• 아시안잔디학회지
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• 제20권2호
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• pp.167-174
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• 2006

키토산 처리에 의한 크리핑 벤트그래스의 생장에 미치는 영향에 대하여 분석하였다. 엽장은 무처리 평균은 25.1cm로 확인되었고, 키토산 500배 처리 시 평균 31.6cm로 가장 높은 생육증가를 나타내었다. 엽수는 무처리 시 평균은 27.9매로 나타났고, 키토산 300배 처리 시 45.5 매로 증가하였다. 뿌리길이는 무처리에서 21.1cm인 것으로 나타났으나, 키토산 600배 처리 시 27.9cm로 뿌리 길이의 생육이 증가하는 것으로 나타났다. 엽록소 함량은 무처리 시에서 $11.9mg/100cm^2$로 나타났고, 키토산 300배 처리에서 $18.4mg/100cm^2$로 최대 증가를 나타내었다. 뿌리길이의 생장, 엽록소 함량, 지상부 생체중과 건물중, 지하부 생체중과 건물중은 키토산 500배 처리 농도에서 가장 높은 생장 특성을 나타냈다.

• Applied Biological Chemistry
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• 제43권1호
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• pp.34-38
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• 2000

과채류에 대한 키토산 유기농업의 효과를 알아보기 위한 시도의 일환으로 배추의 생장 및 배추 중의 ${\gamma}-aminobutyric$ acid(GABA) 함량에 미치는 키토산비료의 시비효과를 조사하였다. 육묘상태에서 대조구에는 물을, 실험구에는 3% 키토산액을 500배 희석하여 2일에 1회 처리하였고, 본 밭에 정식후 대조구에는 물을, 실험구에는 동일한 키토산 희석액을 7일 1회 간격으로 엽면시비 하였다. 키토산비료의 시비는 물 처리구에 비하여 육묘상태에서 배추묘의 잎 길이를 증가시켰고, 어린묘 중의 GABA함량도 증진시켰다. 키토산비료의 시비는 본밭 토양의 물리화학적 특성을 크게 변화 시키지 않으면서 본밭에서의 배추의 평균 둘레 및 무게를 대조구에 비하여 증가시켰으며, GABA 함량도 증진시키는 것으로 조사되었다. 이들 결과들은 키토산비료의 시비가 배추의 생산량과 품질을 일부 향상시킬 수 있음을 제안해 주는 것이다.

• 한국자원식물학회지
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• 제17권1호
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• pp.48-53
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• 2004

인삼 부정근의 생장과 ginsenosides 함량에 미치는 elicitor의 영향을 조사한 결과 chitosan처리구의 생장량은 10 mg/L에서 가장 양호하였으며, ginsenosides의 함량은 5 mg/L처리농도에서 14.48 mg/g $.$ DW로 대조구에 비해 약9%의 함량증대를 가져왔다. 세포내에서 이차대사계를 활성화시키는 중간 신호전달 물질(signal transducer)로 알려진 jasmonic acid를 인삼 부정근 배양에 처리한 결과 부정근의 생장량은 10 uM 처리구에서 ginsenosides함량이 가장 양호하였으나, 인삼 부정근의 생장은 다소 억제되었다.

• Environmental Analysis Health and Toxicology
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• 제22권3호
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• pp.279-285
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• 2007

The present study attempted to analyze the mechanism of PCB-induced neurotoxicity with respect to the PKC signaling. Since the developing neuron is particularly sensitive to PCB-induced neurotoxicity, we isolated cerebellar granule cells derived from 7-day old SD rats and grew cells in culture for additional 7 days to mimic PND-14 conditions. Only non-coplanar PCBs at a high dose showed a significant increase of total PKC activity at $[^3H]PDBu$ binding assay, indicating that non-coplanar PCBs are more neuroactive than coplanar PCBs in neuronal cells. PKC isoforms were immunoblotted with respective monoclonal antibodies. PKC-alpha and-epsilon were activated with non-coplanar PCB exposure. The result suggests that coplanar PCBs have a PKC pathway different from non-coplanar PCBs. Activation of PKC with exposure was dampened with treatment of high molecular weight of chitosan. Chilean (M.W. > 1,000 kDa) inhibited the total activity of PKC induced by the non-coplanar PCBs. Translocation of PKC isoforms was also inhibited by the high molecular weight of chitosan. The study demonstrated that non-coplanar PCBs are more potent neurotoxic congeners than coplanar PCBs and the alteration of PKC activities by PCB exposure can be blocked with the treatment of chitosan. The results suggest a potential use of chitosan as a means of nutritional intervention to prevent the harmful effects of pollutant-derived diseases.

• 한국유기농업학회지
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• 제8권1호
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• pp.89-99
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• 1999

To do mass multiplication of plug seedlings in potatoes, apical stem cuttings originated from virus-free microtubers were cut to one-two internodes and transplanted into the plug-tray. After 10days, we applied Chitosan and Wood Extraction on rooting and tuber formation of plug seedlings. To improve field adaptability of plug seedlings, rooted cuttings with a height of 20cm after 20days of cutting were transplanted ito the fields, We applied 500~2000ppm Chitosan on growth characteristics and tuber formation of that. The above and underground growths, such as plant height and number of leaves were significantly more vigorous after treatment with 500~1,000ppm Chitosan and 2,000ppm Wood Extraction, the spray treatment was carried out five times at intervals of four days after ten days of transplanting. T-N, K, P, Mg and Na, were higher as the concentrations of chitosan and Wood Extraction were higher. The growth and tuber yield in plug seedlings planting plot and seed potatoes planting plot were effectively highter as foliar application of Chitosan(500~2,000ppm) was done after planting the plot. T-N content in leaves and tuber was higher as the concentration of Chitosan was high. A similar tendency was shown in K, P and Mg. In the small tuber(under 30g), the number of tubers and tuber yield were relatively increased in the seed potatoes planting plot, but the large tubers(over 80g) yield was higher in the plug seedlings planting plot, and in order to increase tuber yield in plug seedlings it was necessary to add plant density to the field.

