• Korean journal of applied entomology
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• v.17 no.1 s.34
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• pp.7-13
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• 1978

Experiments were conducted to study the integrated control of one of the major pests of citrus, the citrus red mite, Panonychus citri (McGregor). Studies were conducted in Seogwipo, Cheju Island, 1973-1976. Results obtained were: 1. The major peak of citrus red mite occurrence was mid-July to mid-August. 2. Standard field populations of citrus red mite were also high in September, October, and November. 3. A total of 10 species of natural enemies of citrus red mite were found. These included; Oligota yasumatusi Kistner, Anystis baccarum L., Hemerobiid sp., Semidalis albate E., Orius sp., Agistemus terminalis Q., 3 species of lady beetles (Coccinellidae), and one unidentified species of predacious mite. 4. Annual occurrence of citrus med mite tended to decrease in unsprayed fields, but increased in fields receiving standard treatments. 5. With fungicides, there was an increase in mite populations associated with use of Bordeaux mixture and copperpowder sprays. Streptomycin, however, did not effect on increase in mite numbers.

• The Korean Journal of Pesticide Science
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• v.4 no.4
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• pp.87-92
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• 2000

Recently, a new developed petroleiun spray oil(PSO; D-C Tron $Plus^{(R)}$) has been used to control key pests in integrated pest management (IPM) system of citrus orchards in Australia. The efficacy and phytotoxicity of the PSO against the citrus red mite (Panonychus citri) were compared with conventional pesticides (e.g.Tebufenpyrad, Bifenazate and Sun spray oil) in field condition in Jeju. And under PSO mixing spray with some fungicides, the occurrence of phytotoxic symptoms on citrus leaf was investigated. All concentrations of PSO spraying (0.25%, 0.33%, 0.5% and 1.0%) were significantly suppressed the citrus red mite to similar levels of other conventional pesticides. And the spraying of PSO levels ${\geq}0.5%$ was induced not only occurrence of some oil-sucked symptoms on leaf, but also increase of the dropping leaf and fruit rates. As results from mixing PSO spraying test with other fungicides, little burning on new flush shoot was founded only in PSO 1% plus Fluazinam treatment. From the results of this study, with the consideration of pesticidal efficacy and phytotoxicity, 0.25% and 0.33% PSO spraying level will be recommended for the control of citrus red mite during summer season in Jeju.

• Korean journal of applied entomology
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• v.37 no.2
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• pp.193-198
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• 1998

Experiments were conducted to investigate the population fluctuation, developmental periods, fecundity, hatching rate and demage of Citrus red mite (Panonychus citri M.) on Yuzu trees (citrus junos T.) from 1996 to 1997. Citrus red mite occurred from May to November and made two peaks. The first peak was in July to August and the secondary peak was in October. Density of the second peak was higher (9.5 miteslleaf) than that of the first peak. In the constitution rate of each developmental stage of citrus red mite on Yuzu leaves, egg stage occupied 85%. At the four constant temperature (15, 20, 25, 30 + 1$^{\circ}$C, RH 60 + lo%, 14L- IOD) conditions, the developmental period from egg to adult was 41 .l, 15.5, 11.0 and 9.4 days ; Mean longevity of adult was 23.3, 8.3, 6.3, and 6.1 days; Mean number of egg laid per female per day was 1.6, 3.2, 4.5, 4.0 eggs; Mean hatching rate was 66.6, 85.7, 90.7 and 94.7% at above temperature, respectively. When sprayed acaricide at different density of Citrus red mite, the growth of young Yuzu tree were better at low density. Defoliation rate during winter was 13.5, 20.6, 53.1, 72.6% at 4 control density 1 , 3, 6 mites per leaf and uncontrolled (10 ( ). When every time spray acaricide under 3 mites per leaf, defoliation rate during winter suppressed above 50% compare to uncontrol 72.6%.

