• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.311-315
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• 2003

This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macrocephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A. japonica and A. macrocephala were 2n=24. The length. of the mitotic metaphase chromosomes of A. japonica ranged from $0.70\;to\;1.60{\mu}m$ with a total length. of $12.11{\mu}m$ and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A. macrocephala ranged from $0.90\;to\;2.35{\mu}m$ with a total length of $16.58{\mu}m$ and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A. japonica chromosomes was shorter than that of A. macrocephala, but A. japonica had one subtelocentrics (chromosomes 4) different from A. macrocepha1a. chromosomes. The F1SH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A. macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.

• Journal of Embryo Transfer
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• v.22 no.2
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• pp.137-141
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• 2007

Antioxidants were well known to be essential supplements in the complex media and serve as a reservoir of oxygen. In this study, Hanwoo COCs (cumulus oocytes complexes) were matured and developed in L-cysteine-TCM199 and analyzed metaphase chromosome. Maturation rate of Hanwoo COCs were 73.4%, 94.6% in 0.1% PVA, 0.1 mM L-cysteine, respectively and showed significantly different between the treatments (p<0.05). Blastocyst formation were revealed 20.3%, 10.0% in 5% FBS+TCM199, 0.1 mM L-cysteine+1% BSA, respectively. There were no significant difference among treatment groups. Metaphase chromosome were showed 18.3%, 12.0% in 5% FBS-TCM199, 0.1 mM L-cysteine, respectively and analyzable chromosome were 6.1%, 4.0% and had no differences between the treated groups. In the case of in vitro developmental stages, metaphase chromosome were showed 18.3%, 12.0% in $4{\sim}16$ cells stage, 43.1%, 13.0% in morulae stage and 94.8%, 100.0% in blastocyst stage. These results suggested L-cysteine has beneficial role for in virto maturation and development in Hanwoo COCs.

• Journal of Radiation Protection and Research
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• v.30 no.4
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• pp.209-219
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• 2005

This study was carried out to examine the effect of the DNA repair inhibitors, Cytosine Arabinoside(Ara C), 3-Aminobenzamide(3AB) and Hydroxyurea(HU) on the frequencies of radiation-induced micronuclei(MNi) and aneuploidy. Irradiated lymphocytes(1-3Gy) were treated with DNA repair inhibitors, Ara C, 3AB and HU for 3 hours and CBMN assay - FISH technique with DNA probe for chromosome 1 and 4 was performed. The frequencies of x-ray induced MNi and aneuploidy of chromosome 1 and 4 were increased in a dose-dependent manner. Ara C, 3AB and HU enhanced the frequencies of radiation-induced MNi and the frequencies of radiation-induced aneuploidy of chromosome 1 and 4 were enhanced by HU and Ara C while no effect was observed by 3AB. The frequency of radiation-induced aneuploidy of chromosome 1 was higher than that of chromosome 4. These results suggest that there are different mechanisms involved in the formation of MNi and aneuploidy by radiation.

• Parasites, Hosts and Diseases
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• v.21 no.1
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• pp.118-124
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• 1983

The distribution, external morphology, radula, chromosome numbers of Planorbidae snails were studied. 1. The specimens were collected at four stations in Nonsangun, Kongjugun, and Daedukgun which are located around Geum river. Three genera and three species of Planorbidae, Hippeutis cantorir Segmentina hemisphaerula and Gyraulus cenvexiusculus, were collected. H. cantori was the most abundant species among the three species. G. convexiusculus was the least abundant one. 2. Each species could be identified on the basis of its external characteristic, since the periphery of each species has a peculiar shape. H. cantori was the largest one among the three species. 3. The radula formula of each species was very similar to other species. The size of radula was proportional to the size of shell. The radula formulae of H. cantori, S. hemisphaerula, and G. convexiusculus were 29 : 1 : 29, 23 : 1 : 23, and 16 : 1 : 16 respectively. The difference of radula formula could be found in the total numbers of laternal and marginal teeth. 4. The haploid chromosome number of H. cantori was eighteen (n=18), S. hemisphaerula and G. convesiusculus were assumed to be same in their chromosome numbers (n: 18).

