• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.446-452
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• 2021

As a higher standard for food consumption is required, the consumption of chicken meat that can satisfy the subdivided food preferences is increasing. In March 2003, the quality criteria for chicken carcasses notified by the Livestock Quality Assessment Service suggested quality grades according to fecal contamination and the size and weight of blood and bruises. On the other hand, it is too difficult for human inspection to qualify mass products, which is key to maintaining consistency for grading thousands of chicken carcasses. This paper proposed the computer vision algorithm as a non-destructive inspection, which can identify chicken carcass parts according to the detailed standards. To inspect the chicken carcasses conveyed at high speed, the image calibration was involved in providing robustness to the side effect of external lighting interference. The separation between chicken and background was achieved by a series of image processing, such as binarization based on Expectation Maximization, Erosion, and Labeling. In terms of shape analysis of chicken carcasses, the features are presented to reveal geometric information. After applying the algorithm to 78 chicken carcass samples, the algorithm was effective in segmenting chicken carcass against a background and analyzing its geometric features.

• Korean Journal of Veterinary Research
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• v.51 no.3
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• pp.193-201
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• 2011

An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times ($10^{1.3}$) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea.

• Korean Journal of Poultry Science
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• v.45 no.4
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• pp.285-290
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• 2018

This study was aimed to evaluate the possibility of application to the KS A ISO 2859-1 Easy test, an international standards sampling inspection, to replace a sampling method of the current grading standard of chicken meats for the first time. As a result of screening capability to check for defective products, the current sampling was 0.61 and the KS A ISO 2859-1 Normal test was 0.70, suggesting that the latter inspection method was more accurate. If the KS A ISO 2859-1 conversion principle is applied, 40,530 won per day is saved for a 50-lot inspection. The current sampling and the KS A ISO 2859-1 Easy test did not exceed the reject limit for the number of defective products to satisfy the grade $1^+$ on chicken carcass size numbers 12, 9 and 8. Freshness was not significantly different between the current sampling and the KS A ISO 2859-1 Easy test. It is expected that using the KS A ISO 2859-1 Easy test with a smaller sample volume will have the same level of quality evaluation as the current sampling method while reducing costs.

• Korean Journal of Veterinary Service
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• v.36 no.3
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• pp.193-201
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• 2013

Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqMan$^{(R)}$ quantitative real-time polymerase chain reaction (PCR) assay was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient ($R^2$) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not contaminated with chicken residuals. Only twelve pork products of one of the manufacturers were in accordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consumers' trust.

• Korean Journal of Veterinary Research
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• v.52 no.4
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• pp.285-288
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• 2012

Salmonella I 4,[5],12:i:- was a monophasic variant of Salmonella (S.) Typhimurium and notorious for re-emerging candidate which would replace S. Typhimurium DT104 for antibiotic resistance. Recently, isolation rate was increased on human and industrial animals but there was no case in domestic animals but human in Korea. This was first isolation case from domestic animals in Korea. The five isolates from feces of duck (n = 3), chicken (n = 1), and wild bird (n = 1) showed antibiotic resistance against cephems and aminoglycosides. These means that the spread of emerging bacterial pathogens to domestic animals and the need of systemic management for Salmonella I 4,[5],12:i:-.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.403-412
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• 1993

This paper presents the progress of the development of a nondestructive technique for the classification of normal, septicemic , and cadaver poultry carcasses by the Instrumentation and Sensing Laboratory at Beltsville, Maryland, U.S.A. The Sensing technique is based on the diffuse reflectance spectroscopy of poultry carcasses.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.755-764
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• 1996

A multispectral imaging system with selected wavelength optical filter was demonstrated feasible for food safety inspection. Intensified multispectral images of carcasses were obtained with visible/near-infrared optical filters(542-847 nm wavelengths) and analyzed. The analysis of textural features based on co-occurrence matrices was conducted to determine the feasibility of a multispectral image analyses for discriminating unwholesome poultry carcasses from wholesome carcasses. The mean angular second moment of the wholesome carcasses scanned at 542 nm wavelength was lower than that of septicemic (P$\leq$0.0005) and cadaver(P$\leq$0.0005) carcasses. On the other hand, for the carcasses scanned at 700nm wavelength , the feature values of septicemic and cadaver carcasses were significantly (P$\leq$0.0005) different from wholesome carcasses. The discriminant functions for classifying poultry carcasses into three classes (wholesome, septicemic , cadaver) were developed using linear and quadr tic covariance matrix analysis method. The accuracy of the quadratic discriminant models, expressed in rates of correct classification, were over 90% for the classification of wholesome, septicemic, and cadaver carcasses when textural features from the spectral images scanned at the wavelength of 542 and 700nm were utilized.

