• Korean Journal of Ichthyology
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• v.8 no.2
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• pp.68-73
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• 1996

This study was carried out to obtain the basic information on morphological and chromosomal charateristics in the three species of Chinese carps (grass carp; Ctenopharyngodon idellua, bighead carp; Aristichthys nobilis, and silver carp; Hypophthalmichthys molitrix) introduced to Korea from China. C. idellua was differ from A. nobilis and H. molitrix by the number of gill rakers, scales, fin rays, body proportion. A. nobilis and H. molitrix were similar in having ventral keel and many scale number, but H. molitrix was differ from A. nobilis by the connected gill rakers and body color pattern. Diploid chromosome and arm number (fundamental number, NF) of the three species were all the same to 2n=48 and NF=84. Diploid chromosome numbers in the three species are consisted of 10 pairs of metacentric chromosome, 8 pairs of submetacentric chromosome and 6 pairs of acro and/ or telocentric chromosome. Morphological and karyological relationship of the three Chinese carps are discussed.

• Environmental Mutagens and Carcinogens
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• v.16 no.2
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• pp.88-96
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• 1996

Fluorescence in situ hybridization (FISH) with the DNA probe for human chromosome 4 was used to analyse in vitro radiation induced chromosome rearrangement in peripheral lymphocyte. Translocations, dicentrics, acentrics and color junctions involving the painted chromosome were scored according to the Protocol for Aberration Identification and Nomenclature Terminology (PAINT) system. The frequency of chromosome rearrangements including reciprocal translocation, dicentric, acentric fragment and color junction increased with radiation dose. The frequency of dicentric chromosome reduced by the fixation time following irradiation, whereas that of translocation was relatively persistent. The applicability of FISH for scoring stable translocation for biological dosimetry was demonstrated.

• Genomics & Informatics
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• v.4 no.2
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• pp.80-86
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• 2006

It is clear that the construction of large insert DNA libraries is important for map-based gene cloning, the assembly of physical maps, and simple screening for specific genomic sequences. The bacterial artificial chromosome (BAC) system is likely to be an important tool for map-based cloning of genes since BAC libraries can be constructed simply and analyzed more efficiently than yeast artificial chromosome (YAC) libraries. BACs have significantly expanded the size of fragments from eukaryotic genomes that can be cloned in Escherichia coli as plasmid molecules. To facilitate the isolation of molecular-biologically important genes in Ashbya gossypii, we constructed Ashbya chromosome-specific BAC libraries using pBeloBAC11 and pBACwich vectors with an average insert size of 100 kb, which is equivalent to 19.8X genomic coverage. pBACwich was developed to streamline map-based cloning by providing a tool to integrate large DNA fragments into specific sites in chromosomes. These chromosome-specific libraries have provided a useful tool for the further characterization of the Ashbya genome including positional cloning and genome sequencing.

• Korean Journal of Plant Taxonomy
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• v.49 no.3
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• pp.269-273
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• 2019

We report the meiotic chromosome numbers of four Carex taxa from Korean populations. Three are the first reports made on taxa from Korean populations: Carex appendiculata (Trautv. & C. A. Mey.) $K{\ddot{u}}k$. ($n=27_{II}$), C. fernaldiana H. $L{\acute{e}}v$. & Vaniot ($n=33_{II}$), and C. metallica H.$L{\acute{e}}v$. ($n=15_{II}$). Reports on the other species expand the range of variation in the chromosome number within a taxon, C. miyabei Franch. (n = $43_{II}$, $44_{II}$, $45_{II}$). Carex L. (Cyperaceae) consists of more than 2,000 species worldwide and is the most species-rich genus in Korea. The species diversity in the genus has been hypothesized to be associated with the chromosome variation, but chromosome information pertaining to Korean Carex taxa is not well known. This report updates the chromosome number inventory on Korean Carex to 24 out of 180 taxa.

• Clinical and Experimental Reproductive Medicine
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• v.12 no.2
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• pp.39-57
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• 1985

Presented in this paper the data from a chromosome study of 397 patients referred for suspected chromosmal abnormalities. Karyotypes were obtained using short-term blood culture and direct method. Of these 238 patients had normal chromosome complements; 159 (40.1%) patients had chromosome abnormality. Among all patients with chromosome abnormalities, 82.4% (131/159) had aberrations of chromosome number, the others 17.60/0 (28/159\ had aberrations of chromosome structure. Ten had a chromosome rearrangement; Five of them were reciprocal and five Robertsonian translocations. Four patients with pericentric inversions and one with paracentric inversions and four with isochromosomes were observed. There were four patients with marker chromosome, two patients had a chromosome insertion; and three others. (additional abnormal chromosomes.) Thus the results of the present study indicate the importance of cytogenetic evaluation in clinically abnormal patients.

