• 분석과학
• /
• 제22권6호
• /
• pp.498-503
• /
• 2009

폐암은 전 세계적으로 높은 사망률 때문에 죽음에 이르게 되는 암이다. 폐암은 소세포암과 비소 세포암 두 종류로 나누어 지는데 비소세포암 환자에게서 EGFR 돌연변이가 발견되었고 이 EGFR 돌연변이는 방사선치료와 EGFR tyrosin kinase의 저해제인 gefitinib과 erlotinib의 반응에도 연관되어진다. 이와 같이 EGFR 돌연변이의 검출은 효과적인 치료방법을 제시해 줄 수 있을 것이다. 본 연구에서는 간단하고 값이 싸며, 반응시간이 더 빠르게 유전자 다형성을 검출 할 수 있는 칩-기반 등온증폭반응을 수행하였다. 그 결과, 이 등온증폭 방법은 빠른 증폭 시간과 높은 민감성, 특이성을 가지고 있었으며 현증 검증에서 EGFR 돌연변이를 빠르고 정확하게 진단할 수 있는 유용한 방법이 될 것이다.

• 대한임상검사과학회지
• /
• 제37권1호
• /
• pp.1-7
• /
• 2005

The purpose of this study was to verify (i) a consistent calibration verification for the assessment of method linearity and (ii) calibration agreement with calibration settings. We validated calibration verification through method linearity with different lot number of individual calibrators that span the working range for 9 tests except salicylate with control sample in test. We evaluated that it covered broad analyte range to assay from near zero to the top of the measuring range with 5 or 6 points every three times for 10 analytes in TDM test. Target values were plotted on X-axis with assigned or observed values on the Y-axis. Working range were as follows. Calibration verification of the measuring range (maximum to minimum values) has been validated asetaminophen 0.1 to $304.6_{\mu}g/mL$, salicylate 0 to $1005_{\mu}g/mL$, valproic acid 3.2 to $154.19_{\mu}g/mL$, digoxin 0.17 to 5.65 ng/mL, vancomycine 1.3 to $80.51_{\mu}g/mL$, carbarmazepine 0.1 to $22.3_{\mu}g/mL$, phenytonin 0.6 to $40.21_{\mu}g/mL$, theophyline 0.2 to $40.21_{\mu}g/mL$, primidone 0 to $24.07_{\mu}g/mL$, phenobarbital 0.6 to $60.0_{\mu}g/mL$. Drawing a straight line through five or six points of these data showed good linearity. We are sure that it is important to assess the calibration verification of a test method to ascertain the lowest and highest test results that are reliable.

• 대한임상검사과학회지
• /
• 제38권2호
• /
• pp.117-124
• /
• 2006

The analytical measurement range (AMR) is the range of analyte values that a method can directly measure on a specimen without any dilution, concentration, or other pretreatment not part of the usual assay process. The linearity of the AMR is its ability to obtain test results which are directly proportional to the concentration of analyte in the sample from the upper and lower limit of the AMR. The AMR validation is the process of confirming that the assay system will correctly recover the concentration or activity of the analyte over the AMR. The test specimen must have analyte values which, at a minimum, are near the low, midpoint, and high values of the AMR. The AMR must be revalidated at least every six months, at changes in major system components, and when a complete change in reagents for a procesure is introduced; unless the laboratory can demonstrate that changing the reagent lot number does not affect the range used to report patient test results. The AMR linearity was total protein (0-16.6), albumin (0-8.1), total bilirubin (0-18.1), alkaline phosphatase (0-1244.3), aspartate aminotransferase (0-1527.9), alanine aminotransferase (0-1107.9), gamma glutamyl transpeptidase (0-1527.7), creatine kinase (0-1666.6), lactate dehydrogenase (0-1342), high density lipoprotein cholesterol (0.3-154.3), sodium (35.4-309), creatinine (0-19.2), blood urea nitrogen (0.5-206.2), uric acid (0-23.9), total cholesterol (-0.3-510), triglycerides (0.7-539.6), glucose (0-672.7), amylase (0-1595.3), calcium (0-23.9), inorganic phosphorus (0.03-17.0), potassium (0.1-116.5), chloride (3.3-278.7). We are sure that materials for the AMR affect the evaluation of the upper limit of the AMR in the process system.

• 대한임상검사과학회지
• /
• 제47권4호
• /
• pp.188-193
• /
• 2015

경동맥 내중막 두께는 뇌혈관질환과 관상동맥질환을 정밀하게 측정하는 검사이다. 많은 선행논문에 따르면 경동맥 내중막 두께는 죽상동맥경화를 예측하고 심혈관질환의 위험인자와 연관성이 높으며, 심근경색이나 뇌졸중 발생 위험을 예측할 수 있는 독립인자로 연구되었다. 따라서 본 연구는 혈액검사 중 임상화학적 검사결과와의 상관관계 연구를 통해 경동맥 내중막 두께와 다른 독립인자를 확보하는 것이 목적이었다. 연구결과 피험자의 부족으로 인해 심혈관계관련 임상화학적 요인과는 상관관계를 입증하지 못했으나, 경동맥 내중막 두께와 ALT (p<0.05), GGT (p<0.05), Uric acid (p<0.05), CEA (p<0.05)와 양의 상관관계를 통계적인 유의수준으로 입증하여 지속적인 연구의 문제를 제기하였다.

