• 한국수자원학회논문집
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• 제38권3호
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• pp.245-257
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• 2005

본 연구에서는 격자로 분할된 유역에 ModClark 모형을 적용하여 수문모형의 매개변수를 산정하고 격자분할에 따른 유출변화에 대해서 검토하였다. 연구대상 유역은 IHP (국제수문개발계획)의 대표유역의 하나인 금강수계의 보청천 유역으로 하였다. 유역의 수문지형정보들은 HEC-GeoHMS를 이용하여 계산하였다. ModClark 모형은 격자형태의 강우자료를 요구하며 이 자료들은 HEC-DSS 파일 형태로 기록되어져 있어야 한다. 따라서 크리깅 방법을 이용하여 지점우량을 전체유역에 대한 격자 강우를 생성하였으며, Hec-DSSVue 프로그램을 이용하여 강우 입력자료를 DSS 파일로 생성하였다. 격자 크기 10000m, 5000m, 2000m 및 1000m에 대한 3가지 유출사상에 대해 모의하였으며, 모의 결과 첨두 홍수량, 유출체적, 첨두홍수 도달시간에 대해 실측수문곡선과 비교적 양호한 결과를 나타내었다.

• Journal of Advanced Marine Engineering and Technology
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• 제26권3호
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• pp.375-381
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• 2002

Measuring of the dissolved oxygen is widely used for the environmental control of natural waters, sewage waste treatment, medical and biochemical studies, soil husbandry, food and drug process control, and prevention of corrosion in boilers. Especially, a power plants need special management for preventing accidents from corrosion, therefore, it is essential to measure the concentration of dissolved oxygen in real-time. In this paper we present a method of measuring dissolved oxygen very accurately up to pub units. This method, called polarograph method, is based on the measures of the electric current generated by the oxidation process in cathode and de-oxidation process in anode, assuming that the amount of the current is proportional to the density of dissolved oxygen.

• 한국마린엔지니어링학회:학술대회논문집
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• 한국마린엔지니어링학회 2001년도 춘계학술대회 논문집
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• pp.197-201
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• 2001

Measuring of the dissolved oxygen is widely used for the environmental control of natural waters, sewage waste treatment, medical and biochemical studies, soil husbandry, food and drug process control, and prevention of corrosion in boilers. Especially, a power plants need special management for preventing accidents from corrosion, therefore, it is essential to measure the concentration of dissolved oxygen in real-time. In this paper we present a method of measuring dissolved oxygen very accurately up to ppb units. This method, called polarographic method, is based on the measures of the electric current generated by the oxidation process in cathode and de-oxidation process in anode, assuming that the amount of the current is proportional to the density of dissolved oxygen.

• 전기학회논문지
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• 제56권8호
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• pp.1450-1454
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• 2007

This paper presents a microfabricated Clark-type sensor which exactly can measure dissolved oxygen in the cell containing solution. We designed, fabricated, and characterized a microfabircated Clark-type oxygen sensor for measuring dissolved oxygen. The microfabricated oxygen sensor consists of 3-electrodes on a glass substrate, a FEP (Fluorinated ethylene propylene) oxygen-permeable membrane, and PDMS (Polydimethylsiloxane) reservoir for storing sample solution. Thin-film Ag/AgCl was employed as a reference electrode and its durability was verified by obtaining a stable open circuit potential for 2 hours against a commercial Ag/AgCl electrode and a stable cyclic voltammetry curve. Selectivity, response time, and linearity of the fabricated oxygen sensor were also verified well by cyclic voltammetry and amperometry depending. The fabricated oxygen sensor showed a 90% response time of 40sec and an excellent linearity with a correlation coefficient of 0.994.