• 한국유기농업학회지
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• 제4권2호
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• pp.79-85
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• 1995

This experiment was carried out to broadcating effect of chitosan solution levels on dry matter production in Ladino clover(Trifolium repens). Chitosan solution was applie at 6 levels, 0%, 0.01%, 0.05%, 0.1%, 0.5% and 1.0%, respectively. The results obtained were summarized as follows ; 1. The dry matter yields of chitosan treatment plot were about 30%~63% more than the dry matter yield of control plot in all cuts. 2. Total dry matter yield was produced by 659.7kg/10a at levels of 0.05%, it was significantly higher than other chitosan solution levels. 3. The predictable highest yield and economic yield of Ladino clover could be obtained at 0.05% and 0.01% solution levels, respectively. 4. The relative dry matter yields for total dry matter yield were 66.7%, 18.3% and 15.0% at 1st, 2nd and 3rd cut, respectively. Chitosan application at early spring was affected to increasing the dry matter yield of 1st cut.

• 한국식품영양과학회지
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• 제26권1호
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• pp.87-91
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• 1997

키토산을 생산하기 위하여 Cunninggamella blak-esleeana IFO 4443을 자외선조사하여 생성능이 우수한 Cunningmella blakesleeana IFO 4443-10의 변이주를 분리하였다. 본 변이주인 C. blakesleeana IFO 4443-10을 3$0^{\circ}C$에서 pH 4.5에서 3일간 발효하여 mycelia 발효액을 알칼리로 처리하여 chitosan을 얻었다. 이때 chitosan의 수율은 1012mg/l로 높았으며 탈아세틸화는 84.6% 이였다.

• 한국가정과학회:학술대회논문집
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• 한국가정과학회 1997년도 가정과학회 총회 및 학술대회
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• pp.33-37
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• 1997

Cotton fabric was treated with chitosan solution by pad-dry(-cure) method to impart antimicrobial properties. Four chitosans of different degree of deacetylation(DAC: 65~95％) with similar molecular weight(ca. 50, 000) and one chitosan oligomer(MW 1, 800, DAC 86％) were used. Antimicribial activity against Staphylococcus aureus was evaluated by the Shake Flask Method. Treated fabrics were laundered up to 20 times according to AATCC Test Method 60-1986 and antimicrobial activity of laundered fabrics was evaluated. The antimicrobial activity was increased with the concentration and DAC of chitosan used. And the cured samples showed better durability to laundering than not-cured samples. Crosslinker and binder decreased the antimicrobial activity of fabrics treated with chitosan oligomer and were not effective to improve the durability to washing.

• 한국의류산업학회지
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• 제7권4호
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• pp.445-451
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• 2005

Up to the present, fabric finishing methods have been mainly employed for the application of chitosan. However, the coated chitosan acid salt is prone to be detached from the fabric during the laundering process. In order to improve the wash fastness, chitosan treated fabrics are being subjected to thermal curing. In this study, chemical crosslinking reaction by epichlorohydrin was introduced to improve the problem of the thermal curing. An improvement of the wash fastness is expected since the coated chitosan component on the fabric become insoluble by the introduction of the crosslinking. The demerit of the single chitosan treatment (inferior handle due to the excessive stiffness, etc.) was supplemented by the application of chitosan-polyurethane mixture solution. The mixture ratios were adjusted to 1 : 0, 1 : 0.25, 1 : 0.5, 1 : 1, and 1 : 2 for the chitosan/PU mixture. Physical and mechanical properties of the finished fabric specimens were measured using the Kawabata Evaluation System(KES), and hand values were calculated accordingly based on the translational formulas.

• 한국식물병리학회지
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• 제14권3호
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• pp.278-285
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• 1998

Effects of chitosan on growth of tomato plant, and suppression of Fusaruim wilt caused by Fusarium oxysporum f. sp. lycopersici and late blight casued by Phytophthora infestans, were examined. Both late blight and fusarium wilt were suppressed by spray and irrigation of chitosan, respectively. Inhibition of mycelial growth was not greatly affected by molecular size of chitosan but, concentration dependent effects was observed. Ninty percent of P. infestans and 80% of F. oxysporum f. sp. lycopersici of mycelial growth was inhibited by 1,000 ppm of chitosan (MW 30,000~50,000) when amended in plate media. Induction of defense-related gene expression in plant by chitosan treatments were observed when chitosan treated tobacco and tomato RNA samples were hybridized with several defense-related genes as probes. The results revealed that $\beta$-1,3-glucanase and chitinase genes were strongly induced, while pathogenesis-related protein-1, 3-hydroxy-3-methylglutaryl coenzyme A reductase, anionic peroxidase, phenylalanine ammonia lyase genes were weakly induced by chitosan treatment. These results suggest that chitosan have dual effects on these host-pathogen interactions. Possible roles of chitosan in suppression of tomato diseases by inhibition of mycelial growth and activation of plant defense responses are discussed.