• 대한임상검사과학회지
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• 제41권4호
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• pp.196-207
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• 2009

For the estimation of prevalence state of major chronic adult disease and their relationships with drinking and smoking habits in the Korean employees, we analyzed a medical check-up data of 155,799 subjects that was accumulated during the year of 2008. In age and sex distribution of the sample, male subjects were 106,229 and female 51,827 showing the ratio of 2:1 and the majorities were 30s and 40s covering 70.7% of the total. The prevalence rates of major chronic diseases were obesity 29.8% (male 38.3%, female 12.3%), hypertension 4.1%, HBV carrier inactive 3.3%, diabetes mellitus 2.9%, hypothyroidism 1.7% (male 1.3%, female 2.4%), hyperlipidemia 1.1%, hyperthyroidism 1.4% (male 1.1%, female 2.1%), osteoporosis 1.4% (male 1.4%, female 1.4%), anemia 0.9% (male 0.3%, female 2.0%) and renal disease 0.9%. The frequency of and volume of drinking in male group were 4.6 times and 7.5 times higher than female group respectively. The 33.8% of the workers were smoking currently. In the serological tests, all the items such as AST, ALT, ${\gamma}-GTP$, LDH for liver function, Cholesterol, TG, uric acid for hyperlipidemia and BP systolic, Fasting blood sugar, BMI for metabolic syndrome were significantly higher in the more drinking and more smoking groups than other groups (p<0.001). The higher prevalence rates in male group in the liver disease seems to be strongly related with the drinking and smoking habits in male employees. We suggest that employees should rather relying on leisure or hobbies than drinking and smoking for the stress relief.

• 대한임상검사과학회지
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• 제37권3호
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• pp.212-215
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• 2005

Carpal tunnel syndrome (CTS) is the most common entrapment neuropathy in clinical practice, with a 0.1% life time risk in the general population. Conventional neurophysiological studies have been useful in the diagnosis of this condition, as have a number of more specialized procedures. Therefore, we evaluated the diagnostic sensitivities of several parameters in nerve conduction technique for CTS patients. We analyzed 100 patients (159 hands) who were diagnosed with CTS clinically and electrophysiolosically. Median motor and sensory nerve conduction velocities (MCV and SCV) with wrist, palm, and finger stimulation were performed in traditional methods. Sensitivities of each test were calculated and compared to normal control data. The sensitivities of existing nerve conduction method were noted in terminal latency on median nerve, 2nd finger-wrist segment, 3rd finger-wrist segment, palm-wrist segment and distoproximal ratio, as 72.96%, 92.45%, 94.34%, 94.97%, and 97.48%, respectively. In the early course of CTS, sensory nerve conductions in the median nerve are more valuable than motor nerve conduction. Sensory nerve conductions are usually affected before motor nerve conductions in CTS. In this study, we detected that slowing of median SCV was the most frequent in the distoproximal ratio.

• 대한임상검사과학회지
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• 제53권1호
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• pp.11-18
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• 2021

호흡기 바이러스는 감염된 방문자와의 직접적인 접촉을 통해 병원 환경에서 감염된다. 감염관리 분야에서 호흡기 바이러스에 의한 병원내 획득 감염의 주요한 문제를 유발한다. 임상 검사실에서 파생된 감시 데이터는 또한 의료 서비스를 제공하는 기관과 공중 보건 분야에서 치료, 소모품 및 진단 제품 구매를 위해 병원과 지역 사회에 의료자원을 적절하게 배분하기 위해 종종 사용된다. 호흡기바이러스의 감염에서 조기 진단은 필수적이며 호흡기 검체를 사용하는 진단법에 활용될 수 있는 방법에는 바이러스 배양, 분자 진단 및 분석 등이 포함된다. 랩온어칩(LoC)/마이크로칩은 보다 다양하고 강력한 기술인 차세대 현장검사 시험법을 개발할 수 있는 새로운 전략으로 제공한다. 호흡기계의 중요성은 의료관련 종사자의 직업적 건강과 안전을 보장하기 위해 감염 관리 지침에 엄격히 적용되어야 한다. 이 연구를 포함하여 점점 더 많은 임상적 효능 증거가 감염 전파에 대한 오랜 패러다임에 도전하고 있다. 바이러스의 감염 가능성을 의심하는 새로운 호흡기 증상이 시작된 입원 환자로부터 호흡기 바이러스를 탐지하기 위한 빈번한 검사에 대해 추가 지원이 요구되고 감염통제의 노력에 집중적으로 도움이 이루어져야 할 것이다.

