• Bulletin of the Korean Chemical Society
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• v.25 no.12
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• pp.1845-1849
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• 2004

Apolipoprotein B-100 (Apo-B100) is a major protein component for low density lipoproteins (LDL). A number of mimetic peptides of Apo-B100 were screened from the phase-displayed random peptide library by utilizing monoclonal antibody (B9). Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF (pB1). From the BLAST search, the mimetic peptide pB1 had 40% homology with apo B-100. As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of 0.3-0.6 ${\AA}^2$ and the total RMSD was 0.5-1.5 ${\AA}. Moreover, pB1 structure included a weak $3_{10}$-helix from $Ile^7$,/TEX> to $Trp^{13}$.

• Bulletin of the Korean Chemical Society
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• v.20 no.11
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• pp.1335-1339
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• 1999

The function of 5S rRNA, a constituent of a large subunit of ribosome, is not clearly known yet. To identify RNA motifs interacting with 5S rRNA, and thereby to get an insight into the function of 5S rRNA in the ribosome, a SELEX (Systematic Evolution of Ligands by Exponential Enrichment) experiment was performed. RNA molecules binding to Escherichia coli 5S rRNA were selected from a 48-mer random sequence library through 12 rounds of selection, cloned, and sequenced. Two groups of the selected RNA molecules had the consensus sequences GCGG and GUGAAA, respectively, which are present in the segment, G688 through A696, of E. coli 16S rRNA. The gel mobility shift assay showed that 5S rRNA interacted with the 16S rRNA fragment containing the GCGG and GUGAAA sequences. The enzymatic protection experiment shows that the A29CCUGA34 and G51AAGUG56 sequences of 5S rRNA and the C680AGG683 and G688CGG691 sequences of the 16S rRNA fragment are involved in the interaction between the two RNA molecules. On the basis of this observation, we suggest that 5S rRNA and 16S rRNA play a role for the association of two ribosomal subunits.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.6 no.1
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• pp.133-160
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• 1984

The main purpose of the present study is to survey the major abstracting bulletins of national nature in Korea, to define such problem areas as lacunae, duplicates and limitation in coverage in the abstracting services currently available in Korea, and to make some suggestions for action for improving the abstracting services in the light of general principles and the tradition and situations unique to Korea. The major conclusions reached at this study are summarised as follows: (A) A new abstracting bulletin of general nature covering the whole field needs to be created in each of the following fields where no established abstracting service is available for the outcome of research and development activities in Korea. (1) Language (2) Religion (3) Art (4) Language (5) Literature (6) History (B) A new specialised abstracting bulletin needs to be created in each of the following fields of science where abstracting services limited in coverage are partially available. (a) Statistics (b) Sociology (c) political science (d) Public administration (e) Law (f) Folk lore (g) Military science (2) Pure sciences (a) Mathematics (b) Chemistry (c) Astronomy (d) Geology (e) Mineralogy (f) Life sciences (g) Botany (h) Zoology (3) Applied sciences (a) Agriculture (b) Architectural engineering (c) Mechanical engineering (d) Electrical engineering (e) Chemical engineering (f) Manufacturing industry (g) Domestic science (C) Publication of the abstracting bulletins suggested in (A) and (B) above may be ideally carried on by a qualified learned society established in the respective field. and should be financially supported by the public fund under the provisions of Art. 27 of the Research Promotion Act of 1979. (D) The current practice of adding the author's abstract and keywords to each of the records of the "Doctoral Theses in Humanities and Social Sciences" part of the" Catalogue of Doctoral and Master's Theses Submitted to the Universities in Korea" published by the National Assembly Library should be applied to all the other parts, i. e. to the parts of the "Master's Theses in Humanities and Social Sciences" and of the "Doctoral and Master's Theses in Natural Sciences': which will not only increase the Catalogue's use value but also discourage appearance of various theses abstracts of individual academic institutions such as the" Abstracts of the Doctoral and Master's Theses Submitted to Korea Advanced Institute of Science and Technology" which will in turn reduce inefficiency involved in the abstracting services at national level. (E) A general abstracting bulletin covering most part of the outcome of research and development activities in Korea other than that covered by the existing abstracts needs to be created to be temporarily. used till the abstracting journals suggested in this study will be fully available. A realistic way of having such a bulletin may be to expand the present coverage of "The Abstracts of the Reports of the Government-sponsored Projects" currently published by Korean Research Foundation.

• BMB Reports
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• v.43 no.12
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• pp.789-794
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• 2010

A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-${\beta}1$ and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.

