• 한국초지조사료학회지
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• 제7권3호
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• pp.162-167
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• 1987

本 실험은 등과야초인 칡에 당분을 첨가한 사일리지를 조제하여 사료배치를 향상시키기 위하여 실시하였다.칡은 줄기와 잎을 2.0~2.5cm로 절단하여 2l들이 플라스틱 용기에 물8% 첨가한 사일리지, 물8%와 전분3%첨가한 사일리지, 물8%와 포도당3% 첨가한 사일리지 그리고 아무것도 첨가하지 않은 칡 사일리지를 조제하였으며 이들과 옥수수 사일리지를 비교하였다. 사일리지의 발표품질인 유기산과 조직성분의 특성(NOF, AOF, AOL)과 pepsin-cellulase 에 의한 건물消化率(OMO)을 예정하였던 바 결과는 다음과 같이 要約된다. 1. 모든 처리의 칡 사일리지는 10% 이상의 무게 손실이었다. 2. pH에 있어서 포도당첨가 칡 사일리지가3.80, 전분첨가 칡 사일리지가 4.04, 대조구의 칡 사일리지가 4.57 그리고 물만을 첨가한 칡 사일리지는 5.34 순위로 높았다. 3. 유산함량에 있어서는 옥수수 사일리지(1.93 %), 포도당첨가 칡 사일리지 (1.89%), 전분첨가 칡 사일리지(1. 31 %), 대조구의 칡 사일리지 (0.57%), 그리고 물만을 첨가한 칡 사일리지(0.44%) 순위로 낮았다. 4. Fliegs 평점은 대조구의 칡 사일리지 22, 물첨가만의 칡 사일리지가 60, 전분 첨가한 칡 사일리지가 87이었으며, 옥수수 사일리지와 칡 사일리지는 각각 100이었다. 5. 칡 사일리지의 조단白質含量은 13.50-15.59%로서 처리간 유의성이 없었다 (P>0.05). 6. 포도당과 전분을 첨가한 힘 사열리지의 NO-F, ADF, ADL含量이 다른 處리구에 비해 유의적으로 감소되었다. (P< O. 05). 7. 포도당과 전분을 첨가한 칡 사일리지의 건물消化率은 다른 처리에 비하여 크게 增加하였다(P <0.05). 8. pH (X)와 총산(Y) 과의는 Y=-0.70X +4.96 (P< 0.01), 유산(X)과 세포내용울(Y) 과는 Y=10.61X+25.84 (P< 0.05). 그리고 리그닌 (X)과 건물소화율(Y)과는 Y=-2.48X +74.35 (P<0.05)이였다.

• Asian-Australasian Journal of Animal Sciences
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• 제2권2호
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• pp.123-128
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• 1989

An experiment was conducted to study the effect of frequency of defoliation on the ensiling characteristics and nutritive value of Guineaecotype 'A' (Panicum maximum Jacq) grass. Guinea grass harvested at 2, 3, 4, 5, 6, 7, 8, 9, 10 and 12-week cutting intervals was chopped (2 cm) and ensiled alone or with the addition of coconut (Cocos nucifera L.) meal (w/w-fresh weight basis), for periods of 6, 7 or 8 weeks in 2-litre laboratory silos. Dry matter, water soluble carbohydrates, lactic acid, pH, ash, nitrogen and digestibility in vitro were measured in representative samples of preensiled and ensiled material. Dry matter content of silages prepared from herbage harvested at 2 and 3 weeks intervals was lower (P<0.05) compared to longer defoliation intervals. The pH of silage prepared from herbage over 8 weeks old were higher (P<0.05) than those harvested below 8 weeks. The water soluble carbohydrate content of silage prepared from 3-weekly defoliated herbage was higher (P<0.05) than those prepared from longer periods. Lengthened growth period decreased (P<0.001) the crude protein content of the ensiled material, whereas the addition of coconut meal resulted in marginal increases. In vitro organic matter digestibility of the silages decreased (P<0.05) with the increase in forage maturity.

• Asian-Australasian Journal of Animal Sciences
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• 제28권9호
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• pp.1281-1287
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• 2015

