• The Korea Journal of Herbology
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• v.25 no.4
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• pp.11-16
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• 2010

Objective : Oriental medicines have been combined oriental medical theory which based on the seven modes of emotions. Notopterygium incisum (N. incisum) and Clematis manshurica (C. manshurica) have been used as an anti-rheumatic and analgesic medicine for the treatment of rheumatism, headache, cold, etc. In this study, we evaluate the synergistic anti-inflammatory effect of N. incisum and C. manshurica. Method : To evaluate the synergistic anti-inflammatory effect of a herbal mixture N. incisum and C. manshurica, we examined the changed ear thickness in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema model after topical application of herbal mixture. In addition, the levels of markers for inflammation, such as tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$, prostaglandin $G_2$ ($PGE_2$), and nitric oxide (NO) were measured by ELISA assay and Griess reagent in lipopolysaccharide-stimulated Raw 264.7 cells. Results : Our results showed that aqueous extracts of N. incisum and C. manshurica combination significantly inhibited the mouse ear edema induced by TPA. Moreover, the aqueous extracts of N. incisum and C. manshurica combination exhibited synergistic effects in down-regulating TNF-${\alpha}$, IL-$1{\beta}$, $PGE_2$ level, but not NO. Conclusions : This study suggested that combined treatment of N. incisum and C. manshurica, based on seven methods in prescription compatibility, has a synergistic effect in down-regulating inflammatory response both in vitro and in vivo models.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.459-465
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• 1998

To obtain the basic data for the conservation and cultivation of native herb species under the forest, the relations between distribution of native herb species and site factors, distribution of native herb species, and site factors were surveyed on natural forest at subalpine zone in Mt. Odae. Ainsliaea acerifolia, Pseudostellaria palibiniana and Pimpinedla brachycarpa were distributed mainly on broadleaved mixed forest, Clematis fusca var. coreana, Carex siderosticta. Isodon excisus and Vicia unijuga did mainly on gab sites. The distribution of Ligularia fisheri, Aconitum pseudo-laeve var. erectum, Cacalia hastata and Artemisia stolonifera were significantly differed between slope directions. Artemisia stolonifera was distributed more frequently in south directed slope, and Ligularia fisheri, Aconitum pseudo-laeve var. erectum and Cacalia hastata were distributed more frequently in north and west directed slope than the other. The distribution of Aconitum pseudo-laeve var. erectum and Artemisia stolonifera were significantly differed between soil moisture conditions. Artemisia stolonifera was distributed mainly on the dry site, but Aconitum pseudo-laeve var. erectum did mainly on the dry site. Negative correlations between density of Clematis fusca var. coreana, Carex siderosticta. Isodon excises and Vicia unijuga and crown coverage was proved. Aconitum pseudo-laeve var. erectum, Pimpinella brachycarpa, Meehania urticifolia, Smilacina japonica and Pseudostellaria palibiniana were mainly distributed on wet and shady sites under forest canopy, but Pedicularis resupinata, Clematis fusca var. coreana and Artemisia stolonifera did on sunny sites at forest edge or gab.

• Korean Journal of Clinical Laboratory Science
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• v.53 no.2
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• pp.165-173
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• 2021

Clematis trichotoma Nakai (CTN) is a broad-leaved vine plant belonging to the family Ranunculus, native to Korea. Young leaves are used as food, and the stem and roots are used as medicinal materials. Antioxidant studies have been reported on the stems of CTN, but no studies have been conducted on the leaves. In this study, a 70% ethanol extract of CTN was prepared and its antioxidant and anti-inflammatory activities were investigated. For measuring the antioxidant activity, five assays (polyphenol and flavonoid content, reducing power, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity) were performed and CTN showed a concentration-dependent effect in all assays. To investigate the anti-inflammatory activity, we used RAW 264.7 cells. The concentrations (from 31.25 to 250 ㎍/mL) of CTN did not show cytotoxicity. CTN (250 ㎍/mL) inhibited dendritic transformation (34.4%) and also inhibited inflammation as seen by reduced levels of NO (77.4%), IL-6 (85.5%) and TNF-α (41.2%) compared to lipopolysaccharide (LPS). CTN (250 ㎍/mL) also suppressed the expression of the following genes: COX-2 (79.8%), iNOS2 (93.9%), IL-6 (87.6%), and TNF-α (77.3%) compared to LPS. These results demonstrated that CTN has excellent antioxidant and anti-inflammatory activities and can therefore be used as a natural biological resource.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.143.2-144
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• 2003

