• 한국산학기술학회논문지
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• 제22권3호
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• pp.446-452
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• 2021

우리나라 생활수준의 향상과 더불어 식품소비의 양적인 요구가 충족되면서, 세분화된 식품의 기호 성향을 충족시킬 수 있는 닭고기 소비가 증가하고 있다. 2003년 3월 축산물 품질평가원에서 고시(농림부 고시 제2003-14호)한 닭 도체 품질판정세부기준은 닭 도체 부위별 이물질 부착, 피·멍의 크기 및 중량에 따라 품질 등급을 기준을 제시하였다. 그러나 현실적으로 검사관 개개인의 주관적인 평가 기준으로 적용된 고시로 수천 마리의 닭 도체 등급판정을 유지하기가 어려운 문제점을 가지고 있다. 본 논문에서는 닭 도체 품질 세부기준에 따라 닭 도체 부위 분할하기 위해 비접촉/비파괴방식인 컴퓨터 시각 기술 알고리즘을 제안한다. 제안된 알고리즘은 실시간으로 빠르게 움직이는 닭 도체를 부위 분할하기 위하여 조명 외란에 강인하도록 보정하는 과정과 닭 도체와 배경을 구분하기 위한 EM(Expectation maximization), Erosion 및 Labeling 알고리즘, 그리고 닭 도체의 기하학적 형태를 분석하여 부위별 특징점을 찾고 점들의 위치를 계산하여 부위를 분할 할 수 있는 알고리즘을 사용하였다. 총 78마리의 닭 도체 샘플에 대하여 제안한 영상처리 알고리즘을 적용한 결과 닭 도체 부위 분할 알고리즘이 효과적임을 알 수 있었다.

• 대한수의학회지
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• 제51권3호
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• pp.193-201
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• 2011

An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times ($10^{1.3}$) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea.

• 한국가금학회지
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• 제45권4호
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• pp.285-290
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• 2018

본 연구는 국제규격인 KS A ISO 2859-1 수월한 검사 방법을 계육에 처음으로 적용하여, 현행 닭고기의 등급 기준샘플링 방법을 대처할 수 있는지의 가능성을 알아보고자 수행하였다. 불량품 선별 능력을 확인한 결과, 현 등급 기준은 0.61, KS A ISO 2859-1 보통 검사 방법은 0.70으로 후자의 방법이 보다 정확히 선별할 수 있었다. 샘플링 방법에 따른 비용을 비교한 결과, 하루 50로트를 검사하는데 40,530원이 절감되었다. 또한 현 등급 기준과 KS A ISO 2859-1 수월한 검사는 12호, 9호, 8호 닭도체에서 모두 $1^+$등급을 충족시키기 위한 불량품 수가 불합격 한계를 넘어서지 않았다. 샘플링 방법에 따른 신선도의 유의성 검증은 전수검사와 현 등급 기준, KS A ISO 2859-1 수월한 검사에서 12호, 9호, 8호 닭도체의 모두에서 유의적인 차이가 나타나지 않았다. 이를 통해 샘플량이 적은 KS A ISO 2859-1 수월한 검사를 사용하는 것이 검사비용 절감의 효과를 가지면서 현재의 방법과 동일한 수준의 품질평가를 할 수 있는 것으로 판단되었다.

• 한국동물위생학회지
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• 제36권3호
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• pp.193-201
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• 2013

Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqMan$^{(R)}$ quantitative real-time polymerase chain reaction (PCR) assay was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient ($R^2$) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not contaminated with chicken residuals. Only twelve pork products of one of the manufacturers were in accordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consumers' trust.

• 대한수의학회지
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• 제52권4호
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• pp.285-288
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• 2012

Salmonella I 4,[5],12:i:- was a monophasic variant of Salmonella (S.) Typhimurium and notorious for re-emerging candidate which would replace S. Typhimurium DT104 for antibiotic resistance. Recently, isolation rate was increased on human and industrial animals but there was no case in domestic animals but human in Korea. This was first isolation case from domestic animals in Korea. The five isolates from feces of duck (n = 3), chicken (n = 1), and wild bird (n = 1) showed antibiotic resistance against cephems and aminoglycosides. These means that the spread of emerging bacterial pathogens to domestic animals and the need of systemic management for Salmonella I 4,[5],12:i:-.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.403-412
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• 1993

This paper presents the progress of the development of a nondestructive technique for the classification of normal, septicemic , and cadaver poultry carcasses by the Instrumentation and Sensing Laboratory at Beltsville, Maryland, U.S.A. The Sensing technique is based on the diffuse reflectance spectroscopy of poultry carcasses.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.755-764
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• 1996

