• 제목/요약/키워드: Chinese Medical Journal

검색결과 1,616건 처리시간 0.03초

Impact of IL-2 and IL-2R SNPs on Proliferation and Tumor-killing Activity of Lymphokine-Activated Killer Cells from Healthy Chinese Blood Donors

  • Li, Yan;Meng, Fan-Dong;Tian, Xin;Sui, Cheng-Guang;Liu, Yun-Peng;Jiang, You-Hong
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권18호
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    • pp.7965-7970
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    • 2014
  • One of the goals of tumor immunotherapy is to generate immune cells with potent anti-tumor activity through in vitro techniques using peripheral blood collected from patients. However, cancer patients generally have poor immunological function. Thus using patient T cells, which have reduced in vitro proliferative capabilities and less tumor cell killing activity to generate lymphokine-activated killer (LAK) cells, fails to achieve optimal clinical efficacy. Interleukin-2 (IL-2) is a potent activating cytokine for both T cells and natural killer cells. Thus, this study aimed to identify optimal donors for allogeneic LAK cell immunotherapy based on single nucleotide polymorphisms (SNP) in the IL-2 and IL-2R genes. IL-2 and IL-2R SNPs were analyzed using HRM-PCR. LAK cells were derived from peripheral blood mononuclear cells by culturing with IL-2. The frequency and tumor-killing activity of LAK cells in each group were analyzed by flow cytometry and tumor cell killing assays, respectively. Regarding polymorphisms at IL-2-330 (rs2069762) T/G, LAK cells from GG donors had significantly greater proliferation, tumor-killing activity, and IFN-${\gamma}$ production than LAK cells from TT donors (P<0.05). Regarding polymorphisms at IL-2R rs2104286 A/G, LAK cell proliferation and tumor cell killing were significantly greater in LAK cells from AA donors than GG donors (P<0.05). These data suggest that either IL-2-330(rs2069762)T/G GG donors or IL-2R rs2104286 A/G AA donors are excellent candidates for allogeneic LAK cell immunotherapy.

In vitro Study of Nucleostemin as a Potential Therapeutic Target in Human Breast Carcinoma SKBR-3 Cells

  • Guo, Yu;Liao, Ya-Ping;Zhang, Ding;Xu, Li-Sha;Li, Na;Guan, Wei-Jun;Liu, Chang-Qing
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권5호
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    • pp.2291-2295
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    • 2014
  • Although nucleolar protein nucleostemin (NS) is essential for cell proliferation and early embryogenesis and expression has been observed in some types of human cancer and stem cells, the molecular mechanisms involved in mediation of cell proliferation and cell cycling remains largely elusive. The aim of the present study was to evaluate NS as a potential target for gene therapy of human breast carcinoma by investigating NS gene expression and its effects on SKBR-3 cell proliferation and apoptosis. NS mRNA and protein were both found to be highly expressed in all detected cancer cell lines. The apoptotic rate of the pcDNA3.1-NS-Silencer group ($12.1-15.4{\pm}3.8%$) was significantly higher than those of pcDNA3.1-NS ($7.2-12.0{\pm}1.7%$) and non-transfection groups ($4.1-6.5{\pm}1.8%$, P<0.01). MTT assays showed the knockdown of NS expression reduced the proliferation rate of SKBR-3 cells significantly. Matrigel invasion and wound healing assays indicated that the number of invading cells was significantly decreased in the pcDNA3.1-NS-siRNA group (P<0.01), but there were no significant difference between non-transfected and over-expression groups (P>0.05). Moreover, RNAi-mediated NS down-regulation induced SKBR-3 cell G1 phase arrest, inhibited cell proliferation, and promoted p53 pathway-mediated cell apoptosis in SKBR-3 cells. NS might thus be an important regulator in the G2/M check point of cell cycle, blocking SKBR-3 cell progression through the G1/S phase. On the whole, these results suggest NS might be a tumor suppressor and important therapeutic target in human cancers.

