• Korean Journal of Clinical Laboratory Science
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• v.51 no.4
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• pp.414-419
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• 2019

JC polyomavirus (JCPyV) is a human pathogenic virus belonging to the family Polyomaviridae, a viral group containing dsDNA nucleic acid. A recent recommendation is to apply the presence of JCPyV as a fecal indicator for water contamination in environments like sewage, and techniques to monitor JCPyV in water are being proposed. To date, the conventional PCR system has been applied as a diagnostic method for detecting JCPyV. There is a need for a more rapid and sensitive JCPyV diagnostic detection method in clinical and environmental samples. In this study, we developed a loop-mediated isothermal amplification (LAMP) primer set for the detection of JCPyV. Our results indicate that the LAMP method using a specific primer set shows about 10-fold higher detection sensitivity than the conventional PCR system. The effectiveness of the LAMP method developed in this study has been validated by PCR product digestion using the HaeIII restriction enzyme. We, therefore, propose that the LAMP method using a specific primer set can be applied as a rapid and sensitive detection method for monitoring JCPyV in clinical and environmental samples.

• Clinical and Experimental Reproductive Medicine
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• v.20 no.1
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• pp.1-7
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• 1993

The present study was designed to test the validity of the semen analysis(S/A) and the sperm penetration assay(SPA) as a prognostic indicator of male fertility in 123 patients undergoing in vitro fertilization(IVF). We attempted to correlate the traditional semen parameters or the extent of sperm penetration in SPA with the results of human IVF rate or cleavage rate. Poor correlation was found between the results of S/A and human IVF rate(sensitivity, 80.6% ;specificity, 46.7%; positive predictive value, 91.6%;negative predictive value, 25%). Conversely, good correlation was found between the results of SPA and human IVF rate(sensitivity, 100% ; specificity, 80% ;positive predictive value, 97.3% ;negative predictive value, 100%). Our results corroborate the conclusion that SPA can be a valuable tool as a prognostic indicator of male fertilizing ability.

• The Journal of Korean Academic Society of Nursing Education
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• v.22 no.4
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• pp.577-587
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• 2016

Purpose: This study was conducted to provide Simulation Problem-Based Learning (S-PBL) for community visit nursing students according to their Myers Briggs Type Indicator (MBTI) personality types for learner-focused education and to verify its effectiveness. Methods: This study was conducted with subjects having the ST (Sensing-Thinking) and NF (iNtuition-Feeling) personality types, which are conflicting personality types, and forty-one subjects were allocated to the experimental group and nineteen subjects to the control group. The training, provided to subjects once a week, for a total of six times and 100 minutes each time, was with respect to the improvement of problem-solving ability, communication ability, and clinical practice competency. Data were analyzed through t-test and independent t-test. Results: After training, the self-rated problem-solving competency (t=3.07, p=.003), communication ability (t=2.86, p=.006), and academic self-efficacy (t=2.44, p=.018) were significantly higher in the experimental group than in the control group. However, there was not a significant difference in the clinical practice ability rated by subjects themselves (t=1.50, p=.140) and by professors (t=1.08, p=.285), and in the communication ability rated by professors (t=0.72, p=.474). Conclusion: The community visit nursing S-PBL, according to MBTI personality types, is a helpful learning method for nursing students participating in self-directed learning of nursing theory and practice.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8595-8601
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• 2014