A multispectral imaging system with selected wavelength optical filter was demonstrated feasible for food safety inspection. Intensified multispectral images of carcasses were obtained with visible/near-infrared optical filters(542-847 nm wavelengths) and analyzed. The analysis of textural features based on co-occurrence matrices was conducted to determine the feasibility of a multispectral image analyses for discriminating unwholesome poultry carcasses from wholesome carcasses. The mean angular second moment of the wholesome carcasses scanned at 542 nm wavelength was lower than that of septicemic (P$\leq$0.0005) and cadaver(P$\leq$0.0005) carcasses. On the other hand, for the carcasses scanned at 700nm wavelength , the feature values of septicemic and cadaver carcasses were significantly (P$\leq$0.0005) different from wholesome carcasses. The discriminant functions for classifying poultry carcasses into three classes (wholesome, septicemic , cadaver) were developed using linear and quadr tic covariance matrix analysis method. The accuracy of the quadratic discriminant models, expressed in rates of correct classification, were over 90% for the classification of wholesome, septicemic, and cadaver carcasses when textural features from the spectral images scanned at the wavelength of 542 and 700nm were utilized.

• Journal of Biosystems Engineering
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• 제40권2호
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• pp.145-152
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• 2015

Purpose: The inability to adequately judge the efficacy of cleaning and sanitation procedures in deli departments is a recognized food safety concern. In a prior study, our research group demonstrated that visual inspection of cleaned produce processing surfaces could be enhanced through the use of a portable fluorescence imaging device that detected residual produce residues. Methods: To explore the feasibility of using fluorescence imaging to similarly detect residual deli residues, spectra of American, Cheddar, Provolone, and Swiss cheeses and of processed chicken, ham, roast beef, and turkey were acquired using a laboratory hyperspectral imaging system. Circular punches of these commodities were placed onto stainless steel and high density polyethylene coupons for imaging. The coupon materials were selected to represent common surfaces found in deli departments. Results: Analysis of hyperspectral fluorescence images showed that cheeses exhibited peaks in the blue-green region and at around 675 nm. Meats exhibited peaks in the blue-green region with one of four ham and one of four chicken brands exhibiting peaks at around 675 nm, presumably due to use of plant-derived additives. When commodities were intermittently imaged over two weeks, locations of spectral peaks were preserved while intensity of peaks at shorter wavelengths increased with time. Conclusion: These results demonstrate that fluorescence imaging techniques have the potential to enhance surface hygiene inspection in deli departments and, given the immediate availability of imaging results, to help optimize routine cleaning procedures.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1380-1387
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• 2015

This study aims to determine the polymorphism and mRNA expression pattern of the heart-type fatty acid-binding protein (H-FABP) gene and their association with intramuscular fat (IMF) content in the breast and leg muscles of Baicheng oil chicken (BOC). A total of 720 chickens, including 240 black Baicheng oil chicken (BBOC), 240 silky Baicheng oil chicken (SBOC), and 240 white Baicheng oil chicken (WBOC) were raised. Three genotypes of H-FABP gene second extron following AA, AB, and BB were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) strategy. The G939A site created AA genotype and G956A site created BB genotype. The content of IMF in AA genotype in breast muscle of BBOC was significantly higher than that of AB (p = 0.0176) and the genotype in leg muscle of WBOC was significantly higher than that of AB (p = 0.0145). The G939A site could be taken as genetic marker for higher IMF content selecting for breast muscle of BBOC and leg muscle of WBOC. The relative mRNA expression of H-FABP was measured by real-time PCR at 30, 60, 90, and 120 d. The IMF content significantly increased with age in both muscles. The mRNA expression level of H-FABP significantly decreased with age in both muscles of the three types of chickens. Moreover, a significant negative correlation between H-FABP abundance and IMF content in the leg muscles of WBOC (p = 0.035) was observed. The mRNA expression of H-FABP negatively correlated with the IMF content in both breast and leg muscles of BOC sat slaughter time.

• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.7-12
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• 2009

Lmbr1 is regarded as a key gene that controls the digital model formation in early developmental stages of the chicken. However, there are few reports of lmbr1 expression levels and tendencies in 4-toe and 5-toe chicken species. Therefore, the objective of this study was to compare the lmbr1 expression in White Leghorn (4-toe) and Chinese Silky (5-toe). Firstly, total RNA was extracted from 14 different embryonic development stages (HH3 to HH31) in White Leghorn and Chinese Silky. Secondly, dramatic gene expression changes of lmbr1 were monitored by RT-PCR, which indicated a general up-down-up tendency with subtle differences between these two species. Moreover, Q-PCR reactions were performed to quantitate the expression level of lmbr1 in the 14 selected developmental stages. These data demonstrated a first lmbr1 expression peak of 18.68 and 15.32, a lmbr1 expression trough of 6.61 and 1.80, and a second lmbr1 expression peak of 22.33 and 12.48 in White Leghorn and Chinese Silky, respectively. Finally, embryonic in situ hybridization analysis identified that lmbr1 expressed in the ectoderm in HH21, HH23 and HH24 developmental stages in both species.