• Journal of Applied Biological Chemistry
• /
• v.63 no.2
• /
• pp.147-152
• /
• 2020

Farrerol is a flavanone isolated from the traditional Chinese herb 'Man-shan-hong' (Rhododendron dauricum L.). Farrerol has been reported to have various bioactivities including anti-oxidant, anti-inflammation, and anti-fungal. However, anti-cancer effect of farrerol has not yet been reported in MCF-7 breast cancer cells. In the present study, we investigated the anti-cancer effect of farrerol on MCF-7 cells. Farrerol decreased viability and induced apoptosis of MCF-7 cells in a dose dependent manner. Ferrerol exhibited a significant anti-proliferation effect with a half-maximal inhibitory concentration (IC₅₀) values of 145.04±1.4 μM in MTT assay, when MCF-7 cells were treated with ferrerol for 48 h. Also, ferrerol induced apoptotic bodies of MCF-7 cells as evaluated by TUNEL assay and Annexin V/PI staining using FACS. By mechanism of action, ferrerol regulated the activation of p38 mitogen-activated protein kinase and altered the expression level of BAX, Bcl-2, and Poly ADP Ribose Polymerase in MCF-7 cells. In summary, our finding demonstrated that ferrerol has anti-cancer effect through regulating the activation and expression of apoptosis-related proteins in MCF-7 cells.

• Weed & Turfgrass Science
• /
• v.4 no.3
• /
• pp.209-218
• /
• 2015

This study was conducted to investigate the weed occurrence and distribution on the upland crop fields (garlic, barley, potato, maize, red pepper, soybean and Chinese cabbage) at 795 sites in Chungbuk province of Korea from Apr. to Oct. in 2014. From the result of this survey, 188 weed species in 42 families were identified and classified to 96 annuals, 40 biennials and 52 perennials. Based on the occurrence ratio, the most dominant weed species in Chungbuk province upland crop fields were higher in the order of Portulaca oleracea (8.14%), Digitaria ciliaris (6.72%), Echinochloa crus-galli (6.55%), Rorippa palustris (6.00%) and Chenopodium album (5.89%). The composition of major occurred weed families, Compositae, Poaceae, Polygonaceae and Cruciferae were 19, 10, 7 and 6%, respectively. In the winter upland crop fields, the dominant weeds were in the order of Capsella bursa-pastoris (12.36%), Alopecurus aequalis (11.05%) and Stellaria alsine (8.42%). In the summer upland crop fields, the dominant weeds were in the order of Portulaca oleracea (8.58%), Digitaria ciliaris (7.18%) and Rorippa palustris (6.28%). Fiftythree exotic weed species were identified. The results of this study could be useful information for estimation of future weed occurrence, weed population dynamics and establishment of weed control methods on the upland crop fields in Chungbuk province of Korea.

• Korean Journal of Medicinal Crop Science
• /
• v.11 no.2
• /
• pp.135-142
• /
• 2003

This study was conducted to clarify on germination rate enhancement effect and to develope the growing seedling method on Sorbus commixta Hedl. The results were follows; The optimum temperature of stratification and germination of mountain ash was $5^{\circ}C$ the seed germination rate in wet stratification with sand at $5^{\circ}C$ for 90 days was 22.6% and the highest germination of 78.9% was observed when seed was soaked in BA 200ppm for 20 minute after stratification for 90 days at $5^{\circ}C$. The highest germination of large chinese hawthorn was 11.1% when seed was soaked BA 50ppm for 20 minute after stratification 90 days at $5^{\circ}C$. Emergence ratio in box raising seedling and pot seedling showed high, 93 and 95%. Root length and dry root weight in pot seedling for 50 days showed higher than that of direct seedling and box raising seedling, Plant height and dry leaf weight in box raising seedling showed higher than that of pot seedling and direct seedling. The growth increment of seedling was high between 40 days and 50 days after seedling. The excellent seedling of mountain ash was produced in pot seedling for 50 days.

• Horticultural Science & Technology
• /
• v.35 no.1
• /
• pp.98-107
• /
• 2017

A DNA profile database was constructed to investigate the genetic relatedness of 72 germplasm samples of Pyrus and related cultivars using microsatellite markers. Three P. pyrifolia, four P. commus, and one P. betulifolia cultivars with different morphological traits were screened using 387 pairs of microsatellite primers. A core set of 11 primer pairs was selected to obtain 133 polymorphic amplified fragments meeting three criteria: high polymorphism information contents (PIC), high repeatability, and distinct allele patterns. The number of alleles per locus ranged between 4 and 22. Average PIC was 0.743 (range: 0.557 - 0.879). Cluster analysis using the unweighted pair - group method with arithmetical average (UPGMA) separated the 72 pear cultivars and germplasm samples into four major groups: Chinese, European pears, and a cluster of 55 Asian pears that could be reclassify into two subcluster, I - $1^{st}$ and II - $2^{nd}$, according to pedigree information. Almost all of the cultivars were discriminated by 11 microsatellite marker genotypes. The microsatellite DNA profile database may be utilized as tool to verify distinctness, uniformity, and stability between candidate cultivar, and to verify in the distinctness of existing cultivars.

