• The Bulletin of The Korean Astronomical Society
• /
• v.40 no.1
• /
• pp.80.2-80.2
• /
• 2015

We present spatial and chemical distributions of Carbon-Enhanced Metal-Poor (CEMP) stars in the Milky Way's halo, as observed by the Baryon Oscillation Spectroscopic Survey (BOSS). Although the BOSS was designed to obtain spectra of galaxies and quasars, it also observed numerous metal-poor main-sequence turnoff stars for the purpose of flux calibration. The stars observed in the BOSS are two magnitudes fainter (15.5 < g < 19.2) than those in the legacy SDSS, thus it is an extremely useful sample to probe the distant halo. Using effective temperatures, surface gravities, [Fe/H], and [C/Fe] derived for these stars by the SEGUE Stellar Parameter Pipeline (SSPP), we investigate the spatial distribution of [Fe/H] and [C/Fe], the distribution of [C/Fe], and frequency of CEMP stars among these stars. These tools enable characterization of the origin of the halo and its initial mass function.

• Bulletin of the Korean Chemical Society
• /
• v.31 no.7
• /
• pp.2011-2014
• /
• 2010

The fluorescence intensity of a single-stranded oligonucleotide containing a fluorene-labeled deoxyuridine $(U^{Fl})$ unit increases by only 1.5-fold upon formation of its perfectly matched duplex. To increase the fluorescence signal during hybridization, we positioned a quencher strand containing a deoxyguanine (dG) nucleobase, functioning as an internal quencher, opposite to the $U^{Fl}$ unit to reduce the intrinsic fluorescence upon hybridization with a probe. From an investigation of the optimal length of the quencher strand and the effect of the neighboring base sequence, we found that a short strand (five-nucleotide) containing all natural nucleotides and dG as an internal quencher was effective at reducing the intrinsic fluorescence of a linear beacon; it also exhibited high total discrimination factors for the formation of perfectly matched and single base-mismatched duplexes. Such assays that function based on clear changes in fluorescence in response to single-base nucleotide mutations would be useful tools for accelerating diagnoses related to various diseases.

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.408-411
• /
• 2005

The green fluorescent protein (GFP) from the jellyfish aequorea and its fluorescent homologs from Anthozoa corals have become invaluable tools for imaging of cells and tissues. In this study various fluorescent protein such as green fluorescent protein (GFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP) have been expressed in Escherichia coli. Growth of recombinant cells and production of fluorescent proteins were investigated in shake flasks. Some characteristics of fluorescent proteins was also studied.

• Genomics & Informatics
• /
• v.8 no.4
• /
• pp.170-176
• /
• 2010

The Ribonucleotide reductases (RNR) are essential enzymes that catalyze the conversion of nucleotides to deoxynucleotides in DNA replication and repair in all living organisms. The RNRs operate by a free radical mechanism but differ in the composition of subunit, cofactor required and regulation by allostery. Based on these differences the RNRs are classified into three classesclass I, class II and class III which depend on oxygen, adenosylcobalamin and S-adenosylmethionine with an iron sulfur cluster respectively for radical generation. In this article thirty seven sequences belonging to each of the three classes of RNR were analyzed by using various tools of bioinformatics. Phylogenetic analysis, dot-plot comparisons and motif analysis was done to identify a number of differences in the three classes of RNRs. In this research article, we have attempted to decipher evolutionary relationship between the three classes of RNR by using bioinformatics approach.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.3 no.2
• /
• pp.3-9
• /
• 2004

The main objectives of this paper are to determine optimum cutting conditions using experimental design method to manufacture pedicle screws. Generally, titanium alloys are known as difficult-to cut materials. In the machining of titanium alloy, high cutting temperature and strong chemical affinity between the tool and the work material are generated because of Its low thermal conductivity and chemical reactivity. Such phenomenon cause increase of tool wear and deterioration of surface quality. Thus, in this paper, required experimental investigations are performed to evaluate the machinability of titanium materials With tungsten carbide tools Required simulation and experiments are performed, and the results are investigated.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2000.11a
• /
• pp.898-901
• /
• 2000

