• Natural Product Sciences
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• v.14 no.2
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• pp.138-142
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• 2008

The composition of essential oils from Chrysanthemum zawadskii var. latilobum and C. indicum were analyzed and compared. The results of gas chromatography-mass spectrometry revealed there were distinctly different compositional patterns between C. zawadskii var. latilobum and C. indicum essential oils. The combinatorial effect of the oil of C. zawadskii var. latilobum and C. indicum, with various antibiotics was assessed against antibiotic-susceptible and -resistant strains of Staphylococcus aureus and Streptococcus pneumoniae. The essential oil fraction significantly inhibited most of the tested antibiotic-susceptible and -resistant strains of S. pneumoniae, with minimum inhibiting concentrations (MICs) ranging from 0.5 to 4.0 mg/ml. The fractional inhibiting concentration indices (FICIs) of the oils when combined with antibiotics against S. aureus and S. pneumoniae ranged from 0.26 to 0.75, and showed synergistic or additive effects.

• Preventive Nutrition and Food Science
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• v.17 no.3
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• pp.234-238
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• 2012

The volatile aroma constituents of Chrysanthemum zawadskii var. latilobum K. were separated by hydro distillation extraction (HDE) method using a Clevenger-type apparatus, and analyzed by gas chromatography-mass spectrometry (GC/MS). The yield of C. zawadskii var. latilobum K. flower essential oil (FEO) was 0.12% (w/w) and the color was light green. Fifty-five volatile chemical components, which make up 88.38% of the total aroma composition, were tentatively characterized. C. zawadskii var. latilobum K. FEOs contained 27 hydrocarbons, 12 alcohols, 7 ketones, 4 esters, 1 aldehyde, 1 amine, and 3 miscellaneous components. The major functional groups were terpene alcohol and ketone. Borneol (12.96), (${\pm}$)-7-epi-amiteol (12.60), and camphor (10.54%) were the predominant volatiles. These compounds can be used in food and pharmaceutical industries due to their active bio-functional properties.

• YAKHAK HOEJI
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• v.45 no.6
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• pp.604-610
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• 2001

Chrysanthemum zawadskii var. latilobum (Compositae), which is called Gu-Jul-Cho, has been used as traditional medicine in pneumonia, bronchitis, common cold, pharyngitis, bladder-related disorders, women's diseases, and hypertension, etc. In this study we have investigated various biological activities of methanol extracts and their main component, linarin isolated from this herb. The antipyretic effect of them was tested by Brewers yeast method and the antiinflammatory activities, with arachidonic acid, o-tet-radecanoylphorbol 13-acetate and carrageenan. Their antipyretic and antiinflammatory activities were more potent than acetaminophene and aspirin. They also showed stronger hepatoprotective activities than ones of silymarin in mice.

• Journal of Plant Biology
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• v.10 no.1_2
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• pp.31-35
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• 1967

This study was made on three taxa of Chrysanthemum that grew on the Ewha Womans University campus on the basis of gross morphology, pollen epidermal patterns and chromosomes. The three taxa were recognized as Chrysanthemum zawadskii subsp. zawadskii var. acutilobum(2n=54); Chrysanthemum zawadskii subsp. latilobum (2n=36), and possible hybrid(2n=45) between the two taxa in view of leaf morphorlogy and chromosome number counts.

• The Korean Journal of Food And Nutrition
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• v.31 no.3
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• pp.378-387
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• 2018

