• 농약과학회지
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• 제17권4호
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• pp.419-427
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• 2013

본 연구에서는 식물정유 124종과 6종의 분무제형(SF-0.25, 0.5, 1, 2.5, 5 및 10% sprays)을 대상으로 미국선녀벌레 약충과 성충에 대한 독성을 평가 하였다. 약충에 대한 실험은 1,000 mg/L와 500 mg/L로 실시 하였으며, 엽침지법을 사용하였다. 미국선녀벌레 약충에 대한 124종의 식물정유의 살충성을 스크리닝한 결과, 64종의 식물정유가 60% 이상의 사충률을 보였고, 이 중 19종의 식물정유는 100%의 사충률을 보였다. 일차 활성이 좋은 식물정유를 500 mg/L로 검정한 결과 cinnamon technical, cinnamon green leaf, cinnamon #500, cassia tree, citronella java 및 pennyroyal oil이 100%의 살충활성을 나타냈고, origanum, thyme white, grapefruit, savory, fennel sweet, aniseed 및 cinnamon bark oil 순으로 93.3%에서 80%까지 높은 살충활성을 보였다. Thyme red, tagetes, calamus, lemon eucalyptus, geranium oil은 73.3%에서 60% 정도의 살충활성을 보였다. 이중 100%의 높은 살충활성을 보인 정유 12종을 6농도의 분무제형으로 미국선녀벌레 성충에 대한 살충활성을 검정한 결과, cinnamon technical oil이 SF-0.5의 제형에서 100%의 살충활성을 보였으며, cinnamon #500, cinnamon green leaf, pennyroyal oil은 SF-2.5에서 100%의 살충활성을 보였다. 농업환경에서 고독성 합성살충제의 사용을 줄일 수 있는 방안으로 본 논문에서 선발한 식물정유가 미국선녀벌레의 약충 및 성충 방제에 유용한 수단으로 이용될 수 있을 것으로 생각된다.

• 한국잡초학회지
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• 제30권3호
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• pp.199-205
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• 2010

본 연구는 19종의 식물정유가 유채종자의 발아 및 생장에 미치는 영향을 파악하기 위하여 수행되었다. 그 결과 식물정유를 100배 희석 처리 한 경우 anise, cinnamon, citronella, clove, geranium, lemongrass, mustard 및 pine oil 등의 8종은 유채의 발아를 완전히 저해하였으며, 초기생장의 저해효과도 큰 것으로 나타났다. 유채의 발아와 초기생장에 대한 저해율을 근거로 하여, 상기의 8개의 식물정유로부터 cinnamon, clove 및 geranium oil이 실용 가능성이 있는 bio-herbicide로서 선발되었다. 선발된 cinnamon, clove 및 geranium oil을 토양처리한 경우 90kg ai $ha^{-1}$농도에서 유채의 출현율은 각각 7.1, 25.0, 3.6% 였으며, 초기생장율도 22.0, 9.9 및 11.0% 수준으로, 유채종자의 발아와 초기생장억제에 미치는 효과가 큰 것으로 나타났다.

• International Journal of Clinical Preventive Dentistry
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• 제14권4호
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• pp.216-221
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• 2018

Objective: In this study, we tried to investigate the antimicrobial activity of natural herbaceous plant-derived essential oils against oral disease-causing bacterial strains and establish the scientific evidences to apply them to the dentifrice, mouthwash, and other uses. Methods: We used the disk diffusion method with 23 types of 100% natural essential oils to verify the antimicrobial effect against Streptococcus mutans, Porphyromonas gingivalis, and Lactobacillus rhamnosus. After adhering to the surface of the plate culture medium, $20{\mu}l$ of the essential oil was prepared, and the diameter of the transparent ring was measured after incubation for 24 hours. All the experiments were repeated three times. Results: Among 23 types of oils, 13 were effective against all three strains: myrrh, ginger, basil, carrot seed, tea tree, patchouli, ylang ylang, cypress, lemongrass, cinnamon, peppermint, lavender, and eucalryptus. Seventeen oils were effective against S. mutans, and myrrh, basil, and carrot seed showed high antimicrobial activity. Eighteen oils were effective against P. gingivalis, and tea tree, carrot seed, and cinnamons showed high antimicrobial activity. Sixteen oils were effective against L. rhamnosus, and carrot seed and peppermint cinnamon showed high antimicrobial activity. Conclusion: Consequently, 13 essential oils showed the antimicrobial activity against three bacterial strains, which indicates these essential oils might be used as the effective materials to suppress the growth of oral-disease inducing microorganisms. Moreover, essential oils that have been analyzed activities in this study will be mixed up within the constant range and analyzed their antimicrobial effects to examine the synergistic activity among them.