It is known that Si3N4 ceramic insert has less hardness than Al2O3 ceramic insert. But Si3N4 ceramic insert has not only high toughness and strength but also low thermal expansion coefficient, which makes it has longer tool life under thermal stress condition. In this study, commercial Si3N4 ceramic insert and home-made SiC-Si3N4 ceramic insert which has different sintering time and chemical composition is tested under various cutting conditions. The experimental result is compared in terms of tool life and cutting force. Generally, As the cutting speed and the feed rate increased, the cutting force and the flank wear increased too. The performance of SiC-Si3N4 ceramic insert shows the possibility to be a new ceramic tool.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2003.11a
• /
• pp.98-101
• /
• 2003

The Alkali-Aggregate Reaction (AAR) may cause a serious failure in the concrete structures. Several researchers in some nations have performed the continuous studies to prevent failure of a concrete structures by the AAR distress as well as the studies to manifest the mechanism. The ASTM Standards to prevent failure by potential AAR aggregates were established in 1950. The KS F2545 and KS F 2546 were established to test the susceptibility of aggregate to potential AAR in 1982. But the researches on the AAR have not been performed affluently in Korea because the distress due to AAR has seldom been reported officially. In this study, the Chemical Method and Scanning Electron Microscopy (SEM) were used to verifying the cause of the pattern crack on the surface and internal crack in the plain concrete pavement. It can be concluded that the distress of a specific site in plain concrete pavement was mainly due to AAR, and the chemical method and SEM may be the effective tools for verifying the cause of AAR distresses.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2000.11a
• /
• pp.31-35
• /
• 2000

We investigate the groundwater distribution and chemical characteristics for 3 or 5 districts every year and make the hydrogeologic map on a scale of 1:50,000. We draw the hydrogeologic digital map based on "The Handbook for the Drawing and Management of Hydrogeologic Map" which was published by MOCT and KOWACO in 1998. But, the Stiff diagram and well's notation are difficult to be presented in the digital map using the commercial Arcview GIS tools. So we develop the script file with Avenue language to represent them in Arcview GIS tool. At first, we design the database for the chemical analysis result of groundwater and well identification, and make the program code with Avenue language to display them on the digital map. And next we test the usefulness of the program code. As a result, we find that the script file is very useful for drawing the symbols and diagrams in hydrogeologic digital map using ArcView GIS.

• The Bulletin of The Korean Astronomical Society
• /
• v.36 no.2
• /
• pp.146.2-146.2
• /
• 2011

In this study, we have derived the abundances of several elements ? Na, Mg, Al, Si, Ca, Sc, Ti, V, Cr, Mn, Co, Ni - for the six F G K type stars in IC 2391 and the seven stars in NGC 6475. The spectra of those stars are taken from UVES POP archive data, of which resolution is 80,000. To derive the abundances of those elements, TAME (Tools for Automatic Measurement of Equivalent-widths), Kurucz stellar atmospheric model, and MOOG code are used. The stellar parameters (effective temperature, log g, metallicity, microturbulent velocity) are determined from the iron lines. The results provide the abundance differences of chemical elements between two open clusters, IC 2391 (a member of Gould Belt) and NGC 6475 (non-member of it), which would lead to better understanding about Gould Belt.

• Molecules and Cells
• /
• v.24 no.2
• /
• pp.268-275
• /
• 2007

Protein phosphorylation plays a key role in signal transduction in cells. Since phosphoproteins are present in low abundance, enrichment methods are required for their purification and analysis. Chemical derivatization strategies have been devised for enriching phosphoproteins and phosphopeptides. In this report, we employed a strategy that replaces the phosphate moieties on serine and threonine residues with a biotin-containing tag via a series of chemical reactions. Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO)-depleted protein extracts prepared from Arabidopsis seedlings were chemically modified for 'biotin-tagging'. The biotinylated (previously phosphorylated) proteins were then selectively isolated by avidin-biotin affinity chromatography, followed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This led to the identification of 31 protein spots, representing 18 different proteins, which are implicated in a variety of cellular processes. Despite its current technical limitations, with further improvements in tools and techniques this strategy may be developed into a useful approach.