• 약학회지
• /
• 제2권1_2호
• /
• pp.15-17
• /
• 1953

For the discernment between Clematis brachyura, Maxim. and Clematis mandshurica, Rupr., the color reaction, Mg-HCl (Hg) reaction, is applied to the flavons contained in leaves of both plants. The positive one against the reaction in Clematis brachyura, Maxim and the negative one is Clematis mandshurica, Rupr.

• 사상체질의학회지
• /
• 제10권2호
• /
• pp.151-162
• /
• 1998

1. 목적(目的) 및 방법(方法) 음양오행적(陰陽五行的) 기미론(氣味論)에 입각(立脚)한 기존약물(旣存藥物)들을 사상의학내(四象醫學內)의 각 체질병증(體質病證)에 사용코자 했을때 아직까지도 그 약물(藥物)의 정확한 체질소속(體質所屬)과 사상의학적(四象醫學的) 약리(藥理)를 설명하지 못하고 혼돈(混沌)과 논쟁(論爭)에 빠져 있는 경우가 많았다. 이에 본 연구(硏究)는 위령선(威靈仙)이라는 약물(藥物)을 통해 약물(藥物)의 체질소속(體質所屬)과 체질병증하(體質病證下)에서의 용약정신(用藥精神) 그리고 체질약물(體質藥物)의 공통속성(共通屬性)을 객관화(客觀化)하며 각 다른 약물(藥物)의 약리규명(藥理糾明)의 객관화(客觀化) 연구(硏究)에 그 실마리를 찾고자 한다. 문헌적(文獻的) 고찰(考察)의 방법(方法)을 위주로 현재 사용되는 위령선(威靈仙)의 종류(種類)와 학명(學名)을 살펴보고 각 문헌(文獻) 상(上)에서 기술된 위령선(威靈仙)의 약효(藥效)를 검토하였고 필요에 따라 다른 약물(藥物)을 인용(引用)하였으며 동의보감(東醫寶鑑)과 사상처방(四象處方) 중에 위령선(威靈仙)을 단방(單方)으로 사용하거나 포함한 방제(方劑)가 있는 문(門)을 종합 정리하여 그를 통해 위령선(威靈仙)의 체질소속(體質所屬)과 효능(效能)을 비교 검토하였다. 2. 결과(結果) 및 결론(結論) 기존 본초론적(本草論的)으로 위령선(威靈仙)은 기미(氣味)가 고신온(苦辛溫)하여 색이 검고 약용부위(藥用部位)가 뿌리여서 상초인 폐(肺)를 겸(兼)하여 하강(下降)하며 조습거담(燥濕祛痰) 및 분별청탁(分別淸濁)하여 후박(厚朴)과 같이 조열(燥熱)이 응결(凝結)한 질환(疾患)에도 사용할 수가 있다. 이런 약리(藥理)는 단미(單味)를 떠나 사상의학내(四象醫學內)의 체질병증(體質病證)에 소속되는 과정중에서 태음인(太陰人)의 기액(氣液)의 분별청탁(分別淸濁)작용을 통한 폐이호(肺以呼), 간이흡(肝以吸)의 승강조절(昇降調節)의 용약정신(用藥精神)을 찾을 수 있고 성폐지진기(醒肺之眞氣)의 약성(藥性)을 가짐을 알수 있다. 기능(機能)과 구조(構造)를 동시에 개선(改善)시키명 단순 치습(治濕)이 아닌 기액지기(氣液之氣)의 호산흡취(呼散吸聚)관계로만 설명가능함을 또한 확인 할 수 있었다. 향후 사상체질(四象體質) 약물의 객관화(客觀化)에 문헌적(文獻的) 고찰(考察)의 방법은 그 유용성 및 가치가 크다 할수 있다.

• 대한한의학회지
• /
• 제27권4호
• /
• pp.182-190
• /
• 2006

Objective : Based on immunological mechanisms, this study examined whether subcutaneous (s.c.) injection of Clematis mandshurica Maxim. water extract (CMA) has anti-inflammatory effects, and its effect on $TNF-{\alpha}$, IL-1 and IL-10 release from synoviocytes on adjuvant arthritis (AA) in the rat. Methods : Complete Freund's adjuvant was used to induce AA in rats. Synoviocytes were separated by the method of collagenase and DNase digestion Synoviocytes proliferation was assayed by 3-(4, 5 dimethylthiazol 2 yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. $TNF-{\alpha}$, IL-1 and interleukin-10 (IL-10) production of synoviocytes was measured with ELISA. The expression of IL-10 mRNA of synoviocytes was determined using RT PCR. Results : There were significant secondary inflammatory reactions in AA rats, accompanied by the decrease of body and immune organs weight simultaneously. Synoviocytes proliferation of AA rats significantly increased, and the levels of $TNF-{\alpha}$ and IL-1 in supernatants of synoviocytes in AA rats were also elevated compared with the sham group. The administration of CMA (2, 5, 10 mg/kg, s.c.) reduced the above changes significantly. In contrast to $TNF-{\alpha}$ and IL-1, IL-10 production and the level of its mRNA of synoviocytes in AA rats apparently decreased. CMA (2, 5, 10 mg/kg, s.c.) markedly increased IL-10 in synoviocytes at protein and transcription level. Conclusion : The results indicate that CMA has a beneficial effect on rat AA due to modulating inflammatory cytokine production of synoviocytes, which play a crucial role in the pathogenesis of this disease.

• 대한한의학회지
• /
• 제27권4호
• /
• pp.174-181
• /
• 2006

Objective : Based on the immunological dysfunction in RA and the immunological feature of Clematis mandshurica Maxim. herbal acupuncture solution (CM-HAS), this study examined whether subcutaneous (s.c.) administration of CM-HAS has anti-inflammatory effects on adjuvant arthritis (AA) in the rat. Methods : Complete Freund's adjuvant was used to induce AA in rats. Secondary paw swelling of AA rats was measured, and polyarthritis index was scored. The administration of CM-HAS (2, 5, 10 mg/kg) to the Sinsu-hyul (BL 23) acupuncture point subcutaneously (s.c.) inhibited the inflammatory response and restored the weight of body and immune organs of AA rats. In this study. inhibitory effect of CM-HAS on cyclooxygenase-2 (COX-2) was evaluated. The plant material selected for this study has been used in Korean medicine for the treatment of various diseases that are considered as inflammatory in nature, e.g. asthma, arthritis, rheumatism, fever, edema, infections, snakebite and related inflammatory diseases. Results : There were significant secondary inflammatory reactions in AA rats, accompanied with the simultaneous decrease of body and immune organs weight. The administration of CM-HAS (2, 5, 10 mg/kg, s.c.) improved the above changes significantly. CM-HAS showed inhibitory activity against COX-2, which supports their traditional uses. In this study, aspirin and indomethacin were used to contrast with CM-HAS as the COX-2 inhibitors. Conclusion: The findings of this study may explain at least in part why CM-HAS has been traditionally used for the treatment of inflammatory conditions in traditional Korean and Chinese medicine.