• The Journal of the Korea Contents Association
• /
• v.19 no.9
• /
• pp.521-534
• /
• 2019

From the day of luxurious inland river cruise ship started in Chongqing, China, the controversy is continuous; some people think those luxurious cruise ships on inland river might reduce the experience to enjoy the scenery of the Three Gorges, while some people think, which could improve the experience. In the study, the service facilities provided by luxurious inland river cruise ship will be evaluated by Pre-Post comparison based on IPA methods. After a review of the service facilities provided by luxurious inland river cruise ship running on Three Gorges areas. The tourists who would take the trip were asked to fill an questionnaire to examine the desired service facilities performance, which represents the highest level of the service facilities of the cruise ship that is expected by customers. After the trip, they were asked again to fill another questionnaire to examine the importance of service facilities and the performance of service. Pre-Post comparison would be examined to tell whether there is any change between pre and post experience. IPA would be used to evaluate the service facilities provided by inland river cruise ship. At last, some strategic suggestions to the development of inland river cruise ship are made.

• The Journal of the Convergence on Culture Technology
• /
• v.5 no.1
• /
• pp.227-234
• /
• 2019

Hyochang Park was the royal tomb of the Joseon Dynasty, where were the tombs of Prince Munhyo and his birth mother Ui-bin Seong, king Soonjo's concubine Sook-Ui Park and Youngon princess by a concubine. It was upgraded from Hyochang tomb to Hyochangwon at King Gojong. The Japanese imperialism destroyed Hyochangwon and made it into a park, also moved to Seosamneung in 1944. After returning from Chongqing after liberation, Kimgu made the independence movement tomb in the empty space of Hyochang Park. There were 3 martyr's tomb, provisional government figure tomb. Kimgu himself was buried in Hyochang Park after his death in 1949. During the regime of Rhee Seung-man and Park Jeong-hee, there was a constant attempt to change the identity of Hyochang Park, where independence activists were buried. Hyochang Stadium, Wonhyo buddhist priest's Statue and North Korean Anti-Communist Tower were established. After democratization in 1987, the independence activist tomb of Hyochang Park began to gain meaning again. The 6th Republic succeeded the provisional government in the Constitution, also the national cemetery and independent park of Hyochang Park were attempted but the social consensus could not be achieved. This study focuses on the historical middle layer and symbolism of Hyochang Park and aims to contribute to social consensus on the identity of Hyochang Park by making a storytelling on the theme of 'Independence Road'.

• Animal Bioscience
• /
• v.35 no.2
• /
• pp.184-195
• /
• 2022

Objective: In this study we aimed to evaluate the effect of dietary live yeast supplementation on ruminal pH pattern, fermentation characteristics and associated bacteria in beef cattle. Methods: This work comprised of in vitro and in vivo experiments. In vitro fermentation was conducted by incubating 0%, 0.05%, 0.075%, 0.1%, 0.125%, and 0.15% active dried yeast (Saccharomyces cerevisiae, ADY) with total mixed ration substrate to determine its dose effect. According to in vitro results, 0.1% ADY inclusion level was assigned in in vivo study for continuously monitoring ruminal fermentation characteristics and microbes. Six ruminally cannulated steers were randomly assigned to 2 treatments (Control and ADY supplementation) as two-period crossover design (30-day). Blood samples were harvested before-feeding and rumen fluid was sampled at 0, 3, 6, 9, and 12 h post-feeding on 30 d. Results: After 24 h in vitro fermentation, pH and gas production were increased at 0.1% ADY where ammonia nitrogen and microbial crude protein also displayed lowest and peak values, respectively. Acetate, butyrate and total volatile fatty acids concentrations heightened with increasing ADY doses and plateaued at high levels, while acetate to propionate ratio was decreased accordingly. In in vivo study, ruminal pH was increased with ADY supplementation that also elevated acetate and propionate. Conversely, ADY reduced lactate level by dampening Streptococcus bovis and inducing greater Selenomonas ruminantium and Megasphaera elsdenii populations involved in lactate utilization. The serum urea nitrogen decreased, whereas glucose, albumin and total protein concentrations were increased with ADY supplementation. Conclusion: The results demonstrated dietary ADY improved ruminal fermentation dose-dependently. The ruminal lactate reduction through modification of lactate metabolic bacteria could be an important reason for rumen pH stabilization induced by ADY. ADY supplementation offered a complementary probiotics strategy in improving gluconeogenesis and nitrogen metabolism of beef cattle, potentially resulted from optimized rumen pH and fermentation.

• Animal Bioscience
• /
• v.36 no.1
• /
• pp.29-42
• /
• 2023

Objective: Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes. Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs. Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (CDK8) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (YBX1) was a crucial transcription factor for CDK8, which mediated the effect of CDK8 in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs. Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. CDK8 was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.