• Korean Chemical Engineering Research
• /
• 제55권1호
• /
• pp.74-79
• /
• 2017

본 연구에서는 Chlorella saccharophila의 배양을 통하여 바이오에너지 자원을 대량으로 확보하고자 배지 최적화 실험을 진행하였다. 최적화 인자로는 배양 형태, 초기 접종량, 탄소원 종류 및 농도, 질소원 종류 및 농도, 배양시간이다. 실험 결과, 배양 형태는 광원과 외부탄소원을 모두 공급하는 mixotrophic 배양이 적절하였다. 초기 접종량은 3% (v/v), 탄소원은 glucose 30 g/L, 질소원은 $NaNO_3$ 0.95 g/L를 첨가하는 것이 우수하였다. 최적 배지 조건으로 배양한 결과, oil의 함량은 12일에서 가장 높았으나, 회수되는 C. saccharophila의 biomass양과 chlorophyll의 양은 10일에서 가장 높았다. 위의 결과는 미세조류의 배지 최적화를 통하여 대량배양을 위한 기초자료로 사용될 수 있으리라 판단된다.

• 한국미생물·생명공학회지
• /
• 제44권4호
• /
• pp.522-528
• /
• 2016

본 연구에서는 3종류의 목질계 바이오매스와 4종류의 해조류 바이오매스를 전처리와 효소가수분해 과정을 통하여 얻은 환원당을 이용하여 C. saccharophila를 배양하였다. 얻어진 가수분해물을 활성탄 처리 유무에 따라 배양한 결과, 목질계 바이오매스와 해조류 바이오매스 가수분해물을 활성탄 처리하지 않은 경우에는 C. saccharophila의 성장이 저해되었다. 목질계 바이오매스인 유채대와 보리대 그리고 볏짚과 해조류 바이오매스인 미역, 다시마, 파래 그리고 꼬시래기 가수분해물의 경우 활성탄 등으로 처리한 경우에서 목질계 바이오매스와 해조류 바이오매스 가수분해물에서 C. saccharophila의 성장이 관찰되었다. 미세조류의 생산성과 oil 함량, chlorophyll 등을 고려하였을 때 목질계 바이오매스와 해조류 바이오매스는 C. saccharophila의 product 생산을 위한 배양에 이점을 가지며, 특히 chlorophyll 생산에 보다 유용하리라 판단된다.

• ALGAE
• /
• 제31권3호
• /
• pp.267-276
• /
• 2016

Microalgae are currently a very promising source of biomass and triacylglycerol (TAG) for biofuels. In a previous study, we identified Chlorella saccharophila as a suitable source of oil for biodiesel production because it showed high biomass and lipid content with an appropriate fatty acid methyl esters profile. To improve the TAG accumulation in C. saccharophila, in this study we evaluated the effect of abscisic acid (ABA) addition on cell concentration, lipid content and TAG production in this microalga. First, we evaluated the effects of four ABA concentrations (1, 4, 10, and 20 μM) added at the beginning of a single-stage cultivation strategy, and found that all concentrations tested significantly increased cell concentration and TAG content in C. saccharophila. We then evaluated the addition of 1 μM ABA during the second stage of a two-stage cultivation strategy and compared it with a nitrogen deficiency treatment (ND) and a combination of ND and ABA (ND + ABA). Although ABA alone significantly increased lipid and TAG contents compared with the control, ND showed significantly higher TAG content, and ND + ABA showed the highest TAG content. When comparing the results of both strategies, we found a superior response in terms of TAG accumulation with the addition of 1 μM ABA at the beginning of a single-stage cultivation system. This strategy is a simple and effective way to improve the TAG content in C. saccharophila and probably other microalgae as a feedstock for biodiesel production.

• Fisheries and Aquatic Sciences
• /
• 제15권2호
• /
• pp.125-135
• /
• 2012

This study was carried out to understand the correlation between phylogenetic relationships based on 18S rDNA sequences and growth by salinity of Chlorella-like species. The 18S rDNA sequences of 71 Chlorella-like species which were mainly collected from Korean waters were analyzed. The 18S rDNA sequences of Chlorella-like species were divided into three groups (group A, B and C) and group B was further divided into three subgroups (subgroup B-1, B-2 and B-3). Thirty-seven Chlorella-like species in group A grew well at high salinity (32 psu) but the other groups grew well in freshwater. The sequence identities of the species in group A and B were 97.2-99.5%, but those of 6 species in group C ("Chlorella" saccharophila), which contained group I intron sequences region were 75.0-75.4%. Two representative species of each group were cultured at different salinities (0, 16 and 32 psu) to examine the correlation between the molecular phylogenetic groups and the phenotypic characteristics on cell growth and size by different salinities. The size of cell cultured at different salinities varied according to the species of each molecular phylogenetic group. The size of "Chlorella" saccharophila in group C was bigger and more obviously elliptical rather than that of the other Chlorella-like species. Considering the results on molecular and phenotypic characteristics, the group A and B belonged to Chlorellaceae, but group C was distinctly different from them.