• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.740-748
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• 2022

As circ_UBE2D2 has been confirmed to have targeted binding sites with multiple miRNAs involved in septic acute kidney injury (SAKI), efforts in this study are directed to unveiling the specific role and relevant mechanism of circ_UBE2D2 in SAKI. HK-2 cells were treated with lipopolysaccharide (LPS) to construct SAKI model in vitro. After sh-circ_UBE2D2 was transfected into cells, the transfection efficiency was detected by qRT-PCR, cell viability and apoptosis were determined by MTT assay and flow cytometry, and expressions of Bcl-2, Bax and Cleaved-caspase 3 were quantified by western blot. Target genes associated with circ_UBE2D2 were predicted using bioinformatics analysis. After the establishment of SAKI rat model, HE staining and TUNEL staining were exploited to observe the effect of circ_UBE2D2 on tissue damage and cell apoptosis. The expression of circ_UBE2D2 was overtly elevated in LPS-induced HK-2 cells. Sh-circ_UBE2D2 can offset the inhibition of cell viability and the promotion of cell apoptosis induced by LPS. Circ_UBE2D2 and miR-370-3p as well as miR-370-3p and NR4A3 have targeted binding sites. MiR-370-3p inhibitor reversed the promoting effect of circ_UB2D2 silencing on viability of LPS-treated cells, but shNR4A3 neutralized the above inhibitory effect of miR-370-3p inhibitor. MiR-370-3p inhibitor weakened the down-regulation of NR4A3, Bax and Cleaved caspase-3 and the up-regulation of Bcl-2 induced by circ_UB2D2 silencing, but these trends were reversed by shNR4A3. In addition, sh-circ_UBE2D2 could alleviate the damage of rat kidney tissue. Circ_UBE2D2 mitigates the progression of SAKI in rats by targeting miR-370-3p/NR4A3 axis.

• 콘크리트학회지
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• 제7권5호
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• pp.133-144
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• 1995

본 연구는 콘크리트 충전강관을 고층 건물의 구조부재로 이용하기 위한 연구의 일환으로서 강관의 폭두께비, 세장비와 충전콘크리트의 강도를 주요 변수로 하여 강관이 콘크리트를 단순 구속하는 경우의 재하조건으로서 일련의 실험을 콘크리트 충전강관 기둥의 역학적인 거동 특성을 고찰하였다. 얻어진 결론을 요약하면 다음과 같다. (1)구속 콘크리트의 파괴양상은 단주의 경우 시험체 단부에서의 압괴에 의한 $45^{\circ}$정도의 사인장 파괴가 이루어졌으며 장주의 경우 횡방향 휨 파괴 양상을 나타내었다. (2)원형강관으로서 콘크리트를 구속함으로서 변형능력의 향상과 동시에 콘크리트의 연성 효과를 증대시킬 수 있었다. (3)강관의 세장비, 폭두께비, 콘크리트의 강도를 고려하여 콘크리트의 구속계수를 이용하여 강관에 의해 구속된 내부 콘크리트와 충전 강관 기둥의 최대내력 산정식을 제안하였다.

• Journal of Ecology and Environment
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• 제41권3호
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• pp.45-53
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• 2017

Background: There have been many studies on the growth conditions of Zostera marina and Zostera japonica, but few studies have examined how spatial and temporal factors affect growth in established seagrass beds or the distribution range and shoot density. This study aims to clarify the factors that determine the temporal and spatial distribution of Zostera marina and Zostera japonica in the Seto Inland Sea east of Yamaguchi Prefecture. Methods: The study site is in Hiroshima Bay of the Seto Inland Sea, along the east coast of Yamaguchi Prefecture, Japan. We monitored by diving observation to confirm shoot density, presence or absence of both species and observed water temperature, salinity by sensor in study sites. Results: The frequency of occurrence of Zostera marina was high in all seasons, even in water depths of D.L. + 1 to -5 m ($80{\pm}34%$ to $89{\pm}19%$; mean ${\pm}$ standard deviation), but lower (as low as $43{\pm}34%$) near the breakwall, where datum level was 1 to 2 m, and it was further reduced in datum level -5 m and deeper. The frequency of occurrence of Zostera japonica was highest in water with a datum level of +1 to 0 m. However, in datum level of 0 m or deeper, it became lower as the water depth became deeper. Datum level +1 m to 0 m was an optimal water depth for both species. The frequency of occurrence and the shoot density of both species showed no negative correlation. In 2011, the daily mean water temperature was $10^{\circ}C$ or less on more days than in other years and the feeding damage by S. fuscescens in the study sites caused damage at the tips. Conclusions: We considered that the relationship between these species at the optimal water depth was not competitive, but due to differences in spatial distribution, Zostera marina and Zostera japonica do not influence each other due to temperature conditions and feeding damage and other environmental conditions. Zostera japonica required light intensity than Zostera marina, and the water depth played an important role in the distribution of both species.