Background: miR-200a expression is frequently altered in numerous cancers. The aim of the present study was to determine the role of microRNA-200a in advanced ovarian carcinomas. Materials and Methods: We measured miR-200a expression in 72 matched normal ovarian tissues and advanced ovarian carcinomas, and also two ovarian carcinoma cell lines (SKOV3 and SKOV3.ip1 - the latter being more invasive and metastatic than the parental SKOV3) by stem-loop real-time RT-PCR based on TaqMan microRNA assay using U6 as a reference. Levels of miR-200a expression were compared by disease stage, tumor grade, histology, and lymph node involvement. To evaluate the role of microRNA-200a, cell proliferation and invasion of SKOV-3 and SKOV-3.ip1 were analyzed with miR-200a inhibitor/mimic transfected cells. Results: Of 72 paired samples, 65 cancer tissues overexpressed microRNA-200a greater than two fold in comparison with matched normal epithelium. Specifically, patients with lymph node metastasis showed significant elevation. The level correlated with clinicopathological features, including high tumor grade, late disease stage, most notably with lymph node metastasis, but not with tumor histology. In addition, SKOV-3.ip1 cells also overexpressed miR-200a compared with SKOV-3, and miR-200a inhibitor transfected SKOV-3.ip1 cells showed significant reduction in cellular proliferation and invasion, while a miR-200a mimic stimulated the opposite behavior. Conclusions: We provide definitive evidence that miR-200a is up-regulated in a significant proportion of advanced ovarian carcinomas, and that elevated miR-200a expression facilitates tumor progression. Our findings support the notion that miR-200a is an onco-microRNA for ovarian cancer, and elevation is a useful potential diagnostic indicator. This study also provides a solid basis for further functional analysis of miR-200a in advanced ovarian cancer.

• 한국사회복지학회:학술대회논문집
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• 2004.04a
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• pp.757-779
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• 2004

The purpose of study is to provide efficient initial approaches for counseling in social work practice settings. First, it attempts to identify the correlation between the Myers-Brigg's Type Indicator(MBTI), a personality index based on the theory of psychological typology, and Personality Adaptation Types(PAT) models. Second, it attempts to develop a counseling method which can be applied to the initial interview, using the paradigm of door to therapy such as "Open Door", "Target Door", and "Trap Door" based on the PAT theory. The result of the quantitative study showed the correlation between MBTI and PAT as follows: MBTI Extrovert and Feeling(E, F) type was statistically correlated to hysterical PAT. MBTI Extrovert, Intuition and Perceiving(E, N, P) type was statistically correlated to Obsessive-Compulsive PAT. In conclusion, this study finds that when a clinical social worker approaches a client with the client's MBTI personality type and an interventional personality adaptation theory approach such as "Open Door", "Target Door", or "Trap Door", the efficiency of communication is increased significantly.

• The korean journal of orthodontics
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• v.23 no.4 s.43
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• pp.607-618
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• 1993

If the centric prematurity occurs after orthodontic treatment, it creates centric slide regarded as a possible factor in the cause of temporomandibular disorder and/or postorthodontic relapse. The purpose of this study was to investigate the manner of centric prematurity and centric slide in postorthodantic patients. The 36 orthodontic patients who had been treated with edgewise appliance at least 3 mouths previously were used in this study. After recording centric relation by the leaf gauge technique, the centric prematurity and centric slide were studied using SAM2 articulator and mandibular position indicator. The results were as follows : 1. The highest percentage of centric prematurities were found on the second molars. 2. The buccal incline of the palatal cusp was the most frequent area of centric prematurities in the maxilla, while the lingual incline of the buccal cusp was the most frequent area in the mandible. 3. There were no trends in the direction of centric slide on the mandibular position indicator. 4. There were no significant differences in centric discrepancies between the premolar extraction and nonextraction group.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.2
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• pp.346-353
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• 1995

The dietary requirement for zinc to maintain optimally the various metabolic and physiological funcitons is still under study. Human beings adapt to reductions in zinc intake by reducing the rate of growth or zinc excretion. Reductions in dietary zinc beyond the capacity to maintain homeostasis lead to utilization of zinc from an exchangeable pool. Loss of a small, critical amount of zinc from this pool leads to both biochemical and clinical signs of zinc deficiency. Zinc requirements have been assessed by balance studies and factorial method. As tissue zinc status influences endogenous losses and the dietary needs, individuls in good status may require higher amounts of zinc than those in poor status. While plasma zinc is insensitive to reducitons in dietary zinc, it is regarded as a valid, useful indicator of the exchangeable pool of zinc. Plasma metallothionein concentrations may prove useful for identifying poor zinc status. It has been suggested that functional end point measurement is the new direciton for zinc requirement. However, determination of the functional response to a marginal zinc intake is difficult because of the lack of a specific, sensitive indicator of zinc status. Presently, no good method for assessment of human zinc requirements exists.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.15
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• pp.6599-6603
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• 2015

