• Korean Journal of Pharmacognosy
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• v.45 no.4
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• pp.300-314
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• 2014

An ultra-performance liquid chromatography-electrospray ionization-mass spectrometer (UPLC-ESI-MS) method was established for the simultaneous quantification of eighteen marker compounds in traditional Korean formula, Cheonwangbosimdan (CWBSD). In addition, we evaluated the antioxidant effects of CWBSD. Eighteen marker components were separated on a UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) and kept at $45^{\circ}C$ by gradient elution with 0.1% (v/v) formic acid in water and acetonitrile as mobile phase. The flow rate was 0.3 mL/min and the injection volume was $2.0{\mu}L$. The antioxidant activities of CWBSD were assessed by measuring free radical scavenging activities on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH). The calibration curves of all analytes showed good linearity (correlation coefficient ${\geq}0.9937$) within the test ranges. The limits of detection and quantification for the 18 marker compounds were 0.01-4.71 ng/mL and 0.03-14.13 ng/mL, respectively. The contents of the 18 compounds in CWBSD extract ranged from none to $1701.00{\mu}g/g$. The CWBSD showed the radical scavenging activity in a dose-dependent manner. The concentration required for 50% reduction ($RC_{50}$) against ABTS and DPPH radicals were $149.42{\mu}g/mL$ and $339.24{\mu}g/mL$.

• Journal of Oriental Neuropsychiatry
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• v.32 no.2
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• pp.129-140
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• 2021

Psychotherapy in Korean Medicine is characterized not only by management of mental issues, but also a holistic perspective of the mind and body, which includes physical treatment. In this case report, we describe the efficacy of Korean psychotherapy for couples with physical symptoms of heartache, emotional tension and marital relationships by addressing the challenges at the Mind and Body levels. For the physical treatment of the couple, the wife was treated with a Bunshimgi-Eum and the husband was administered a Cheonwangbosimdan, combined with a psychiatric interview based on Korean Medicine. It involves listening to the couple's story, YiJungBeongi therapy, understanding and learning about vases and defense mechanisms, self-understanding and understanding of the husband through self-understanding and expansion, and husband's understanding of the wife's position, self-interpretation and acceptance. Based on counseling, the couple's personal characteristics and expansion for self-growth, the progress and results of the couple's challenges and relationship improvement in a relatively short period of time are presented. In response, we hope that the evidence based on Korean Psychotherapy supporting the counseling for couples will continue to accumulate. We would like to report and share a few opinions.