• Title/Summary/Keyword: Citric acid (CA)

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Effect of Ca^{++} and Cu^{++} Removal from Molasses on Yest cell Growth and RNA Accumulation. (당밀로부터 Ca^{++} 및 Cu^{++} 이온 제거가 효모 생육 및 RNA축적에 미치는 영향)

  • 김재범;허선연;김중균;남희섭;남수완
    • Microbiology and Biotechnology Letters
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    • v.31 no.3
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    • pp.211-215
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    • 2003
  • When Saccharomyces cerevisiae MTY62, a high-RNA content yeast, was cultivated by fed-batch mode feeding molasses and com steep liquor, the cell density less than 45g-DCW/L and the RNA content less than 140mg/g-cell were obtained, indicating that unknown compounds inhibiting the cell growth and RNA accumulation are contained in the molasses. Therefore, in order to obtain higher levels of cell density and RNA content, $Ca^{++}$, $Cu^{++}$and $K^{+}$ ions in molasses were removed by pretreatments of molasses with various agents such as IonClear BigBead, $Na_2$$HPO_4$, $H_2$$SO_4$, citric acid, $K_2$$HPO_4$, and EDT A. Among them, IonClear BigBead, $Na_2$$HPO_4$, and EDTA gave the highest $Ca^{++}$ removal efficiency of about 60-90%. In the batch culture with pretreated molasses, the cell concentration of 18.6g-DCW/I and RNA concentration of 3127 mg/I, maximum specific growth rate of 0.459$h^{-1}$ , and specific consumption rate of reducing sugar of 1.28g-sugar/g-cell-h were obtained, which are about 10%, 17%,47%, and 36% higher levels, respectively, over the batch culture with untreated molasses.

Prevention of Quality Changes in the Cultured Wild Ginseng During Storage (산삼배양근의 저장 중 품질변화 억제)

  • Whang, Jong-Hyun;Yu, Kwang-Won;Park, Sung-Sun;Koh, Jong-Ho;Oh, Sung-Hoon;Suh, Hyung-Joo;Lee, Sang-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.10
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    • pp.1312-1317
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    • 2008
  • Physicochemical changes were investigated for the shelf-life extension of cultured wild-ginseng roots during storage with various pre-treatments with blanching, CAMICA-SD and DF-100 and treatments with citric acid and vitamin C. The pH of cultured wild-ginseng roots showed the range of $6.06{\sim}6.42$ at $10^{\circ}C$, but showed higher ranges of $6.08{\sim}6.91$ and $6.08{\sim}8.68$ at 20 and $30^{\circ}C$, respectively. Browning index (a/b) was increased with increasing storage temperature, and the index at 10 and $30^{\circ}C$ were 0.405 and 0.469 after 2 weeks, respectively. Browning index and viable cell number of CAMICA-SD pre-treatment showed little changes compared to pre-teatment with blanching or DF-100. When the cultured wild-ginseng roots were treated with 1.0% citric acid and 0.2% DF-100 after pre-treatments with CAMICA-SD, viable cell number was slightly increased to $4.9{\times}10^2CFU/g$ for 3 weeks storage at $10^{\circ}C$. The mixture of citric acid and DF-100 was also used to prevent the growth of microbiology and to reduce browning reaction, especially enzymatic browning reaction. The mixture might effectively extend shelf life of the cultured wild-ginseng roots.