• Journal of Plant Biotechnology
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• v.3 no.1
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• pp.33-38
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• 2001

Protoplasts isolated from inbred lines of Brassica oleracea L. var capitata (cabbage) and Brassica campestris L. ssp. pekinensis (Chinese cabbage) were fused by PEG-mediated method, and somatic hybrid cells were differentiated into plants. for the identification of somatic hybrid plants, ploidy level, plant morphology, and cytological analysis were performed. All of the regenerated plants derived from fused protoplasts were shown to be 2X-4X, or higher ploidy level, presumably due to somatic hybridization or chromosome doubling. The morphology of leaves, petioles, and flowers showed an intermediate phenotype between Chinese cabbage and cabbage. Chromosome numbers in these somatic hybrids ranged mostly from 33 to 38. According to Genomic in situ hybridization (GISH) pattern, signals from both fusion parents of B.campestris or B.oleracea were detected in different colors when chromosomes of putative somatic hybrids were observed.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.2
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• pp.171-174
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• 1995

Heteromorphisms of chromosome banding patterns can be useful markers for gene mapping and other kinds of genetic studies. In Japanese quail, the centromere region of chromosome No. 4 is the site of a heteromorphism. One form of the C-band at this region is relatively small ("a" form); an alternative form is much larger ("b" form). To identify the transmission patterns, all possible matings were made between birds with karyotype a/a, a/b, and b/b. The outcome from all crosses are entirely consistent with the expectation from simple Mendelian transmission. No evidence was found for segregation distortion or gametic selection. This dimorphism, therefore, is a reliable marker.

• Korean Journal of Ichthyology
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• v.4 no.2
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• pp.77-81
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• 1992

Chromosome numbers of 3 species of Korea lampreys(Genus Lampetra) were investigated. Approximately 160 small and mainly acrocentric chromsomes were present in the three species of Korean lampreys. And the diploid chromosome numbers of three species were not significantly different between the species.

• The Korean Journal of Mycology
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• v.14 no.4
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• pp.253-256
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• 1986

Chromosome numbers were studied for three species of the genus Fusarium from observation of vegetative nuclear division in hyphae with aid of Giemsa-HCl techniques. It was confirmed that observation on the nuclear division could best be made at the growing hyphal tip and near the cells. The general shape of chromosome was dot-like form. The results confirmed that the chromsome number in n=8 in F. solani and F. moniliforme, and n=6 in F. cocophilum.

• Korean Journal of Microbiology
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• v.35 no.3
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• pp.192-196
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• 1999

CHEF-PFGE(Contour-Clarnped Homogeneous Electric field- Pulsed Field Gel Electrophoresis) was used to identify electrophoretic karyotype for eight strains belonging to the Fzisoriuni section Liseolo. Chromosome numbers were nine to thirteen bands, ranging in size Cram 0.75 to 6.45 Mb. The total genome size was eslimated to range from 38.19 Mb to 43.12 Mb and numerous chromosome-length polymorphisms (CLPs) were observed. For the chromosome localizalion of the gene, 1GS sequence(2.6 Kb) of rDNA from F: moniliforme, chs-2 gene(2.8 Kb) and 4 - 3 gene(3.8 Kb) from Neuuospora cmssa were wed as probes.

• The Korean Journal of Zoology
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• v.25 no.2
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• pp.81-92
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• 1982

G- and C-banding pattern analyses of striped field mice (Apodemus agrarius coreae) using 17 specimens from four localities in Korea revealed that centromeric heterochromatin results in the variation of No. 1 chromosome pair (telocentri $c_telocentric), i.e., centromeric heterochromatin sometimes appeared to be recognized as short arm. G- and C-banding patterns of four black rats (R. rattus rufescens) from two localities in Korea showed that No. 1 chromosome polymorphism (telocentri $c_telocentric) is due to pericentric inversion. In addition, G- and C-banding patterns of black rats mentioned above are idiogrammed.ammed.