• Advances in nano research
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• 제3권4호
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• pp.243-255
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• 2015

In this investigation, ecotoxicity of nano and micro metal oxides, namely silica ($SiO_2$) and alumina ($Al_2O_3$), on the growth of green algae (Porphyridium aerugineum Geitler) is discussed. Effects of nano and micro particles on the growth, chlorophyll content and protein content of algae are analysed using standard protocols. Results indicate that $SiO_2$ nano and micro $SiO_2$ particles are non-toxic to P. aerugineum Geitler up to a concentration of 1000 mg/L. In addition, $Al_2O_3$ microparticles are less toxic to P. aerugineum Geitler, whereas $Al_2O_3$ nanoparticles are found to be highly toxic at 1000 mg/L. Moreover, $Al_2O_3$ nanoparticles decrease the growth, chlorophyll content, and protein content of tested algae. In addition, zeta potential and contact angle are also important in enhancing the toxicity of metal oxide nanoparticles in aquatic environment. This study highlights a new insight into toxicity evaluation of nanoparticles on beneficial aquatic organisms such as algae.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1989년도 추계 학술대회지
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• pp.42-43
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• 1989

• 한국식품조리과학회지
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• 제1권1호
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• pp.27-32
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• 1985

배추를 가열 처리했을 때와 친치를 제조하여 산발효시켰을 때에 시간의 경과에 따른 chlorophylls의 변화와 pH의 변화를 조사하였다. 가열했을 때에는 pheophytins의 생성이 뚜렷했고, 이것은 pH의 감소경향과 관계있으며, 1분간 가열했을 경우에는 chlorophyllase의 활성화에 의해 chlorophyllides의 생성이 촉진되었다. 김치의 경우는 숙성초기에 pH변화에 따라 chlorophylls의 함량이 급격히 감소했으며, 숙성말기에는 pheophytins와 pheophorbiaes만 존재했고, 가열한 경우에 비해 pheophorbides의 생성이 뚜렷했지만 pheophytins보다는 낮은 수준이었다.

• 한국미생물·생명공학회지
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• 제43권3호
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• pp.275-279
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• 2015

본 연구는 Chlorella vulgaris에 자외선을 조사하여 획득한 변이주 중 색소함량이 높은 변이주 2종(UBM1-2, UBM2-57)의 특성을 분석하였다. 2종의 변이주의 생장속도는 WT와 비교했을 때 20% 가량 더딘 생장율을 보였고, 세포건조 중량 및 단백질 함량 또한 세포생장과 비슷한 패턴을 보였다. 지질 함량은 UBM1-2 변이주에서 WT와 비교 시 21% 높은 지질 함량을 확인하였지만, UBM2-57 변이주는 WT보다 39% 낮은 지질함량을 축적하고 있음을 확인하였다. 하지만, 색소함량(클로로필, 카로티노이드)의 경우 WT와 비교 시 유의적으로 높은 함량을 축적하고 있음을 확인하였다. 클로로필함량은 UBM1-2, UBM2-57 변이주가 WT보다 각각 37%, 89% 높았고, 카로티노이드 함량은 WT보다 각각 27%, 70% 높음을 확인하였다. 본 연구를 통해 선별한 미세조류 변이주는 야생균주와 비교 시 세포 생장에 큰 차이를 보이지 않으며, 유용성분을 다량 함유하고 있어 화장품 및 의약품, 건강 기능식품의 중요한 소재로써 이용이 가능할 것으로 판단된다.

• 한국식품영양과학회지
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• 제30권3호
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• pp.377-381
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• 2001

Chlorophyll a and b, pheophytin a and b and $\beta$-carotene, crude chlorophylls and carotenoids (CCC) extracts of mustard leaf kimchi were isolated by DEAE-sepharose CL-6B and Sepharose CL-6B colume and TLC. The effects of chlorophyll a and b, pheophytin a and b and $\beta$-carotene on linoleic acid autoxidation were examined by the determination of peroxide value and conjugated dienoic acid content. Among them, chlorophyll a showed greater antioxidative activity than others, followed by chlorophyll b, pheophytin a, pheophytin b and $\beta$-carotene. Degradation of pheophytin b was observed to be slower than others and $\beta$-carotene showed highest degradation level during the autoxidation reaction of linoleic acid.

• 한국식품과학회지
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• 제47권5호
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• pp.561-566
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• 2015

350 ppm 농도로 물/카놀라 기름 에멀션에 첨가된 달걀 노른자 위 레시틴은 에멀션 기름의 철 이온에 의한 자동산화와 클로로필에 의한 감광산화에 유의한 영향을 나타내지 않았다. 에멀션의 산화 중 달걀 노른자위 레시틴으로부터 유래한 인지방질은 분해되었으며 감광산화에 비해 자동산화에서 분해 속도가 높았다. 그러나 PC와 PE 사이의 분해 속도는 두 종류의 산화에서 모두 유의한 차이를 보이지 않았다(p>0.05).

