• Journal of the Korean Chemical Society
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• v.46 no.2
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• pp.157-163
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• 2002

To identify RNA motifs interacting with $tRNA^{Val}$, a SELEX(Systematic Evolution of Ligands by Exponential Enrichment) was applied. Random DNA library which contains a region of ran-domized 48-mer oligonucleotide flanked by conserved sequ ence primers was transcribed into RNA pool using T7 RNA polymerase and RNA aptamers were selected with $tRNA^{Val}$ -immobilized affinity column through 14 rounds of SELEX. Some of the resulting aptamers contained a consensus sequence similar to the sequence in the loop regions of three rRNAs; C43GAAC47 sequence of 5S rRNA, G1491AAGU1495, G1379UUCC1383 sequence of 16S rRNA and C1064UUAG1068, G2110UGUA2114, C2480GACGG2485, A2600CAGU2604 sequence of 23S rRNA. These results suggest that $tRNA^{Val}$ can interact with 5S rRNA, 16S rRNA and 23S rRNA with variety in ribosome.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.05a
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• pp.477-480
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• 2002

Transparency of position that provide quick service regardless of physical position to users in distribution computing environment is getting into prominence and is developed in component form that many application softwares take advantage of distributed object technology. Because design object in distribution computing environment and OMG introduces OMA for embody, defined CORBA by standard of distributed object technology on OMA's abstract picture object model. Security service in CORBA platform is very important. Present object security service in distribution computing environment that refer standard of sorority and security models of distribution computing platform in CORBA in this treatise and follow in CORBA security service rules.

• Journal of Navigation and Port Research
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• v.30 no.4
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• pp.309-314
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• 2006

Community structure and biodiversity of benthic marine algae were studied in the rocky shore of Phohang New Port, Yeoungil Bay. A total of 79 species of marine algae including 8 Chlorophyta, 23 Phaeophyta and 48 Rhodophyta are listed. The dominant algal species were Corallina pilulifera in all seasons, and Sargassum homeri in winter. Lomentaria catenata, Lomentaria hakodatensis, Grateloupia laceolata, Sargassum thunbergii, Chondria crassicaulis, Ulva pertusa, and Hypnea charoides were subdominant at different seasons. The algal biomass per unit area of the benthic algal community in Pohang New Port under construction was 14506 - 398.93 g dry wt m-2, which means that its annually averaged value is 273.45 g dry wt m-2 . The seasonal change in algal biomass showed highs in winter and spring, and lows in summer and autumn. The algal species diversity (H') based on algal biomass was 2.07 annually in average, with 2.42 in winter, 2.65 in spring, 1.79 in summer and 1.43 in autumn. It was high in winter and spring seasons and low in summer and autumn seasons. It is caused by that spring and winter are the profitable growth time for algae and species components in this study were dominated by the annual or seasonal rather than perennial plants.

• Archives of Pharmacal Research
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• v.27 no.2
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• pp.217-224
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• 2004

Previously, we reported that water-extracted Acanthopanax senticasus exhibited anti-meta-static activity by stimulating the immune system. In this study, we fractionated glycoproteins (EN-SP) from the soluble protein layer (GF-AS) of A. senticasus and determined their basic chemical properties. We also investigated the anti-tumor and immunostimulating activities of the fractionated glycoprotein, EN-SP. We found that intravenous (i.v.) administration of GF-AS dramatically inhibited metastasis of colon26-M3.1 carcinoma cells to the lung in a dose-dependent manner. In vitro analysis showed GF-AS to enhance the proliferation of splenocytes. GF-AS also stimulated peritoneal macrophage, which was followed by the production of various cytokines such as IL-1$\beta$, TNF-$\alpha$, IL-12 and IFN-${\gamma}$. Furthermore, the production of these cytokines was partially blocked when peritoneal macrophage was cultured with the polyclonal antibodies against GF-AS. The depletion of NK cells by rabbit anti-asialo GM1 serum partly abolished the inhibitory effect of GF-AS on lung metastasis of colon26-M3.1 cells. Using gel filtration, EN-SP, an active glycoprotein fraction, is isolated from GF-AS. While both GF-AS and EN-SP stimulated the proliferatation of splenocytes of normal mice, EN-SP showed higher anti-metastatic activity and more potently stimulated the proliferation of splenocytes compared to GF-AS. These results suggest the use of EN-SP, the fractionated glycoprotein from A. senticasus, can be used as a therapeutical reagent to prevent or inhibit tumor metastasis.