• Proceedings of the Microbiological Society of Korea Conference
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• 2008.05a
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• pp.118-118
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• 2008

The phylogenetic diversity of microbial communities in calcareous mats from Spearfish Creek, a freshwater stream located in the Black Hills of South Dakota, was examined using PCR-based 16S rDNA sequence analysis. In this study, two types of calcareous mats were compared: mature mats formed on the natural substrate of rock surfaces and developing mats on an artificial substrate of glass slides. Among 63 unique isolates from a clone library of 16S rRNA genes from mature mat samples, there were 8 phyla of Bacteria represented. The predominant phylum was Proteobacteria (48%), with the $\beta$ subclass being the largest group. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes from slide samples collected at intervals for four months showed considerable diversity of the microbial community from the earliest stages of community development. Amplicons isolated from DGGE gels and sequenced indicated that community succession has occurred without increasing microbial diversity. However, light microscopic analysis revealed a significant increase in microbial cell density throughout the community development. Scanning electron microscopy of mat samples provides evidence that diatoms are also important members of calcareous mat communities.

• Archives of Pharmacal Research
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• v.27 no.4
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• pp.371-375
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• 2004

The silicon linker is the foremost traceless linker used in solid-phase reactions. Hydrogen fluo-ride (HF) or trifluoroacetic aicd (TFA) can remove the silicon linker with the silicon atom being replaced by a hydrogen atom. In this experiment, the linkers 1c and 2d, which are the most useful in solid-phase reactions, were synthesized, Linker 1c is composed of seven linearly linked carbons and linker 2d includes an oxygen atom in the linear carbon chain to increase the solvation capacity. The carboxylic acid component of linker 1c and 2d forms an amide or ester bond with resin. The synthesized linkers 1c and 2d could be utilized in constructing a chemical compound library that includes indole, benzodiazepine and phenothiazine (aromatic ring compounds).

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.35.2-35.2
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• 2013

I will present a new approach to constrain galaxy physical parameters from the combined interpretation of stellar and nebular emission in wide ranges of observations. This approach relies on a comprehensive library of synthetic spectra, assembled using state-of-the-art models of star formation and chemical enrichment histories, stellar population synthesis, nebular emission and attenuation by dust. We focus on the constraints set by 5-band photometry and low- and medium-resolution spectroscopy at optical rest wavelengths on a few physical parameters characterizing the stars and interstellar medium. Since these parameters cannot be known a priori for any galaxy sample, we assess the accuracy to which they can be retrieved by simulating 'pseudo-observations' using models with known parameters. We find that the combined analysis of stellar and nebular emission in low-resolution (50A FWHM) galaxy spectra provides valuable constraints on all physical parameters. The approach can be extended to the analysis of any type of observation and during this talk i will present some applications to observed galaxies up to redshift 1.5.

• BMB Reports
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• v.44 no.8
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• pp.517-522
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• 2011

Mitochondrial dynamics not only involves mitochondrial morphology but also mitochondrial biogenesis, mitochondrial distribution, and cell death. To identify specific regulators to mitochondria dynamics, we screened a chemical library and identified niclosamide as a potent inducer of mitochondria fission. Niclosamide promoted mitochondrial fragmentation but this was blocked by down-regulation of Drp1. Niclosamide treatment resulted in the disruption of mitochondria membrane potential and reduction of ATP levels. Moreover, niclosamide led to apoptotic cell death by caspase-3 activation. Interestingly, niclosamide also increased autophagic activity. Inhibition of autophagy suppressed niclosamide-induced cell death. Therefore, our findings suggest that niclosamide induces mitochondria fragmentation and may contribute to apoptotic and autophagic cell death.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1288-1288
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• 2001

X-ray powder diffraction (XRD) is utilized for determination of polymorphism in crystalline organic materials. Though convenient to use in a laboratory setting, XRD is not easily adapted to in situ monitoring of synthetic chemical production applications. Near-Infrared spectroscopy (NIR) can be adapted to in situ manufacturing schemes by use of a source/detector probe. Conversely, NIR is unable to conclusively define the existence of polymorphism in crystalline materials. By combining the two techniques, a novel simultaneous NIR/XRD instrument has been developed. During material's analysis, results from XRD allow for defining the polymorphic phase present, and NIR data are collected as a fingerprint for each of the observed polymorphs. These NIR fingerprints will allow for the development of a library, which can be referenced during the use of a NIR probe in manufacturing settings.

• 한국연소학회:학술대회논문집
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• 2007.05a
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• pp.118-125
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• 2007

The firtst-order conditional moment closure (CMC) model is applied to CH4/air swirl diffusion flame in a gas turbine model combustor. The flow and mixing fields are calculated by fast chemistry assumption with SLFM library and a beta function pdf for mixture fraction. RNG k-e model is used to consider the swirl flame in a confined wall. Reacting scalar fields are calculated by elliptic CMC formulation with chemical kinetic mechanism, GRI Mech 3.0. Validation is done against measurement data for mean flow and scalar fields in the model combustor [1]. Results show reasonable agreement with the mean mixture fraction and its variance, while temperature is overpredicted as the level of local extinction increases. The second-order CMC model is needed to consider local extinction with considerable conditional fluctuations near the nozzle.