• 한국습지학회지
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• 제13권1호
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• pp.105-116
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• 2011

최근 몇 년간 기후변화에 의해 기상이변이 발생하고 있으며 이에 따른 집중호우로 인한 홍수피해가 심각하게 증가하고 있다. 이러한 피해를 저감하기 위한 수문기상학적 요소와 특성인자들의 정확한 상호 연관성 규명과 공간적 변동성 해석은 강우-유출 모형에서 발생하는 불확실성을 감소시키는데 중요한 요소로 작용하게 된다. 이에 본 연구에서는 레이더강우 격자 해상도와 지형인자 격자 해상도에 따라 강우-유출모형이 어떻게 반응하는지 분석하였으며, 가-분포 강우-유출 모형인 ModClark 모형을 이용하여 강원도 인제군의 내린천 유역을 대상으로 광덕산 레이더자료를 이용하였다. ModClark 모형 구성을 위한 GIS 지형공간 자료는 30m, 150m, 250m, 350m 격자크기의 DEM을 사용하였으며, 2006년 7월 14일부터 7월 17일까지의 관측레이더 강우자료를 500m, 1km, 2km, 5km, 10km 사용하여 유출모의를 실시하고, 각각의 격자해상도에 따른 모의 결과를 비교하기 위해 유출 수문곡선을 작성하고 유출량 변화를 모의하였다. 분석 결과 첨두유량 및 유출체적에 대해서는 DEM 30m~150m, 레이더강우 500m~2km 크기의 격자일 때 가장 최적의 유출 모의를 한 것으로 분석되었으며, 통계적 분석에 의한 분석결과에서는 모든 DEM 격자는 레이더강우 격자가 500m인 경우, 모든 레이더강우 격자는 DEM 30m인 경우에 모형의 적합성이 높은 것으로 나타났고, 민감도 산정 결과 지수 등급이 높은 DEM이 분포형 모형의 결과 값에 큰 영향을 주는 것으로 분석되었다. 최근 집중형 모형에서 분포형 모형을 이용한 강우-유출해석이 이루어지고 있기에 모델링 구성을 위한 효율적인 의사결정의 기준으로 활용될 수 있을 것으로 기대된다.

• 약학회지
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• 제51권2호
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• pp.145-149
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• 2007

Saussurea Radix, the dried roots of Saussurea lappa Clark (Compositae), has been used in oriental traditional medicine for aromatic stomachic. Present study was carried out for the anticancer effect of Saussurea Radix. Bioassay-directed fractionation of Saussurea Radix led to the isolation of two sesquiterpenes, dehydrocostuslactone (1) and costunolide (2). The structures of 1 and 2 were elucidated by spectral methods (MS, IR, $^1$H and $^{13}$C NMR). These compounds showed a potent cell proliferation activity against human breast cancer cell MCF-7 and MDA-MB-453.

• 한국항공우주학회지
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• 제40권10호
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• pp.846-852
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• 2012

본 연구는 모핑 항공기 날개를 설계/제작하기 위한 선행 연구로서 기본날개단면 및 모핑날개단면에 대한 공력특성을 실험적으로 조사하였다. 이를 위해 Clark-Y형 에어포일을 가진 기본날개, 기계식 플랩을 가진 날개, 모핑플랩을 가진 날개를 제작하여 풍동실험을 수행하였다. 3축 로드셀을 이용하여 날개에 작용하는 양력, 항력 및 피칭모멘트를 측정하였으며, 풍동실험데이터는 Solid Blockage 와 Wake Blockage를 고려하여 보정하였다. 풍동 실험은 각 날개별로 다양한 속도, 레이놀즈, 받음각에 대해 수행되었다. 실험결과는 모핑 에어포일의 양력-항력 및 양력 피칭모멘트 특성이 기계식 플랩을 가지는 에어포일에 비해 우수한 것을 보여준다.