• Asian-Australasian Journal of Animal Sciences
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• 제28권9호
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• pp.1281-1287
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• 2015

This study was performed to determine the effect of field pea silages which were the organic acid (OA) alternative of oregano and cinnamon essential oils on fermentation quality and aerobic stability. Whole crop pea was harvested at full pod stage and wilted in the laboratory at the 48 h. The chopped pea was mixed and divided into equal portions allocated to five groups: CON (non-treated), distilled water, denoted as control group; OA group, a mixture of 60% formic acid, 20% sodium formate and 20% water applied at a rate of 5 g/kg fresh forage (Silofarm Liquid, Farmavet); origanum (ORE) group, Origanum onites essential oil at 400 mg/kg fresh forage; cinnamon (CIN) group, cinnamon essential oil at 400 mg/kg fresh forage; origanum+cinnamon (ORECIN) group, a mixture of ORE and CIN applied at an equal rate of 400 mg/kg fresh forage. Cinnamon decreased acetic acid (AA), ammonia nitrogen ($NH_3-N$) and weight loss (WL) at the end of 60 days silage. Crude protein (CP) and dry matter (DM) increased by cinnamon essential oil. Yeasts were not detected in any treatments, including the control, after 7 days of air exposure. The $CO_2$ amount decreased and the formation mold was inhibited in the aerobic period by the addition of cinnamon oil. Oregano did not show a similar effect, but when it was used with cinnamon, it showed synergic effect on AA and during aerobic period, it showed antagonistic effect on mold formation and DM losses. It was found in this study that cinnamon can be an alternative to organic acids.

• The Plant Pathology Journal
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• 제33권6호
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• pp.582-588
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• 2017

The objective of this study was to determine inhibitory activities of four volatile plant essential oils (cinnamon oil, fennel oil, origanum oil and thyme oil) on in vitro growth of Fusarium oxysporum f. sp. fragariae causing Fusarium wilt of strawberry plants. Results showed that these essential oils inhibited in vitro conidial germination and mycelial growth of F. oxysporum f. sp. fragariae in a dose-dependent manner. Cinnamon oil was found to be most effective one in suppressing conidial germination while fennel oil, origanum oil and thyme oil showed moderate inhibition of conidial germination at similar levels. Cinnamon oil, origanum oil and thyme oil showed moderate antifungal activities against mycelial growth at similar levels while fennel oil had relatively lower antifungal activity against mycelial growth. Antifungal effects of these four plant essential oils in different combinations on in vitro fungal growth were also evaluated. These essential oils demonstrated synergistic antifungal activities against conidial germination and mycelial growth of F. oxysporum f. sp. fragariae in vitro. Simultaneous application of origanum oil and thyme oil enhanced their antimicrobial activities against conidial germination and fungal mycelial growth. These results underpin that volatile plant essential oils could be used in eco-friendly integrated disease management of Fusarium wilt in strawberry fields.

• 식물병연구
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• 제24권4호
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• pp.292-301
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• 2018

Alternaria leaf spot disease has been a concern during a tomato production in greenhouse. In vitro antifungal activities of vapours of four plant essential oils, cinnamon oil, fennel oil, origanum oil and thyme oil, were investigated during in vitro conidial germination and mycelial growth of Alternaria alternata causing the tomato leaf spots to find eco-friendly alternatives for chemical fungicides. The four plant essential oils showed different antifungal activities against in vitro conidial germination of A. alternata in dose-dependent manners, and cinnamon oil vapour was most effective to suppress the conidial germination. The four plant essential oils showed similar antifungal activities against the in vitro mycelial growth of A. alternata in dose-dependent manners, but low doses of thyme oil vapour slightly increased in vitro mycelial growth of A. alternata. Necrotic lesions on the A. alternata-inoculated tomato leaves were reduced differently depending on kinds and concentrations of plant essential oils. Delayed conidial germination and germ-tube elongation of A. alternata were found on the tomato leaves treated with cinnamon oil and origanum oil vapours at 6 hpi. These results suggest that volatiles from cinnamon oil and origanum oil can be provided as alternatives to manage Alternaria leaf spot during the tomato production eco-friendly.

• International Journal of Industrial Entomology and Biomaterials
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• 제32권1호
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• pp.26-34
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• 2016

The present study was developed in order to evaluate the effect of five essential oils on the workers of the leaf-cutting ants; Atta sexdens rubropilosa and Acromyrmex subterraneus molestans by contact with a treated surface and ingestion with a treated leaves.. The essential oils of cinnamon, clove and mustard had generally more effective with 5, 10 and 15% concentrations after 24, 48, 72 and 96 h against workers of A. sexdens rubropilosa and A. subterraneus molestans in contact bioassay, but mustard was the most effective in ingestion bioassay on both species. On the other hand, there was no significant difference among the essential oils with 1% concentration and control after 24, 48 and 72 h of treatment in contact and ingestion bioassays against workers of A. sexdens rubropilosa . However, Andiroba oil had less efficiency values in all concentrations been used. Therefore, the essential oils of mustard, cinnamon and clove have contact and ingestion effects on workers of A. sexdens rubropilosa and A. subterraneus molestans, and may be promising on the leaf-cutting ant control.