This study investigated the volatile flavor composition of essential oils from Chrysanthemum zawadskii var. latilobum Kitamura and Aster yomena Makino. The essential oils obtained by the hydrodistillation extraction method from the aerial parts of the plants were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). One hundred (95.04%) volatile flavor compounds were identified in the essential oil from the C. zawadskii var. latilobum Kitamura. The major compounds were valencene (10.82%), ${\delta}$-cadinol (9.77%), hexadecanoic acid (8.70%), 2-methyl-4-(2,6,6-trimethylcyclohex-1-enyl) but-2-en-1-ol (3.67%), and 2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4]non-3-ene (3.57%). Ninety-eight (93.83%) volatile flavor compounds were identified in the essential oil from the Aster yomena Makino. The major compounds were and 3-eicosyne (13.61%), 9,10,12-octadecatrienoic acid (7.8%), ${\alpha}$-caryophyllene alcohol (6.83%), 9-octadecynoic acid (6.03%), and ${\alpha}$-caryophyllene (5.74%). Although the two plants are apparently very similar, the chemical composition of the essential oils was significantly different in quality and quantity. In the case of C. zawadskii var. latilobum Kitamura, the sesquiterpene, valencene was found to be 10.82%, but it was not identified in A. yomena Makino. ${\delta}$-Cadinol appeared higher in C. zawadskii var. latilobum Kitamura than in A. yomena Makino. A clear characteristic of A. yomena Makino essential oil is that it has a high content of caryophyllene derivatives. The ${\alpha}$-caryophyllene alcohol contained in A. yomena Makino was relatively high at 6.83%, although the compound was not identified in C. zawadskii var. latilobum Kitamura. Also ${\alpha}$-caryophyllene was shown to be higher in A. yomena Makino than in C. zawadskii var. latilobum Kitamura.

• The Korean Journal of Food And Nutrition
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• v.29 no.3
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• pp.431-437
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• 2016

Macrophages play a pivotal role in the innate and adaptive immune systems. This study investigated the immuno-modulatory activities of polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) in macrophages. Polysaccharides from Chrysanthemum zawadskii var. latilobum were extracted by the ethanol precipitation method. RAW 264.7 mouse macrophage cell line was treated with CZPS (4 to $128{\mu}g/mL$), and there was no cytotoxicity at a dose below $32{\mu}g/mL$. The levels of nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-6, IL-$1{\beta}$) production in the CZPS treated group ($32{\mu}g/mL$) were $6.5{\pm}0.12{\mu}m$ (NO), $1252.8{\pm}79.85$ (TNF-${\alpha}$), $305.4{\pm}29.41$ (IL-6), and $683.3{\pm}59.71$ (IL-$1{\beta}$), respectively, and they were significantly increased when compared to the control group; $2.2{\pm}0.03{\mu}m$ (NO), $452.3{\pm}38.34$ (TNF-${\alpha}$), $31.7{\pm}5.75$ (IL-6), and $184.1{\pm}11.52$ (IL-$1{\beta}$). Additionally, protein expression of inducible nitric oxide synthase (iNOS) and phosphorylation of MAPKs and NF-${\kappa}B$ expression were significantly increased upon CZPS treatment. Therefore, these results indicated that polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) may have a potential immunomodulatory activity in macrophages through MAPKs and NF-${\kappa}B$ signaling, and this information is useful for the development of immune enhancing adjuvant materials using a natural ingredient.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.6
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• pp.86-94
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• 2006

This study was to analyze vegetation and soil characteristic, and ordination of Bupleurum euphorbioides community located in Mt. Seorak area, Mt. Sobaek area, and Mt. South Deogyu area. Bupleurum euphorbioides community was classified into Carex lanceolata subcommunity and Chrysanthemum zawadskii var. alpinum subcommunity, and Chrysanthemum zawadskii var. alpinum subcommunity was classified into Calamagrostis arundinacea and Carex breviculmis group. In the study sites, soil organic matter, nitrogen, available phosphorous, changeable potassium, changeable calcium, changeable magnesium concentration, cation exchange capacity, and soil pH were ranged from 9.0~15.2%, 0.32~0.47%, 3.0~8.6ppm, 0.3~0.9me/100g, 0.5~1.5me/100g, 0.2~0.6me/100g, 11.4~16.1me/100g, and 4.3~4.6, respectively. And, soil texture was sandy loam or loam. The Carex breviculmis group of Chrysanthemum zawadskii var. alpinum subcommunity was found in area of high elevation and had high exchangeable K, Ca, and Mg concentration. The Calamagrostis arundinacea group of Chrysanthemum zawadskii var.alpinum subcommunity was found in area of low elevation and had lower exchangeable K, Ca, and Mg concentration than in the other subcommunity. The Carex lanceolata subcommunity was found in area of medium elevation and had medium exchangeable K, Ca, and Mg concentration. The Carex lanceolata subcommunity was located in a path of top the mountain peak, and has been destroyed. We need any action to protect the subcommunity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.6
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• pp.782-788
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• 2013