• Journal of Biosystems Engineering
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• 제25권6호
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• pp.517-526
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• 2000

A series of experiments was conducted to evaluate the capability of detecting nutrimental deficient stress of N, P and Ca of a tomato plant using several fast and intact type physiological properties measuring devices - a chlorophyll content meter an infra-red thermometer to measure leaf temperature a chlorophyll fluorescence meter a porometer an optical spectrometer a multi-scan radiometer and a canopy analyzer. to detect N deficient stress a chlorophyll content meter a spectrometer and a multi-scan radiometer were useful and their possibility to detect was estimated as about 50%, 100% and 100% respectively. To detect P deficient stress the infra-red thermometer the porometer and the spectrometer proved their usefulness an their possibility to detect was estimated as about 70%, 70% and 70% respectively. To detect Ca deficient stress an thermometer a porometer a spectrometer and a multi-scan radiometer were useful and their possibility to detect was estimated as about 60%, 70%,80% and 100% respectively. The experiments resulted that use of a spectrometer and a multi-scan radiometer in combination with a chlorophyll content meter an infra-red thermometer and a porometer were desirable to distinguish the nutrimental stress tested in the study.

• Journal of Plant Biology
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• 제27권3호
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• pp.139-147
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• 1984

This in vitro study was employed to clarify the effects of several pollutants i.e. $SO_2$, fluoride, cadmium(Cd), aluminum(Al) and NaCl, on the organogenesis and growth responses of shoot-tip, stem and multiple-buds segments derived from hypocotyl or cotyledon culture of petunia seedlings. ${Na_2}{SO_3}$levels of more than 200$\mu{g}$/ml had significantly reduced organogenesis, growth, and chlorophyll content. The injuries caused by ${Na_2}{SO_3}$, concentration of more than 400$\mu{g}$/ml were alleviated by increasing hydrogenion concentration of medium, indicating some relationship between two factors. Organogenesis was not affected by the fluoride concentration up to 100ppm in the media, but the growth and chlorophyll content were greatly reduced by the fluoride. The effect of Cd depended on the explant sources used for the culture; 1.0ppm was effective for fresh weight increase in shoot tip culture, and 3.0ppm in stem segments culture. Organogenesis and growth were greatly reduced by more than 10.0 Cd treatment. Growth and formation of shoots were better with Na conc. of 0.3% compared to control, but those of roots were inhibited. Na concentration goes over 1.0%, organogenesis and subsequent growth were inhibited, and chlorophyll synthesis was drastically reduced. Chlorophyll content was increased on the medium supplemented with Al 50$\mu{g}$/ml compared to control. However the formation and growth of shoots were greatly inhibited with more than 400$\mu{g}$/ml and roots were not produced at all.

• 한국자원식물학회지
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• 제27권6호
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• pp.629-634
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• 2014

Rice type tartary buckwheat is used as a substitute for rice in many Asian countries due to its easy dehulling character. The objective of the present study was to determine the chlorophyll, total polyphenol (TP), total flavonoid (TF), antioxidant activity and to quantify the bioactive compounds rutin, quercetin and chlorogenic acid in the seedlings of rice-type tartary buckwheat (RTTB). Young seedlings exhibited higher antioxidant (DPPH radical inhibition) activity in dose dependent manner. TP and TF content were highest ($3017.46{\pm}201.84{\mu}g$ TAE/100 mg dw and $1916.0{\pm}102.95{\mu}g$ QE/100 mg dw respectively) in 3 days after germination (DAG) seedlings compare to 6 and 9 DAG. The contents of rutin and quercetin increased with growing stage of seedlings. However, the chlorogenic acid decreased with increasing growth. Overall, RTTB seedlings can be regarded as a strong source of phenolics and have high possibility for food and nutraceutical application due to their efficient antioxidant properties, higher chlorophyll and phytochemical content.

• 한국식품영양과학회지
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• 제27권5호
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• pp.852-857
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• 1998

Three kinds of kimchi using Chinese cabbage, leafy radish and mustard leaf were prepared by conventional method and stored at 5$^{\circ}C$ or 2$0^{\circ}C$ for 13 dyas. During storage at both temperatures, changes of the amounts of salt and ascorbic acid, pH and total acidity were determined, and the relationship of the decomposition of chlorophylls with the production of their derivaties was studied. At both storage temperatures, salt concentration of Chinese cabbage kimchi(3.7%), leafy radish kimchi(3.6%), mustard leaf kimchi(3.5%) was relatively constant during the entire storage period. However, pH and total acidity wre fluctuating with the remarkable changes during 3 days of storage. Ascorbic acid content was slowly decreased during the storage period and the decompositin rate of ascorbic and was greater at 2$0^{\circ}C$ than 5$^{\circ}C$. Among the kinds of kimchi tested, mustard leaf kimchi with the slow decomposition rate of ascorbic acid contained relatively high ascorbic acid content, while leafy radish kimchi contained the lowest content. At both storage temperatures, the production of pheophytin and pheophorbide from decomposition of chlorophyll was least in mustard leaf kimchi, but similar production rates in leafy radish and Chinese cabbage kimchi were observed.