• Journal of the East Asian Society of Dietary Life
• /
• v.14 no.2
• /
• pp.156-160
• /
• 2004

This study was carried out to examine the changes of chlorophyll a and b contents by the extraction temperatures and the number of soaking using five kinds of Korean green teas(3 kinds of green tea and 2 kinds of tea bag) which were obtained from a local market in Daegu city, Korea. The chlorophylls were extracted with 80% acetone, and determined by a visible spectrophotometer. The concentration of chlorophyll in Sulrok(tea bag) were the highest among all kinds of teas when they were extracted at 60, and decreased with the number of soaking increased. On the other hand, the chlorophyll content of green teas was the highest at 80, and decreased with the increase of the number of soaking as the tea bag did. It was found that the Korean tea bag contained more chlorophyll than green teas did, and green teas contained significantly more chlorophyll b than chlorophyll a.

• The Korean Journal of Ecology
• /
• v.18 no.1
• /
• pp.137-146
• /
• 1995

Acer pseudo-sieboldianum, A. ginnala, A. negundo and A. saccharinum were selected as materials for the studies on the seasonal fluctuation of chlorophyll content and photosynthetic rates. In all the four species during the growing season except in October, the principal component that determined the total chlorophyll content was chlorophyll a. Content of chlorophyll b increased with leaf age, but that of chlorophyll a decreased. In contrast to A. saccharinum and A. pseudo-sieboldianum which showed their maximum chlorophyll content in June, A. negundo, which showed the highest chlorophyll content of the four species, exhibited its maximum chlorophyll content in July, whereas September in the case of A. ginnala. The fluctuation of chlorophyll content was similar to that of air temperature, and it increased till July. But chloprophyll content showed a significant relationship in early stage of leaf development, and there could be any interdependence between them in accordance with the seasonal change, no longer. In all four species light compensation points decreased in accordance with the seasonal change, and the maximum photosynthetic rates were obtained in August. Respiratory rates were shown no significant difference among species, and they decreased according to the adbance of season.

• Korean Journal of Soil Science and Fertilizer
• /
• v.31 no.1
• /
• pp.56-60
• /
• 1998

Radish and Chinese cabbage were cultivated under hydrophonic culture to investigate the effect of chromium on germination, cell elongation, ${\alpha}$-amlyase activity, contents of chlorophyll and protein. With increasing concentration of cromium, germination, cell elongation, ${\alpha}$-amlyase activity were decreased in both radish and Chinese cabbage, the rate was higher in radish than in Chinese cabbage. Contents of chlorophyll a and b were also decreased and chlorophyll a was higher than chlorophyll b. As the concentration of chromium was increased inhibited in the order of protein> ${\alpha}$-amylase activity>chlorophyll a>chlorophyll b.

• Proceedings of the KSRS Conference
• /
• 2002.10a
• /
• pp.208-208
• /
• 2002

Relationship between chlorophyll a in the East China Sea and spectral bands (412, 443,490, (510), 555, (676,765) in) of OSMI (Ocean Scanning Multi-Spectral Imager) including the profile multi-spectral radiometer (PRR-800) was studied. The values of remote sensing reflectance (Rrs) at the bands corresponding to the field chlorophyll a in α in the East China Sea were much higher than those in clear waters off California, USA. In case of the particle absorptions related to the chlorophyll a concentration at the spectral bands (440, 670 nm) were much higher in the East China Sea than the ones in the clean waters off California. The normalized water leaving radiances (nLw) at 412, 443, 490, 555 m of OSMI and field chlorophyll a in the East China Sea were correlated each other. According to the results, the relationship between field chlorophyll a and nLw 410 m in OSMI bands was the lowest, whereas that between the field chlorophyll a and nLw 555 nm in the bands was the highest. Reciprocal action between the field chlorophyll a and the band ratio of the OSMI bands (nLw410/nLw555, nLw443/nLw555, nLw490/nLw555) was also studied. Correlation between the chlorophyll a and the band ratio (nLw490/nLw555) was highest in the OSMI bands. Relationship between the chlorophyll a and the ratio (nLw443/nLw555) was higher than one in the nLw410/nLw555. The difference in the estimated chlorophyll α (mg/m3) between OSMI and SeaWiFS (Sea Viewing Wide Field-of-View Sensor) at the special observing stations in the northern eastern sea of Jeju Island in february 25, 2002 was about less than 0.3 mg/m3 within 3 hours. It is suggested that OC2 (ocean color chlorophyll 2 algorithm) be used to get much better estimation of chlorophyll α from OSMI than the ones from the updated algorithms as OC4.

