• 생명과학회지
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• 제26권6호
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• pp.657-662
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• 2016

본 연구에서는 큰실말의 지질대사 효과를 연구하기 위하여 체중 158±10 g의 SD계 암컷 흰쥐 25마리를 대상으로 정상군, 고지방식이 대조군, 큰실말 섭취군, 큰실말 불용성 식이섬유 섭취군, 큰실말 수용성 식이섬유 섭취군으로 분류하여 52일간 사육하였다. 체중은 큰실말 섭취군이 고지방식이군에 비하여 유의적으로 감소하여 정상군에 가까운 수치를 나타냈다. 혈액에서의 HDL 수치는 대조군보다 큰실말 및 식이섬유를 섭취한 군의 수치가 더 높게 나타났고, TC, TG, LDL, GOT, GPT의 수치는 대조군보다 큰실말 및 식이섬유를 섭취한 군의 수치가 더 낮게 나타났다. 이와 같은 연구를 통해 큰실말 및 식이섬유가 혈중 콜레스테롤 농도를 개선시켜 지질 대사에 미치는 순기능적 효과를 보여주는 것으로 사료된다.

• ALGAE
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• 제24권2호
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• pp.79-83
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• 2009

Recently deschbed new species, Cladosiphon umezakii Ajisaka (Ectocarpales,Phaeophyceae) is reported in Korea based on morphology and plastid rbcL sequences. Cladosiphon umezakii occurred on low intertidal to subtidal zone along the south and southeast coasts in Korea. Thalli are gold to dark brown, ercct, cylindrical, irregularly branched,and very mucoid. Unitocular zoidangium is developed at the base uf assimilatory filaments. Assimilatory filaments are very long. Eight specimens of the pecies collected from Korea clustered with those of C. umezakii in Japan in rbcL tree. The pecies showed a sister relationship with C. okamuranus. The occurrence of this warm water species is thought an example of northward migration of marine algae caused by global warming. Because C. umezakii is of the economica! importance of the genus as foods and medidnes, the study on mass culture of the species is necessary in korea.

• 대한화장품학회지
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• 제42권3호
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• pp.285-295
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• 2016

큰실말(Cladosiphon novae-caledoniae kylin, C. novae-caledoniae kylin) 부산물을 이용하여 화장품 소재로써 가능성을 조사하고자, 항산화 효과(DPPH 자유 라디칼 소거 활성), 항균(anti-microbial activity), 미백(tyrosinase inhibition assay), 주름개선(elastase inhibition assay) 및 B16F10 mouse melanoma cell (MTT assay)을 이용한 세포 생존률을 측정하였다. 큰실말 추출물 원물(MC)과 불용성(MI)은 Staphyloccus aureus (S. aureus)와 항생제내성균주인 MRSA에 대하여 활성을 나타내었지만 항곰팡이 활성은 나타내지 않았다. 큰실말 추출물 수용성(MS)은 tyrosine에 대한 tyrosinase 저해효과가 49%로 표준물질인 arbutin 보다 미백효과가 우수하였지만 주름개선효과는 비교적 낮은 활성을 나타내었다. DPPH 라디칼 소거능력은 $500{\mu}g/mL$에서 89%로 나타내었다. 따라서 본 연구인 큰실말 추출물은 화장품 미백소재로써 좋은 후보군임을 확인할 수 있었다.

• 한국식품위생안전성학회지
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• 제29권4호
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• pp.370-375
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• 2014

Cladosiphon okamuranus is edible brown algae cultured commercially and massively at Okinawa coast. Dietary fiber occupying most of C. okamuranus isn't digested and absorbed by digestive enzymes of human. But it is known to prevent and treat constipation as metabolic disease. This study was to investigate the effects of dietary fiber extracted from C. okamuranus on the damage of liver and the blood cholesterol level in the high fat diet-fed rats. The effects were measured by the levels of aspartate aminotransferase (ALT), alanine aminotransferase (AST), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL) in sera. The levels of ALT, AST, TC, TG and LDL were significantly reduced in the C. okamuranus-treated group compared with the high fat diet group. But HDL level was markedly increased. The results showed that the dietary fiber extracts from C. okamuranus have the inhibitive effects of cholesterol biosynthesis in the high fat diet-fed rats.