• Research in Community and Public Health Nursing
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• v.12 no.1
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• pp.115-124
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• 2001

This study was designed to investigate the effects of a smoking cessation school program on attitudes towards smoking cessation, the subjective norm, perceived behavioral controls, the intent to cease smoking and smoking behavior. Also included was the goals of the program itself. Study subjects were 80 adolescent smokers who at least attended the smoking cessation school program without absence. had smoked one cigarette at the time of the first study before the program and attended the first. second. and third studies (The study was conducted from September 26. to December 21. 1999. a period of 59 days). Data collection was performed using the TPB questionnaire which was developed by Jee Yon-Ock(1994). The survey included questions covering the intended areas of study mentioned above. The first data was collected during the first day of the five day program. The second survey was conducted immediately after the last day of the program and the last survey was four weeks later. Data was analyzed with a SAS/PC program including N. %. a paired t-test. The results were as follows; 1. After the program. the scores representing attitude towards smoking cessation (before program: 14.5. after program: 16.8) and perceived behavioral control (before program: 1.2. after program: 2.1) rose significantly, but the scores for attitudes towards the subjective norm and the intent to cease smoking were not notably different. 2. After the program. the frequency of daily smoking fell significantly (before program: 5.7 cigarettes. after program: 3.2 cigarettes). In conclusion, the five day smoking cessation school program increased attitude. perceived behavioral control and decreased smoking behavior but did not influence subjective norm, smoking cessation intention. Thus, further study is required to better evaluate the effects of the program and to improve any shortcomings.

• Journal of Environmental Impact Assessment
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• v.11 no.1
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• pp.1-12
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• 2002

중부지방 3백만 주민의 상수원으로 이용되는 대청호는 부영양화가 심화되어 적절한 수질관리가 없으면 상수원수의 최저등급인 3급수를 만족할 수 없는 상태에 이르고 있다. 그래서 2002년 1월에 금강수계 물관리 및 주민지원 등에 관한 법률이 제정되었는 바, 오염총량제와 수변구역의 지정을 통한 수질보전 등을 주요한 내용으로 포함하고 있다. 오염총량제를 실천하기 위해서는 대청호의 환경용량을 추정하는 것이 필수 불가결하다. 환경용량의 추정은 장래 대청호 수질영향권역에서 개발사업이 이뤄질 때 환경영향평가 특히 누적영향평가를 위해 필요하다. 대청호의 환경용량을 추정하기 위해 오염부하량과 유량의 변화가 대청호의 총질소와 총인농도에 미치는 영향을 WASF5모형으로 분석하였다. 총질소 부하량이 50%증가한다고 가정했을때 갈수기. 명수기, 홍수기에 각각 0.14-0.29 mg/l, 0.12-0.16 mg/l and 0.03-0.05 mg/l 정도의 농도 변화가 예측되었다. 총질소 부하량이 지금의 반이하로 감소하더라도 총질소농도 5급수기준 (1.0-1.5 mg/l)을 달성할 수가 없다. 대청호의 환경용량을 상수원수 3급수기준으로 산정한다면, 그것은 현재의 총질소 부하량의 반보다 훨씬 적다고 추정할 수 있으며 따라서 총질소 부하량은 지금의 반이하로 줄어들어야 할 것이다. 총인 부하량이 50%증가한다고 가정했을 때 갈수기, 평수기, 홍수기에 각각 0.005-0.011 mg/l, 0.004-0.006 mg/l and 0.001-0.002 mg/l 정도의 농도 변화가 예측되었다. 총인 부하량이 지금의 반이하로 감소하더라도 총인 농도 4급수기준 (0.05-0.1 mg/l)을 간신히 달성할 수 있다. 따라서 대청호의 환경용량은 지금의 총인 부하량의 반정도로 추정할 수 있으며 총인 부하량은 지금의 반이하로 줄어들어야 할 것이다. 오염부하량을 줄이기 위한 방안을 정오염원과 비정오염원으로 나눠 제시하였고 오염총량제 실현방안을 제시하였다.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.5
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• pp.10-18
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• 2005

