• Proceedings of the Korea Society of Poultry Science Conference
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• 2000.11a
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• pp.85-87
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• 2000

To obtain the genetic information of Korean native livestock, the karyotyping of Korean native chick were performed by high-resolution banding technique. The chromosomes were prepared from lymphocyte culture and early embryos with 200 Korean native chick which have been raised at National Livestock Research Institute. There were no significant difference between Korean native chick and Leghorn in the number of chromosomes and chromosomal morphological pattern. Using high resolution banding technique, the yield of G-bands of prophase is much greater than that can be obtained by International System for Standardzed Avian Karyotypes(ISSAK, 1999). The G-band landmarks of Korean native chick were similar to those of ISSAK and Leghorn except some macrochromosomes. chromosome Z and 3 had C-band variants with heteromorphic patterns on distal and centromeric site. The proportion of constitutive heterochromatin, the heterochromatin ratio of Korean native chick was significantly more than that of Leghorn in all chromosomes.

• Journal of Plant Biology
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• v.15 no.1
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• pp.28-32
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• 1972

Young haploid leaf derived from the anthers of tobacco plant was cultuerd and plantlets of various ploidies were obtained. When the leaf was put on the medium supplemented with kinetin as growth regulator, plantlets developed directly from the leaf, and the plants coming out in early stage of culture were all haploid. Plants developing in later stage were mostly haploids with some exception of diploid and aneuploid. Leaves were also cultured on the callus-inducing media supplemented with 2,4-D and kinetiion, and the calluses were sub-cultured for six months. Plants developed from these calluses were mostly aneuploids of various chromosome numbers. In view of the fact that the plants directly developed from the leaf were all haploid, the tissue of the original leaf explant was assumed to be uniform as far as chromosome number was concerned. On the other hand, it seemed that the occurrence of various ploidies in the plants derived from the calluses of same origin was the result of the influence of in vitro culture. Apical meristem tissues and various multicellular bodies were formed in the epidermal and inner mesophyll tissues as well as in the sub-epidermal cells.

• Animal cells and systems
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• v.10 no.2
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• pp.65-71
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• 2006

Autosomal Dominant Polycystic Kidney Disease (ADPKD) is one of the most common inherited renal disorders in the world. Mutations in PKD1 located on chromosome 16p13.3 are responsible for 85% of all the ADPKD patients whereas mutations in PKD2 on chromosome 4q21-23 are responsible for the rest of the cases. Genetic heterogeneity and the problems of mutation detection in PKD1 suggest that linkage analysis is an important approach to study the genetics of ADPKD. To evaluate the availability of six (CA)n microsatellite markers for the linkage analysis of ADPKD in the Korean population, we examined the allele frequencies and heterozygosities of the markers. With the exception of KG8, five markers were highly informative, with PIC values over 0.5, but the PIC value of KG8 marker was less informative than other five markers because of the low number of alleles. Therefore, this study will be useful in linkage analysis for ADPKD families in the Korean population.

• Journal of Plant Biology
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• v.12 no.1
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• pp.35-48
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• 1969

This study was made on the taxa Chrysanthemum zawadskii complex that grow in South Korea on the basis of chromosomes, epidermis, pollens and gross morphology. I have found four types of chromosome numbers, 36, 45, 54, and 72 as a polyploidal series. Even though the gross morphology was quite similar almost the same gross morphology, chromosome number was different among the taxa. The taxa of 36 chromosomes present broad and fine lobed leaves which grow separately, broad leafed taxon in the mainland of Korea and the other's in Ullungdo Island which is isolated form the mainland in the East Sea. The taxa of 54 chromosomes are also present in the broad and in the fine lobed leaves. The fine lobed leave taxon grows in central to northern Korea and in the high altitude of mountains. Broad leafed taxon grows in central to southern Korea and comparatively lower altitude of the mountains. The taxon of 72 chromosomes is grown in the high altitude of Mt. Hallasan which is isolated from the mainland of Korea. According to this study of Chrysanthemum zawadskii complex, I have arranged the scientific names, as Chrysanthemum zawadskii subsp. latilobum, subsp. acutilobum, subsp. naktongenese, subsp. lucidum, subsp. coreanum and hybrid between subsp. acutilobum X subsp. latilobum.

• The Korean Journal of Zoology
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• v.16 no.3
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• pp.171-176
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• 1973

Metaphases were obtained from the bone marrow cells of B. kangii Yoon, by means of direct air-drying technique. The karylogical characteristics of this species were as follows; 1) The diploid chromosome number was 22(2n=22) which might be divided into six large and five small homololgous chromosmes. 2) All homologous chromosomes of this species were metacentrics. 3) The secondary constriction was not found in all members of chromosomes. 4) There was no evidence for the existence of a specific sex chromosome pair in this species.

• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.245-251
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• 1998

Test plants with 10 days old primary leaves were indouclated by shaking infected seedlings with sporulating colonies over them in an inoculation room under the conditions of 20$\pm$1 $^{\circ}C$ with constant illumination of 2.500 lux and 100% realtive humidity. A seeding reaction of 4 days after inoculation appreared in the trisomic types as opposed to Tri-5B line had been symtoms of a fungus 3 days after inoculation. The infection types of 8 days after inoculation were recognized with higher susceptibility to each trisomics in A genomie than B-genome. Tri-2A line showed less condium and there appeared symptoms of a conditions of mottle and formed papilla, and haustorium was not formed. However, Tri-5B line had much condium one overall leaves and showed a symtom like necrosis compared with normal plant. Moreover, Tri-5B line showed high sensitivity and high germination number of condium. These results inferred that resistant gene located on 2A chromosome and susceptibility gene is located on the chromosome 5B.

