• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.609-615
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• 2011

Forsythia saxatilis is a Korean endemic plant designated as rare and endangered by the Korea Forest Service (KFS). Growth and physiological characteristics of F. saxatilis were investigated under two different light intensities in order to figure out an appropriate growth environment for conservation and restoration of the species in its natural habitat. Shoot length, leaf size and weight, photosynthetic pigment content and photosynthetic parameters were measured for F. saxatilis grown at two experimental plots under relative light intensities (RLI) of 20% and 60% of the full sun, respectively. Fresh leaf weight of plants grown under high relative light intensities (RLI-60) exceeded that of plants grown at 20% RLI. The ratio of fresh leaf weight to leaf size at RLI-60 was 1.47 times superior comparing to that recorded at RLI-20. The content of photosynthetic pigments such as chlorophyll a, b and carotenoid were higher in plants grown at RLI-60, whereas the ratio of total chlorophyll to carotenoid content was higher in the leaves at RLI-20. Photosynthetic rate, stomatal conductance and transpiration rate at RLI-60 were, respectively, 2.5, 2.65 and 1.79 times higher comparing to those recorded at RLI-20. Water use efficiency, however, was higher at RLI-20. The chlorophyll/nitrogen ratio was 1.83 times higher at RLI-20 than at RLI-60. In contrast, the ratio of net photosynthesis to chlorophyll content at RLI-60 was 2.58 times higher than that of RLI-20. In conclusion, light intensity might be the major factor affecting growth and physiological characteristics of F. saxatilis grown under canopy of tall tree species.

• Plant Biotechnology Reports
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• v.2 no.3
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• pp.207-212
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• 2008

Plantlets of Alocasia amazonica regenerated under a photon flux density (PFD) of 15 or $30{\mu}mol\;m^{-2}s^{-1}$ showed better growth and development than those grown under higher PFDs. While chlorophyll a and chlorophyll b decreased, the number of stomata increased with increasing PFD. Photoperiods also affected plantlet growth and stomatal development. Highest growth was observed for the short photoperiod (8/16 h) and for equinoctial (12/12 h) light and dark periods. Very few stomata developed in the leaves of plantlets grown under a short photoperiod (8/16 h) and the number of stomata increased with increasing light period. In conclusion, both light intensity and photoperiod independently affect growth of A. amazonica and development of stomata, depending on the intensity and duration of light treatment.

• Journal of Plant Biology
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• v.31 no.3
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• pp.227-237
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• 1988

Effects of putrescine on senescence in detached leaves of Chinese cabbage (Brassica campestris L.) in the light were investigated. The putrescine as a potent antisenescence substane markedly inhibited chlorophyll and protein loss at the 10mM concentraton in the detached leaves during the dark incubation. In the light, however, putrescine showed the opposite effects to dark incubation. The chlorphyll loss by putrescine in the light was stopped with darktransfer, and inhibited competitively by a divalent cation Ca2+. In the light, putrescine reduced the protease activity. Putrescine, in the light, increased H2O2 content and reduced the activities of enzymes -superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6-involved in inhibiting the accumulation of free radicals. These results suggest that the effects of puterscine on chlorophyll and protein loss in detached leaves of Chinese cabbage in the light are related to the cationic nature of putrescine and the accumulation of free radicals.

• Journal of Ecology and Environment
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• v.40 no.2
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• pp.120-124
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• 2016

Background: Although cetrimonium bromide is widely used for its bactericidal effects, the safety of cetrimonium bromide remains controversial. Therefore, the phytotoxicity of cetrimonium bromide was tested to evaluate its acute toxicity to plants and possible toxicity to other organisms and the ecosystem. Results: The germination rates of two test species, Lactuca sativa and Brassica campestris, were significantly decreased after cetrimonium bromide treatment. Furthermore, cetrimonium bromide treatment at over 1 mg/L concentration significantly affected root elongation immediately after germination. In pot experiments with semi-mature plants, significantly decreased shoot elongation and chlorophyll content were detected in both species following cetrimonium bromide treatment. Cetrimonium bromide treatment also significantly increased the antioxidant enzyme activities of plants. Conclusion: Our results show that cetrimonium bromide is phytotoxic, and since phytotoxicity testing can imply potential toxicity in the environment, further studies of the environmental toxicity of cetrimonium bromide should be performed.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.113.1-113.1
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• 2011

미세조류 내의 엽록소는 바이오디젤 전환 반응에서 산 촉매의 활성을 억제 할 뿐만 아니라, 짙은 색상을 띄게하여 바이오디젤 품질규격으로부터 벗어나게 한다. 미세조류의 엽록소 분석은 용매에 의해 엽록소를 추출한 후, 흡광도를 측정하여 그 함량을 계산하는 방법을 널리 사용하고 있다. 건조된 미세조류의 분석은 선택되는 용매에 따라 최대 추출량이 달라지는 것을 제외하고 큰 문제가 없지만 미세조류를 lipid 오일로 변환하면, 용매에 녹지 않아 추출이 되지않는 문제가 발생하여 흡광도 측정을 어렵게 한다. 따라서 미세조류의 형태가 powder일 때와 오일인 경우를 구분하여 용매를 선택해야 하며, 오일 또는 powder 형태 구분 없이 사용할 수 있는 분석법을 적용하여 서로 다른 엽록소 함량을 비교한 후 분석법 간의 상호 장단점을 파악해야한다. 본 연구에서는 메탄올을 용매로 사용하는 분석법(porra et al.)과 아세톤을 용매로 사용하는 분석법(Humphrey and Jeffrey)을 적용하여 엽록소 함량을 비교하였고, AAS(Atomic Absorption Spectrometer)를 통한 Mg 함량 측정을 통해 엽록소 함량을 계산하는 분석법간의 차이를 확인하였다.

