• Journal of Veterinary Clinics
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• v.28 no.1
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• pp.139-143
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• 2011

A Spitz (5-years old, castrated male) and a Maltese (9-years old, castrated male) were presented with weight loss, polyuria/polydipsia (PU/PD) and depression. Diabetic ketosis was diagnosed based on clinical signs, physical examinations and screening tests (CBC, serum chemistry and urinalysis). The dogs were treated with NPH initially. However, NPH was inadequate to control blood glucose level due to the short duration of the action (< 5 hours). Because of the poor glycemic control with NPH, the dogs showed diabetic complications including weight loss and cataract. After change to glargine, the duration of insulin action was extended up to 11 hours. As a result, there was significant improvement in clinical signs and serum fructosamine concentrations. This study suggests that glargine is useful as a long-acting insulin in dogs which have poor glycemic control due to the short duration of NPH.

• Korean Journal of Veterinary Research
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• v.28 no.2
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• pp.307-310
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• 1988

This experiment was carried out to provide the data of blood chemistry in use of application to clinical diagnosis in racehorses. Blood was collected from 30 racehorses(♀ 17, ♂ 13). Calcium, inorganic phosphorus, blood glucose, blood urea nitrogen, cholesterol, bilirubin, fibrinogen and ketone body in serum were analyzed as follows. 1. The values of calcium and inorganic phosphorus were maintained a certain level without much variation regardless of sex and species, Ca/P ratio was in the range of 3.13~3.35. 2. The value of blood glucose was 60 mg/100ml, Age and sex of racehorses make no difference in the value. 3. The value of blood urea nitrogen and total cholesterol showed a tendency to increase according to the age. Total cholesterol value was somewhat higher in male than in female. 4. The value of total bilirubin showed a tendency to increase according to the age. There was no difference between sexes in the value. 5. The value of fibrinogen showed no apparent difference with age and sex. 6. Ketone body was negative in all of the cases. 7. There was no variation in the species of the racehorses.

• Journal of Veterinary Clinics
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• v.22 no.1
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• pp.70-73
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• 2005

A 9-year old female Shih Tzu dog was presented to the Veterinary Medical Teaching Hospital of Seoul National University with a history of chronic intermittent anorexia and vomiting of 4-year duration. She visited 3 years ago with the same clinical signs but has not been treated regularly due to waxing-waning course. On the physical examination, no specific findings were found. CBC, serum chemistry, radiography, ACTH stimulation test, evaluation of serum T4 and TSH concentration were performed. Hypoadrenocorticism was diagnosed with the ACTH stimulation test. And then, secondary hypoadrenocorticism was diagnosed with ACTH stimulation test, pre-ACTH aldosterone concentration, endogenous ACTH concentration. As electrolyte concentrations were normal, glucocorticoid (0.15 mg/kg bid PO) alone was administered. She has recovered from the clinical signs and has been doing well. It is suggested that the differentiation of secondary hypoadrenocorticism from primary hypoadrenocorticism is important as the secondary hypoadrenocorticism occurs rarely and the symptoms are non-specific with normal electrolyte concentrations.

• Journal of Veterinary Clinics
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• v.23 no.2
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• pp.202-206
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• 2006

A 7-year-old, spayed female, Yorkshire terrier dog with polyuria/polydipsia (PU/PD), interdigital edema, pruritus and abdominal enlargement was referred to the Veterinary Medical Teaching Hospital of the present university. Pituitary-dependent hyperadrenocortisim (PDH) was diagnosed by clinical signs, physical examination, laboratory examination (complete blood count, serum chemistry, urinalysis, ACTH stimulation test and high dose dexamethasone suppression test) and diagnostic imaging (radiography, ultrasonography and computed tomography). Clinical signs were improved after trilostane treatment, and maintenance therapy with trilostane still continued after successful induction therapy. Trilostane can be used as an alternative to mitotane therapy in hyperadrenocortisim in dogs.

• Korean Journal of Clinical Laboratory Science
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• v.36 no.2
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• pp.98-109
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• 2004

The lower limit of detection, precision, setting method of target value, reportable range determination, recovery, linearity, and comparison study with another equipment was evaluated for the Toshiba-120FR chemistry autoanalyzer which was newly introduced at the Daejeon Veteran Hospital in Dec. 2003. Nineteen kinds of test for AST, ALT, ALP, LDH, GGT, TP, ALB, GLU, T-cho, T-bil, TG, UA, CAL, IP, AMY, HDL-C, LDL-C, Cre and BUN were performed to evaluate the lower limit of detection, precision, setting method of target value, reportable range determination, recovery, linearity, and comparison study with other equipment according to the NCCLS guidelines(EP5-A, EP6-P, EP9-A). The Toshiba-120FR autochemical analyzer showed good precision for all tested items. The data concerning the lower limit of detection, precision(total CV 0.47%~3.65%), setting method of target value, reportable range determination, recovery(93%~111%), linearity($R^2=0.997{\sim}0.999$), and comparison study(r=0.977~0.999) with other equipment was acceptable for all tested items. The results of evaluation for the Toshiba-120FR autochemical analyzer showed that this equipment could be used as an alternative to other equipment.

