• Advances in Traditional Medicine
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• v.10 no.3
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• pp.214-221
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• 2010

Clematis chinensis is the root of Clematis chinensis OSBECK and is classified in Ranunculaceae. Clematis chinensis is a traditional medicinal herb possesses analgesic, diuretic, anti-tumorigenic, and anti-inflammatory effects. In this study, the effect of aqueous extract of Clematis chinensis against lipopolysaccharide-induced inflammation was investigated in mouse BV2 microglial cells. The aqueous extract of Clematis chinensis at the respective concentration was treated one hour before the lipopolysaccharide treatment in mouse BV2 microglial cells. From the present results, pre-treatment with the aqueous extract of Clematis chinensis suppressed prostaglandin E2 synthesis and nitric oxide production by inhibiting on the lipopolysaccharide-stimulated cyclooxygenase-2 and inducible nitric oxide synthase expressions in mouse BV2 microglial cells. The present study suggests that Clematis chinensis may offer a valuable mean of therapy for brain inflammatory diseases.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.1
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• pp.123-128
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• 2004

Numerous novel ingredients have been introduced for the higher functionality of whitening cosmetics. Through the preliminary research, we have found Trichosanthes kirilowii root, Prunella vulgaris leaf and Clematis chinensis root have high whitening efficacy. But they are insoluble. Moreover the discoloration of and decrease in content take place when they are exposed to light, heat or oxygen. From Trichosanthes kirilowii root, Prunella vulgaris leaf and Clematis chinensis root, efficacious ingredients were ethanol-extracted by heating to 75∼85$^{\circ}C$ for 6∼8 h. These extracts have the inhibitory activity of tyrosinase and B16 melanin formation, thus enhancing whitening effect. We made liposomes using propylene glycol (PG)/hydrogenated lecithin/middle chain triglycerides (MCT)/glycerin/water and microfuidizer to stabilize extracts. The stability against heat and light was enhanced by 3∼5 times compared with untreated extracts. Particle size analyzer, freeze fracture transmission electron microscopy (FF-TEM), chromameter and HPLC are used for the analysis.

• Journal of Life Science
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• v.34 no.6
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• pp.393-398
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• 2024

The purpose of this study was to verify the antifungal properties of various herbal medicines belonging to the Ranunculaceae family and to find an extraction method effective in inhibiting fungal growth. When antifungal activity was measured in a liquid medium with extracts obtained by either hot water extraction or organic solvent extraction of the herbal medicines Clematis apiifolia, Coptis chinensis, and Pusatilla chinensis, effective results were obtained from the chloroform extract. In addition, fungal growth inhibition experiments were performed on unicellular fungi, Candida albicans, Candida tropicalis, and Candida lusitaniae, and on filamentous fungi, such as Pythium ultimum, Aspergillus fumigatus, and Fusarium oxysporum, using disk diffusion experiments on solid media. It was confirmed that P. chinensis extract has excellent antifungal properties against Candida spp. and C. apiifolia extract against filamentous mold. Finally, GC-MS analysis was performed to explore the useful antifungal substances present in the extract. As a result of the study, thurbergenone from C. apiifolia and 16-hydroxycleroda-3, 13(14)-dien-15, 16-olide (16-HCDO) from C. chinensis were confirmed as antifungal candidates. In conclusion, it was confirmed that C. apiifolia, C. chinensis, and P. chinensis have antifungal activity against various fungi, and in GC-MS analysis, all herbal medicines were confirmed to have different antifungal candidates. These results indicate that the Ranunculaceae family has evolved in several directions for fungal resistance traits.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.6
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• pp.69-79
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• 2005

This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

• Korean Journal of Veterinary Service
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• v.35 no.1
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• pp.25-31
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• 2012

This study examined the efficacy of natural ingredient formulated herbal medicine as feed additives against coccidiosis in broiler chicken. Medicinal herbs used in this study were Artemisia annua, Pulsatilla chinensis, Clematis chinensis and Scutellaria baicalensis. Two week old chicks were administered with 1% ingredient formulated herbal medicine. Observations of the efficacy were confirmed by examining the lesion score, histopathological appearance of cecum, shedding of fecal oocyst and production index in farm. S. baicalensis of the most effective substance were analyzed by UPLC. The results are as follows; among various types of Chinese medicine, the most efficacious ingredient was found to be pure bicalein. The major index substance is bicalein and $13{\mu}g/ml$ per gram was found. Feeding of chickens with S. baicalensis induced the coccidiostat effect and reduced the fecal shedding of E. tenella oocyst by 14%. Experimental results on a farm, feed conversion ratio and weight gain and production rate in the group with natural ingredient formulated additives than in control group increased. The rate of weight gain (%) in each experimental group showed 108% and 126% increase while the mortality rate was 1.4% lower than the control group. The investigation showed that the most efficacious ingredient was found to be S. baicalensis among herbal medicine products for coccidiostats. However, as it was found in the farm experiment that pure S. baicalensis alone is economically unviable. The additives with cocktailed of efficacious ingredient are suggested to be implemented for effective protection against coccidiosis.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.6
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• pp.41-61
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• 2016

