• Title/Summary/Keyword: Chemical experiment

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Probing the Functional Motifs of Escherichia coli 5S rRNA in Relation to 16S rRNA Using a SELEX Experiment

  • 고재형;조봉래;안정근;이용훈;박인원
    • Bulletin of the Korean Chemical Society
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    • v.20 no.11
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    • pp.1335-1339
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    • 1999
  • The function of 5S rRNA, a constituent of a large subunit of ribosome, is not clearly known yet. To identify RNA motifs interacting with 5S rRNA, and thereby to get an insight into the function of 5S rRNA in the ribosome, a SELEX (Systematic Evolution of Ligands by Exponential Enrichment) experiment was performed. RNA molecules binding to Escherichia coli 5S rRNA were selected from a 48-mer random sequence library through 12 rounds of selection, cloned, and sequenced. Two groups of the selected RNA molecules had the consensus sequences GCGG and GUGAAA, respectively, which are present in the segment, G688 through A696, of E. coli 16S rRNA. The gel mobility shift assay showed that 5S rRNA interacted with the 16S rRNA fragment containing the GCGG and GUGAAA sequences. The enzymatic protection experiment shows that the A29CCUGA34 and G51AAGUG56 sequences of 5S rRNA and the C680AGG683 and G688CGG691 sequences of the 16S rRNA fragment are involved in the interaction between the two RNA molecules. On the basis of this observation, we suggest that 5S rRNA and 16S rRNA play a role for the association of two ribosomal subunits.

A Study on the Chemical Treatment Techniques of High Concentration Ammonia Nitrogen in Food Wastewater

  • Tae-Hwan JEONG;Su-Hye KIM;Woo-Taeg KWON
    • Journal of Wellbeing Management and Applied Psychology
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    • v.6 no.3
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    • pp.33-36
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    • 2023
  • Purpose: Since the food wastewater contains a high concentration of nitrogen, it is very important to find a way to efficiently remove it. Research design, data and methodology: A total of four experiments were conducted under different conditions to remove ammonia nitrogen present in the food wastewater. The experiment was designed by adding sodium hypochlorite to the raw food wastewater and varying conditions such as pH control, aeration/precipitation, and stirring. Results: The ammonia nitrogen removal rate in Experiment 1 was about 12% (sodium hypochlorite added), ammonia nitrogen increased about 4.7% in Experiment 2 (sodium hypochlorite added after aeration/precipitation in a bioreaction tank, stirring), and decreased about 52.5% (sodium hypochlorite added after controlling and stirring). Conclusions: When the concentration of sodium hypochlorite was high, ammonia nitrogen was best removed, and the pH was adjusted to 12, and sodium hypochlorite was added after stirring, and the removal was the second best. If the method of this study is further studied and developed, it can be basic data for ammonia nitrogen removal in the future.

Studies on the Change of Nonvolatile Organic acids and Higher Fatty acids during the curing of Flue-cured tobaccos (황색종 잎담배의 건조과정중 비휘발성 유기산과 지방산의 변화에 관한 연구)

  • Rhee, Moon-Soo;Lee, Un-Chul
    • Journal of the Korean Society of Tobacco Science
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    • v.4 no.2
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    • pp.75-80
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    • 1982
  • During the process of curing of the tobacco (Nicotiana tabacum L. ) leaves the concentration change of Nonvolatile organic acids and Higher fatty acids of the flue-cured tobacco leaves was observed. The contents of Nonvolatile organic acids in flue-cured tobacco leaves were as follows, malic> oxalic>malonic>citric>succinic acid. Higher fatty acids was linolenic>Palmitic>oleic >stearic$\geqq$linoleic acid, Citric acid content of last curing time was increased about 4~5 times in compare to before curing tobacco. It was concluded that significant changes of three variety flue-cured tobacco but a significant difference was observed in the composition of Daegu gold variety.