• Korean journal of applied entomology
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• v.40 no.2
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• pp.105-109
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• 2001

Dispersion pattern of the citrus red mite (CRM), Panonychus citri (McGregor) was determined to develop a monitoring method in the satsuma mandarin fields, Citrus unshiu L., in Jeju-do, during 1999 and 2000. CRM population was sampled by collecting leaves. Taylor's power law provided better description of mean-variance relationship for the dispersion indices compared to Iwao's patchiness regression. Slopes and intercepts of Taylor's power law from leaf samples did not differ among surveyed groves. Fixed-precision levels (D) of a sequential sampling plan were developed using Taylor's power law parameters generated from all motile stages of CRM in leaf sample. This sampling plan for leaf sample estimate was tested with resampling validation for sampling plan using 4 independent data sets. Resampling simulation analysis demonstrated that actual fixed-precision level values were better than desired D values of 0.20, 0.25 and 0.30. Required numbers for tree sampling at the density of more than 7 mites per tree were fewer than 18.

• Korean journal of applied entomology
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• v.39 no.1
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• pp.13-19
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• 2000

This studies was carried out to investigate the natural enemies of citrus red mite, pononychus citri and seasonal occurrence of its major beneficial insects in Yuzu groves (Chonnam province) from 1996 to 1998. Natural enemies against citrus red mite were 9 species including Oligota kashmirica benifica, 0. yasumatsui, Stethorus punctillum, Chrysopa pallens, Propylea japonica, Orius sauteri, Scolothrips takahashii, Amblyseius womersleyi and one unidentified species of thrips (Family: Phlaeothripidae). Among them, Oligota kashmirica benzficu and Stethorus punctillum were found to be dominant species. 0 . kushmirica henifica had 4-5 generations from middle May to middle November with peak in early September. S. punctillum had 3 generations from late May to late October with peak of late June to middle July. Population dynamics between predator ( 0 . kushmiricu benifica and S. punctillum) and prey (Panonychus citri) were quite well synchronized until September but predator density was decreased abruptly after October.

• Parasites, Hosts and Diseases
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• v.52 no.1
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• pp.117-120
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• 2014

Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify and assess properties of a cysteine protease from the mites acting as a potentially pathogenic factor to citrus-cultivating farmers. A cysteine protease was purified using column chromatography of Mono Q anion exchanger and Superdex 200 HR gel filtration. It was estimated to be 46 kDa by gel filtration column chromatography and consisted of 2 polypeptides, at least. Cysteine protease inhibitors, such as trans poxy-succinyl-L-leucyl-amido (4-guanidino) butane (E-64) and iodoacetic acid (IAA) totally inhibited the enzyme activities, whereas serine or metalloprotease inhibitors did not affect the activities. In addition, the purified enzyme degraded human IgG, collagen, and fibronectin, but not egg albumin. From these results, the cysteine protease of the mites might be involved in the pathogenesis such as tissue destruction and penetration instead of nutrient digestion.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.29-34
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• 2003

Dispersion pattern for adult citrus red mite (CRM), Panonychus citri (McGregor) using by Taylor's power law (TPL) and Iwao's patchiness regression (IPR) was determined to develop a monitoring method on citrus orchards, on Jeju, in Autumn season, during 2001 and 2002.CRM population was sampled by collecting leaves and fruits. The relationships of CRM adults between leaf and fruit were analyzed by different season. The regression equation for CRM adults between leaf (X) and fruit (Y) was ln(Y+1) : 1.029 ln(X+1) ( $r^2$ : 0.80). The density of CRM was higher on fruit than on leaf according to fruit maturing level. TPL provided better description of mean-variance relation-ship for the dispersion indices compared to IPR. Slopes and intercepts of TPL from leaf and fruit samples did not differ between sample units and surveyed years. Fixed-precision levels (D) of a sequential sampling plan were developed using Taylor's power law parameters generated from adults of CRM in leaf sample. Sequential sampling plans for adults of CRM were developed for decision making CRM population level based on the different action threshold levels (2.0,2.5 and 3.0 mites per leaf) with 0.25 precision. The maximum number of trees and required number of trees sampled on fixed sample size plan on 2.0,2.5 and 3.0 thresholds with 0.25 precision level were 19, 16 and 15 and their critical values T$_{critical}$ at were 554,609 and 659, respectively. were 554,609 and 659, respectively.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.197-202
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• 2001