• Bulletin of the Korean Chemical Society
• /
• 제29권3호
• /
• pp.666-668
• /
• 2008

• Bulletin of the Korean Chemical Society
• /
• 제24권7호
• /
• pp.911-915
• /
• 2003

Two liquid chromatographic chiral stationary phases (CSPs) based on (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid were successfully applied in the resolution of racemic tocainide and its analogues. In the resolution of tocainide, especially, the CSP containing N-CH₃ amide tethering groups was quite effective, showing clear baseline resolution (RS: 2.66) with reasonable enantioselectivity ( a: 1.25). Consequently, the CSP containing N-CH₃ amide tethering groups is expected to be useful to monitor the enantiomeric composition of tocainide in clinical samples. In addition, the chromatographic behaviors for the resolution of tocainide and its analogues on the two CSPs were found controllable by varying the content and the type of organic and acidic modifiers in aqueous mobile phase.

• 한국임상수의학회지
• /
• 제17권1호
• /
• pp.219-224
• /
• 2000

This study was conducted to examine the prognosis of canine pyometra after surgical treatment in terms of their clinical indications, hematology and serum chemistry. A total of 20 bitches diagnosed as pyometra after physical examination was hospitalized at the Veterinary Medical Teaching Hospital, Seoul National University, during the period of 1998 to 1999. After examining all profiles, ovariohysterectomy was performed to treat pyometra. All bitches had clinical symptoms of general depression, polyuria/polydipsia, anorexia and abdominal distension. In hematologic profiles, 65% of bitches had higher level of leukocyte count compared with normal level, but RBC and PCV values maintained within the normal ranges in most pyometric bitches. In serum chemistry profiler, ALT and ALP values were increased over the normal range, and BUN and creatine values were higher than normal ranges only in 10(50%) and 3 bitches (15%), respectively. Survival rate after ovariohisterectomy was 90% (18/20), but bitches that had higher ALT, ALP, BUN, creatine and potassium than normal values were died after surgical treatment. The results of this clinical investigation show that the recovery of pyometric bitches after ovariohysterectomy can be prognosed by hematologic and serum profiles including ALT, ALP, BUN, creatinine and potassium.

• 대한본초학회지
• /
• 제25권3호
• /
• pp.43-51
• /
• 2010

Objectives : We investigated the repeated-dose toxicity of Wenpitang-Hab-Wulingsan(WHW), a Korean traditional medicine prescribed with twelve herbs, which has been used for the treatment of renal disease. Methods : WHW extract prepared by GLP company. WHW was supplemented by gavage at 0, 100, 500 and 1000 mg/kg/day for 13-week consecutive days. We recorded the clinical signs of toxicity, body weight, organ weights, hematology, gross and histological changes in target organs rats and clinical chemistry analysis for all rats. Results : WHW extract at all doses was shown no mortality or abnormal clinical signs in rats during at the observation period. Furthermore, there was no difference in body weight and food-take consumption, organ weight, gross pathological findings, and urine analysis among the groups of rats treated with different doses of WHW extract. The hematological analysis and clinical blood chemistry data were revealed no toxic effects from WHW-treated rats. Conclusions : The results suggest that WHW extract in rats is a wide margin of safety on a acute toxicity.

• Bulletin of the Korean Chemical Society
• /
• 제31권9호
• /
• pp.2561-2564
• /
• 2010

The accurate determination of bisphosphonate levels in bone and biological fluids is important in both clinical and pharmacological/toxicological studies; however, the quantitative analysis of the bisphosphonate is difficult because its concentration is quite low in most of biological sample. A novel fluorescent chemosensor (FCS)-based measurement method of bisphosphaonate levels using Naphta-diDPA-$2Zn^{2+}$ (NadDPA-$2Zn^{2+}$, DPA = dipycolylamine), an excellent FCS previously used for detecting PPi, was developed. By the FCS method, the concentration of bisphosphonates having no fluorophores can be determined analyzed with sufficient sensitivity. The results of this study indicate that the FCS-based measurement can be a useful method to analyze bisphosphonates in biological samples.

• 공업화학
• /
• 제32권3호
• /
• pp.253-259
• /
• 2021

Colorectal cancer (CRC) is one of the most prevalent diseases in modern society, constituting a serious threat to global health. Currently, routine clinical screening and early removal of precancerous polyps are the most successful methods for reducing CRC incidence and mortality. However, the high cost and invasive detection of sigmoidoscopy and colonoscopy limited the CRC-screening participation and prevention. The emergence of biosensors provides an inexpensive, sensitive, less invasive tool for detecting CRC disease biomarkers. This review highlights some of recent efforts made on developing biosensors with electrochemical and optical techniques targeting CRC specific protein biomarkers for early diagnosis and prognosis, potential applications, and future perspectives.