• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.21 no.2
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• pp.99-102
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• 2011

Objectives: Acetone/triacetine method for clearing cellulose ester membrane (CEM) filter has been a popular method for air-borne asbestos analysis. However, as a weakness of this method, it is time consuming to analyses asbestos samples after sampling. Crystalclear method can be used to analyses asbestos samples promptly after sampling. Although a strength of crystal clear method exists, there was little valid studied for the method. This study was conducted to compare acetone/triacetine method with crystalclear one for analysing asbestos sample. Methods: Test samples made in three different concentration ranges(low, medium and high concentration) were analysed by phase contrast microscopy after acetone/triacetine and crystalclear method treatment respectively. Results: We did not find statistical difference in analysed results between two methods, which were conducted in three different concentrations ranges. Conclusions: We concluded that crystalclear method can be used as clearing method for air-borne asbestos analysis instead of acetone/triacetine method.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.125-138
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• 2024

Citrus yellow vein clearing virus (CYVCV) is a member of the Alphaflexiviridae family that causes yellow vein clearing symptoms on citrus leaves. A total of 118 leaf samples from nine regions of six provinces in Korea were collected from various citrus species in 2020 and 2021. Viral diagnosis using next-generation sequencing and reverse transcription polymerase chain reaction (RT-PCR) identified four viruses: citrus tristeza virus, citrus leaf blotch virus, citrus vein enation virus, and CYVCV. A CYVCV incidence of 9.3% was observed in six host plants, including calamansi, kumquat, Persian lime, and Eureka lemon. Among the citrus infected by CYVCV, only three samples showed a single infection; the other showed a mixed infection with other viruses. Eureka lemon and Persian lime exhibited yellow vein clearing, leaf distortion, and water-soak symptom underside of the leaves, while the other hosts showed only yellowing symptoms on the leaves. The complete genome sequences were obtained from five CYVCV isolates. Comparison of the isolates reported from the different geographical regions and hosts revealed the high sequence identity (95.2% to 98.8%). Phylogenetic analysis indicated that all the five isolates from Korea were clustered into same clade but were not distinctly apart from isolates from China, Pakistan, India, and Türkiye. To develop an efficient diagnosis system for the four viruses, a simultaneous detection method was constructed using multiplex RT-PCR. Sensitivity evaluation, simplex RT-PCR, and stability testing were conducted to verify the multiplex RT-PCR system developed in this study. This information will be useful for developing effective disease management strategies for citrus growers in Korea.

• Restorative Dentistry and Endodontics
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• v.30 no.6
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• pp.461-469
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• 2005

The aim of this study was to compare the root canal systems of maxillary and mandibular premolars that had a single root using radiographs and clearing samples. 142 single rooted premolars were selected and mesio-distal and bucco-lingual views were radiographed using intra-oral dental standard films. Four equally trained examiners classify the root canal types from the developed radiographs. After opening the tooth for access, it was stored in $5\%$ NaOCl to dissolve the pulp tissue. Indian ink was then injected into the pulp cavity to stain the pulp tissue. It was cleared in methyl salicylate after being decalcified with $5\%$ nitric acid for 48 hours, and the root canal type was evaluated at a magnification of $\times$ 20 using a stereomicroscope. The results are as follows ; There were statistically significant differences between the radiographs and clearing samples of the root canal types among examiners (p < 0.05). There might be differences in the root canal types among examiners when the same radiograph is used. Therefore, considering the difficulty in estimating the root canal types, clinicians need to be careful when interpreting radiographs before root canal therapy.

