• Journal of the Korean Professional Engineers Association
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• v.18 no.4
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• pp.12-22
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• 1985

This investigation was attempted to determine changes in chlorophyll. Pheophytin content PH and titratable acidity during young redish Kim chi fermentation. Young redishes divied in to leaf and vein and fermented in dark room for 35 days at 10$^{\circ}C$ after pickling each group. Samples were collected at 5 days interval for the chemical analysis. The following results were obtained. 1. Chlorophyll content in young redish was decreased by decreasing PH during fermentation periods. Chlorophyll content decreased from 11.60mg/g to 7.62mg/g in leaf, from 0.48mg/g to 0.33mg/g in vein respectively during 35 days fermentation periods. In contrast to chlorophyll. pheophytin content increased as chlorophyll content decreased during the period. The ratio of chlorophyll a and b content in young redish about 3 : 1 in leaf and vein and decreased nearly the same ratio during fermentation periods. In case of pheophytin a and b it was increased without any relation with the ratio chlorophyll a and b. In generally, the optimum PH for eating was known 4.3 to 5.0. Young redish Kim chi was reached the optimun PH at 15 to 25 days of fermentation at 10$^{\circ}C$. At this periods. Chlorophyll content varied from 11.32mg/g to 9.30mg/g for leaf and from 0.43mg/g to 3.37mg/g for vein, respectively. 2. Titratable acidity(as lactic acid) was increased to 0.54% in leaf at 20 days fermentation period during Kim chi fermentation. and nearly unchanged until 30days. At the end of fermentation period the titratable acidity was about 0.30％. Titratable acidity was increased as decreasing pH. and the correlation coefficients was -0.92 in leaf and -0.77 in Vien. 3. Correlation between titratable acidity and chlorophll, pheophytin content was not significant statistically.

• Journal of Photoscience
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• v.9 no.2
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• pp.74-77
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• 2002

Chlorophyll (Chi) d was assigned to an antenna pigment of red algae in 1943, but its presence and function in red algae have not been necessarily clear for a long time. In 1996, it was shown that Chi d functioned as a major antenna pigment in a peculiar oxygenic photosynthetic prokaryote, Acaryochloris marina, isolated as a symbiont of a colonial ascidian from coral reefs. This finding evoked the necessity for reexamination of the presence and function of Chi d in red algae. We found Chi d in methanol-extract from several marine red algae, and the relative content was high in one species, Ahnfeltiopsis flabelliformis. Absorption and fluorescence spectra, HPLC analysis, and NMR and mass spectroscopy characterized Chi d extracted from the red algal thalli, and those were essentially identical to those of Chi d isolated from A. marina. However, micro-spectrophotometric analysis suggested that Chi d was not an actual constituent of the red algae but came from epiphyte(s) attached to surface of red algal thalli.

• Journal of Photoscience
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• v.9 no.2
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• pp.70-73
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• 2002

A cyanobacteria-like organism Aaryochloris marina performs oxygenic photosynthesis with near far-red light by the use of chlorophyll d. Reaction center chlorophyll (Chl) of Photosystem (PS) II of A. marina was studied by analysis of millisecond-delayed fluorescence. Delayed fluorescence is emitted by Chi d indicating efficient energy transfer between antenna Chi d molecules and the unknown primary electron donor of PS II. P740 a reaction center Chl of PS I of A. marina is shown to give a dimer type cation, and triplet state with a D value of 245xlO$\^$-4/ cm$\^$-l/ in contrast to the 280-290 xlO$\^$-4/cm$\^$-l/ values of P700 suggesting triplet spins interacting at a 5% larger distance in P740 than in P700.

• Journal of Photoscience
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• v.9 no.2
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• pp.367-369
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• 2002

Chlorophyll (ChI) a' is the Cl3$^2$-epimer of ChI a which is the constituent of P700, the primary electron donor of Photosystem (PS) I, of a thrmophilic cyanobacterium, Synechococcus elongatus, whose structure was recently determined by X-ray crystallography. To determine whether PS I of diverse oxygenic photosynthetic organisms universally contain one molecule of ChI a ’, pigment compositions of thylakoid membranes and PS I complexes isolated from cyanobacteria, green algae, red algae and higher plants were determined by reversed-phase HPLC. The results show that involvement of one ChI a'molecule in PS I is the universal feature for Chi a-based PS I of oxygenic photosynthetic organisms.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.4
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• pp.231-237
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• 1973