• Korean Journal of Food Science and Technology
• /
• v.35 no.3
• /
• pp.461-469
• /
• 2003

Effects of fruit of Lycium chinense (Chinese matrimony vine) on enzyme activities, and microbial and physicochemical properties of kochjuang were investigated during 12 weeks of fermentation. Enzyme activities were higher during middle of fermentation, and protease activities increased as the ratio of L. chinense increased. Facultative anaerobic bacteria counts decreased in L. chinense-added kochujang, whereas viable cell count of yeasts was higher in 1% L. chinense-added kochujang. Consistency of kochujang decreased by addition of L. chinense. Hunter L- and a-values of L. chinense added kochujang were high, causing slight change in total color difference (E) as the ratio of L chinense increased. Moisture contents of kochujang increased during fermentation, whereas water activities decreased. As the ratio of L. chinense increased, water activities increased. Titrable acidities and oxidation-reduction potential increased by addition of L. chinense. Total sugar contents of kochujang decreased rapidly during fermentation, whereas reducing sugar contents increased up to $2{\sim}4$ weeks of fermentation. As the ratio of L. chinense increased, reducing sugar contents decreased. Ethanol contents of kochujang increased during fermentation, with higher values in 3% L. chinense-added kochujang. Amino and ammonia nitrogen contents of kochujang increased L. chinense content increased. After 12 weeks of fermentation, sensory results showed 3% L. chinense-added kochujang showed highest taste and overall acceptability, and color acceptability increased as L. chinense content increased.

• Food Science and Preservation
• /
• v.12 no.1
• /
• pp.80-85
• /
• 2005

The effect of 12 edible mushroom species on the antioxidant and cytotoxicity on cancer cells were studied Methanol extract of Lyophyllum ulmarium, Cordyceps militaris and Sarcodon aspratus showed $30\~60\%$ DPPH radical scavenging activity and $39\~53\%$ protective effects against the cytotoxicity of $B_2O_2$. Methanol extracts of Sarcodon aspratus, Lyophyllum ulmarium, Cordyceps militaris, Agaricus blazei and Ganoderma lucidum revealed high inhibitive activities in cytotoxicity on human leukemia tells such as promyelocytic leukemia cell (HL60) and histiocytic lymphoma cell (U937). Highest toxicity was observed against HL60 cells in Sarcodon aspratus methanol extract showing $70.5\%$ inhibition at 1mg/mL whereas Cordyceps militaris methanol extract showed $81.5\%$ inhibition against U937 cells. Most water extracts of edible mushrooms exhibited the lowest effect against HL60 and U937 cells compared to methanol extract. These extract did not show cytotoxic effects against human lymphocyte. Results revealed 5 kinds of edible murshroom (Cordyceps militaris, Agaricus blaxei, Lyophyllum ulmarium, Ganoderma lucidum and Sarcodon aspratus) have strong antioxidative and in vitro anticancer efforts.

• Journal of Astronomy and Space Sciences
• /
• v.34 no.4
• /
• pp.271-280
• /
• 2017

This study presents the application of satellite laser ranging (SLR) to orbit determination (OD) of high-Earth-orbit (HEO) satellites. Two HEO satellites are considered: the Quasi-Zenith Satellite-1 (QZS-1), a Japanese elliptical-inclinedgeosynchronous-orbit (EIGSO) satellite, and the Compass-G1, a Chinese geostationary-orbit (GEO) satellite. One week of normal point (NP) data were collected for each satellite to perform the OD based on the batch least-square process. Five SLR tracking stations successfully obtained 374 NPs for QZS-1 in eight days, whereas only two ground tracking stations could track Compass-G1, yielding 68 NPs in ten days. Two types of station bias estimation and a station data weighting strategy were utilized for the OD of QZS-1. The post-fit root-mean-square (RMS) residuals of the two week-long arcs were 11.98 cm and 10.77 cm when estimating the biases once in an arc (MBIAS). These residuals were decreased significantly to 2.40 cm and 3.60 cm by estimating the biases every pass (PBIAS). Then, the resultant OD precision was evaluated by the orbit overlap method, yielding three-dimensional errors of 55.013 m with MBIAS and 1.962 m with PBIAS for the overlap period of six days. For the OD of Compass-G1, no station weighting strategy was applied, and only MBIAS was utilized due to the lack of NPs. The post-fit RMS residuals of OD were 8.81 cm and 12.00 cm with 49 NPs and 47 NPs, respectively, and the corresponding threedimensional orbit overlap error for four days was 160.564 m. These results indicate that the amount of SLR tracking data is critical for obtaining precise OD of HEO satellites using SLR because additional parameters, such as station bias, are available for estimation with sufficient tracking data. Furthermore, the stand-alone SLR-based orbit solution is consistently attainable for HEO satellites if a target satellite is continuously trackable for a specific period.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.29 no.3
• /
• pp.295-303
• /
• 2002