• 공업화학
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• 제25권4호
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• pp.363-367
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• 2014

본 연구에서는 큰실말 부산물의 추출 조건(HCl, $H_2SO_4$, NaOH, $Na_2CO_3$, $Na_2EDTA$)에 따른 식이섬유 함량을 조사하였다. 또한 큰실말 부산물로부터 분리한 식이섬유의 총 폴리페놀과 플라보노이드양을 측정하고 HT-29 대장암 세포에서의 증식 억제를 통한 항암효과를 확인하였다. 1.5% $Na_2EDTA$와 0.05 N HCl이 큰실말 부산물로부터 가용성 식이섬유를 추출하는데 효과적이었으며 추출 시간과 온도도 가용성 식이섬유의 추출수율에 영향을 주었다. 큰실말 부산물 식이섬유의 총 폴리페놀과 플라보노이드 함량은 1% NaOH 추출물에서 가장 높게 측정되었다(총 폴리페놀 $34.4{\pm}0.055$ mg gallic acid/g dry basis, 총 플라보노이드 $34.7{\pm}0.023$ mg naringin/g extract dry basis). 또한 DPPH 라디칼 소거능 측정에서도 1% NaOH 추출물에서 가장 높은 항산화 효과를 보였다. 대장암 세포주의 세포 생존율 측정 결과, D.W.와 0.05 N HCl 및 0.5% $Na_2CO_3$ 추출물에서 농도의존적 세포 증식 억제를 보였다. 이들 결과는 큰실말 부산물로부터 분리한 가용성 식이섬유가 항산화 및 대장암에서의 항암효과에 중요한 기능이 있음을 시사한다.

• Food Science and Biotechnology
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• 제16권2호
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• pp.212-217
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• 2007

Fucoidan (fucan sulfate) is a fucose-containing sulfated polysaccharide from brown algae such as Fucus vesiculosus, Ecklonia kurome, and Cladosiphon okamuranus. The aim of this study was to investigate the effect of fucoidan on the expression of diabetes-related genes in mouse cell line 3T3-L1. 3T3-L1 adipocytes were cultured for 48 hr with or without fucoidan (10, 100, and 500 ppm) on a 60 mm dish. Reverse transcription polymerase chain reaction (RT-PCR) was used for measurement of peroxisome proliferators activated receptor ${\gamma}\;(PPAR{\gamma})$, CCAAT/enhancer binding protein ${\alpha}\;(C/EBP{\gamma})$, and glucose transporter 4 (GLUT4) RT-PCR analysis revealed that expression level of GLUT4, $PPAR{\gamma}$, and $C/EBP{\alpha}$ mRNAs increased with fucoidan treatment from 10 to 500 ppm in a dose-dependent manner. Fucoidan appears to enhance insulin sensitivity by increasing the expression level of diabetes-related genes in 3T3-L1 adipocytes. Therefore, fucoidan is potentially useful as a natural therapeutic material for hyperglycemia in type II diabetes patients.

• 농업과학연구
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• 제36권1호
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• pp.41-50
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• 2009

Fucoidan is a uniquely-structured sulfated fucose-rich polysaccharide derived from brown algae. Recently, the abalone glycosidase-digested fucoidan extract (fucoidan extract) derived from seaweed Cladosiphon novae-caledoniae Kylin (Mozuku) draws much attention because of its clinical anti-cancer effect in Japan. Here, we report the cancer cells-specific apoptosis inducing effects of the fucoidan extract. The fucoidan extract suppressed the growth of various anchorage-dependent and -independent cancer cells. The fucoidan extract contained low molecular weight components, which induced apoptosis of human leukemic HL 60 cells but not of human lymphocytes. It was shown that the fucoidan extract lead caspase 3/7 activation and loss of mitochondrial membrane potential in HL 60 cells. Another function of the fucoidan extract was also observed. It has been known that sugar chain expression on the surface of cancer cell membrane changes dependent on their malignancy. The analysis on sugar chain expression profiling using FITC-labeled lectins revealed that the expression of concanavalin A (Con A) binding sugar chain was enhanced by the treatment of human lung adenocarcinoma A549, human uterine carcinoma HeLa and human fibrosarcoma HT1080 cells with the fucoidan extract. Con A-induced apoptosis of cancer cells was stimulated in a dose-and time-dependent manner by the treatment with the fucoidan extract but not of human normal fibroblast TIG-1 cells.