• Journal of Plant Biology
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• v.27 no.4
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• pp.241-251
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• 1984

The present study investigated the effects of triacontanol (TRIA) on plant growth and peroxisomal enzyme activities in radish seedlings. The optimum concentration of TRIA with respect to radish seedling bioassay was decided to 1.0mg $1^{-1}$. In comparison to untreated controls (including Tween 20 treatment), 1.0mg $1^{-1}$ TRIA treatment caused an increase in seed germination rate and root growth, but no stimulation in hypocotyl growth. Chlorophyll accumulation in cotyledon during carly development stage increased rapidly, and degradation of chlorophyll in later stage due to the cotyledon senesence was noticeably retarded. Increase of soluble protein content in cotyledon at early period of development was observed. Isocitrate lyase and catalase activity was not significantly different in both the treated and the untreated plants. But, glycolate oxidase activity was inhibited by TRIA down to 20% against controls. Also, the increase of the activity of peroxidase, a leaf-senescence marker enzyme, was continuously retarded over control for 8 days of development. Based on above results, TRIA was found to be active in both the growth and the peroxisomal enzyme activities of radish seedlings.

• Journal of Plant Biology
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• v.31 no.2
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• pp.155-164
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• 1988

The rapid senescence of detached Chinese cabbage leaf discs in darkness is first manifested by a sharp rise in malondialdehyde content (indicated by distruption of membrane structure), then by a rise in peroxidase activity and a decrease in catalase activity, and ultimately by chlorophyll degradation. These changes in parameters besides the catalase activity during senescence were delayed by application of spermidine. Especially, 10-4M spermidine almost completely arrested chlorophyll degradation after incubaton over 5 days. Spermidine reduced the amount of ethylene produced by senescing leaf discs. Additionally, it also reduced IAA-induced ethylene production. Calcium ion (1mM, 10mM) supplied together with the spermidine diminished the spermidine action, indicating probable involvement of an initial ionic attachment mechanism. These results suggest that spermidine can be used as an anti-senescence agent for plants and that this agent may stabilize membrane structure through interaction with the negatively charged loci on the membrane and exert the influence during senescence.

• Asian Journal of Turfgrass Science
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• v.9 no.1
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• pp.43-51
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• 1995

This study was conducted from September 1, 1994 to December 12, 1994 to investigate the leaf color characteristics, flowering period and fruit period of Wild plants in Suwon and Taejon area. The results were as follows; 1. By using the chlorophyll meter, the chlorophyll content of Liriope platyphylla, Trifolium repens, Dystaenia takeshimana was 30.0, 22, 2 and 19.0, respectively on December 9, 1994. 2. By leaf color based on KBS standard color number, Liriope piatyphylla, Trifolium repens, Dvstoenia takeshirnana were green till December 9, 1994. 3. The flowers of Aster sea ber, Atractylodes japonica, Allium thunbergii, Liriope platyphylla, Scilla scilloides, Digitaria sanguinalis, Sanguisorba officinalis were persisting till September, and the flowers of Crysanthemum boreale were persisted till November and Allium thunbergii, till October.

• Applied Chemistry for Engineering
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• v.26 no.4
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• pp.421-426
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• 2015

Arthrospira platensis (A. platensis) has been used in various fields including dietary supplements as it contains a high protein content and large amounts of unsaturated fatty acids. In addition, it has some pigments such as phycocyanin, myxoxanthophyll and zeaxanthin and thus has been used as a food additive and antioxidant substance. Nano-bubble hydrogen is to dissolve more than the saturation solubility in water by injecting the hydrogen gas in the nano-bubble hydrogen water. The nano-bubbles are known to possess higher antioxidant properties in addition to anticancer effects. In this paper, Arthrospira platensis was cultured in both a normal medium with distilled water and nano-bubble hydrogen water medium and their properties were compared. The cell growth and the content of chlorophyll and carotenoid in the nano-bubble hydrogen water was 15% higher than that of the control. The level of phycocyanin in nano-bubble hydrogen water was also 7% higher than that of the control. However, there were little differences in the lipid content between the nano-bubble and control. To determine the content of the antioxidants, the level of flavonoid and polyphenol were measured. The level of flavonoid in nano-bubble hydrogen water was found to be more than 70% increased when comparing to that of the control, while the level of polyphenol was similar to each other.

• Weed & Turfgrass Science
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• v.7 no.1
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• pp.62-71
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• 2018

This study was conducted to evaluate the effect of liquid fertilizer containing humate (LFH) on changes of turfgrass quality and growth by investigating visual quality, chlorophyll content, dry weight of clipping, and nutrient content in leaves tissue. Treatments were designed as follows; control fertilizer (CF), HF-1 ($CF+1.0mL\;m^{-2}\;LFH$), HF-2 ($CF+2.0mL\;m^{-2}\;LFH$), and HF-3 ($CF+4.0mL\;m^{-2}\;LFH$). As compared with CF, soil chemical properties of LFH treatments were not significantly. Visual quality and root dry weight of LFH treatments were higher than that of CF. Chlorophyll content, clipping yield and nitrogen uptake of HF-2 and HF-3 were increased 11.2-11.8%, 15.3-30.0%, 22-42% by application of LFH. The LFH level was positively correlated with visual quality, chlorophyll content, clipping yield or nutrient uptake amount. These results indicated that the application of LFH improved the growth and quality of creeping bentgrass by increasing nutrient uptake and by prompting root growth.