• Title/Summary/Keyword: Chinese galls

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Analysis genetic diversity of Plasmodiophora brassicae using RFLP and RAPD(oral)

  • Heo, Seung-Hwan;Jang, Chang-Soon;Lee, Hyoun-Kyoung;Lee, Woo-Chung;Jang, Se-Jeong;Kim, Hong-Gi
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.112.1-112
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    • 2003
  • Genetic diversity of Plasmodiophora brassicae from major chinese cabbage cultivating areas in Korea was analyzed by using PCR-RFLP and RAPD. Single spores of P brassicae isolated from galls of club root made induce lesion on chinese cabbage successfully. The PCR-RFLP and RAPD by primers PbITS, URP 3, 6 and OPA 7 revealed that single spore isolates showed various DNA polymorphisms among them unrelated geographic origins. These results indicate that P. brassicae population in Korea showed genetic difference among them. This study could be facilitate to identify genetic characteristics ofP. brassicae based on DNA polymorphisms between single spore isolates and to get basic information which can be used to advanced resistance breeding against club root of chinese cabbage.

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Occurrence and Vertical Distribution of Meloidogyne incognita in Chinese Yam (Dioscorea batatas) (마 재배지의 뿌리혹선충의 발생 실태 및 수직 분포)

  • Kwon, Giyoon;Kang, Heonil;Seo, Jongmin;Yun, Eulsoo;Park, Namsook;Choi, Insoo
    • Research in Plant Disease
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    • v.25 no.3
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    • pp.124-130
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    • 2019
  • The study was conducted to investigate the infestation and distribution of plant-parasitic nematodes on Chinese yam (Dioscorea batatas) in Andong, Korea. Root-knot nematodes, Meloidogyne incognita was the most prevalent nematode species which is found from 43 yam fields (81.1%) with high population densities (average of 450 juveniles/$300cm^3$). Other nematodes, root-lesion nematode (Pratylenchus spp.) and pin nematode (Paratylenchus spp.) were less problematic. Density of juveniles (J2) of M. incognita was $10\;J2/300cm^3$ by August, then increased to 274 in September and 624 in October. The highest J2 density was found at the soil depth between 40 and 50 cm ($1,840\;J2/300cm^3$). M. incognita was able to infest yam tuber down to a depth of 70 cm and developed galls outside and brown spots inside. The highest number of females were found at 40-50 cm (79 females/10 cm piece) tuber from the top.

Some Environmental Factors Affecting Germination and Survival of Resting Spores of Plasmodioprora brassicae (배추무사마귀병균 휴면포자의 발아 및 생존에 미치는 몇가지 환경요인)

  • Kim, Choong-Hoe;Cho, Won-Dae;Kim, Hong-Mo
    • The Korean Journal of Pesticide Science
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    • v.4 no.4
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    • pp.66-71
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    • 2000
  • Effect of temperature on resting spore germination of Plasmodioprora brassicae was indirectly estimated based on examining temporal change of number of inactive resting spores. Resting spore germination was the highest at $28^{\circ}C$ reaching 55.6% and 82.5%, 24hr and 132hr after treatment, respectively. Optimum pH for resting spore germination was pH6, following pH7 and pH8, and the germination was inhibited at pH 4, and pH9. termination of resting spores was stimulated by root extracts of radish, Chinese cabbage and kidney bean, but inhibited by that of lettuce. Number of inactive resting spores was increased as temperature increases and time prolongs after temperature treatment. However, degree of inactivation of resting spores after 1hr at $40{\sim}65^{\circ}C$ was similar with $40{\sim}60%$, but rapidly increased to 91.5% at $70^{\circ}C$. When root galls were submerged in water, density of inactive resting spores was increased rapidly and reached 60.3% 9 days after treatment. Flooding of infested soil resulted in 30% reduction of survived resting spores 5 months later. Among the two registered fungicides, fluazinam was better for inactivation of resting spores than flusulfamide, but both fungicides were inferior to phosphoric acid.

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Gall formation on different age, habitat, and parasite position in Rhus javanica L. (붉나무의 수령, 서식지 및 기생위치에 따른 오배자 형성)

  • Jo, Hyeong-Chan;Han, Kwang-Soo;An, Eun-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.4
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    • pp.304-311
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    • 2000
  • This study was conducted to investigate the ratio and number of the Rhus javanica gall formed by chinese sumac aphid, Scheleechtendalia chinensis, in several different conditions in which R. javanica lives. The density of 11 to 15-year-old R. javanica was more higher and the number of galls was also higher than 1 to 5- or over 16-year-old one at these sites. The formed gall according to four directions such as the East, West, South and North was founded the highest at the East and followed South, North, and West order. The formative rate of R. javanica gall did not differ in the mixture forest, and in the habitat having a heap of a little stone and mosses near a valley was better than in the site of road perimeter. Through an index of the gall diameter and the number of chinese sumac aphid, we evaluated the gall processing and development formed by the aphid. As the density of aphids increase, the size of the gall was also more plumped. The part of phyllotaxy of R. javanica gall in leaf and pinnate of was increased in the middle and upper part of terminal leaflet and leaflet was more occupied than wing. R. javanica which did not form a gall in natural environmental, formed gall by a artificial inoculation of S. chinensis. Some gall disappeared during a gall development from various environmental conditions.

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