• Journal of Biosystems Engineering
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• v.40 no.2
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• pp.145-152
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• 2015

Purpose: The inability to adequately judge the efficacy of cleaning and sanitation procedures in deli departments is a recognized food safety concern. In a prior study, our research group demonstrated that visual inspection of cleaned produce processing surfaces could be enhanced through the use of a portable fluorescence imaging device that detected residual produce residues. Methods: To explore the feasibility of using fluorescence imaging to similarly detect residual deli residues, spectra of American, Cheddar, Provolone, and Swiss cheeses and of processed chicken, ham, roast beef, and turkey were acquired using a laboratory hyperspectral imaging system. Circular punches of these commodities were placed onto stainless steel and high density polyethylene coupons for imaging. The coupon materials were selected to represent common surfaces found in deli departments. Results: Analysis of hyperspectral fluorescence images showed that cheeses exhibited peaks in the blue-green region and at around 675 nm. Meats exhibited peaks in the blue-green region with one of four ham and one of four chicken brands exhibiting peaks at around 675 nm, presumably due to use of plant-derived additives. When commodities were intermittently imaged over two weeks, locations of spectral peaks were preserved while intensity of peaks at shorter wavelengths increased with time. Conclusion: These results demonstrate that fluorescence imaging techniques have the potential to enhance surface hygiene inspection in deli departments and, given the immediate availability of imaging results, to help optimize routine cleaning procedures.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.10
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• pp.1380-1387
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• 2015

This study aims to determine the polymorphism and mRNA expression pattern of the heart-type fatty acid-binding protein (H-FABP) gene and their association with intramuscular fat (IMF) content in the breast and leg muscles of Baicheng oil chicken (BOC). A total of 720 chickens, including 240 black Baicheng oil chicken (BBOC), 240 silky Baicheng oil chicken (SBOC), and 240 white Baicheng oil chicken (WBOC) were raised. Three genotypes of H-FABP gene second extron following AA, AB, and BB were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) strategy. The G939A site created AA genotype and G956A site created BB genotype. The content of IMF in AA genotype in breast muscle of BBOC was significantly higher than that of AB (p = 0.0176) and the genotype in leg muscle of WBOC was significantly higher than that of AB (p = 0.0145). The G939A site could be taken as genetic marker for higher IMF content selecting for breast muscle of BBOC and leg muscle of WBOC. The relative mRNA expression of H-FABP was measured by real-time PCR at 30, 60, 90, and 120 d. The IMF content significantly increased with age in both muscles. The mRNA expression level of H-FABP significantly decreased with age in both muscles of the three types of chickens. Moreover, a significant negative correlation between H-FABP abundance and IMF content in the leg muscles of WBOC (p = 0.035) was observed. The mRNA expression of H-FABP negatively correlated with the IMF content in both breast and leg muscles of BOC sat slaughter time.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.1
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• pp.7-12
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• 2009

Lmbr1 is regarded as a key gene that controls the digital model formation in early developmental stages of the chicken. However, there are few reports of lmbr1 expression levels and tendencies in 4-toe and 5-toe chicken species. Therefore, the objective of this study was to compare the lmbr1 expression in White Leghorn (4-toe) and Chinese Silky (5-toe). Firstly, total RNA was extracted from 14 different embryonic development stages (HH3 to HH31) in White Leghorn and Chinese Silky. Secondly, dramatic gene expression changes of lmbr1 were monitored by RT-PCR, which indicated a general up-down-up tendency with subtle differences between these two species. Moreover, Q-PCR reactions were performed to quantitate the expression level of lmbr1 in the 14 selected developmental stages. These data demonstrated a first lmbr1 expression peak of 18.68 and 15.32, a lmbr1 expression trough of 6.61 and 1.80, and a second lmbr1 expression peak of 22.33 and 12.48 in White Leghorn and Chinese Silky, respectively. Finally, embryonic in situ hybridization analysis identified that lmbr1 expressed in the ectoderm in HH21, HH23 and HH24 developmental stages in both species.