Background: For the determination of creatine kinase (CK)-MB, the immunoinhibition method is utilized most commonly. However, the estimated CK-MB activity may be influenced by the presence of CK isoenzymes in some conditions like cancer. Thus, a CK-MB-to-total-CK ratio more than 1.0 could be found in such a situation. The study aimed to explore the relationship of cancer to high CK-MB-to-total-CK ratio. Materials and Methods: From January 2011 to December 2014, laboratory data on all CK-MB and total CK test requests were extracted at Far Eastern Memorial Hospital (88,415 requests). Patients with a CK-MB-to-total-CK ratio more than 1.0 were registered in this study. Clinical data including tumor location, tumor TNM stage and metastatic status were also collected. Results: A total of 846 patients were identified with a CK-MB-to-total-CK ratio more than 1.0. Of these, 339 (40.1%) were diagnosed with malignancies. The mean CK-MB-to-total-CK ratio was significantly higher in malignancy than in non-malignancy ($1.35{\pm}0.28$ vs $1.25{\pm}0.23$, p<0.001) groups. The most frequent malignancy with a CK-MB-to-total-CK ratio more than 1.0 was colorectal cancer ($1.42{\pm}0.28$, 16.5%, n=56), followed by lung cancer ($1.38{\pm}0.24$, 15.9%, n=54) and hepatocellular carcinoma (14.5%, n=49). Higher CK-MB-to-total-CK ratios in hematological malignancies ($1.44{\pm}0.41$)were also noted. Additionally, the CK-MB-to-total-CK ratio was markedly higher in advanced stage malignancy than in early stage ($1.37{\pm}0.26$ vs. $1.29{\pm}0.31$, p=0.014) and significantly higher in liver metastasis than in non-liver metastasis ($1.48{\pm}0.30$ vs. $1.30{\pm}0.21$, p<0.001). Conclusions: The CK-MB-to-total-CK ratio is an easily available indicator and could be clinically utilized as a primary screening tool for cancer. Higher ratio of CK-MB-to-total-CK was specifically associated with certain malignancies, like colorectal cancer, lung cancer and hepatocellular carcinoma, as well as some cancer-associated status factors such as advanced stage and liver metastasis.

• International Journal of Internet, Broadcasting and Communication
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• v.13 no.1
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• pp.107-115
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• 2021

This paper presents a smartphone application for the clinical practice education conducted in hospitals, with an aim to evaluate its effectiveness. A nonequivalent control group posttest design was used, which included a total of 100 nursing of school students who conducted their clinical practice. They were divided into one control and one experimental group (50 students each). The control group was directly trained in the clinical practice orientation, and the experimental group was a group who self-learned the clinical practice orientation using a smartphone application. Research data were collected between March 5 and April 27, 2019. They were analyzed with descriptive statistics and independent t-test, using the SPSS Statistics Version 24. The smartphone application customized for the clinical practice education was implemented through the following four phases: analysis, design, development, and evaluation. The developed application was registered in Google Play (for Android apps) and Apple Store, and related information was provided, making it available for download. The study showed that the satisfaction with and self-confidence in learning differed significantly between the groups. However, technology acceptance and knowledge acquired through practice showed no statistically significant difference. The research results serve as basic data for applying smartphone applications as an educational method that can replace traditional modes of education, serving as a significant indicator of the education delivery method diversification.

• Journal of Chest Surgery
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• v.28 no.1
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• pp.7-10
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• 1995

Mixed Venous oxygenation saturation[SvO2 is a variable determined in part by the externally controlled factors and in part by the patient during CPB. I monitored the SvO2 and tested it as a parameter for the regulation of pump output and as a criteria for the need of inotropics after CPB. With the help of SvO2, I increased the pump flow especially during rewarming for more optimal oxygenation of cells. After CPB, the calculated cardiac index was used as an indicator for the need of inotropic support with greater accuracy and without any clinical problems. I conclude that the SvO2 is an easily checkable variable and a good indicator for optimal oxygenation at cell level, and can be used as an objective criteria for the need of postoperative inotropic support.