• Journal of Korean Neurosurgical Society
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• 제63권6호
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• pp.707-716
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• 2020

Objective : The function of B7H3, a member of the B7 family of proteins, in neuroblastoma (NB) remains poorly characterized. Here we examine the expression pattern of B7H3 in clinical NB specimens and characterize the phenotype of B7H3 knock-down in NB cell line. Methods : Immunohistochemical (IHC) staining was carried out to assess the expression of B7H3 in clinical NB specimens. Survival association was analyzed using five Gene Expression Omnibus (GEO) datasets (GSE85047, GSE45480, GSE62564, GSE16476, GSE49710). Clonogenic survival and flow cytometry were performed after B7H3 knockdown to assess the cellular proliferation and cell survival in vitro. Impact of B7H3 silencing on NB growth was examined in vivo using the SH-SY5Y xenograft model. Results : On IHC staining, B7H3 was widely expressed in clinical NB specimens. Analysis of the transcriptional profiles of five GEO datasets clinically annotated NB specimens revealed that decreased B7H3 expression was associated with improved overall survival. B7H3 knockdown suppressed the proliferation of the SH-SY5Y NB model in vitro and in vivo. Cell cycle analysis revealed that B7H3 silencing induced G1/S arrest. This arrest was associated with the suppression of E2F1 expression and induction of Rb expression. Conclusion : Our results demonstrate that B7H3 expression correlate with clinical survival in NB patients. Preliminary studies suggest that B7H3 may mediate the G1/S transition.

• The Korean Journal of Physiology
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• 제15권1호
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• pp.3-8
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• 1981

Studies have been conducted to test the effect of Ginseng alcohol extract on the membrane potentials of frog skeletal muscle. The gastrocnemius muscle was isolated and placed in a chamber containing the Clark-frog Ringer solution. Membrane potentials were recorded using microelectrodes filled with 3 M KCI and muscle was electrically stimulated to obtain action potential. Changes in both the action potential and the resting membrane potential were observed after adding an appropriate amount of Ginseng alcohol extract in the perfusing Ringer solution. The results obtained from 346 muscle cells are summarized as follows : 1) The average resting membrane potential of the normal frog gastrocnemius muscle cell was -92.8 mV and the peak of the action potential reached at 29.8 mV. 2) Both the resting membrane potential and the peak of the action potential decreased by Ginseng alcohol extract, the effect being proportional to the dose of Ginseng alcohol extract. 3) The resting membrane potential and the peak of the action potential continuously decreased until about 40 min after Ginseng addition and leveled off thereafter. The potentials recovered to its original value after Ginseng was washed out. 4) The resting membrane potential was more sensitive to the Ginseng alcohol extract than was the action potential. These results strongly suggest that Ginseng alcohol extract increases both the $Na^+$ and $K^+$ permeability in the skeletal muscle cell membrane.

• Asian Pacific Journal of Cancer Prevention
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• 제15권19호
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• pp.8161-8169
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• 2014

Background: Cancer stem cells (CSC) are populations of cells responsible for tumor initiation, progression and therapeutic resistance in many cancers. In the present study, we aimed to investigate the expression pattern and clinical significance of two CSC markers, CD133 and Nestin, in a series of skin tumors. Materials and Methods: One hundred and thirteen paraffin blocks from skin cancers including 16 (14%) cases of melanoma, 37 (33%) of squamous cell cancer (SCC) and 60 (53%) of basal cell cancer (BCC) were collected and assembled in a tissue microarray (TMA). The samples were immunohistochemically examined for the expression of CD133 and Nestin. Expression of these markers was also correlated with clinicopathological parameters. Results: A significant difference was observed in the expression of CD133 and Nestin in melanomas, SCC and BCC (p value=0.001). Furthermore, the level of expression was significantly higher in the melanomas compared to the SCC and BCC tumors. Expression of CD133 in the melanoma was significantly associated with increased tumor invasiveness (p value=0.05), a higher rate of metastasis (p value=0.04) and the presence of ulceration (p value=0.02). Increased expression of Nestin was observed in metastatic melanoma (p value=0.04), while no statistically significant correlation was found with other clinicopathological parameters including Breslow thickness, Clark level and ulceration. Conclusions: Elevated expression levels of CD133 and Nestin in the melanomas are associated with advanced disease, with more aggressive and metastatic skin tumors. Therefore, these markers could be potential therapeutic targets for malignant tumors of the skin.