• Research in Plant Disease
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• v.26 no.4
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• pp.283-288
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• 2020

In September 2017, vein clearing and yellowing symptoms resembling those caused by viruses were observed on leaves of Malva verticillata in Chungnam, Korea. Nucleic acids were extracted from leaves of five symptomatic plants and tested by reverse transcription polymerase chain reaction using four virus specific primer pairs including malva vein clearing virus (MVCV). Amplicons of the expected size (600 bp) were obtained from total RNA of all samples using the MVCV-specific primers. To confirm the presence of MVCV in symptomatic plants, the DNA fragments from three samples were purified, and directly sequenced. BLAST analysis revealed that it shared the highest nucleotide identity (99%) with a MVCV isolate from tomato (Mexico). The virus isolates obtained from the third re-inoculated Chenopodium was designated as Cm1-5. Tissue from Cm1, Cm3, and Cm5 isolates was mechanically sap inoculated into 23 indicator plants. Cm3 isolate induced chlorotic local and mosaic symptoms in Althaea rosea. Phylogenetic analysis based on coat protein gene of 19 MVCV isolates from 6 different countries and plant species, did not correlated with either the geographical origin of the isolates, or pathogenicity. To our knowledge, this study first reports the natural occurrence of MVCV on M. verticillata in Korea and characterization of three Korean isolates of MVCV.

• Journal of the Optical Society of Korea
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• v.17 no.1
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• pp.86-90
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• 2013

Various methods have been proposed for enhancing the contrast of laser speckle contrast image (LSCI) in subcutaneous blood flow measurements. However, the LSCI still suffers from low image contrast due to tissue turbidity. Herein, a physicochemical tissue optical clearing (PCTOC) method was employed to enhance the contrast of LSCI. Ex vivo and in vivo experiments were performed with porcine skin samples and male ICR mice, respectively. The ex vivo LSCIs were obtained before and 90 min after the application of the PCTOC and in vivo LSCIs were obtained for 60 min after the application of the PCTOC. In order to obtain the skin recovery images, saline was applied for 30 min after the application of the PCTOC was completed. The visible appearance of the tubing under ex vivo samples and the in vivo vasculature gradually enhanced over time. The LSCI increased as a function of time after the application of the PCTOC in both ex vivo and in vivo experiments, and properly recovered to initial conditions after the application of saline in the in vivo experiment. The LSCI combined with the PCTOC was greatly enhanced even in deep vasculature. It is expected that similar results will be obtained in in vivo human studies.

• Journal of the Optical Society of Korea
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• v.17 no.4
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• pp.289-295
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• 2013

Although low-level laser therapy (LLLT) has been a valuable therapeutic technology in the clinic, its efficacy may be reduced in deep tissue layers due to strong light scattering which limits the photon density. In order to enhance the photon density in deep tissue layers, this study developed an optical tissue clearing (OTC) laser probe (OTCLP) system which can utilize four different OTC methods: 1) tissue temperature control from 40 to $10^{\circ}C$; 2) laser pulse frequency from 5 to 30 Hz; 3) glycerol injection at a local region; and 4) a combination of the aforementioned three methods. The efficacy of the OTC methods was evaluated and compared by investigating laser beam profiles in ex-vivo porcine skin samples. Results demonstrated that total (peak) intensity at full width at half maximum of laser beam profile when compared to control data was increased: 1) 1.21(1.39)-fold at $10^{\circ}C$; 2) 1.22 (1.49)-fold at a laser pulse frequency of 5 Hz; 3) 1.64 (2.41)-fold with 95% glycerol injection; 4) 1.86 (3.4)-fold with the combination method. In conclusion, the OTCLP system successfully improved the laser photon density in deep tissue layers and may be utilized as a useful tool in LLLT by increasing laser photon density.