The research was carried out to define the morphological and growth characteristics of Poa pratensis L.(Kentucky bluegrass) 'Sewon' in native to Korea. Poa pratensis 'Sewon' was collected at the terrace land on the river in Guiman-ri, Cheongcheon-myeon, Goesan-gun, Chungcheongbuk-do on May 5, 1995. The collected Poa pratensis 'Sewon' was grown under non-mowing naturally growing condition. The morphological characteristics of Poa pratensis 'Sewon' are as follows. : The height of plant was 21.6cm but was increased to 33cm at the flowering time. The height of first node from the base was 5.0cm and the length and width of leaf were 9.6cm and 4.1cm, respectively. The length of inflorecences was 37.8cm and the length of a spike was 7.3cm. Based on these morphological characteristics, Poa pratensis 'Sewon' was defined to dwarf~medium and coarse texture type Kennturky bluegrass. 1 to 9 scale of visual quality, visual color and visual density were measured. Poa pratensis 'Sewon' showed best turf qualities among native Kentucky bluegrasses. In spring and fall growing season, Poa pratensis 'Sewon' were measured to be excellent performances for turf qulities similar to foreign excellent quality cultivars such as Midnight, Nuglade and Brilliant Kentucky bluegrasses. But turf quality of Poa pratensis 'Sewon' decreased by plant disease according to temperature increase in summe compared to foreign excellent quality cultivars. So, Poa pratensis 'Sewon' should be improved of disease tolerent quality by incessant breeding work in order to compete foreign excellent quality cultivars.

• Analytical Science and Technology
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• v.13 no.1
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• pp.72-80
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• 2000

The HS/GC/MS method was performed to analyze the volatile organic compounds in waste sludge samples. This study was performed to establish the fundamental data by studying the effects of salt, equilibrium temperature and time in the volatile organic compounds analysis. The presence of salts have been found to increase the sensitivity. The peak area is increased from 1.07 to 2.61 times by adding the salts to the water sample, compared with a salt tree sample. The recoveries of target compounds have found between 90% and 127% at sample temperature of $85^{\circ}C$ for 30 min. This HS/GC/MS method can be applied to analyze the volatile organic compounds and organohalo compounds in the environmental matrix.

• Analytical Science and Technology
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• v.10 no.1
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• pp.75-81
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• 1997

Poly-${\beta}$-hydroxybutyrates(PHB) are well known intracellular biopolymer which are completely degraded to carbon dioxide and water in the landfill. The pressurized fermentation method was used to increase the cell growth of Alcaligenes eutrophus and productivity of PHB. The experimental data were analyzed in terms of carbon source and gas composition effects at various elevated pressures and temperatures. The results reveal that the flow rate of hydrogen gas of 0.0075vvm for the culture is better PHB production than the no hydrogen flow when fructose was used as a sole carbon source. The higher yields and productivities of PHB biosynthesized by A. eutrophus were obtained when the oxygen composition was changed from 2% to 8% at 6atm and $30^{\circ}C$.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2006.05a
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• pp.829-832
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• 2006

The word P2P implies significant changes in current business dynamics. The P2P service enables individuals to be connected to the Internet for the direct provision of information and even downloads from one another without the conventional method of passing through search engines. This can be utilized to extend the path of retrieving information from limited web sites to personal and enterprise databases. That is, it is now possible for individuals to manage their own information on a national or global scope, share various information with other members, form communities of users interested in sharing homogeneous information, and utilize remote conference and remote education using groupware.