• 대한의생명과학회지
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• 제16권1호
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• pp.19-24
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• 2010

We analyzed and compared the concentration of total cholesterol (CHOL), high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol and triglyceride (TG) in serum and the serum protein electrophoresis fractions of thyroid disease patients. In comparison with the average of reference, our data showed that the average concentration of CHOL, LDL cholesterol and TG in hyperthyroidism patients were decreased significantly, but HDL cholesterol was increased significantly. In hypothyroidism patients, CHOL, HDL cholesterol, LDL cholesterol and TG were all increased significantly. In comparison of the concentration of lipids in each patient to reference range, 28.3% of hyperthyroidism patients showed abnormally low level of total cholesterol. In the patients with hypothyroidism, the percentage of patients showed abnormally high level of CHOL, HDL cholesterol, LDL cholesterol and TG were 37.7%, 10%, 68.8% and 49.1%, respectively. In our studies of serum protein electrophoresis, the average of ${\alpha}_2$-globulin and $\gamma$-globulin in hyperthyroidism patients were increased and $\beta$-globulin was decreased significantly. In hypothyroidism patients, the average of $\gamma$-globulin was increased and $\beta$-globulin was decreased significantly. In comparison of protein fractions of each patient to reference range, 38.3% and 50.0% of hyperthyroidism patients showed abnormally high levels of ${\alpha}_2$-globulin and $\gamma$-globulin, but 73.3% of patients showed abnormally low level of $\beta$-globulin. In hypothyroidism patients, 70.4% of patients were abnormally decreased in $\beta$-globulin and 63.9% of patients were abnormally increased in $\gamma$-globulin. These data suggest that the concentrations of CHOL, HDL cholesterol, LDL cholesterol and TG are not critical data for clinical interpretation of hyperthyroidism, but the levels of them are useful for interpretation of hypothyroidism patients. Our results of serum protein electrophoresis suggest that the concentration of serum protein electrophoresis fractions can be useful to understand the thyroid disease.

• 대한임상검사과학회지
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• 제52권3호
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• pp.202-213
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• 2020

미생물 연구에서 대량의 마이크로바이옴 데이터를 효율적으로 얻는 기술이 발전해왔지만, 마이크로바이옴 빅 데이터를 적절하게 분석하는 도구는 여전히 부족하다. 또한 빈약한 데이터베이스를 사용하여 미생물 군집을 분석하면 잘못된 결과를 초래할 수 있다. 따라서 본 연구는 대량의 미생물 데이터베이스 분석을 위한 적절한 방법을 설계하고자 하였다. 박테리아는 개인의 손끝과 개인 소지품(휴대 전화 및 랩탑 키보드)에서 수집되었다. 박테리아로부터 게놈 DNA를 추출하고 16S rRNA 유전자를 표적으로 하여 차세대 시퀀싱을 실시하였다. 손끝과 개인 소지품 간의 박테리아 매칭 비율의 정확성은 공식과 함께 환경 및 인간관련 데이터베이스를 사용하여 확인하였다. 적절한 분석을 설계하기 위해 다음 세가지 범주를 기준으로: 정성적 분석과 정량적 분석 비교, 성별에 관계없이 모든 참여자뿐만 아니라 동일 성별 참여자 내 비교, 환경(eDB) 및 인간 관련 데이터 베이스(hDB)를 이용하여 샘플간 비교하였다. 결과는 정성적 분석과 동일 성별 참가자 내에서의 비교 및 hDB의 사용이 비교적 정확한 결과를 제공하였다. 우리의 연구는 인간 유래 미생물을 사용하여 대량의 미생물학적 데이터를 포함하는 연구를 수행할 때 정확한 결과를 얻을 수 있는 분석 방법을 제공한다.

• 대한임상검사과학회지
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• 제50권3호
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• pp.331-336
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• 2018

본 연구에서는 국내 대전 소재 3차 병원 임상에서 분리된 Candida albicans 40 균주를 대상으로 균주 분리원에 따라 7종류의 housekeeping gene에 대한 염기서열 변이를 확인하고 MLST 분석을 통해 균주간의 계통학적 연관성에 대한 연구를 수행하여 다음과 같은 결과를 얻었다. Phylogenetic tree 분석 결과 sub-cluster 1로 central line blood (2), others (5), sputum (4), urine (7)을 포함해 총 18개가 분류되었으며, sub-cluster 2로는 central line blood (1), others (5), peripheral blood (6), sputum (1), urine (1)을 포함해 총 14개가 분류되어 동일 채취 부위에서 분리된 균주는 유전학적으로 유사성을 가지고 있을 가능성이 있음을 확인하였다. 앞으로 분리지역과 상병 등에 따른 C. albicans 유전자들의 변이 추세에 대한 자료의 축적과 임상 검체에 따른 계통학적 관계를 추정하여 감염병 연구 및 역학적 감시체계 구축에 도움이 될 것으로 생각된다.