This study was performed to determine the effect of field pea silages which were the organic acid (OA) alternative of oregano and cinnamon essential oils on fermentation quality and aerobic stability. Whole crop pea was harvested at full pod stage and wilted in the laboratory at the 48 h. The chopped pea was mixed and divided into equal portions allocated to five groups: CON (non-treated), distilled water, denoted as control group; OA group, a mixture of 60% formic acid, 20% sodium formate and 20% water applied at a rate of 5 g/kg fresh forage (Silofarm Liquid, Farmavet); origanum (ORE) group, Origanum onites essential oil at 400 mg/kg fresh forage; cinnamon (CIN) group, cinnamon essential oil at 400 mg/kg fresh forage; origanum+cinnamon (ORECIN) group, a mixture of ORE and CIN applied at an equal rate of 400 mg/kg fresh forage. Cinnamon decreased acetic acid (AA), ammonia nitrogen ($NH_3-N$) and weight loss (WL) at the end of 60 days silage. Crude protein (CP) and dry matter (DM) increased by cinnamon essential oil. Yeasts were not detected in any treatments, including the control, after 7 days of air exposure. The $CO_2$ amount decreased and the formation mold was inhibited in the aerobic period by the addition of cinnamon oil. Oregano did not show a similar effect, but when it was used with cinnamon, it showed synergic effect on AA and during aerobic period, it showed antagonistic effect on mold formation and DM losses. It was found in this study that cinnamon can be an alternative to organic acids.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.812-815
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• 2005

This study was conducted to see the influence of additives and fermentation periods on Mott grass silage (MGS) characteristics, its chemical composition and to compare the digestion kinetics of Mott grass (MG) and MGS in Nili buffaloes. Mott grass chopped with a locally manufactured chopper was ensiled using two additives, cane molasses and crushed corn grains each at 2, 4 and 6% of forage DM for 30 and 40 days in laboratory silos. The pH, lactic acid concentration, dry matter (DM), crude protein and fiber fractions of MGS were not affected by the type or level of additive and fermentation periods. The non-significant pH lactic acid concentration, and chemical composition of MGS indicated that the both molasses and crushed corn were utilized at similar rate for the growth of lactic acid bacteria and production of organic acids. The MG ensiled with molasses at 2% of fodder DM for 30 days was screened out for in situ digestion kinetics in Nili buffaloes. Ruminal DM and neutral detergent fiber (NDF) degradabilities of MGS were significantly (p<0.05) higher than that of MG. The DM and NDF rate of degradation, lag time and extent of degradation was nonsignificant between MGS and MG. The higher ruminal degradation of DM and NDF of MGS than MG was probably a reflection of fermentation of MG during ensilation that improved its degradability by improving the availability of easily degradable structural polysaccharides to ruminal microbial population. The results in the present study have indicated that MG ensiled with either 2% molasses or 2% crushed corn for 30 days has better nutritive value for buffalo.

• Asian-Australasian Journal of Animal Sciences
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• 제15권2호
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• pp.218-221
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• 2002

Whole forage sorghum (saccharatum) cultivar FS5 was harvested at the soft dough ($303{\pm}9g\;kg^{-1}$ DM) stage of maturity. The sorghum was chopped into approximately 20 mm pieces and ensiled under laboratory conditions in 1.5 L Weck glass jars. At ensiling, it was treated with two commercial silage inoculants: Pioneer 1188 (Inoculant A) and Eco-corn (Inoculant B). The inoculant A and B was applied at ca $2{\times}10^5$ or $2{\times}10^4$ colony forming units $g^{-1}$ DM., respectively. Silage with no additives served as a control. Three jars per treatment were opened on days 2, 4, 8, 15 and 60 post-ensiling to study fermentation dynamics. After 60 days of ensiling the silages were analyzed and subjected to an aerobic stability test lasting 5 days. Results showed that both inoculants caused a more rapid rate of pH decrease and a higher amount of lactic acid production. All the silages were well preserved and were stable upon exposure to air. Inoculants did not influence (p>0.05) the ash and total N contents, but tended to reduce acetic acid (p<0.05), butyric acid (p<0.01) and propionic acid (p<0.01) contents, and to increase the lactic acid content (p<0.01). The lower DM content of silages treated with Inoculant A agrees with the greater gas loss resulting from the DM loss, which was in good agreement with the higher yeast counts upon aerobic exposure. Silage treated with inoculant B had the highest DM (p<0.05) and lactic acid contents (p<0.01), and the lowest acetic acid content (p<0.05), which agrees with the rapid reduction of pH and smaller gas loss. Inoculant B reduced the ADF (p<0.01), ADL and NDF (p<0.05) contents, which also indicates smaller losses of organic soluble material. The control silages contained the highest levels of volatile fatty acids but no lactic acid, indicating secondary fermentation. It was concluded that both inoculants may improve the fermentation process, since silages from all treatments were stable upon aerobic exposure, noadvantage could be attributed to any of the inoculants used.