This study was carried out to offer the basic data for establishing the management plan and constructing the ecological data by surveying and analysing the flora distributed in main survey sites(Mt. Gwangdeok, Mt. Cheonggye and Mt. Jukyeop), Hanbuk-Jeongmaek. The flora were summarized, numbering 659 taxa including 99 families, 341 genera, 575 species, 4 subspecies, 69 varieties and 11 forms. The rare plants were 16 taxa including Tylophora floribunda, Hanabusaya asiatica, Lilium cernuum, Belamcanda chinensis and so forth. The Korean endemic plants were 24 taxa including Salix koriyanagi, Aconitum pseudolaeve, Clematis trichotoma, Hosta minor and so forth. The specific plants by floristic region were 95 taxa including 32 taxa of grade I, 31 taxa of grade II, 25 taxa of grade III, 5 taxa of grade IV and 2 taxa of grade V. The naturalized plants were 38 taxa including Fallopia dumetorum, Silene armeria, Cosmos bipinnatus, Poa pratensis and so forth. The invasive alien plants were 4 taxa including Ambrosia artemisiifolia, Ambrosia trifida, Aster pilosus and Eupatorium rugosum.

• The Korea Journal of Herbology
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• v.38 no.5
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• pp.39-47
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• 2023

Objectives : The objective of present study was to investigate the vasorelaxant effects of 20 Korean native plants on isolated rat thoracic aorta precontracted with phenylephrine (PE). Methods : Dried 20 plant materials were extracted 3 times with water, ethanol, or methanol for 3h in the reflux apparatus at 70 ± 5℃. Male SD rats were anesthetized by ether inhalation, and their aorta rings were isolated and placed in 10 ㎖ Krebs Henseleit (KH) buffer. While using an isolated organ-chamber technique, the aorta rings were maintained by bubbling with a gas mixture of 95% O₂-5% CO₂ at 37℃. Changes in isometric tension of aorta rings were recorded via isometric transducers connected to a Powerlab Data Acquisition System. Results : Among the 20 native plants, Chrysanthemum indicum L. flower, Nelumbo nucifera Gaertn. rhizome, Schisandra chinensis (Turcz.) Baill. fruit, Anthriscus sylvestris (L.) Hoffm. root, Corydalis turtschaninovii Besser tuber, Corydalis decumbens (Thunb.) Pers. tuber, and Dolichos lablab L. seed showed significant vasorelaxant effect on the contraction of aorta rings induced by PE. In contrast, Mertensia maritima subsp. asiatica Takeda whole plant, Ajuga decumbens Thunb. whole plant, Trigonotis peduncularis (Trevis.) Benth. ex Baker & S.Moore whole plant, Dioscorea quinquelobate Thunb. rhizome, Allium microdictyon Prokh aerial part, Momordica charantia L. fruit, Carthamus tinctorius L. flower, and Clematis terniflora DC. root constricted more the aorta rings precontracted by PE Conclusion : These results suggest that the possibility as useful herbal resources for the development of functional foods or medicines for hypertension treatment.

• Journal of Environmental Impact Assessment
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• v.28 no.3
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• pp.231-244
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• 2019