UV-cut Finish of Cotton Using Polycarboxylic acid and Vitamin C (폴리카르복시산과 Vitamin C를 이용한 면섬유의 자외선 차단 가공)

  • Lee, Sang-Uk;Choi, Min-Young;You, Mi-Dum;Jang, Jin-Ho
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2011.11a
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    • pp.30-30
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    • 2011
  • 산업화 과정에 방출된 할로겐 화합물 및 프레온 가스가 오존층을 파괴하면서 많은 양의 자외선이 지표에 도달하여 생태계에 적지 않은 영향을 끼치고 있다. 자외선은 살균, 소독작용 및 Vitamin D 합성을 위해 생명체에 필수적이지만 과다할 경우 색소침착, 피부노화, 피부암 발생, 백내장의 증가, 면역기능저하 등의 피해를 준다. 자외선은 파장 영역에 따라 UV-A(장파장 자외선), UV-B(중파장 자외선)및 UV-C(단파장 자외선)으로 나뉜다. 이 중 인체에 가장 유해한 것으로 알려진 단파장의 UV-C는 파장이 짧아 침투력이 미약해 오존층과 성층권에서 대부분 흡수되므로 생물학적으로 큰 의미는 없다. 그러나 자외선 중 UV-B와 UV-A 영역의 자외선은 인체에 영향을 가장 많이 미치므로 이들 영역의 자외선을 차단하는 것이 필요하다. Vitamin C는 아스코르브산(ascorbic acid)이라고도 불리며 미백효과, 주름 개선, 자외선 차단 및 항산화 작용을 하지만 섬유가공 시 Vitamin C는 쉽게 산화되며 세탁 내구성이 떨어지기 때문에 가교제를 필요로 한다. Vitamin C를 이용하여 자외선 차단성 섬유제품을 개발하기 위해, 포름알데히드를 유리하지 않는 폴리카르복시산 가교제 중 하나인 Citric Acid(CA)를 사용하여 고온에서 면직물에 처리했을 때 에스테르 형 가교를 형성하여 내세탁성을 가질 수 있었다. 자외선 차단 효과를 알아보기 위하여 KS K 0850 섬유제품의 자외선 차단율 및 차단지수 시험방법으로 평가하였다.

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The Study on Bleaching of Kenaf Fibers (Part I) -Effect of Bleaching- (케냐프 섬유의 표백에 대한 연구 (제1보) -표백효과를 중심으로-)

  • Jang Hyunsook;Lee Hyeja;Yoo Hyeja;Han Youngsook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.9_10 s.146
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    • pp.1295-1305
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    • 2005
  • The study was based on a three-stage, non-repetitive factorial experiment in which chemical-rotted kenaf fibers were treated separately with hydrogen peroxide concentrations of $0.5\%,\;1\%\;and\;2\%$, with pH solutions of 7, 9 and 11, and treatment times of 30, 60 and 90 minutes. Under optimal conditions, the study was conducted to determine the bleaching efficiency by the addition of chelators, penetrants and surfactants. The bleaching effects on the kenaf fibers were high in high hydrogen peroxide concentration, high in alkali solution pH, low in long treatment time. The optimal level of hydrogen peroxide on bleaching effects were at $2\%$ hydrogen peroxide concentration, with pH of 11 and treatment time of 60 minutes. Under the conditions of $2\%$ hydrogen peroxide concentration, pH 11 and treatment time of 60 minutes, the addition of chelator: Sodim Pyrophosphate(SP), Citric Acid(CA) made the bleaching effects of the kenaf fibers high.

Metal Protease from Streptomyces spp. - I. Isolation of the Strain and the Enzymatic Properties - (Streptomyces 속균(屬菌)이 생성하는 Metal Protease - 제 1 보 : 균(菌)의 분리(分離) 및 효소학적(酵素學的) 성질(成質) -)

