• Korean Journal of Pharmacognosy
• /
• v.7 no.3
• /
• pp.225-232
• /
• 1976

To compare the effects of red Ginseng and white Ginseng on the growth of experimental animals, both Ginseng were given to Broiler male chicken. Red and white Ginseng were administered to Broiler chicken in doses of 0.25, 0.5, 1, 2 and 4g per kg weight of chicken. The increase in weight were 282g in red Ginseng group and 162g in white Ginseng group compared with control group. The increase of the feeding amounts per kg weight were 2.4kg for red Ginseng group, 2.67kg for white Ginseng group and 2.87kg for non-treated group. The growth of each organ in the red Ginseng group showed favorable increase trend than white Ginseng group as a whole and, particularly, considerable significance were observed in liver and brain. These results suggest that red Ginseng has better effect on the growth of chicken and organ than white Ginseng does.

• Korean Journal of Poultry Science
• /
• v.34 no.2
• /
• pp.85-90
• /
• 2007

The chicken liver has been involved in various biological functions including detoxification, glycogen storage and plasma protein synthesis. The aim of this study was to investigate differentially expressed genes in chicken liver in four different growing stages. Using 10 arbitrary Annealing Control Primers (ACPs), five differentially expressed genes have been identified. Based on the Basic Local Alignment Search Tool (BLAST) search results, three of them were matched with previously known genes, and the other two were matched with unknown EST sequence and a hypothetical protein, respectively. In order to confirm the expression results, quantitative real-time PCR was also performed. The high similarities between the expression data using arbitrary ACPs and quantitative real-time PCR indicate that the identified genes are the real differentially expressed genes in different growing stages. The genes identified in this study can be used as valuable biomarkers in chicken with further investigation of the functions.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.3
• /
• pp.336-342
• /
• 2009

Avian embryos at high altitude are independent of maternal protection against hypoxia, which is contrary to mammals. It is well known that chronic hypoxic exposure at key points can significantly impact on avian development. Tibetan Chicken, a Chinese indigenous breed, living in Tibetan areas with an altitude of 2.2 to 4.1 thousand meters, has an adaptive mechanism to hypoxia. In the present study, fertilized eggs of Tibetan Chicken were incubated under 13% and 21% oxygen concentration. Two lowland chicken breeds, Shouguang Chicken, an indigenous chicken breed in Shandong Province of China, and Dwarf Recessive White Chicken, an imported breed in Beijing, were used as control groups. The embryo mass and some organs such as brain, heart, liver, stomach and eye weight in the three species were measured at Hamburger-Hamilton stage 39, 41, 43 and 45 under hypoxic and normal conditions. The results showed that in hypoxia Tibetan Chicken significantly differed from the two lowland chicken breeds in embryo mass at Hamburger-Hamilton stage 41, 43 and 45 (p<0.01). In particular, Dwarf Recessive White Chicken and Shouguang Chicken showed retarded growth in hypoxic incubation (p<0.01), whereas Tibetan Chicken showed no significant difference between hypoxic and normal conditions (p>0.05). In addition, heart and the other organs showed different susceptibility to hypoxia at the studied stages. In conclusion, chronic hypoxia induced a change in the embryo development of the three different species and Tibetan Chicken showed adaptation to hypoxia. Of note, the embryo developmental physiology of Tibetan Chicken in response to hypoxia will shed light on the process of physiological acclimation or evolutionary adaptation as well as the study of clinical disease.

• Korean Journal of Veterinary Research
• /
• v.34 no.3
• /
• pp.593-599
• /
• 1994

This study was carried out to investigate the necrotic enteritis of chicken in Korea. Epidemiological, pathological and microbiological examination were done on 12 naturally occurred cases of necrotic enteritis of chicken. And the susceptibility of isolated bacteria to antimicrobial agents was also examined. The results obtained were as follows; 1. The Clostridium perfringens infection, necrotic enteritis of chicken, occurred in the chickens mainly raised in floor pens. Necrotic enteritis of chicken was occurred coincidently with coccidiosis and Gumboro disease frequently. And several cases were recurred at 2-3 weeks after recovery. 2. Clinical signs of the infected chickens were depression, decreased appetite, reluctance to move, diarrhea, ruffled feathers and acute death within several hours. 3. The characteristic biochemical properties of isolates were 2-band hemolysis, no motility, positive reaction of reverse CAMP test and the formation of LV precipitate in egg yolk medium. 4. Gross lesions of the infected chickens were distention of intestine with gas, thickened mucosa and formation of thick pseudomembrane in intestine. Livers were friable with yellowish brown color and, in some case, showed demarcated necrotic foci. 5. Histopathological findings of the infected chickens were severe necrosis of the intestinal mucosa and attachment of numerous large bacilli to the mucosal surface of necrotic villi. In liver, necrosis of liver tissue and numerous large bacilli in the necrotic foci were also observed. 6. In susceptibility test to antimicrobial agents, 12 isolates of Clostridium perfringens were highly sensitive to ampicillin, baytril, cephalothin and penicillin.

• Asian-Australasian Journal of Animal Sciences
• /
• v.23 no.7
• /
• pp.855-862
• /
• 2010

Among the known interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful pathways. The Mx protein has direct antiviral activity and inhibits a wide range of viruses by blocking an early stage of the viral replication cycle. Cloning, characterization, and expression of Mx in vivo and in vitro have been conducted. The chicken Mx gene spans 21 kb and is made up of 14 exons and 13 introns, of which the promoter region was analyzed. The real-time PCR results showed that Mx expression was increased in chicken embryo fibroblasts (CEF) after 12- and 24-h induction with polyI: C. Induction of Mx expression by poly I: C in vivo revealed tissue-specific patterns among the chicken tissues tested. A trace expression of Mx was detected in healthy chicken liver tissues from adult chickens without inducement; the expression levels in the liver, heart, and gizzard were higher than in the muscle and kidney. This is the first report to demonstrate the expression of a glutathione-S-transferase-tagged-Mx fusion protein of 75 KDa, as well as the biological activity tested by SDS-PAGE and western blotting.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.2
• /
• pp.155-161
• /
• 2009

Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.