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A Study on Industrial Preparation Method of Ammonium Sulfate by Non-catalytic Oxidation (無觸媒酸化에 依한 黃酸암모늄의 工業的 製法에 關한 硏究)

  • Chung, Ki-Yong
    • Journal of the Korean Chemical Society
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    • v.10 no.2
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    • pp.76-90
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    • 1966
  • Ammonium sulfate synthesized by the air oxidation methods without catalyst using the reaction vessel which was fitted with fritted glass at the bottom of it and introducing, through the bottom, ammonia and air with constant flow rates to sulfurous acid solution of constant concentrations at the given temperatures. The experiment showed that the oxidation process was accelerated in accord with the increase of the air flow rates when the ammonia flow rate was constantly kept at ca. 100ml/min. in high temperatures. When the pH of the solution reached 9.0, the oxidation was nearly completed. It is assumed that in the process of reaction, $[O_{2}{\to}HSO_{3}^-]^{\neq}$ would be produced as an activated complex and the reaction was thought to be first order. The experiment indicated that the 0.5M sulfurous solution could be oxidized up to 98.54% at the flow rates of ammonia and air, 100ml/min., and 4l/min., respectively at $50^{\circ}C$.

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Impact of Current Density, Operating Time and pH of Textile Wastewater Treatment by Electrocoagulation Process

  • Hossain, Md. Milon;Mahmud, Md. Iqbal;Parvez, Md. Shohan;Cho, Haeng Muk
    • Environmental Engineering Research
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    • v.18 no.3
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    • pp.157-161
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    • 2013
  • Treatment of textile wastewater by the electrocoagulation (EC) process is being investigated by this experimental study. The objective of this experiment is to observe the efficiency of the EC process in removing chemical oxygen demand (COD) and turbidity. In this experiment an iron electrode is used in the EC process, and different working parameters such as pH, current density and operating time were studied in an attempt to achieve a higher removal capacity. The results show that the maximum COD removal occurred at neutral pH at operating time 30 min. COD and turbidity removal reaches at maximum, with optimum consumption of electrodes, between current density 85-95 $A/m^2$, and only trace amounts of metals were determined in the EC treated effluent.

Optimization of Cu CMP Process Parameter using DOE Method (DOE 방법을 이용한 Cu CMP 공정 변수의 최적화)

  • Choi, Min-Ho;Kim, Nam-Hoon;Kim, Sang-Yong;Chang, Eui-Goo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.11a
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    • pp.711-714
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    • 2004
  • Chemical mechanical polishing (CMP) has been widely accepted for the global planarization of multi-layer structures in semiconductor manufacturing. However, it still has various problems to the CMP equipment, in particular, among the CMP components, process variables are very important parameters in determining the removal rate and non-uniformity. Using a design of experiment (DOE) approach, this study was performed investigating the interaction between the various parameters such as turntable and head speed, down force and back pressure during CMP. Using statistical analysis techniques, a better understanding of the interaction behavior between the various parameters and the effect on removal rate, no-uniformity and ETC (edge to center) is achieved.

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Preliminary Molecular Dymanics Simulation Studies of H-Y Zeolite in a Non-Rigid Zeolite Framework

  • 최상구;이송희
    • Bulletin of the Korean Chemical Society
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    • v.20 no.4
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    • pp.445-450
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    • 1999
  • Molecular dynamics (MD) simulation of non-rigid H-Y zeolite framework are performed at 298.15 and 5.0 K. Usual bond stretching, bond angle bending, torsional rotational, and non-bonded Lennard-Jones and electrostatic interactions are considered as intraframework interaction potentials. Calculated atomic parameters are in good agreement with the experiment, which indicates the successful reproduction of the framework structure and its motion. Both calculated bond lengths and bond angles are also in good agreement with the experiment except generally for a little longer bond lengths and a little smaller T-O-H bond angles. The calculated overall site occupation of HI keeps the order O(2) > O(3) > O(4) > O(t) at 298.15 K, which is very different from the experimental prediction, O(l) > O(3) > O(2) at 5 K. Calculated IR spectra of the H-Y zeolite framework show that most of the main peaks of the O-H bonds are in the broad region 3700-5000 cm-1 and that the O-T stretching bands appeared in 0-2000 cm-1 and at 2700 cm-1