Comparative Effectiveness of Risk-adapted Surveillance vs Retroperitoneal Lymph Node Dissection in Clinical Stage I Nonseminomatous Germ Cell Testicular Cancer: A Retrospective Follow-up Study of 81 Patients

  • Fan, Gang;Zhang, Lin;Yi, Lu;Jiang, Zhi-Qiang;Ke, Yang;Wang, Xiao-Shan;Xiong, Ying-Ying;Han, Wei-Qin;Zhou, Xiao;Liu, Chun;Yu, Xie
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권8호
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    • pp.3267-3272
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    • 2015
  • Purpose: To retrospective assess the potential predictors for relapse and create an effective clinical mode for surveillance after orchidectomy in clinical stage I non-seminomatous germ cell testicular tumors (CSI-NSGCTs). Materials and Methods: We analyzed data for CSI-NSGCTs patients with non-lymphatic vascular invasion, %ECa < 50% (percentage of embryonal carcinoma < 50%), and negative or declining tumor markers to their half-life following orchidectomy (defined as low-risk patients); these patients were recruited from four Chinese centers between January 1999 and October 2013. Patients were divided into active surveillance group and retroperitoneal lymph node dissection (RPLND) group according to different therapeutic methods after radical orchidectomy was performed. The disease-free survival rates (DFSR) and overall survival rates (OSR) of the two groups were compared by Kaplan-Meier analysis. Results: A total of 121 patients with CSI-NSGCT were collected from four centers, and 81 low-risk patients, including 54 with active surveillance and 27 with RPLND, were enrolled at last. The median follow-up duration was 66.2 (range 6-164) months in the RPLND group and 65.9 (range 8-179) months in the surveillance group. OSR was 100% in active surveillance and RPLND groups, and DFSR was 89.8% and 87.0%, respectively. No significant difference was observed between these two groups ($X_2=0.108$, P=0.743). No significant difference was observed between the patients with a low percentage of embryonal carcinoma (<50%) and those without embryonal carcinoma (87.0% and 91.9%, $X_2=0.154$, P=0.645). No treatment-related complications were observed in the active surveillance group whereas minor and major complications were observed in 13.0% and 26.1% of the RPLND group, respectively. Conclusions: Active surveillance resulted in similar DFSR and OSR compared with RPLND in our trial. Patients with low-risk CSI-NSGCTs could benefit from risk-adapted surveillance after these patients were subjected to radical orchidectomy.

Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells

  • Zhang, Ruiping;Li, Jing;Li, Jianding;Xie, Jun
    • Molecules and Cells
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    • 제37권9호
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    • pp.650-655
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    • 2014
  • Mesenchymal stem cells (MSCs) can differentiate into neural cells to treat nervous system diseases. Magnetic resonance is an ideal means for cell tracking through labeling cells with superparamagnetic iron oxide (SPIO). However, no studies have described the neural differentiation ability of SPIO-labeled MSCs, which is the foundation for cell therapy and cell tracking in vivo. Our results showed that bone marrow-derived mesenchymal stem cells (BM-MSCs) labeled in vitro with SPIO can be induced into neural-like cells without affecting the viability and labeling efficiency. The cellular uptake of SPIO was maintained after labeled BM-MSCs differentiated into neural-like cells, which were the basis for transplanted cells that can be dynamically and non-invasively tracked in vivo by MRI. Moreover, the SPIO-labeled induced neural-like cells showed neural cell morphology and expressed related markers such as NSE, MAP-2. Furthermore, whole-cell patch clamp recording demonstrated that these neural-like cells exhibited electrophysiological properties of neurons. More importantly, there was no significant difference in the cellular viability and $[Ca^{2+}]_i$ between the induced labeled and unlabeled neural-like cells. In this study, we show for the first time that SPIO-labeled MSCs retained their differentiation capacity and could differentiate into neural-like cells with high cell viability and a good cellular state in vitro.

