, Materialise, Leuven, Belgium), that allowed for the precise placement of 7 implants in a severely resorbed edentulous maxilla. After successful osseointegration, an accurate scan model served as the basis for the fabrication of a one-piece milled titanium framework using the Procera (Nobel Biocare, Gothenburg, Sweden) technology. The final rehabilitation of the edentulous maxilla was rendered in the form of a screw-retained maxillary metal-reinforced resin-based complete prosthesis. RESULTS: Despite challenging circumstances, 7 implants could be placed without bone augmentation in a severely resorbed maxilla using the SimPlant software for pre-implant analysis and the SurgiGuide-system as the surgical template. The patient was successfully restored with a fixed full arch restoration, utilizing the Procera system for the fabrication of a milled titanium framework.

• Archives of Plastic Surgery
• /
• v.32 no.1
• /
• pp.1-4
• /
• 2005

Previous sucessful results of neocartilage formation using tissue engineering technique in immunocompromised nude mouse xenograft model were reported. For clinical application, autogenous cell is preferrable to allogenic or xenogenic cell for circumvention of immune rejection. This study evaluates the feasibility of producing a engineered cartilage using autogenous chondrocytes. Chondrocytes were isolated from the auricular catilage of New Zealand White rabbit and seeded onto PGA polymer coated with polylactic acid in round pattern(diameter 0.7 cm, thickness 0.1 cm) at a concentration $7{\times}10^7$ chondrocytes per $cm^3$. Each Autogenous Cell-polymer constructs were implanted subcutaneously into the left side of dorsum of twelve Rabbits. Polymer templates not containg cells were implanted into the right side as a control. Fifteen rabbits were sacrificed at the following intervals: 5 rabbits at nine weeks, 7 rabbits at twelve weeksNew autogenous cartilage formation which retained the approximate dimensions of origianl round polymer template in 11 of 12 cell seeded implants. Histological examination using hematoxyline and eosin stain revealed vast majority of implants developed into mature cartilage. This study opens up the possibility of autologus cell transplant to construct autogenous cartilge.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.6
• /
• pp.1164-1169
• /
• 2008

We developed multiplex RT-PCR assays that can detect and identify 12 hemagglutinin (H1-H12) and 9 neuraminidase (N1-N9) subtypes that are commonly isolated from avian, swine, and human influenza A viruses. RT-PCR products with unique sizes characteristic of each subtype were amplified by multiplex RT-PCRs, and sequence analysis of each amplicon was demonstrated to be specific for each subtype with 24 reference viruses. The specificity was demonstrated further with DNA or cDNA templates from 7 viruses, 5 bacteria, and 50 influenza A virus-negative specimens. Furthermore, the assays could detect and subtype up to $10^5$ dilution of each of the reference viruses that had an original infectivity titer of $10^6\;EID_{50}/ml$. Of 188 virus isolates, the multiplex RT-PCR results agreed completely with individual RT-PCR subtyping results and with results obtained from virus isolations. Furthermore, the multiplex RT-PCR methods efficiently detected mixed infections with at least two different subtypes of influenza viruses in one host. Therefore, these methods could facilitate rapid and accurate subtyping of influenza A viruses directly from field specimens.

• Journal of Microbiology
• /
• v.45 no.1
• /
• pp.21-28
• /
• 2007

In order to search for specific genotypes related to this unique phenotype, we used whole genomic DNA microarray to characterize the genomic diversity of Helicobacter pylori (H. pylori) strains isolated from clinical patients in China. The open reading frame (ORF) fragments on our microarray were generated by PCR using gene-specific primers. Genomic DNA of H. pylori 26695 and J99 were used as templates. Thirty-four H. pylori isolates were obtained from patients in Shanghai. Results were judged based on In(x) transformed and normalized Cy3/Cy5 ratios. Our microarray included 1882 DNA fragments corresponding to 1636 ORFs of both sequenced H. pylori strains. Cluster analysis, revealed two diverse regions in the H. pylori genome that were not present in other isolates. Among the 1636 genes, 1091 (66.7%) were common to all H. pylori strains, representing the functional core of the genome. Most of the genes found in the H. pylori functional core were responsible for metabolism, cellular processes, transcription and biosynthesis of amino acids, functions that are essential to H. pylori's growth and colonization in its host. In contrast, 522 (31.9%) genes were strain-specific genes that were missing from at least one strain of H. pylori. Strain-specific genes primarily included restriction modification system components, transposase genes, hypothetical proteins and outer membrane proteins. These strain-specific genes may aid the bacteria under specific circumstances during their long-term infection in genetically diverse hosts. Our results suggest 34 H. pylori clinical strains have extensive genomic diversity. Core genes and strain-specific genes both play essential roles in H. pylori propagation and pathogenesis. Our microarray experiment may help select relatively significant genes for further research on the pathogenicity of H. pylori and development of a vaccine for H. pylori.