  • Yi, Dong-Heui;Yu, Choon-Bal
    • Korean Journal of Food Science and Technology
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    • v.12 no.1
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    • pp.13-17
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    • 1980
  • A Streptomyces spp. strain SY 79-1 which was capable of producing metal protease was isolated from soil. The optimal pH and temperature of the protease were around pH 8.0 and $45^{\circ}C$, respectively. The stable pH range of the enzyme was between pH 6.0 to 8.0. The enzyme was stable at $45^{\circ}C$, but it lost the activity about 75 % for 5 min and completely for 30 min when it was treated at $60^{\circ}C$. The activity of the enzyme was inhibited by $Hg^{++},\;Cu^{++},\;Ag^{+}$ and activated by $Mg^{++},\;Mn^{++},\;Co^{++},\;but\;Fe^{++},\;Ca^{++},\;Pb^{++}\;and\;Al^{3+}$ did not affect enzyme activity. This enzyme was strongly inhibited by EDTA, but was not inhibited by 2, 4-DNP, ${\rho}$-CMB, ${\varepsilon}$-aminocaproic acid, cysteine, thiourea, citric acid, oxalic acid and sodium arsenate. When cobalt was added to the EDTA-denatured enzyme, the activity of the enzyme was restored.

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Effect of Different Media on Growth and Yield in Hydroponic culture of Angelica keiskei Koidzumi (신선초(神仙草) 양액재배시(養夜載培時) 고형배지종류(固形培地種類)에 따른 생육(生育) 및 수량(收量))

  • Han, Seoung-Ho;Choi, Byung-Jun;Shin, Cheol-Woo;Chang, Ki-Woon;Han, Kwang-Seop;Choi, Kang-Ju;Woo, In-Sik;Park, Sang-Il
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.1
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    • pp.29-40
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    • 2000
  • This experiment was conducted to find appropriate media in hydroponic Culture of Angelica keiskei. The mediaused were carbonized rice hull, perlite, vermiculite, mixture(carbonized rice hull (1) : perlite (1) : peatmoss (1)), mixture+peatmoss20%, mixture+peatmoss40%, mixtrue+peatmoss60%, perlite 40%+peatmoss40%+vermiculite20% and open field cultivation. The highest yield of Angeli ca keiskei was shown 4,428ka/10a at vermiculite. The yield of vermiculite was increased about 133 percent compared to that of open field cultivation. The highest germanium contents was shown 0.52ppm at mixture+peatmoss 40%. The contents of germanium increased about 108 percent at mixture+peatmoss40% compared to that of open field cultivation.

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EFFECT OF FLUORIDE AND CALCIUM ON ENAMEL REMINERALIZATION IN VITRO (불소와 칼슘의 법랑질 재광화 효과에 대한 생체외 연구)

  • Lee, Kwang-Hee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.4
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    • pp.624-629
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    • 2004
  • The purpose of study was to observe the effect of fluoride and calcium on enamel remineralizaton in vitro. Human premolar enamel specimens were prepared by demineralization in $0.1{\sim}l.0%$ citric acid for 60 minutes. They were remineralized for 6 hours in one of the 1311owing solutions : (1) artificial saliva, (2) artificial saliva with 100ppmF, (3) artificial saliva with 1000ppmF, (4) artificial saliva with 1000ppmCa, and (5) artificial saliva with 100ppmF and 1000ppmCa. No significant remineralization was occurred in artificial saliva and artificial saliva with 100ppmF. Significant remineralization was observed in artificial saliva with 1000ppmF at 3 hours, and in artificial saliva with 1000ppmCa and artificial saliva with 100ppmF and 1000ppmCa at 3 and 6 hours(P<0.05). The remineralization effect of artificial saliva with 100ppmF and 1000ppmCa was greater than that of artificial saliva or artificial saliva with 100ppmF. Addition of F to 100ppm or 1000ppm, addition of Ca to 1000ppm, and increasing the concentration of F from 100ppm to 1000ppm did not significantly increase the remineralization.