해외교육 치과의사의 국내유입에 따른 치과 의료의 질 보장 및 치과의사의 적정수급 - 일본과 중국을 중심으로 - (Quality assurance of dental care and appropriate supply of dentists in view of the increasing inflow of dental graduates from abroad)

  • 신제원;김윤진;김경년;김각균;이재일
    • 대한치과의사협회지
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    • 제55권1호
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    • pp.7-20
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    • 2017
  • The purpose of this study is to analyze the data on the medical personnel education system and license scheme and provide the basic material for an enhanced qualification system. In China, dental education reform has been underway with a view to providing an inclusive basic medical service package to the whole country by 2020. It is also estimated that the number of Korean dental medicine students in China would be fewer. And most of them desire to get a job in China after acquiring the Chinese license, suggesting little prospect of a massive inflow into Korea in the near future. In Japan, students are required to complete a clinical training program for over 1 year after receiving licenses to become an independent practitioner. But they can apply for the Korean preliminary examination without this post-graduation process. For this reason, the quality issue in license effectiveness review has been seriously discussed. It is deemed desirable to limit the Korean accreditation to Japanese graduates from the dental schools certified by the Japanese dental accreditation body.

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Tamil traditional medicinal system - siddha: an indigenous health practice in the international perspectives

  • Karunamoorthi, Kaliyaperumal;Jegajeevanram, Kaliyaperumal;Xavier, Jerome;Vijayalakshmi, Jayaraman;Melita, Luke
    • 셀메드
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    • 제2권2호
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    • pp.12.1-12.11
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    • 2012
  • Traditional Medicinal System (TMS) is one of the centuries-old practices and long-serving companions to the human kind to fight against disease and to lead a healthy life. Every indigenous people have been using their unique approaches of TMS practice where among, the Chinese, Indian and African TMSs are world-wide renowned. India has a unique Indian System of Medicines (ISM) consisting of Ayurveda, Siddha, Unani, Naturopathy and Homoeopathy. Siddhars are the saints as well as the eminent scholars, who have attained Ashta-mahasiddhi [Tamil: (Ashta-Eight; Mahasiddhi-Power)] or enlightment. They have postulated, practiced, immensely contributed and have established the concept of the Tamil medicinal system called Siddha System of Medicine (SSM). From ancient time, SSM has flourished and has been widely practiced in the southern part of India particularly in Tamil Nadu. The induction of the modern medicinal system has immensely influenced the existence of SSM and has made the SSM principles and practices undervalued/extinct. However, at present, still a considerable group of people are using the SSM as a basic health-care modality. In this context, the present scrutiny deals with the TMS history, its significance with a special reference to SSM history, Siddhars, the basic concept of SSM, its diagnostic procedures, materia medica and treatment. Conclusively, Siddha is one of the most ancient indigenous health practices despite its several thorny challenges and issues, which needs to be flagged effectively and to be preserved and revitalized in the international arena in the near future.

필담(筆談)을 통한 조일(朝日) 의원(醫員) 간 소통의 방식 - 1763년 계미사행(癸未使行)의 필담을 중심으로 - (A study on the communication between the medical staff of Chosun and Japan to conversation by writing)