Eeffect of temperature (3-day or 7-day treatment under $15^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$) on chlorophyll, nitrogen and potassium content in the second leaf blade from top of IR667 line (Suwon 213 and 214) and leading local varieties (Jinheung and Paldal) was investigated at the end of nursery, maximum tillering and flowering stage using phytotron. 1. Chlorophyll content was higher in IR667 line than in the local at $25^{\circ}C$ but reversed at $20^{\circ}C$ or $15^{\circ}C$ and chlorophyll a/b value was always high in the local. 2. Chlorophyll content per fresh weight decreased with growth and low temperature effect was greater at the end of nursery and on chlorophyll a than on chlorophyll b. 3. Chlorophyll a/b value increases with the increase of chlorophyll content and the increasing rate of chl. a/b value per chlorophyll increment tends to decrease under unfavorable condition. This decrease is greater in low temperature sensitive IR667 than local varieties. 4. According to chlorophyll retention value IR667 line was weaker at low temperature. 5. The content of total nitrogen or soluble nitrogen (methanol soluble) was decreased by low temperature. 6. Chl. (a+b)/S-N value decreased with growth and seemed not to be greatly affected by temperature and always higher in the local. 7. Potassium content (total or methanol soluble) tends to decrease at low temperature and soluble K increased with chlorophyll content. 8. High yielding ability of IR667 seems to be attributable to its high chlorophyll content at high temperature and easy leaf discoloration by low temperature or by nitrogen depression of IR667 seems to be attributable to the low Chl./S-N value.

• Ocean Science Journal
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• v.41 no.4
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• pp.301-313
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• 2006

Abundance and distribution of phytoplankton in seawater at southwestern East/Japan Sea near Gampo were investigated by HPLC analysis of photosynthetic pigments during summer of 1999. Detected photosynthetic pigments were chlorophyll a, b, $c_{1+2}$ (Chl a, Chl b, Chl $c_{1+2}$), fucoxanthin (Fuco), prasinoxanthin (Pras), zeaxanthin (Zea), 19'-butanoyloxyfucoxanthin (But-fuco) and beta-carotene (B-Car). Major carotenoid was fucoxanthin (bacillariophyte) and minor carotenoids were Pras (prasinophyte), Zea (cyanophyte) and But-fuco (chrysophyte). Chl a concentrations were in the range of $0.16-8.3\;{\mu}g/land$ subsurface chlorophyll maxima were observed at 0-10m at inshore and 30-50 m at offshore. Thermocline and nutricline tilted to the offshore direction showed a mild upwelling condition. Results from size-fraction showed that contribution from nano+picoplankton at Chl a maximum layer was increased from 18% at inshore to 69% at offshore on average. The maximum contribution from nano+picoplankton was found as 87% at St. E4. It was noteworthy that contribution from nano+picoplanktonic crysophytes and green algae to total biomass of phytoplankton was significant at offshore. Satellite images of sea surface temperature indicated that an extensive area of the East/Japan Sea showed lower temperature ($<18\;^{\circ}C$) but the enhanced Chi a patch was confined to a narrow coastal region in summer, 1999. Exceptionally high flux of low saline water from the Korea/Tsushima Strait seemed to make upwelling weak in summer of 1999 in the study area. Results of comparisons among Chi a from SeaWiFS, HPLC and fluorometric analysis showed that presence of Chi b cause underestimation of Chi a about 30% by fluorometric analysis but overestimation by satellite data about 30-75% compared to HPLC data.

• Journal of Microbiology
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• v.45 no.2
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• pp.98-104
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• 2007

Chlorophyll ${\alpha}$ concentration and cyanobacterial cell density are regularly employed as dual criteria for determinations of the alert level for cyanobacterial bloom. However, chlorophyll ${\alpha}$ is not confined only to the cyanobacteria, but is found universally in eukaryotic algae. Furthermore, the determination of cyanobacterial cell counts is notoriously difficult, and is unduly dependent on individual variation and trained skill. A cyanobacteria-specific parameter other than the cell count or chlorophyll ${\alpha}$ concentration is, accordingly, required in order to improve the present cyanobacterial bloom alert system. Phycocyanin has been shown to exhibit a strong correlation with a variety of bloom-related factors. This may allow for the current alert system criteria to be replaced by a three-stage alert system based on phycocyanin concentrations of 0.1, 30, and $700\;{\mu}g/L$. This would also be advantageous in that it would become far more simple to conduct measurements without the need for expensive equipment, thereby enabling the monitoring of entire lakes more precisely and frequently. Thus, an alert system with superior predictive ability based on highthroughput phycocyanin measurements appears feasible.

