• KSBB Journal
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• v.18 no.1
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• pp.51-54
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• 2003

The objective of this study was to optimize culture conditions and to produce hydrogen and organic compounds using microalga Chlamydomonas reinhardtii. First of all, C. reinhardtii UTEX 90 was chosen from the three kinds of strains in terms of their hydrogen and organic compound productivity. The optimum $\textrm{CO}_2$ concentration range of C. reinhardtii UTEX 90 was 1to 3%. We tested two medium, which are popular in this microalga culture; Brostol's medium and TAP medium (8). The cell growth in TAP medium was found to be higher than a Brostol's medium. Optimum culture with 3% of $\textrm{CO}_2$ in TAP medium produced the most hydrogen ($0.5\mu$ mol/ mg DCW), though Bristol's medium produced twice as much total organics.