Joins (SKI 306X) is now clinically used for the treatment of osteoarthritis (OA). In previous reports, Joins a natural herbal product extracted from three herbs Clematis Radix. Trichosanthes Radix and Prunella Flos, was shown to have good analgesic and anti-inflammatory effects in several in vivo models. e.g., acetic acid-induced pain, carrageenan-induced paw edema and adjuvant-induced arthritis. (omitted)

• Korean Journal of Pharmacognosy
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• v.9 no.2
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• pp.57-72
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• 1978

The constituents of Ranunculaceae plants are summerized from more than one hundred of papers. Fifteen steroidal alkaloids, thirty two of terpenoids (diterpenes and triterpenes), fifty four isoquinoline alkaloids are recorded. Thirty three papaverines, eighteen berberines, and three hydrastines belong to isoquinoline group. Besides, of alkaloids a number of glycosides are also found. Adonis glycoside, famous cardiotonics, from Adonis and cyanogenin glycoside and calthoside D were identified from the leaves of Thalictrum aquilegifolium and Caltha silvestris. Anemonin, the irritating substance, found in Pulsatilla grandiz $W_{ENDER}$. Kaempf-erol and dihydrokaempferol were isolated and identified from the leaves of Clematis brachyura ^M_{AXIMOWICZ}$ by author in 1968.

• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.44-64
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• 1999

The flora of vascular plants investigated in Mt. Hyangro-Bong was 450 taxa belonging to 86 families, 254 genera, 393 species, 2 subspecies, 51 varieties, 4 formae. Among the investigated vascular plants, Korean endemic plants (Carex okamotoi, Salix koriyanagi, Clematis chisanensis, Aconitum pseudo-laeve var. erectum, Viola diamantiaca, Sanguisorba hakusanensis, Vicia venosissima, Patrinia saniculaefolia, Hanabusaya asiatica, H. asiatica for. alba etc.) were 23 taxa; rare and endangered plants(Disporum ovale, Lilium distichum, Trillium kamtschaticum, Gastrodia elata. Clematis chisanensis, Rodgersia podophylla, Rosa marretii, Viola diamantiaca, Syringa wolfii, Hanabusaya asiatica etc.) were 17 taxa; naturalized plants(Dactylis glomerata. Poa pratensis, Rumex acetosella, Gypsophila oldhamiana, Lepidium apetalum, Oenothera odorata, Aster pilosus, Carduus crispus, Erigeron annuus, Taraxacum officinale etc.) were 14 taxa.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.1-6
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• 2011

Objectives : Due to morphological similarity, many substitutes and counterfeits have been used as Moktong for thousands of years. This study was designed to determine discriminative criteria for identifying medicinal herbs used as Moktong. Methods : Akebia quinata, A. trifoliata, Clematis armandii, and Aristolochia manshuriensis were collected in various regions. Samples were authenticated and examined by observation and microscopy with paraffin embedding and triple staining using the modified Ju staining method. Results : Three different types of features to establish discriminative criteria for four kinds of Moktong were identified. Botanical features include differences in phyllotaxy, leaf shape, and caulescent. External morphological features were arrangement and size of fine holes(xylem), and overall shape of the section. Internal morphological features include the number of medullary rays, shape of the pitch, type of tissues rounding pitch, appearance of annual rings, shape and amount of crystals(calcium-oxalate), and the appearance of cork cambium. Further details(e.g. identification keys) are in the article. Conclusions : These criteria could provide an effective method for identifying numerous kinds of Moktong distributed in markets throughout northeast Asian nations.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.5
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• pp.17-35
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• 2011