• Journal of Photoscience
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• v.9 no.2
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• pp.385-387
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• 2002

Direct EPR evidence of the photo-generation of superoxide radicals ( $O_2$$^{-.}$) was obtained by using spin trapping techniques in spinach photosystem II (PSII) membranes. $O_2$$^{-.}$ was detected by following the formation of 5-diethoxyphosphoryl-5-methyl-1 -pyrroline-N-oxide (DEPMPO) superoxide adducts, DEPMPO-OOH. The significant increase of the EPR signal amplitude of DEPMPO-OOH in N$H_2O$H-, CaC $l_2$- and NaCl-treated PSII membranes showed that the oxygen-evolving system has a close relation to the $O_2$$^{-.}$ production. PSII membranes with inactivated donor side could not prevent the $O_2$$^{-.}$ production efficiently. Treatments on PSII donor side also influence the maximum level and the kinetics of Chlorophyll (Chi) a fluorescence. Results suggested that manganese cluster and extrinsic proteins might affect Chi a fluorescence in ways different from that happens at the acceptor side of PSII.SII.SII.

• Proceedings of the KSRS Conference
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• 1998.09a
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• pp.95-100
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• 1998

The purpose of this study is to describe the change of the spring bloom and oceanographic condition. The variation of pigment concentration derived from the satellite ocean color data has been analyzed. According to the movement of blooming area, blooming was very concerned with a rising trend of sea surface temperature and a supply of nutrients. A nutrient rich water carried by the Oyashio encounters with the warm Core ring, where mixings and blooms are observed. We examined the correlation by using the satellite observations of the temperature and chlorophyll-a for the spring seasons (May, June, July) of 1998 the off Sanriku area (38-43N, 141- l50E). Using the SeaWiFS data, we process the data into the level-3, which contains the geophysical value of chlorophyll-a. And chlorophyll-a data is mapped for the water between 110E and 160E, and 15N and 52N with a 0.08 * 0.05 degree grid for each image. And Sea Surface Temperature (SST) data is produced using the AVHRR onboard the NOAA. The SST is derived by the MCSST. Then, the data is mapped for the water as much as chi-a data. And these gridded image was made by detection of each water masses, which are Kuroshio Extension, the warm-core ring and the Oyashlo Intrusion, etc., using those satellite images to determine short term change. Off Sanriku is a place where warm-water pool and the Oyashio at-e mixed. When warm streamer has intruded in cold water, the volume of phytoplankton increases at the tip of warm streamer. Warm water streamer was trigger of occurring blooming. And also, SeaWiFS images provided as much information for the studies of chlorophyll-a concentrations in the surface.

• Journal of Korean Society of Forest Science
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• v.96 no.5
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• pp.551-560
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• 2007

We investigated physiological damages and biochemical alleviation of five species of genus Acer under ozone fumigation in order to assess their tolerant ability against ozone toxicity. At the end of 150 ppb $O_3$ fumigation, photosynthetic characteristics were measured, and chlorophyll contents, malondialdehyde (MDA) and antioxidative enzyme activities were analyzed in the leaves of five maple trees (Acer buergerianum, A. ginnala, A. mono, A. palmatum, and A. palmatum var. sanguineum). The reduction of chlorophyll (chl) a in ozone-exposed plants was 16.8% (A. buergerianum) to 26.7% (A. ginnala) of control plants. For the content of chi b, A. ginnala and A. palmatum var. sanguineum represented the high reduction of 26.3% and 23.6%, respectively. The highest reduction on the chi a:b ratio was observed in the leaves of A. palmatum. The reduction of net photosynthesis in five species varied from 2.4% to 37.6%. Among five species, A. ginnala showed remarkable reduction (37.6%) for net photosynthesis in comparison with control. Carboxylation efficiency differed significantly (P < 0.05) among species and between control and ozone treatment. The reduction of carboxylation efficiency was the highest in the leaves of A. ginnala (44.7%). A. palmatum var. sanguineum showed the highest increase (41.7%) for MDA content. The highest increase of superoxide dismutase (SOD) activity represented in A. palmatum (26.1%) and the increase of ascorbate peroxidase (APX) activity ranged from 16.5% (A. ginnala) to 49.1% (A. palmatum var. sanguineum). A. mono showed the highest increase (376.6%) of glutathione reductase (GR) activity under ozone fumigation and A. buergerianum also represented high increase (42.3%) of GR activity. Catalse (CAT) activity increased in the leaves of A. ginnala, A. palmatun and A. palmatum var. sanguineum under ozone exposure, whereas A. buergerianum and A. mono decreased in comparison with control plants. In conclusion, physiological markers such as chlorophyll content and photosynthesis that responded sensitively to $O_3$ in maple trees were considered as the very important indicators in order to evaluate the tolerance against $O_3$ stress, and parameters were closely related with each other. Among anti oxidative enzymes, SOD and APX might be contributed to alleviate to $O_3$ toxicity through the increase of activity in all maple trees. Therefore, these compounds can be used as a biochemical maker to assess the stress tolerance to $O_3$.