  • 김형태
    • 동양고전연구
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    • 제41호
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    • pp.187-209
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    • 2010
  • 본 논문은 1763년 계미사행(癸未使行) 중 의원필담집(醫員筆談集)인 키타야마 쇼우[北山彰]의 "계단앵명(?壇?鳴)"과 야마다 세이친[山田正珍]의 "상한필어(桑韓筆語)"와 이 빈케이[(井敏卿]의 "송암필어(松菴筆語)"를 연구 대상으로 삼아 18세기에 의원을 포함한 조일(朝日) 지식인들의 소통 방식 및 대립과 갈등, 교류와 협력의 상황을 확인하는 데 그 목적이 있다. 그들은 미지(未知)와의 조우를 통해 나누고, 시문(詩文)을 창화(唱和)했으며, 해부학 등 당시 서양의학에 눈뜬 일본의 상황을 가늠해보기도 했다. 또한 각 나라의 풍습을 접하고 비교하는 기회를 가졌으며, 아울러 한글에 대한 일본인의 증폭된 관심을 확인할 수 있다. 대립과 갈등은 주로 두 나라의 역사 인식과 학술적 차원의 관점 차이에서 비롯되었는데, 특히 당시 일본의 고학(古學)적 학풍이 여기에 상당한 영향을 끼쳤다고 할 수 있다. 따라서 금고문(今古文) 논쟁이나 두 나라의 음악 비교, 일본 신공황후(神功皇后)의 삼한(三韓) 정벌설 및 오규 소라이[荻生?徠]와 다자이 준[太宰純]의 학문에 대한 논쟁 등으로 상호 날선 공방이 오고갔다. 다만 간과하지 말아야 할 것은 이 중에는 한문에 의한 필담이란 제한 때문에 빚어진 상호 오해도 존재한다는 점이다. 교류와 협력은 전문 지식과 기술을 지닌 의원들을 중심으로 인삼 제법(製法)이나 질병의 치료 방법을 확인하는 절차로 구체화되었다. 또한 일본에서의 서적 구입 방법 문의 및 실제 치료 등을 통해 양국 우호의 분위기를 연장시켜 나갔다고 볼 수 있다. 결국 이들 사이에는 간혹 오해와 불신도 있었지만, 양국의 인사들은 한문이란 동아시아 공통문자를 통해 소통했고, 심도 있는 정보와 의견을 교환했다.

당대 향문화 연구 (A Study on the Culture of Incense in the Period of T'ang)

  • 전혜숙;이애련
    • 한국의상디자인학회지
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    • 제7권3호
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    • pp.113-127
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    • 2005
  • From the ancient times, incense was used for various usages including a means of beauty expression with flavor, a medicine for disease treatment and a device for religious event or ritual. The period of T'ang was the times when cultural and material exchanges with foreign countries were very actively made under the political openness of the Chinese nation. Here the exchanges were made mainly through inland trade, called Silk Road(絲綢之路) and marine trade routes, Incense Road(香料之路). This indicates that incense was one of the main items actively traded at that time. In addition, literatures of the T'ang period show that in the Chinese nation, a wide range of classes from the imperial family to the public used incense for many different purposes. This suggests that the culture of incense was deeply prevailed and very socially significant in T'ang. This study investigated social factors that promoted the incense culture of T'ang and the applications and types of incense widely used in the period of T'ang. First, influential religions and the openness of sex culture were main social factors that made incense culture flourish in the period of T'ang. Above all, two main religions of the Chinese nation, Buddhism and Taoism became secularized under political protection by the imperial family. As Buddhism was popularized, the Buddhist ritual of incense burning made a contribution to making public incense culture. Providing its doctrines of eternal youth and eternal life, Taoism necessarily used incense to form a Taoistic climate. The flourishment of the foresaid religion in T'ang added more fuel to that of incense culture in the Chinese nation. The openness of sex culture brought about the Inauguration of the empress, improvement in female position and free relationships between man and woman. It was accelerated by sexology as a method of eternal youth provided by Taoism. The opened culture also developed the culture of kibang where female entertainers called kinyeo consumed lots of incense for decoration and sexual desire stimulation. These open climates of T'ang society made a great contribution to making incense culture, especially for decoration, prevailed throughout the Chinese nation. Second, types of incense prevailed and widely used in the period of T'ang included olive incense, germander(廣藿香), olibnum(乳香), myrrh Resinoid(沒藥), jia Xiang(甲香), clove(丁香) and Shen xian(沈香), all of which were imported from foreign nations and had various applications. Specifically, olive incense, germander(廣藿香), olibnum(乳香) and myrrh Resinoid(沒藥) were used for religious purposes while, jia Xiang(甲香), clove(丁香) and Shen xian(沈香) for the purposes of religion and decoration. In conclusion, a number of social factors including political, religious and medical purposes and the openness of sex culture set fundamentals on which the culture of incense was extensively developed and established as a social trend in T'ang. In the Chinese nation, incense culture was not just an option for taste, but a part of life style social members needed to know. People of T'ang not only enjoyed incense mainly for purposes of religion, pleasure and make-up, but also had the wisdom to know various effects of incense, curiosity about such new things and the will to imitate and pursue alien culture, resultantly flourishing incense culture. Thus the culture of incense represented many social aspects of T'ang.