The purpose of this study is to offer the raw data for management and conservation of national park by systematic and objective surveying and analysing the flora distributed in the Hwarang district, Gyeongju national park. The results are as follows. The vascular plants in this site were summarized as 396 taxa: 95 families, 272 genera, 351 species, 2 subspecies, 38 varieties and 5 forma. The rare plants designated by Korea Forest Service were 5 taxa: Exochorda serratifolia S.Moore, Potentilla discolor Bunge, Prunus yedoensis Matsum, Lysimachia coreana Nakai and Inula salicina var. asiatica Kitam.. The korean endemic plants were 6 taxa: Populus tomentiglandulosa T.B.Lee, Clematis trichotoma Nakai, Lespedeza maritima Nakai, Lysimachia coreana Nakai, Forsythia koreana (Rehder) Nakai and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region were 30 taxa: Asplenium sarelii Hk., Salix chaenomeloides Kimura, Lysimachia barystachys Bunge, Achillea alpina L., Celtis aurantiaca Nakai, Vitex negundo var. incisa (Lam.) C.B.Clarke and so forth. The plants with approval for delivering oversea were 6 taxa: Clematis trichotoma Nakai, Exochorda serratifolia S.Moore, Glycine soja Siebold & Zucc., Lespedeza maritima Nakai, Lysimachia coreana Nakai and Weigela subsessilis (Nakai) L.H.Bailey. The naturalized plants were 49 taxa: Bilderdykia dumetora (L.) Holub, Descurainia pinnata Britton, Oxalis corymbosa DC., Bidens frondosa L., Panicum dichotomiflorum Michx. and so forth. The invasive alien plants were 2 taxa: Ambrosia artemisiifolia L. and Aster pilosus Willd. In future, if we will survey the flora of whole Gyeongju national park, we will offer the help to establishing the conservation plan of ecosystem in Gyeongju national park.

• Korean Journal of Acupuncture
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• v.22 no.4
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• pp.109-116
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• 2005

목적 : 골관절염은 진통을 수반하는 퇴행성 관절질환이며, 장애를 일으키는 주요한 원인이 된다. 또한 노인들에 있어서 골관절염은 매우 흔한 질환이라 할 수 있다. Nitric oxide(NO)는 Nitric Oxide Synthase(NOS)에 의하여 칼슘의존성통로를 통하여 L-arginine 으로부터 합성되어지며, NO는 중추신경계에 있어서 중요한 세포사이의 전달자이다. 방법 :본 연구에서는 위령선 으로부터 추출한 액이 콜라겐으로 유도된 관절염에 걸린 쥐의 dorsolateral periaqueductal gray(DL-PAG) 영역에서 nNOS(neuronal NOS)와 NOS에 대하여 미치는 영향 을 nNOS immunohistochemistry와 nicotinamide adenine dinucleotide phosphate-diaphorase(NADPH-d) 검사법을 통하여 조사하였다. 결과 : 골관절염이 유발된 흰쥐의 DL-PAG 영역에서 nNOS와 NOS의 발현억제가 관찰되었으며, 위령선이 콜라겐으로 유도된 골관절염에서 감소된 nNOS와 NOS의 발현이 증가되었다. 결론 : 본 연구를 통하여 위령선은 골관절염이 유발된 흰쥐의 DL-PAG에서의 nNOS와 NOS의 발현에 영향을 미친다는 결과를 얻을 수 있었다.

• Korean Journal of Environment and Ecology
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• v.9 no.2
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• pp.202-210
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• 1996

To investigate edge vegetation struxture and species in Odaesan National park, fild survey was excuted July 1-4, 1995 and the results were as follows. Species compasition of edge(0~10m) was dissimilar to that of forest interior(50m~60m) at (Mt.)Odaesan valley. Species diversity indices, number of species, crown coverage and number of individuals were decreased axxording to distance from edge to forest interior. Weigela subsessilis was appeared to be a competitive species at forest edge in temperate forest and edge species appeared only in Odaesan National Park were Clematis heracleaefolia and Spiraea blumei.