• Journal of Photoscience
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• v.9 no.2
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• pp.448-450
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• 2002

DNA samples extracted from Japanese cypress leaf tissues contain isopropyl alcohol-precipitable, high molecular weight compounds, which interfere ELISA for cyclobutane pyrimidine dimers (CPD). Removal of the compounds is achieved by DEAE ion-exchange column chromatography and improves the ELISA responses of the DNA. When extracting DNA repeatedly from the same leaf tissues, the DNA samples show CPD responses which increase with the order in sequential extraction, and hence for a reliable detennination of DNA lesion a thorough extraction of DNA is required. Clearing these two problems it was demonstrated that CPD level was slightly higher in the leaves of trees growing under full sunlight than in those growing under UBV -cut sunlight.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.48 no.2
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• pp.107-114
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• 1999

This paper presents an application of artificial neural networks(ANN) to assess the dynamic security of power systems. The basic role of ANN is to provide assessment of the system's stability based on training samples from off-line analysi. The critical clearing time(CCT) is an attribute which provides significant information about the quality of the post-fault system behaviour. The function of ANN is a mapping of the pre-fault, fault-on, and post-fault system conditions into the CCT's. In previous work, a feed forward neural network is used to learn this mapping by using the generation outputs during the fault as the input data. However, it takes significant calculation time to make the input data through the network reduction at a fault as the input data. However, it takes significant calculation time to make the input data through the network reduction at a fault considered. In order to enhance the speed of security assessment, the bus data and line powers are used as the input data of the ANN in thil paper. Test results show that the proposed neural networks have the reasonable accuracy and can be used in on-line security assenssment efficiently.

• Advances in environmental research
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• v.4 no.4
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• pp.247-262
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• 2015

The few lignin biomarker studies conducted in tropical environments are hampered by having to use references signatures established for plants and soils characteristic of the temperate zone. This study presents a lignin biomarker analysis (vanillyls (V), p-hydroxyls (P), syringyls (S), cinnamyls (C)) of the dominant plant species and soil horizons as well as an analysis of the interrelated terrigenous organic matter (TOM) dynamics between vegetation and soil of the $Tapaj{\acute{o}}s$ river region, an active colonization front in the Brazilian Amazon. We collected and analyzed samples from 17 fresh dominant plant species and 48 soil cores at three depths (0-5 cm, 20-25 cm, 50-55 cm) from primary rainforest, fallow forest, subsistence agriculture fields and pastures. Lignin signatures in tropical plants clearly distinguish from temperate ones with high ratios of Acid/aldehyde of vanillyls ((Ad/Al)v) and P/V+S. Contrary to temperate environments, similarly high ratios in tropical soils are not related to TOM degradation along with pedogenesis but to direct influence of plants growing on them. Lignin signatures of both plants and soils of primary rainforest and fallow forest clearly distinguish from those of non-forested areas, i.e., agriculture fields and pastures. Attalea speciosa Palm trees, an invasive species in all perturbed landscapes of the Amazon, exhibit lignin signatures clearly distinct from other dominant plant species. The study of lignin signatures in tropical areas thus represents a powerful tool to evaluate the impact of primary rainforest clearing on TOM dynamics in tropical areas.

• The Korean Journal of Health Service Management
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• v.3 no.2
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• pp.45-53
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• 2009

This study tried to examine the conflict relationships and solutions among the members of a hospital structure, a structure composed of various experts and professionals. As the situations causing conflicts are very different depending on the sizes or structural types of hospitals, the samples were collected from total 235 people of 17 medical specialists, 46 residents, 74 nurses and 74 medical technicians, 40 administrators in a university hospital for clearing conflict relationships. The result showed that a hospital had separately different the conflict causes according to all types of occupations. That is, there were various conflict relationships such as the conflict with the medical specialists, with residents, with nurses, and with administrators, and resulted in respectively different causes from those conflict relationships. In addition to, looking into the top of conflict causes, the respondents in all occupations attributed the conflict with medical specialists to "authoritative attitude" of medical specialists, and attributed the rest types of conflict relationships to "lack knowledge of the work". And then, in terms of the top of conflict solutions according to the occupational types in the hospital structure, medical specialists and administrators chose competition & cooperation, and the way for residents, nurses and medical technicians was situation-avoidance. The competition & cooperation type, negotiation type and situation-avoidance type, among solution types, were little difference regardless of occupations, but in case of work-avoidance type, there were significant differences according to residents, nurses, medical technicians, administrators, and medical specialists in the order.