The purpose of this study is to offer the basic information for the rational management plan in construction and running of eco-forest by analyzing the vascular plant of the constructreserved site of eco-forest in Mt. Daeun, Yangsan-si, Gyeongsangnam-do. The vascular plants were 280 taxa; 75 families, 179 genera, 245 species, 3 subspecies, 28 varieties and 4 forma. The Korean endemic plants were 6 taxa including Pseudostellaria coreana, Stewartia pseudocamellia, Primula modesta var. hannasanensis and so forth. The rare plants were Juniperus chinensis var.sargentii, Prunus yedoensis, Primula modesta var. hannasanensis, Chionanthus retusus and Scopolia japonica. The naturalized plants were 14 taxa including Rumex acetosella, Lepidium apetalum, Trifolium repens, Oenothera biennis, Veronica persica and so forth. The invasive alien plants were Rumex acetosella and Ambrosia artemisiifolia. Specific plant species by floristic region were total 24 taxa; Prunus yedoensis and Primula modesta var. hannasanensis in class V, Juniperus chinensis var. sargentii and Acer pictum subsp. mono in class IV, 5 taxa including Dryopteris bissentiana, Scolopolia japonica and so forth in class III, Pseudostellaria coreana, Potentilla dickinsii and Chionanthus retusus in class II, 12 taxa including Acotinum jaluense, Clematis patens and so forth in class I.

• Journal of Environmental Science International
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• v.23 no.5
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• pp.911-942
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• 2014

This study was carried out to investigate the flora of indigenous habitat of Donghae-si and Samcheok-si in Gangwon-do, Korea. The vascular plants were collected 18 times (from March to October 2011), and were identified as 804 taxa; 127 families, 445 genera, 720 species, 8 subspecies, 69 varieties, 5 forms and 2 hybrids. In the flora of this area, the calcicolous plants were 55 taxa; Cheilanthes argentea, Clematis serratifoliam, Quercus variabilis. Epipactis papillosa, etc. The endangered plants designated and protected by the wildlife protection law, the Ministry of Environment, Korean were counted to one taxon; Cymbidium macrorhizum. The red list of vascular plants according to IUCN valuation basis were examined, endangered species (EN); Prunus yedoensis and Cymbidium macrorhizum, Vulnerable species (VU); Juniperus chinensis, Pulsatilla tongkangensis, Diarthron linifolium and Swertia wilfordii, Near Threatened species (NT); Paeonia japonica, Allium senescens, Epipactis papillosa and Pogonia minor, Least Concern species (LC); Asplenium ruta-muraria, Platycladus orientalis, Monotropa hypopithys and Eleutherococcus divaricatus var. chiisanensis, etc., Data Deficient species (DD); Paeonia lactiflora var. trichocarpa, and Not Evaluate species (NE); Prunus choreiana, Panax ginseng and Polygonatum infundiflorum etc. Korean endemic plants of this area were 26 taxa; Aconitum pseudolaeve, Corydalis hirtipes, Vicia chosenensis, Salvia chanroenica and Hemerocallis hakuunensis, etc. The floristic regional indicator plants found in this area were 130 taxa comprising 11 taxa of grade V, 15 taxa of grade IV, 33 taxa of grade III, 15 taxa of grade II, 56 taxa of grade I. The naturalized plants were identified as 67 taxa and the percent of naturalized index (NI) was 8.3% of total 804 taxa vascular plants.

• The Korean Journal of Ecology
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• v.14 no.2
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• pp.159-169
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• 1991

Floristic cmposition and soil properties were analyzed in chines cork oak(quercus variabilis)cmmunities in the limestone area, tanyang, ch'ungbuk province in korea. The tree layer was composed of quercus dentata, platycarya strobilacea, fraxinus rhynchophylla and others as wel as quercus variabilis. the shrub layer was dominated by ulmus davidiana for. suberosa,shrubby q. variabilis. euonymus alatus and rhus chinensis. among these, u. davidiana for. suberosa was known to have a restricted distribution to calcareous area, and 9 species in the shrub layer including e. alatus, indigofera kirilowii and rhamnus davurica belonged to the calcicole. The herb layer was dominated by carex lanceolata and spodiopogen cotulifer. ten specirs including g. lanceolata, clematis mandshurica, isachne globosa, lithospermum arvense and scabiosa mansenensis belonged to the calcicode. soil texture was classified to clay loam in both top and subsoil. Water cotent and organic matter were consistentl higher in top soil than in subsoil. soil ph rangd 7.8~8.4. Total n concentration in top soil ranged from 0.2 to 0.4mg/g, which was higher than that in subsoil. Available p and exchangeable k concentretion were also significantly higher in op soil than in subsoil. However, exchangeable ca concentration was similar betweenthe top and the subsoil. Unlike the other nutrients, exchangeable mg concentration in top soil was lower than that in subsoil. orangic matter, n, p and k content in this chinese cork oak stand showed as much as the other noncalcareous sites. Soil properties in this study area seemed to have been influenced by casts forming activities of earthworms.