• Journal of Ginseng Research
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• 제46권2호
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• pp.235-247
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• 2022

Background: Ginsenoside Rg3 is one of the main active ingredients in ginseng. Here, we aimed to confirm its protective effect on the heart function in transverse aortic coarctation (TAC)-induced heart failure mice and explore the potential molecular mechanisms involved. Methods: The effects of ginsenoside Rg3 on heart and mitochondrial function were investigated by treating TAC-induced heart failure in mice. The mechanism of ginsenoside Rg3 for improving heart and mitochondrial function in mice with heart failure was predicted through integrative analysis of the proteome and plasma metabolome. Glucose uptake and myocardial insulin sensitivity were evaluated using micro-positron emission tomography. The effect of ginsenoside Rg3 on myocardial insulin sensitivity was clarified by combining in vivo animal experiments and in vitro cell experiments. Results: Treatment of TAC-induced mouse models with ginsenoside Rg3 significantly improved heart function and protected mitochondrial structure and function. Fusion of metabolomics, proteomics, and targeted metabolomics data showed that Rg3 regulated the glycolysis process, and Rg3 not only regulated glucose uptake but also improve myocardial insulin resistance. The molecular mechanism of ginsenoside Rg3 regulation of glucose metabolism was determined by exploring the interaction pathways of AMPK, insulin resistance, and glucose metabolism. The effect of ginsenoside Rg3 on the promotion of glucose uptake in IR-H9c2 cells by AMPK activation was dependent on the insulin signaling pathway. Conclusions: Ginsenoside Rg3 modulates glucose metabolism and significantly ameliorates insulin resistance through activation of the AMPK pathway.

• 대한임상검사과학회지
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• 제43권1호
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• pp.19-25
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• 2011

The purpose of this study was to analyse brainstem auditory evoked potentials (BAEP) wave change data during microvascular decompression (MVD). The nerve function of Cranial Nerve VIII is at risk during MVD. Intraoperative monitoring of BAEP can be a useful tool to decrease the danger of hearing loss. Between January and December 2009, 242 patients had MVD for hemifacial spasm (HFS) and trigeminal neuralgia (TN). Among intraoperative BAEP changes, amplitude of V-V' was the most frequently observed during cerebellar retraction and decompression step of the MVD procedure. 138 patients (57%) had no BAEP change while 104 patients (42.98%) had BAEP change. 69 patients (28.5%) had Type A-I, 16 patients (6.6%) had Type A-II, 5 patients (2.1%) had Type B, and 13 patients (5.37%) had Type C. MVD is a surgical procedure to relieve the symptoms (e.g. pain, muscle twitching) caused by compression of a nerve by an artery or vein. During BAEP intraoperative monitoring, the surgical step is important in interpreting the changes of wave V. Several potential mechanisms of injury may affect the cochlear nerve, and complete loss of BAEP is often associated with postoperative hearing loss. Intraoperative BAEP monitoring may provide an early warning of hearing disturbance after MVD.

• 대한의생명과학회지
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• 제19권3호
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• pp.245-253
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• 2013

To provide reference data or guideline for empirical treatment of bloodstream infection, we studied a change pattern in causative microorganisms and antimicrobial susceptibility in a general hospital at Gyeonggi province during five years. We retrospectively reviewed the frequency of causative microorganisms and antimicrobial susceptibility results of 5,782 microorganisms isolated from blood culture in a general hospital during the period from January 2008 to December 2012. The most common pathogens were Escherichia coli (14.7%), Staphylococcus aureus (7.5%), Streptococcus viridans group (4.9%), and Klebsiella pneumoniae (4.1%). The multiple microorganisms were isolated in 4.3% of bloodstream infection patients. The average contamination rate of blood culture during five years was 3.0%. Methicillin-resistant S. aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), and penicillin-resistant Streptococcus pneumoniae were isolated at 62%, 27% and 11%, respectively. Cefotaxime-resistant E. coli and K. pneumoniae was 20% and 18%, respectively. Imipenem-resistant Pseudomonas aeruginosa (IRPA) and Acinetobacter baumannii (IRAB) was 25% and 66%, respectively. E. coli and S. aureus were most common pathogens isolated from blood culture for five years. The increase of multidrug-resistant microorganisms, such as MRSA, VRE, ESBL, IRPA and IRAB, requires more strict control of antibiotics and causes the need of the more updated guideline for the treatment of blood stream infection.

• 대한치과기공학회지
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• 제38권4호
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• pp.343-352
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• 2016

Purpose: The intention of the study is to reveal the factors that influence the safety-behavior and safety-accident of the students of dental laboratory science. We intend to use the study as a basic data of searching effective ways to heighten the safety-behavior of clinical training and to prevent safety-accident. Methods: The survey was conducted on dental technology students. The collected data was analyzed by the statistical program SPSS 21.0. The results were analyzed by reliability, frequency, t-test, correlation, multiple regression. To test for significance on each item, p<0.05 has been decided as a standard. Results: The results of the study showed that the safety of the students was influenced by the school year, the leader of clinical training, clinical training environment and the experience of safety education. The safety-accident turned out to be influenced by the school year of the student and the safety behavior. Conclusion: Active leader of clinical training, clinical training environment that enables the safety-behavior, and the offering of the systematic safety education were the most important factors to heighten the safety behavior of the students and prevent the safety-accident. These factors were expected to not only induce the safety-behavior but also prevent the safety-accident as well.