The density of citrus red mite(CRM), Panonychus citri(McGregor), on the commercial satsuma mandarin Citrus unshiu L. groves were determined by counts of the number of CRM per leaf using by leaf sample in Jeju for 2 years. Binomial sampling plans were developed based on the relationship between the mean density per leaf(m) and the proportion of leaf infested with less than T mites per leaf($P_{T}$), according to the empirical model $ln(m)={\alpha}+{\beta}ln(-ln(1-P_{T}))$. T was defined as tally threshold, and set to 1, 3, 5 and 7 mites per leaf in this study. Increasing sample size, regardless of tally threshold, had little effects on the precision of the binomial sampling plan. Increasing sampling size had little effect on the precision of the estimated mean regardless of tally thresholds. T=1 was chosen as the best tally threshold for estimating densities of CRM based on the precision of the model. The binomial model with T=1 provided reliable predictions of mean densities of CRM observed on the commercial satsuma mandarin groves. Binomial sequential sampling procedure were developed for classifying the density of CRM. A binomial sampling program for decision-making CRM population level based on action threshold of 2 mites per leaf was obtained.

• Korean journal of applied entomology
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• v.4
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• pp.47-50
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• 1965

Many of the chlorophenol derivatives are widely used as insecticides, herbicides and plant growth regulators. The function and use of these chemicals would be different according to the number of chlorine and their chemical structures. It was reported in this article that 1) Bis(trichlorophenoxy) ethane was synthesized with 2 mol-trichlorophenol and 1 moi-dibromoethane in 2 moi-Sodium hydroxide alcohol solution, and 3) the insecticidal activity of Bis(trichlorophenoxy) ethane was compared with Phenkapton, Tedion V-18, BHC, and DDT, on Citrus red mite, Bombix mori (Silk worm), and Daikon leaf beetle(Phaedon brassicae) 3) The toxicity of Bis(2, 4, 5-trichlorophenoxy) ethane and Bis(2, 4, 5-trichlorophenoxy) ethane was studied on mice. The following results were obtained. 1) Yield of Bis(2, 4, 5-trichlorophenoxy) ethane, $50.06\%$, m.p. $157-159^{\circ}C$, and yield of Bis(2,4, 6-trichlorophenoxy) ethane, $32.60\%$, m.p. $162-163^{\circ}C$. 2) Insecticidal activity of Bis(2, 4, 5-trichlorophenoxy) ethane to Citrus red mite is stronger than that of Tedion V-18, and weaker than that of Phenkapton. 3) Insecticidal activity of Bis(2, 4, 5-trichlorophenoxy) ethane to Bombix mori is weaker than those of BHC and DDT. 4) Insecticidal activity of Bis(2. 4, 5-trichlorophenoxy) ethane to Daikon leaf beetle proved to be ineffective. 5) Five rams of Bis(2, 4, 5-trichlorophenoxy) ethane and Bis(2, 4, 6-trichlorophenoxy) ethane pet kg of body weight respectively were given to mice orally, and none of the mouse was killed by it after a period of 72 hours. Therefore it seems that there is almost no toxicity.

• Korean journal of applied entomology
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• v.44 no.2
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• pp.125-130
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• 2005

The ecological characteristics of the specialist insect predator, Oligota kashmirica benefica, were investigated for developing artificial rearing method with special interest on the oviposition behavior, the pattern of adult emergence and temperature in storage, and its effect on the density of the citrus red mite, Panonychus citri. Female beetle oviposited eggs mainly, $95\%$ of the eggs, on the bottom of leaves of the yuzu tree, and $91.3\%$ of them were covered with ecdysis skin of the red mites or the feces of themselves. The rate of adult emergence of the beetle rose up to $86.7\%$ when the horticultural media was provided for pupation from $60\%$ when it pupated in upland soil. Most larvae, $88\%$ of them, were found from the surface to the depth of 2 cm in the horticultural media. The optimum temperature for the storage of the adult beetle was found to be $12^{\circ}C$, at this temperature the 96.7, 73.3 and $70.0\%$ of the beetle survived for 10, 20, and 30 days, respectively. The control effect of the citrus red mite was appeared highest by releasing the beetle at the rate of one beetle against 150 mites.