• Korean Journal of Plant Taxonomy
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• v.51 no.1
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• pp.100-102
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• 2021

The chromosome number of myoga ginger (Zingiber mioga (Thunb.) Roscoe: Zingiberaceae) has been reported as 2n = 22 for Chinese plants and 2n = 55 for Japanese plants. We checked the chromosome number of Z. mioga in plants collected in Jeollabuk-do and Jeollanam-do, Korea, and counted 2n = 44, the first report of this number for the species. As the basic chromosome number of Z. mioga is thought to be x = 11, Z. mioga plants in China, Korea, and Japan appear to be diploids, tetraploids, and pentaploids, respectively. In finding the tetraploid race of Z. mioga in Korea, we can hypothesize that the pentaploid race in Japan is derived through the fertilization of reduced gametes of the diploid race and unreduced gametes of the tetraploid race.

• Korean Journal of Plant Taxonomy
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• v.53 no.2
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• pp.166-169
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• 2023

Carex brevispicula (Cyperaceae) is endemic to Korea and is characterized by constricted achenes, short lateral spikes, and awned staminate and pistillate scales. The species classified in sect. Mitratae occurs throughout South Korea, perennating on mountains and/or rocky slopes under half shadow conditions. Meiotic chromosomes of the species were examined in this study, in which 33 meiotic cells from seven populations were found to be less than 2 ㎛ long with non-constricted chromosomes (n = 34_II). The stable chromosome number may be related to the narrow geographical distribution and/or distinct achene morphology. Further investigations of the distribution, morphological character variation, and chromosome characteristics should be conducted with closely related taxa to understand the derivation of the species and its endemism in Korea.

• The Korean Journal of Zoology
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• v.33 no.2
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• pp.222-230
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• 1990

The karyotypes of Korean Sciunis vulgaris coreas and Tamias sibiricus asiaticus were analyzed by conventional Giemsa staining and C-banding method. The diploid chromosome number (2n) of Sciunis vulgaris coreae 40 consisting of 6 metacentric, 8 submetacentric, 3 subtelocentric and 2 telocentric autosome pairs, submetacentric X and acrocentric or subtelocentric Y chromosome. The arm number (NF) of this species was obtained as 72, excluding the gonosomal arms. Tamias sibiricus asiaticus has a 2n of 38. The karyotype was represented by 3 metacentric, 4 submetacentric, 5 subtelocentric and 6 telocentric autosome paits and 2 sex chromosome. The X chromosome was submetacentric chromosome and the Y was the smallest chromosome with a median. The NF was 60. In S. vulgaris coreae constitutive heterochromatins were observed at the centromeres and telomeres. Constitutive heterochnomatins of T sibiricus asiaticus were primarily observed at the centromeres. These results suggested that non-Robensonian reanagenents and distribution of constitutive heterochromatin played an imporiant role in karyological differentiation of these species.

• Korean Journal of Animal Reproduction
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• v.15 no.2
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• pp.87-95
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• 1991

The chromosome analyses of blood culture were made of 11 Korean native and 53 crossbred males between the Korean native cattles(K) and Charolais(C), which consisted of $K\times$K, $C\times$K, $C\times$CK, CK$\times$CCK and Charolais synthetic males(CK$\times$CCK or CCK$\times$CK). 1. The diploid(2n=60, XY) Charolais synthetic male has the 29 pairs of acrocentric autosomes, a single large submetacentric X and a small metacentric Y chromosome. 2. The numbers of G-band of karyotype in these males were a few differences in the 8 pairs of autosomes(chromosome 2, 4, 5, 6, 9, 11, 19 and 26) compared to those of purebred Korean native ones. G-banding qualities were not matched in chromosome 16, 19 and 29 with the Korean native males and also in chromosome 14, 20 and 22 with other domestic cattles. 3. The G-banding pattern between chromosome 4-6-7 and 24-25-27 was alomost similar together and the possibilityof misidentification was greater in the G-banded preparations. 4. 1/29 Robertsonian translocation and other abnormalities were not observed among 11 Korean native and 53 crossbred males. This result is considered in relation to limited data and further investigation based on larger samples may be necessary for definite conclusion.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.209-214
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• 2011

Although the function and utility of RNA transcripts derived from matured spermatozoa remains unclear, they might play important roles in the establishment of a paternal genome and subsequently embryo development. Herein, we investigated the expression of X-chromosome linked RNA transcripts in matured bovine spermatozoa. The total RNA was extracted from the matured spermatozoa, and then converted to cDNA. Autosomal genes (ACT-${\beta}$ and H-2A) and X-chromosome linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) were analyzed for the characterization of X-chromosome linked RNA transcripts and compared to female fibroblasts by RT-PCR. The transcripts of autosomal genes (ACT-${\beta}$ and H2A) and X-chromosome linked genes (ANT3, HPRT, MeCP2, RPS4X and ZFX) were not detected in spermatozoa. However, XIAP (X-linked inhibitor of apoptosis protein) and XIST (X-chromosome inactive-specific transcript, a kind of paternal imprinted gene) transcripts were detected in spermatozoa, and relative levels of XIAP and XIST transcripts were similar and 0.5-fold lower when compared to female fibroblasts, respectively. Based on the findings, it is summarized that the presence of RNA transcripts of XIAP and XIST in the isolated spermatozoa may imply their role in inhibition of apoptosis and induction of X-chromosome inactivation in embryo development.