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Chemical Components of Paecilomyces tenuipes (Peck) Samson

  • Hong, In-Pyo;Nam, Sung-Hee;Sung, Gyoo-Byung;Chung, In-Mo;Hur, Hyeon;Lee, Min-Woong;Kim, Mi-Kyung;Guo, Shun-Xing
    • Mycobiology
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    • 제35권4호
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    • pp.215-218
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    • 2007
  • The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Paecilomyces tenuipes that formed on the living silkworm (Bombyx mon) host were used in this examination. This study was carried out to investigate the proximate composition, soluble sugar, amino acid and fatty acid profiles, and contents of the bioactive ingredient including adenosine and D-mannitol in the fruiting-bodies. The moisture content was 57.56%. Soluble sugars found were glycerol, glucose, mannitol and sucrose, and the contents exceeded $24\;mgg^{-1}dry$ weight. Total free amino acid content was $17.09\;mgg^{-1}dry$ weight. Arginine, glycine, proline and tyrosine were main amino acids. The content of oleic acid in fatty acids was high. Adenosine was more abundant in fruiting bodies than corpus.

Effects of Saccharomyces cerevisiae and phytase co-fermentation of wheat bran on growth, antioxidation, immunity and intestinal morphology in broilers

  • Chuang, Wen-Yang;Lin, Li-Jen;Hsieh, Yun-Chen;Chang, Shen-Chang;Lee, Tzu-Tai
    • Animal Bioscience
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    • 제34권7호
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    • pp.1157-1168
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    • 2021
  • Objective: The aim of this study was to investigate the effects of different amounts of wheat bran (WB) inclusion and postbiotics form by Saccharomyces cerevisiae and phytase co-fermented wheat bran (FWB) on the growth performance and health status of broilers. Methods: Study randomly allocated a total of 300 male broilers to a control and 4 treatment groups (5% WB, 5% FWB, 10% WB, and 10% FWB inclusion, respectively) with each pen having 20 broilers and 3 pens per treatment. Results: The WB does not contain enzymes, but there are 152.8, 549.2, 289.5, and 147.1 U/g dry matter xylanase, protease, cellulase and β-glucanase in FWB, respectively. Furthermore, FWB can decrease nitric oxide release of lipopolysaccharide stimulated chicken peripheral blood mononuclear cells by about two times. Results show that 10% FWB inclusion had significantly the highest weight gain (WG) at 1 to 21 d; 5% FWB had the lowest feed conversion rate at 22 to 35 d; 10% WB and 10% FWB inclusion have the highest villus height and Lactobacillus spp. number in caecum; and both 5% and 10% FWB can increase ash content in femurs. Compared to control group, all treatments increase mucin 2, and tight junction (TJ), such as occludin, claudin-1, zonula occludens-1, and mRNA expression in ileum by at least 5 folds. In chicken peripheral blood mononuclear cells, nicotinamide adenine dinucleotide phosphate-oxidase-1 mRNA expression decreases from 2 to 5 times, and glutamate-cysteine ligase catalytic subunit mRNA expression also increases in all treatment groups compared to control group. The mRNA expression of pro-inflammatory cytokines, including interleukin-6 (IL-6), nuclear factor-κB, and IL-1β, decreases in 5% and 10% FWB groups compared to control group. Conclusion: To summarize, both WB and FWB inclusion in broilers diets increase TJ mRNA expression and anti-oxidation and anti-inflammation, but up to 10% FWB groups have better WG in different stages of broiler development.