• Animal Bioscience
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• 제34권6호
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• pp.1029-1037
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• 2021

Objective: This study was conducted to investigate effects of antifungal substance and carboxylesterase-producing inoculant on fermentation indices and rumen degradation kinetics of whole crop rice (WCR) silage ensiled at different dry matter (DM) contents. Methods: Dual-purpose inoculants, Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1, confirmed both activities of antifungal and carboxylesterase in the previous study. The WCR at mature stage was chopped, and then wilted to obtain three different DM contents consisting of 35.4%, 43.6%, and 51.5%. All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1×105 colony forming unit/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d. Results: The INO silages had lower lactate (p<0.001) and butyrate (p = 0.022) with higher acetate (p<0.001) and propionate (p<0.001) than those of CON silages. Ammonia-N (p<0.001), lactate (tendency; p = 0.068), acetate (p = 0.030), and butyrate (p<0.001) concentrations of INO silages decreased linearly with increasing DM content of WCR forage. The INO silages presented higher lactic acid bacteria (p<0.001) with lower molds (p<0.001) than those of CON silages. Yeasts (p = 0.042) and molds (p = 0.046) of WCR silages decreased linearly with increasing DM content of WCR forage. In the rumen, INO silages had higher the total degradable fraction (p<0.001), total volatile fatty acid (tendency; p = 0.097), and acetate (p = 0.007), but lower the fractional degradation rate (p = 0.011) and propionate (p<0.001) than those of CON silage. The total degradable fraction (p<0.001), total volatile fatty acid (p = 0.001), iso-butyrate (p = 0.036), and valerate (p = 0.008) decreased linearly with increasing DM content of WCR forage, while the lag phase (p<0.001) was increased linearly. Conclusion: This study concluded that application of dual-purpose inoculants on WCR silage confirmed antifungal and carboxylesterase activities by inhibiting mold and improving rumen digestibility, while increase of wilting times decreased organic acids production and rumen digestibility.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.920-924
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• 2007

Two experiments were carried out on a laboratory scale. The first involved a study of the effect of green tea on characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB). FJLB was treated with 50 g/L of green tea products as follows: new shoot powder (FJLB+N), leaf powder (FJLB+L), commercial powder (FJLB+P), sterilized new shoot powder (FJLB+SN), sterilized leaf powder (FJLB+SL) or sterilized commercial powder (FJLB+SP). FJLB without any additive was also prepared (Untreated FJLB). After incubation, the number of microorganisms in FJLB were studied. Subsequently, these FJLB were applied at 10 ml/kg to chopped rhodesgrass to study their effects on fermentation. Compared with untreated FJLB, the addition of green tea increased (p<0.05) lactic acid bacteria (LAB) and also aerobic bacteria counts in FJLB. At 60 d of ensiling, all the FJLB treated silages were well preserved, pH and butyric acid content were lower (p<0.001) and lactic acid was higher (p<0.001) than that of the control. Lactic acid content was significantly higher (p<0.001) with treated FJLB than with untreated FJLB. FJLB treated with sterilized green tea decreased (p<0.001) the pH and the lactic acid content was higher (p<0.001) than that in the unsterilized green tea silages.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1541-1552
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• 2004

The effect of eight varieties of whole crop rice silage (WCRS) harvested at four stages of maturity were investigated for in situ DM and N degradability, ME and MP yield and content in an 8${\times}$4 factorial experiment. The varieties were Akichikara, Fukuhibiki, Habataki, Hamasari, Hokuriku 168, Kusanami, Tamakei 96 and Yumetoiro. Hamasari and Kusanami were forage varieties while all others were grain varieties. Forages were harvested on 10, 22, 34 and 45 days after flowering, ensiled and kept in airtight condition. Between 45 and 49 days after ensiling, silages opened, chopped and milled green to pass through 4 mm screen. Samples were incubated in the rumen of two Holstein steers for 0, 3, 6, 9, 12, 24, 48, 72 and 96 h over eight 4 d periods. Bags at 0 h were washed in a washing machine. Variety affected DM (p<0.001: except 'a+b', p<0.01) and N (p<0.001) degradability characteristics of WCRS. Stages of maturity also affected DM (p<0.001: except 'a+b', p<0.05; 'c', p<0.08) and N (p<0.01: except 'c', p<0.05) degradability characteristics of WCRS. Interactions between variety and stages of maturity occurred in all DM (p<0.001) and N (p<0.001) degradability characteristics except (p>0.05) for DM 'b', DM 'c', DM 'a+b' nd N 'c'. Effective DM degradability was higher in grain varieties than forage varieties and degradability increased with maturity. N availability decreased only slightly with maturity. Variety was the key factor for N degradability characteristics of WCRS since variety accounted for most of the total variation for degradability characteristics. Both ME and MP content and yield were higher (p<0.001) in grain varieties, and they increased (p<0.001) with the maturity. The results clearly demonstrated that the grain type varieties contained higher ME and MP content than forage varieties, and increase in maturity increases both ME and MP content of WCRS.