• Korean Journal of Clinical Pharmacy
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• v.9 no.2
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• pp.119-126
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• 1999

The educational backgrounds and research interests of pharmacy educators in Korea were analyzed to investigate the possibility of migrating the pharmacy education system from basic science-oriented to pharmacy practice-oriented. There are 257 full-time faculty members and 190 part-time members in twenty pharmacy colleges in 1999. Most of the full-time professors are majoring in basic pharmacy areas focused on the drug itself. Thirty nine percent of full-time professors are specialized in the fields of Medicinal Chemistry, $20\%$ in Biochemistry, $19\%$ in Pharmaceutics, and $18\%$ in Pharmacology-Toxicology. Only $4\%$ are related to the Pharmacy Practice Field. When we consider only the educational backgrounds of the educators, $89\%$ of full-time professors are related to the subjects of Korean Pharmacists Licensure Examination. Changes in educator's inclination will lead to the changes in pharmacy education and the contents of the test. There will be at least 38 job-openings due to retirements within next five years, and this will lead to approximately $15\%$ changes of current full time professors in Korean pharmacy education. More full-time professors will be needed in the area of pharmacy practice in order to focus on the pharmacy practice-oriented pharmacy education. Many distinguished characteristics of pharmacy educators between the private schools and the national universities are also discussed in the paper.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.371-381
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• 2015

The object of this study was to obtain single oral dose toxicity of Arisaema Rhizome (Arisaema amurense f. serratum (Nakai) Kitag) aqueous extracts. Arisaema Rhizome (Chunnamsong in Korean) is one of the most important folk remedy plants used in Asia. In the study, a 28-day rat oral gavage study has been conducted with the extracts from Arisaema Rhizome at dose of 1,250, 2,500 and 5,000 ㎎/㎏/day. The following endpoints were evaluated: clinical observations, body weight, gross and microscopic pathology, clinical chemistry, and hematology. Based on the analysis of these endpoints, it was estimated that NOEL (no observed effect level) for male rats and NOAEL (no observed adverse effect level) for female rats are 5000 ㎎/㎏/day of the water-extracts from Arisaema Rhizome.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.14-14
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• 2020

In December 2019, the COVID-19 epidemic was discovered in Wuhan, China, and since has disseminated around the world impacting human health for millions. Herein, in-silico drug discovery approaches were utilized to identify potential candidates as Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) main protease (M^pro) inhibitors. We investigated several databases including natural and natural-like products (>100,000 molecules), DrugBank database (10,036 drugs), major metabolites isolated from daily used spices (32 molecules), and current clinical drug candidates for the treatment of COVID-19 (18 drugs). All tested compounds were prepared and screened using molecular docking techniques. Based on the calculated docking scores, the top ones from each project under investigation were selected and subjected to molecular dynamics (MD) simulations followed by molecular mechanics-generalized Born surface area (MM-GBSA) binding energy calculations. Combined long MD simulations and MM-GBSA calculations revealed the potent compounds with prospective binding affinities against M^pro. Structural and energetic analyses over the simulated time demonstrated the high stabilities of the selected compounds. Our results showed that 4-bis([1,3]dioxolo)pyran-5-carboxamide derivatives (natural and natural-like products database), DB02388 and Cobicistat (DB09065) (DrugBank database), salvianolic acid A (spices secondary metabolites) and TMC-310911 (clinical-trial drugs database) exhibited high binding affinities with SARS-CoV-2 M^pro. In conclusion, these compounds are up-and-coming anti-COVID-19 drug candidates that warrant further detailed in vitro and in vivo experimental estimations.

• Clinical and Experimental Vaccine Research
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• v.12 no.3
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• pp.193-208
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• 2023

One of the most significant medical advancements in human history is the development of vaccines. Progress in vaccine development has always been greatly influenced by scientific human innovation. The main objective of vaccine development would be to acquire sufficient evidence of vaccine effectiveness, immunogenicity, safety, and/or quality to support requests for marketing approval. Vaccines are biological products that enhance the body's defenses against infectious diseases. From the first smallpox vaccine to the latest notable coronavirus disease 2019 nasal vaccine, India has come a long way. The development of numerous vaccines, driven by scientific innovation and advancement, combined with researcher's knowledge, has helped to reduce the global burden of disease and mortality rates. The Drugs and Cosmetics Rules of 1945 and the New Drugs and Clinical Trials Rules of 2019 specify the requirements and guidelines for CMC (chemistry, manufacturing, and controls) for all manufactured and imported vaccines, including those against coronavirus infections. This article provides an overview of the regulation pertaining to the development process, registration, and approval procedures for vaccines, particularly in India, along with their brief history.

• Journal of Life Science
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• v.22 no.11
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• pp.1507-1514
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• 2012

Oxidative stress leads to damage in all components of the cell, including proteins, lipids, and DNA due to imbalance between reactive oxygen species production and cellular detoxification. Phytochemicals are well-known to contain antioxidants, and their physiological role has been intensively studied. The fruit of Cornus officianalis has been used in oriental medicine and has been reported to have many functions. In this study, four different extraction techniques were applied to extract functional components from the fruit of Cornus officianalis, and the content of loganin, which is an antioxidant having DPPH radical and hydrogen peroxide scavenging activity and reducing power, was analyzed in each extract. Extraction techniques employed in this study were heat extraction by water, 70% ethanol extraction, enzyme treatment, and combination of enzyme treatment and heat extraction by water. All extracts contained 11.8-18.0 mg/g loganin and showed antioxidation function assayed by measuring DPPH radical and hydrogen peroxide scavenging activity and reducing power. Among them, heat extraction was the most effective technique, showing a maximum amount of loganin (18.0 mg/g) and antioxidative activity at 100 mg/ml concentration. Each extract showed very low cytotoxicity up to at 500 mg/ml but 10-20% cytotoxicity at 1,000 mg/ml by in vitro MTT assay.