• Journal of Interdisciplinary Genomics
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• v.5 no.2
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• pp.13-17
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• 2023

KBG syndrome (KBGS) is a multisystem disorder characterized by short stature, distinctive facial features including macrodontia of upper central permanent incisors, and developmental/cognitive delay. It is caused by variants or deletion of Ankyrin Repeat Domain 11 (ANKRD11) located in chromosome 16q24.3. Since its initial report in 1975, KBG syndrome has been recognized as an exceedingly rare disorder. However, recent advancements in genetic diagnostic techniques have led to an increase in both the diagnosis rate and the number of reported cases, contributing to a rapid increase in its global prevalence. We review the clinical aspects of KBGS, including previously reported and newly reported cases, as well as the related genetic patterns discovered so far.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.235-243
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• 2002

This study was designed to examine the ability of the bovine (MII) oocytes cytoplasm to support several mitotic cell cycles under the direction of differentiated somatic cell nuclei of bovine, porcine, mouse and human. Bovine GV oocytes were matured in TCM-199 supplemented with 10% FBS. At 20h after IVM, recipient oocytes were stained with 5 $\mu\textrm{g}$/$m\ell$ Hoechst and their 1st polar body (PB) and MII plate were removed by enucleation micropipette under UV filter. Ear skin samples were obtained by biopsy from an adult bovine, porcine, mouse and human and cultured in 10% FBS added DMEM. Individual fibroblast was anlaysed chromosome number to confirm the specificity of species. Nuclear transferred (NT) units were produced by electrofusion of enucleated bovine oocytes with individual fibroblast. The reconstructed embryos were activated in 5 $\mu$M ionomycin for 5 min followed by 1.9 mM 6-dimethylaminopurine (DMAP) in CR1aa for 3 h. And cleaved NT embryos were cultured in CR1aa medium containing 10% FBS on monolayer of bovine cumulus cell for 8 days. Also NT embryo of 4~8 cell stage was analysed chromosome number to confirm the origin of nuclear transferred somatic cell. The rates of fusion between bovine recipient oocytes and bovine, porcine, mouse and human somatic cells were 70.2%, 70.2%, 72.4% and 63.0%, respectively. Also, their cleavage rates were 60.6%, 63.7%, 54.1% and 62.7%, respectively, there were no differences among them. in vitro development rates into morula and blastocyst were 17.5% and 4.3% in NT embryos from bovine and human fibroblasts, respectively. But NT embryos from porcine and mouse fibroblasts were blocked at 16~32-cell stage. The chromosome number in NT embryos from individual fibroblast was the same as chromosome number of individual species. These results show that bovine MII oocytes cytoplasm has the ability to support several mitotic cell cycles directed by newly introduced nuclear DNA.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.408-412
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• 1997

The cytogenetic analysis of river puffer, Takifugu obscurus belongs to Family Tetraodontidae, was performed. The chromosome number of T. obscurus was 44 and the fundamental number was 64. Heteromorphic sex chromosomes were not found. The mean cellular size and nuclear size were $11.01\times7.95{\mu}m$ and $4.05\times3.15{\mu}m$, respectively. The mean surface area and volume in cell and nucleus were $68.76{\mu}m^2\;and\;366.00{\mu}m^3,\;10.06{\mu}m^2\;and\;21.36{\mu}m^3$, respectively. The number of erythrocyte of both female and male was $12\~13\times10^5/m\ell$. Gill tissues from diploid individuals had cells with one or two nucleoli. These cytogenetic studies should be used for cytotaxonomy and as a valuable estimation of polyploidy to come in T. obscurus.

• Clinical and Experimental Reproductive Medicine
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• v.34 no.1
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• pp.41-48
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• 2007

Objective: To determine whether the presence of Y-chromosome microdeletion affects the outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) program. Methods: Fourteen couples with microdeletion in azoospermic factor (AZF)c region who attempted IVF/ICSI or cryopreserved and thawed embryo transfer cycles were enrolled. All of the men showed severe oligoasthenoteratoazoospermia (OATS) or azoospermia. As a control, 12 couples with OATS or azoospermia and having normal Y-chromosome were included. Both groups were divided into two subgroups by sperm source used in ICSI such as those who underwent testicular sperm extraction (TESE) and those used ejaculate sperm. We retrospectively analyzed our database in respect to the IVF outcomes. The outcome measures were mean number of good quality embryos, fertilization rates, implantation rates, $\beta$-hCG positive rates, early pregnancy loss and live birth rates. Results: Mean number of good quality embryos, implantation rates, $\beta$-hCG positive rates, early pregnancy loss rates and live birth rates were not significantly different between Y-chromosome microdeletion and control groups. But, fertilization rates in the Y-chromosome microdeletion group (61.1%) was significantly lower than that of control group (79.8%, p=0.003). Also, the subgroup underwent TESE and having AZFc microdeletion showed significantly lower fertilization rates (52.9%) than the subgroup underwent TESE and having normal Y-chromosome (79.5%, p=0.008). Otherwise, in the subgroups used ejaculate sperm, fertilization rates were showed tendency toward lower in couples having Y-chromosome microdeletion than couples with normal Y-chromosome. (65.5% versus 79.9%, p=0.082). But, there was no significance statistically. Conclusions: In IVF/ICSI cycles using TESE sperm, presence of V-chromosome microdeletion may adversely affect to fertilization ability of injected sperm. But, in cases of ejaculate sperm available for ICSI, IVF outcome was not affected by presence of Y-chromosome AZFc microdeletion. However, more larger scaled prospective study was needed to support our results.