• Journal of the East Asian Society of Dietary Life
• /
• v.19 no.3
• /
• pp.363-368
• /
• 2009

In this experiment, three different diets were produced to investigate the effects of Jeju native chicken meat, from chickens that were fed a tangerine by-product, on physiological activities in rats. The first diet did not contain any chicken (TS), the second diet contained 10% chicken that had not been fed the tangerine by-product (T0), and the third diet contained 10% that had been fed the by-products (T1). These diets were provided to 11-week-old male rats for four weeks. Weight gain, feed intake, feed efficiency, liver, kidney, and epididymis fat weights were not significantly different among the TS, T0, and T1 groups. Total lipid, triglycerides, and cholesterol levels in the liver were significantly lower in T0 and T1 than in TS (p<0.05). And total lipid, phospholipid, triglycerides, total cholesterol, HDL-cholesterol, DL+VLDL-cholesterol, HDL-cholesterol/total cholesterol, and atherogenic index levels in the blood serum were similar between T0 and T1, which did not present any significant differences. The feed containing the tangerine by-product did not cause any statistically significant differences in serum protein, glucose, or hemoglobin. Finally, T0 and T1 showed similar trends in terms of $\gamma$-GTP, ALT, AST, and ALP activities, which again did not present any statistically significant differences.

• Korean Journal of Veterinary Research
• /
• v.31 no.3
• /
• pp.271-277
• /
• 1991

To investigate the epidemiological trait of listeriosis, Listeria spp were isolated from poultry meat, products and environmental specimens in chicken slaughterhouse. Also determined were isolation rates by the different enrichment procedures, the biochemical properties of isolates. In a total of 307 samples including poultry meat, liver, feathers, feces, chiller water, scalding water overflow and slaughterhouse floor, Listeria spp were isolated predominantly from scalding water overflow (90.0%), body skin before washing (66.7%), liver (20.0%) and feathers(15.0%) However, few Listeria spp were isolated from body skin after washing and feces. The higher isolation rates were obtained in the secondary enrichment procedure (7.2%) than in the primary enrichment (3.9%); after stored the secondary enrichment cultures for 2 weeks at $4^{\circ}C$, Listeria spp were present in 9.8%. The majority of the isolated Listeria spp were identical to those of the standards strains in biochemical and cultural properties. Overall, Listeria spp were present in 13.4% of the specimens tested, and were in order of prevalence of L innocua(11.1%), L monocytogenes(3.3%), L grayi(0.7%) and L murrayi(0.3%).

• Asian-Australasian Journal of Animal Sciences
• /
• v.6 no.3
• /
• pp.339-342
• /
• 1993

This influence of dietary medium chain triglyceride on liver protein synthesis in chicks was investigated using a large dose injection of $L-[4-^3H]$ phenylalanine. Dietary medium chain triglyceride increased liver weight and liver fat content of chicks compared to the long chain triglyceride group. Fractional synthesis rate of liver protein was increased by dietary medium chain triglyceride, which was accounted for not by elevating protein synthesized per unit RNA but by enhancing RNA: protein ratio.

• Korean Journal of Veterinary Research
• /
• v.34 no.3
• /
• pp.457-463
• /
• 1994

Insulin-like growth factor-I(IGF-I) plays an important role in the regulation of mammalian and poultry growth. IGF-I has many actions in different tissues, which include metabolic, mitogenic, and differentiative actions. IGF-I induces insulin-like effects - such as increased cell glucose uptake and glycogen sysnthesis, however several physiological actions of IGF-I may not have been identified yet. In order to investigate the effect on growth in broiler chicken treated with exogenous insulin-like growth factor-I, 30 chickens were injected $50{\mu}g$ reconbinant human IGF- I (rhIGF- I ) per kg body weight as experimental group and 30 ckickens saline subcutanously as control, 3 times according to ages from 2 to 35 days. We established radioimmunoassay method by which we can measure chicken IGF- I (cIGF- I ) as in rhIGF- I assay. The results obtained were as follows; 1) The dilution curve showed in parallelism between rhIGF- I and cIGF- I in the Sep-pak $C_{18}$ cartridge plasma extracts. 2) The body weight of broiler chicken were significantly increased at 31 days($1,176.50{\pm}99.79g$) and 35 days($1,252.84{\pm}125.21g$) of age in treatment groups, compared with control group($1,011.88{\pm}40.22g,\;1,111.32{\pm}153.67g$). The liver and kidney weights on 35 days$(35.24{\pm}5.18g,\;11.05{\pm}1.47g)$ were significantly higher in rhIGF- I treated group than control group($30.95{\pm}4.04g,\;10.01{\pm}1.60g$) 3) The plasma concentration of IGF- l and total protein in rhIGF- I treated group were $58.17{\pm}1.69ng/ml$, $3.75{\pm}0.62g/dl$ respectively compared with control group $45.70{\pm}1.64ng/ml$, $2.32{\pm}0.53g/dl$. The results suggest that exogenous rhIGF- I increased total body weight, liver and kidney weights in broiler chicken, and it may increase IGF- I and total protein concentration in serum.