Chrysanthemum zawadskii Herbich var. latilobum Kitamura (Compositae), colloquially known "Gujulcho" in Korea, has been used in traditional medicine for the treatment of various diseases, including cough, common cold, bladder-related disorders, gastroenteric disorders, hypertension, and inflammatory diseases, such as pneumonia, bronchitis, pharyngitis, and rheumatoid arthritis (RA) However, the effect of Chrysanthemum zawadskii var. latilobum on breast cancer invasion is unknown. In this study, we investigated the inhibitory effect of Chrysanthemum zawadskii var. latilobum extract (CZE) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced matrix metalloproteinase-9 (MMP-9) expression and cell invasion, as well as the molecular mechanisms involved in MCF-7 cells. CZE were not cytotoxic up to 100 ${\mu}g/ml$ concentration in the MCF-7 cell line. CZE decreased MMP-9 expression. TPA substantially increased NF-${\kappa}B$ DNA binding activity. Pre-treatment with CZE inhibited TPA-stimulated NF-${\kappa}B$ binding activity and NF-${\kappa}B$ related protein expression. To identify invasion ability of MCF-7 cells decreased by CZE, we used martrigel invasion assay. As a result, it is significantly decreased cell invasion. These results indicate that CZE-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the NF-${\kappa}B$ pathway in MCF-7 cells. Chrysanthemum zawadskii var. latilobum may have potential value in restricting breast cancer metastasis.

• Research in Plant Disease
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• v.17 no.2
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• pp.225-227
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• 2011

The stem rot of Chrysanthemum zawadskii var. latilobum occurred in the landscape field of Sanggwan-myeon, Wanju-gun, Jeonbuk, Korea in September 2009. The typical symptoms of the disease were water-soaking and dark brown rot on the stem, wilting and blighting. Most infected plants died of the disease. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were spherical or irregular, 0.8-3.5 (mean 1.8) mm in size on potato dextrose agar, brown in color. The optimal temperature range of hyphal growth was $25-30^{\circ}C$ and the hyphal diameter was 4-9 ${\mu}m$. Clamp connections were observed in the hyphae of the fungus grown on potato dextrose agar. Pathogenicity of the casual organism was provided on C. zawadskii according to Koch's postulate. The fungus was identified as Sclerotium rolfsii on the basis of mycologiclal characteristics and pathogenicity test to host plants. This is the first report on the stem rot of C. zawadskii caused by S. rolfsii in Korea.

• Journal of Microbiology and Biotechnology
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• v.22 no.5
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• pp.622-627
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• 2012

Mast cells and basophils are important effector cells in immunoglobulin-E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-methoxyluteolin, isolated from Chrysanthemum zawadskii, in the $Fc{\varepsilon}RI$-mediated allergic reaction. We determined that 6-methoxyluteolin inhibited anti-$Fc{\varepsilon}RI$ ${\alpha}$ chain antibody (CRA-1)-induced histamine release, as well as elevation of intracellular calcium concentration $[Ca^{2+}]_i$ in a dose-dependent manner. Moreover, the inhibitory effects of 6-methoxyluteolin on the cell surface expression and the mRNA level of the $Fc{\varepsilon}RI$ ${\alpha}$ chain were determined by flow cytometric analysis and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Therefore, these results show that 6-methoxyluteolin is a potent inhibitor of histamine release and calcium influx via down-regulation of the $Fc{\varepsilon}RI$ ${\alpha}$ chain.