Oligomerization State of the Plasma Membrane Proteolipid Apoprotein Purified from the Bovine Kidney, Probed by the Fluorescence Polarization

  • Chae, Quae;Nam, Sang-Rye
    • Bulletin of the Korean Chemical Society
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    • v.9 no.4
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    • pp.202-206
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    • 1988
  • In order to investigate the oligomerization state of the plasma membrane proteolipid apoprotein purified from the bovine kidney, fluorescence polarization experiment was carried out in the two different solvent systems, i.e., water and organic solvent(chloroform-methanol). The molecular volumes of the proteins estimated from the Perrin equation, were to be 45,258$A^3$ and 17,608$A^3$ in water and organic solvent, respectively. These values indicate that a trimerization is possibly occurring in the aqueous environment. As an auxiliary experiment for the calculation of the molecular volume using Perrin equation, fluorescence quenching constants ($K_q$) with the quencher acrylamide and fluorescence lifetimes (${\tau}_F$) of the intrinsic fluorophore tryptophan residue were estimated in the two different solvent systems. $K_q$ in water was 18.21$M^{-1}$ and it was 46.24$M^{-1}$ in organic solvent. Fluorescence lifetimes of tryptophan residue were calculated to be 2.80 nsec. in water and 3.81 nsec. in organic solvent, respectively.

A Study on the Effect of Science Achievement Faculties Improvements in High School Inquiry Chemistry Experiments (탐구 화학 실험을 통한 고등학교 학생들의 과학 성취도 향상에 관한 연구)

  • Choonpyo Hong;Yongyeon Kim
    • Journal of the Korean Chemical Society
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    • v.47 no.5
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    • pp.487-494
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    • 2003
  • In this study, the effects of science achievement faculties improvements upon students' logical thinking, science inquiry skills, experiment attitudes, manipulated skills and inquiry process skills were investigated in high school inquiry chemistry experiments. For this purpose, the participants were 150 students taking the course of high school chemistry class in Anyang-city. Logical thinking and science inquiry skills' data were collected from written tests, inquiry process skills' data were collected from reports and experiment attitudes and manipulated skills' data were collected from classroom observations. As the results of this study, students' inquiry process skills, experiment attitudes and manipulative skills were very low. But other parts except inquiry process skills were significantly increased. And the science achievement of students were improved when continuously inquiry chemistry experiment were applied to students.

Effect of Tryptone and Tungsten in Medium on Syngas Fermentation Using Clostridium ljundahlii (Clostridium ljundahlii를 이용한 합성가스 발효에서 배지 내 tryptone 및 tungsten의 영향)

  • Soeun Park;Young-Kee Kim
    • Applied Chemistry for Engineering
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    • v.34 no.3
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    • pp.327-329
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    • 2023
  • In this study, an experiment was conducted to investigate the effect of the concentrations of tryptone, an organic nitrogen supplement, and sodium tungstate on the growth of microbial and the production of acetic acid and ethanol in the culture of Clostridium ljungdahlii. Microbial growth increased by 144.6%, and ethanol and acetic acid production improved by 8.6% and 36.7%, respectively, when 2.5 g/L of tryptone was added to the medium of the control experiment (0 g/L tryptone). In the experiment with 1 µM Na2WO4·2H2O, which is 100 times higher than the condition of the medium used in the control experiment (0.01 µM Na2WO4·2H2O), there was no significant difference in microbial growth or total production of C2 metabolites, but ethanol production increased and acetic acid production decreased. As a result, the ethanol/acetic acid production ratio increased significantly from 0.24 in the control experiment to 0.56.