This study was performed to establish the cephalometric standards in Downs analysis and to compare them with other races. The lateral cephalograms of 88 Korean children (47 boys, 41 girls) with normal occlusion and acceptable profile between 7 and 11 years of age were taken and Downs analysis was done. Results were as follows: 1. Norms of Korean boys, girls and both sexes at 7, 9, 11 years of age by Downs analysis were established. 2. There were no statistically significant differences between boys and girls about ten measurements in each group, but significant differences in the occlusal plane angle, lower 1 to occlusal plane angle, interincisal angle, upper 1 to APog plane among 3 groups(p<0.05)(p<0.01). 3. Facial angle, AB plane angle, Y-axis, lower 1 to occlusal plane angle and lower 1 to mandibular plane angle showed increasing tendency whereas angle of convexity, mandibular plane angle, occlusal plane angle and interincisal angle did decreasing tendency according to aging. 4. In comparison among races, measurements in Korean children were generally larger than those of Caucasian and Greek except for small interincisal angle, but Korean were similar to Japanese, Chinese, Israeli, Negro about all measurements.

• Korean journal of applied entomology
• /
• v.44 no.3 s.140
• /
• pp.213-217
• /
• 2005

Adult development and fecundity of the green peach aphid, Myzus persicae $S\H{u}lzer$, were studied at $15{\sim}32.5^{\circ}C$ with $60{\sim}70%$ RH under 16L:8D and the results were put together to build a life table. The longevity shortened as temperatures rose at and below $25^{\circ}C$ whereas it remained relatively constant at $27.5^{\circ}C$. Total fecundity was not significantly different at all temperatures except at $30^{\circ}C$. Daily fecundity gradually increased from $15\;to\;22.5^{\circ}C$. It was 5.1 at $25^{\circ}C$ and as temperatures either went down or up, it decreased to 2.8 at both $15^{\circ}C\;and\;30^{\circ}C$. Fecundity model built from total fecundity at various temperatures suggests that $18.3^{\circ}C$ was the optimum temperature for the maximum progeny of 51. Net reproduction rate RO was highest of 36.5 at $27.5^{\circ}C$. The intrinsic rate of increase per day $r_m$ and the finite rate of increase per day $\lambda$ were highest of 0.4 and 1.6, respectively and the doubling time Dt was shortest of 1.6. Constructed life table suggests that the optimum temperature for adult development for the green peach aphid was $27.5^{\circ}C$ at which the mean generation time was 8.1.

• Korean Journal of Food Science and Technology
• /
• v.35 no.2
• /
• pp.173-181
• /
• 2003

Physicochemical changes in corn starch caused by irradiation were investigated, and irradiated samples were identified. Viscosity, TBA value, Hunter color, and total viable count were measured after irradiation of corn starch. Corn starches from Korea and China were irradiated at 0, 2.5, 5, 7.5, 10, and 15 kGy using a $Co^{60}$ irradiator and stored for 9 months at $0^{\circ}C$ and $20^{\circ}C$. Viscosity and specific parameter values decreased in all samples with increasing irradiation dosage at 50 rpm, showing a dose-dependent relationship $(above\;R^2=0.80)$ between non-irradiated and irradiated samples during storage. These results suggest that detection of irradiated corn starches is possible using viscometric method during storage. Total viable count, TBA value, and Hunter color were determined as supplemental indices for measuring viscosities of samples. Total viable count and TBA values showed dose-dependent relationship $(2.5{\sim}15 kGy)$. Differences in viscosity and total viable count, and TBA values among non-irradiated samples showed little changes with the lapse of post-irradiation time, but were still distinguishable for more than 6 months at $0^{\circ}C$ and $20^{\circ}C$ for corn starches from korea and China.