• Journal of Biosystems Engineering
• /
• v.28 no.3
• /
• pp.245-252
• /
• 2003

This study was investigated the possibility of measuring chlorophyll in paddy crops using two kinds of chlorophyll meters(SPAD-502, Field Scout). The results of correlation analysis showed the 0.7758(r$^2$) relationship between the chlorophyll contents of paddy leaves measured by SPAD and spectrophotometer. It indicates that chlorophyll content in paddy crops could be measured using the SPAD. Considering the data of the SPAD and Field Scout there was significant difference between their chlorophyll contents measured by the SPAD and Field Scout. Likewise, such results were consistent with the corrected data using light intensity. According to the results, it can be concluded that it is difficult to describe the rice chlorophyll measured by the Field Scout more accurately than the SPAD when using one standard spectrum and another spectrum for reflectance measuring. The chlorophyll variance measured at a research institute and a farmer's field revealed that the output of SPAD was more reliable than that of the Field Scout.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.37 no.3
• /
• pp.413-423
• /
• 2013

Chlorophyll is an abundant pigment found in all green plants, algae, and cyanobacteria. This study uses methanol, acetone and water to extract spinach and mate powders in order to examine the possibility of dyeing animal fibers with chlorophyll without chemical alteration. It was shown that methanol extracts of spinach and mate powders can be effectively used to dye wool and silk fabrics if the extract is mixed with water by methanol:water 65:35 v/v. Compared to methanol extract, the acetone extract showed lower chlorophyll yield and lower dye uptake. Water was not an appropriate solvent for chlorophyll extraction and dyeing. Spinach powder showed a higher dye uptake than mate powder due to the higher chlorophyll content than mate powder. It is possible that the chlorophyll dyeing of wool and silk fabrics is due to the hydrogen bonding between the hydroxy amino acids in fiber and the carbonyl groups of chlorophyll. These carbonyl groups are on the heterocyclic ring and the methyl and ethyl side chains of chlorophyll.

• Asian Journal of Turfgrass Science
• /
• v.4 no.1
• /
• pp.12-23
• /
• 1990

The chlorophyll-protein complexes were separated from thylakoid membranes of Spinach oleracea and Zoysia japonica by two gel Systems of LiDodSO4-PAGE and LiDodSo4/Urea- PAGE under nondenaturing conditions. Seven chlorophyll~protein complexes of CPI*, CPI, CPII*. CP47, CP43, CP29 and CPII were fractionated from both S,oleracea and Zjaponica by LiDodSO4-PAGE. CPI, CP47 and CP43 contained more chlorophyll a than chlorophyll b. The patterns of their absorption spectra at room temperature were similliar to that of chlorophyll a, judging by their UV-spedtroscopy. On the other hand, CPII* and CPII contained approximately equim-olar quantities of chlorophyll a and b. Additional five chlorophyll-protein complexes not separated in the LiDodSO4-PAGE system were electrophoretically isolated from both S, oleracea and Zjaponica by LiDodSO4/Urca-PAGE. The chlorophyll-protein complex just above LRCII $\alpha$in the gel appears CCII-RC separeted recently. 23 kDa and 20 kDa cho-protein complexes is probably LHCIa and LHCIb as judged from their molecular weight. Two novel chlorophyll~protein complexes designated "CPI7" and "CPI6" were fractionate by this gel system. Their molecular weights respectively. Although the stoichiometry of their components and their roles in thylakoid membranes are not apparant, It is thought that they are another kinds of LHCI.other kinds of LHCI.

• Journal of Plant Biology
• /
• v.7 no.4
• /
• pp.1-8
• /
• 1964

This experiment was investigated from March 20 to September 20, 1960. It was investigated chlorophyll, carotene and ascorbic acid contents of edible plants in Korea, 64 with 132 species belonging to 62 families for the ascorbic acid and 116 species on the 64 families for the chlorophyll and carotene content. Carotene content varies with chlorophyll although the ratio of chlorophyll to carotene was not so high as that obtained by Beak and Redman (1). Where the chlorophyll content is abundant, it appears that the amounts of carotene and ascorbic acid are also plenty.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.23 no.6
• /
• pp.954-958
• /
• 1994

The bleaching effect of chlorophyll a by two lipoxygenase isoenzymes (LOX-1, LOX-2) isolated from potato tuber(variety DEinma ) was studied. In the presence of LOX-1 or LOX0-2 with linoleic acid chlorophyll a bleaching occurred during two isoenzymes-mediated oxidation of linoleic acid. Chlorophyll a bleaching porceeded with decreasing in the formation of conjugated dienes form linoleic acidyb LOX-1 and LOX-2 . In the presence of chlorophyll a, LOX-2 showed a markable decrease inproduction of conjugated dienes from linoleic acid and a higher chlorophyll a bleaching activity. compared with LOX-1. These results suggest chlorophyll-bleaching reaction required intermediates formed during the peroxidation of linoleic acid by lipoxygenase isoenzymes, thus preventing formation of conjugated dienes.

• Journal of Environmental Science International
• /
• v.14 no.10
• /
• pp.911-917
• /
• 2005

A comparison between the estimated chlorophyll a from OSMI, the SeaWiFS and the chlorophyll a measured from the research cruises of National Fisheries Research and Development Institute was made. The updated empirical algorithm for calibrating and validating of the estimated chlorophyll a in the East China Sea was formulated by relationship between the estimated chlorophyll a and the field one. The relationship between the chlorophyll a and the band ratio(nLw490/555) was still highest in the OSMI data after launching of KOMPSAT satellite. The distributions of OSMI chlorophyll a were compared with those of sea surface temperature, zooplankton biomass, and catch amounts of the Pacific mackerel in the East China Sea. In case of the relationships in specially winter seasons of 2002 and 2004, the zooplankton and the fish were totally depended on the distributions of SST than those of chlorophyll a.