• 한국식품위생안전성학회지
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• 제36권5호
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• pp.430-439
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• 2021

항균활성이 뛰어난 식물 정유를 이용하여 식품가공 기구 및 용기에 다양하게 사용되는 polyethylene과 stainless steel 표면에 형성된 식중독 6종의 biofilm 형성에 대한 억제 효과를 연구하였다. 식물 정유 20종의 식중독 미생물에 대한 항균활성을 disk diffusion 방법으로 평가한 결과 cinnamon > clove > lemongrass > peppermint > pine needle 순으로 항균활성을 나타냈다. Cinnamon과 clove 정유의 최소억제농도(MIC)와 최소살균농도(MBC)는 각각 0.63-1.25 mg/mL과 1.25-2. mg/mL의 범위를 나타냈으며, lemongrass 정유의 MIC와 MBC는 각각 1.25-2.50 mg/mL과 2.50-5.00 mg/mL로 약간 낮은 항균활성을 나타냈다. 정유 3종의 biofilm 형성 예방효과는 식중독 미생물과 polyethylene 및 stainless steel에 따라 다소 차이가 있었지만, 0.5% 농도의 cinnamon, clove와 lemongrass 정유를 식품접촉 표면에 미리 코팅하였을 때 biofilm 형성에 영향을 주는 것을 확인하였다. 정유의 농도가 증가할수록 모든 식중독 미생물에 대해서 biofilm 형성을 유의적으로 억제하였으며(P<0.05), 0.5% cinnamon과 clove 정유 처리에 의해 L. monocytogenes ATCC 19112와 S. aureus KCCM 11812의 biofilm이 형성되지 않았다. Polyethylene과 stainless steel coupon 표면에 형성된 식중독 미생물의 biofilm의 제거 효과를 측정한 결과, 식중독 미생물의 종류에 따라 차이가 있었지만 정유의 농도가 증가할수록 biofilm 제거 효과는 높아졌으며, 대체로 clove 정유의 biofilm 제거율이 높은 것으로 나타났다. 본 연구를 통하여 0.5%의 cinnamon과 clove 정유는 polyethylene과 stainless steel 표면에 식중독 미생물이 형성하는 biofilm을 예방, 성장 억제 및 제거할 수 있는 천연 소재로 적용이 가능한 것으로 나타났다.

• The Plant Pathology Journal
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• 제31권3호
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• pp.269-277
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• 2015

Anthracnose caused by Colletotrichum gloeosporioides has been destructive during pepper fruit production in outdoor fields in Korea. In vitro antifungal activities of 15 different plant essential oils or its components were evaluated during conidial germination and mycelial growth of C. gloeosporioides. In vitro conidial germination was most drastically inhibited by vapour treatments with carvacrol, cinnamon oil, trans-cinnamaldehyde, citral, p-cymene and linalool. Inhibition of the mycelial growth by indirect vapour treatment with essential oils was also demonstrated compared with untreated control. Carvacrol, cinnamon oil, trans-cinnamaldehyde, citral and eugenol were among the most inhibitory plant essential oils by the indirect antifungal efficacies. Plant protection efficacies of the plant essential oils were demonstrated by reduced lesion diameter on the C. gloeosporioides-inoculated immature green pepper fruits compared to the inoculated control fruits without any plant essential oil treatment. In planta test showed that all plant essential oils tested in this study demonstrated plant protection efficacies against pepper fruit anthracnose with similar levels. Thus, application of different plant essential oils can be used for ecofriendly disease management of anthracnose during pepper fruit production.

• 한국유기농업학회지
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• 제26권2호
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• pp.259-268
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• 2018

The study investigated the efficacy of four different essential oils on the repellent responses of large black chafer (Holotrichia parallela) Motschulsky (Coleoptera: Scarabaeidae) in organic pear (Pyrus pyrifolia) orchards. Cinnamon, pine, peppermint, and eucalyptus oils were used, and the behavioral responses and repellent effects against H. parallela were investigated under laboratory and field conditions. Adult beetle responses to different oils were examined using a Y-tube olfactometer in the laboratory and four absorbent blocks with each oil in the field. The repellent responses rates of H. parallela were 100% for cinnamon oil; however, only 67% of adult beetles avoided peppermint and eucalyptus oil in the Y-tube olfactometer bioassay. In the field tests, the least damage to leaves was observed on trees treated with cinnamon oil, whereas the most damage was observed in the control (non-treated) trees and those treated with peppermint oil, followed by eucalyptus and pine oil. Therefore, cinnamon oil can be used as a repellent to avoid damage form large black chafers in organic pear orchards.