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Preparation and Characterization of ClO2 Self-Releasing Smart Sachet (이산화염소 자체 방출 스마트 샤쉐의 제조 및 특성 연구)

  • Junseok Lee;Hojun Shin;Sadeghi Kambiz;Jongchul Seo
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.29 no.1
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    • pp.1-7
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    • 2023
  • Chlorine dioxide (ClO2) is widely used for post-harvest sterilization treatment. However, there are limitations in the retail application of ClO2 due to difficulties in handling, expensive facilities, and safety concerns. Therefore, it is necessary to develop a ClO2 technology that can be easily applied and continuously released for a long period. In this study, a series of ClO2 self-releasing sachets were developed. First, poly(ether-block-amide) (PEBAX) and polyethylene-glycol (PEG) composite films containing different ratios of citric acid (CA) were prepared using the solution casting method. The as-prepared PEBAX/PEG-CA composite films were evaluated using FT-IR, DSC, and TGA to confirm chemical structure and thermal properties. Subsequently, PEBAX/PEG-CA composite films were designed in the form of a sachet and NaClO2 powder was transferred into the sachet to achieve a ClO2 self-releasing system. The ClO2-releasing behavior of the sachet was investigated by measuring the release amount of the gas using UV-vis. The release amount of ClO2 increased with increasing CA contents owing to the existence of higher protons (trigger) in the polymer matrix. Further, ClO2 gas was released for a longer time. Therefore, the as-prepared smart sachet can be tuned according to applications and packaging sizes to serve an optimal sterilization effect.

Quality Characteristics of Soybean Curd Mixed with Freeze Dried Onion Powder (동결건조 양파분말을 첨가한 두부의 품질특성)

  • Kang, Nan-Suk;Kim, Jun-Han;Kim, Jong-Kuk
    • Food Science and Preservation
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    • v.14 no.1
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    • pp.47-53
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    • 2007
  • Soybean curd was mixed with onion powder to develop new foods, and changes in quality characteristics were investigated. The moisture content of onion soybean curd rose as the proportion of onion powder increased Whiteness (as measured by the L value) was high in soybean cud admixed with 0.1% (w/v) onion powder, Redness (the a value) was not significantly altered (the readings were 1.03-1.54) on addition of various onion powder concentrations. Yellowness (the b value) was similarly unaffected (readings 13.00-13.93) when various levels of onion powderwere added. Free sugar analysis showed that glucose was high in soybean curd (67.22 g/100 g) admixed with 0.1% (w/v) onion powder, The main organic acid was tartaric acid, and control organic acids included citric and oxalic acids at high levels. The major free amino acids were L-arginine, ${\gamma}-Amino-n-butyric$ acid, L-histidine, L-glutamic acid, L-serine, L-tyrosine and L-threonine, and amino acid content were high in soybean curd admixed with 0.2% (w/v) onion powder. Major phenolic compounds of onion soybean curd were quercitrin, protocatechuic acid and caffeic acid. The hardness of onion soybean curd was similar to that of the control when onion powder was added to 0.1% or 0.2% (w/v), and decreasedmore onion powder was added. Organoleptic qualities dropped as onion powder levels increased. In summary, onion powder addition to soybean curd is optimal at the 0.2% (w/v) level..

Development of Micronutrient Fertilizer -I. Characteristics of Raw Materials and Products (종합미량원소비료(綜合微量元素肥料)의 개발(開發)에 관(關)한 연구 -I. 원료(原料) 및 제품(製品)의 특성(特性))

  • Lim, Dong-Kyu;Seong, Ki-Seog;Shin, Jae-Sung;Park, Young-Sun
    • Korean Journal of Soil Science and Fertilizer
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    • v.14 no.2
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    • pp.88-94
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    • 1981
  • A trial manulacturing of the micronutrient fertilizer made of local mineral ores was conducted. A powdered mixture of raw materials was fused in an electric furnance and the molten matrix was quenched, dried and milled. Some informations from the manufacturing were summarized as followings. 1. Of all the ore minerals, only borax and manganese ores could be molten independently. 2. Borax was the best material for decreasing the molten temperature. 3. The molten temperature was increased with increased Ca and Mg content of the mixture. 4. The amounts of 2 % citric acid soluble Fe, Zn, and Mo were increased rapidly with increased temperature, but those of B and Mn were constant. 5. During the manufacturing process, the nutrient loss was high in order of boron, zinc and molybdenium.

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