• Fisheries and Aquatic Sciences
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• v.8 no.3
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• pp.151-160
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• 2005

Genomic DNA samples isolated from Fenneropenaeus chinensis (fleshy prawn; FP) and Palaemon gravieri (Chinese ditch prawn; CDP) collected in the West Sea, off the Korean Peninsula, at Buan, were PCR-amplified repeatedly. The sizes of the DNA fragments generated by seven different primers varied from 50 bp to 1,600 bp. We identified 358 fragments for the FP species and 301 fragments for the CDP species. There were 18 polymorphic fragments (5.03$\%$) for the FP species and 12 (3.99$\%$) for the CDP species. In total, 66 common fragments (average of 9.4 fragments per primer) were observed for the FP species and 44 fragments (average of 6.3 fragments per primer) were observed for the CDP species. The numbers of specific fragments seen for the FP species and CDP species were 38 and 47, respectively. The complexity of the banding patterns varied dramatically between the primers and the two species. In the FP species, a specific fragment of approximately 1,200 bp generated by primer OPB-04 exhibited inter-individual-specific characteristics that were indicative of DNA polymorphisms. Moreover, in the CDP species, a major fragment of approximately 550 bp generated by primer OPB-20 was found to be specific for the CDP. The average bandsharing value between the two prawn species was 0.421$\pm$0.006, and ranged from 0.230 to 0.611. The dendrogram obtained using the data from the seven primers indicated seven genetic clusters: cluster 1, FLESHY 01, 02, 03, and 04; cluster 2, FLESHY 05, 06, and 07; cluster 3, FLESHY 08, 09, 10, and 11; cluster 4, DITCH 13, 14, 16, and 18; cluster 5, DITCH 12, 15, and 17; cluster 6, DITCH 19, 20, and 21; and cluster 7, DITCH 22. The genetic distance between the two prawn species ranged from 0.071 to 0.642. Thus, RAPD-PCR analysis revealed a significant genetic distance between the two prawn species. Using various arbitrary primers, RAPD-PCR may be applied to identify specific/polymorphic markers that are particular to a species and geographic population, and to define genetic diversity, polymorphisms, and similarities among shrimp species.

• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.90-91
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• 2005

• Animal Systematics, Evolution and Diversity
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• v.25 no.3
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• pp.237-242
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• 2009

The first zoeas of Chinese ditch prawn Palaemon gravieri (Yu, 1930) and carpenter shrimp Palaemon serrifer (Stimpson, 1860) are described and illustrated in detail based on laboratory-hatched material. They are distinguished from those of Palaemon ortmanni (Rathbun, 1902) and Palaemon pacificus (Stimpson, 1860), previously known larvae from Korean waters by the length of the rostrum and endopod of antenna, the shape of the third abdominal somite, and ornamentation of the fifth abdominal somite. Larval characters of the genus Palaemon are revised. A. provisional key to the known first zoeas of Palaemon from Korean waters is given.

• Journal of Food Hygiene and Safety
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• v.38 no.6
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• pp.496-507
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• 2023

This study investigated species identification and labeling compliance of 48 shrimp products sold in the Korean online markets. Species identification was conducted using the standard DNA barcoding method, using the cytochrome c oxidase subunit I gene. The obtained sequences were compared with those deposited in the NCBI GenBank and BOLD Systems databases. Additionally, phylogenetic analysis was performed to further verify the identified shrimp species. Consequently, 16 shrimp species were identified, including Penaeus vannamei, Pandalus borealis, Palaemon gravieri, Leptochela gracilis, Penaeus monodon, Pleoticus muelleri, Metapenaeopsis dalei, Euphausia pacifica, Lebbeus groenlandicus, Trachypenaeus curvirostris, Argis lar, Metanephrops thomsoni, Metapenaeopsis barbata, Alpheus japonicus, Penaeus chinensis, and Mierspenaeopsis hardwickii. The most prevalent species was Penaeus vannamei, found in 45.8% of the analyzed products. A significant mislabeling rate of 72.9% was found; however, upon excluding generic names such as shrimp, the mislabeling rate reduced to 10.4%. The mislabeling rate was higher in highly-processed products (89.3%) compared with that in minimally-processed products (50%). No correlation was found between the country of origin and mislabeling rate. The results of this study provide crucial data for future monitoring of shrimp products and improving the labeling of shrimp species in Korea.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.4
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• pp.452-468
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• 2012

Marine organisms were collected by the stow nets and the stow net on boat fisheries from April 2010 to November 2011 in the Han River Estuary and categorized as 126 species, 61 families, and 5 taxa. The species were consisted with 34 in Crustacea, 5 in Cephalopoda, 79 in Pisces, unidentified jellyfishes in Cnidaria, and Finless porpoise in Mammals. The major species were composed of fish and crustaceans in the Han River Estuary. The dominant species in Crustacea were Chinese ditch prawn (Palaemon gravieri), blue crab (Portunus trituberculatus), Ridgetail prawn (Exopalaemon carinicauda), and mantis crab (Oratosquilla oratoria), and those in Pisces were Korean anchovy (Coilia nasus), and Japanese grenadier anchovy (Coilia mystus). The length structures of the six dominant species have more than one mode in the Han River Estuary. It reflects that the species inhibit during a part of and/or whole lifetime in the Han River Estuary where they utilize as spawning and/or nursery grounds. Freshwater fishes were collected from station D where is the closest location to the Han River stream, and their appearances were well matched with the large amount of freshwater discharge due to flood periods. Principal components analysis (PCA) was carried out with species compositions and showed temporal and spatial differences by the variations of species.