• Natural Product Sciences
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• v.17 no.4
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• pp.296-302
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• 2011

We determined the inhibitory activity of the essential oil fraction obtained by steam distillation from the fresh and dried leaves of Perilla frutescens var. acuta against some pathogenic Salmonella and Vibrio spp. The activities of compounds isolated from the essential oils, apiol and myristicin, were also tested and the results were compared with those of the essential oil fraction. The Perilla essential oil fraction and its main components showed significant inhibition against antibiotic-susceptive and antibiotic-resistant strains of the tested Salmonella and Vibrio strains. Synergistic or additive effects were identified by combing the oils with ampicillin by checkerboard-titer tests. We conclude that essential oils from P. frutescens can be useful in the treatment of Salmonella and Vibrio infections and as safe additives to food materials for the prevention of contamination of food by these bacteria. This is especially important because of the rapid increase in antibiotic-resistant strains, which could cause severe symptoms in humans.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2004.10a
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• pp.411-418
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• 2004

Using a level set method we develop a shape optimization method applied to energy flow problems in steady state. The boundaries are implicitly represented by the level set function obtainable from the 'Hamilton-Jacobi type' equation with the 'Up-wind scheme.' The developed method defines a Lagrangian function for the constrained optimization. It minimizes a generalized compliance, satisfying the constraint of allowable volume through the variations of implicit boundary. During the optimization, the boundary velocity to integrate the Hamilton-Jacobi equation is obtained from the optimality condition for the Lagrangian function. Compared with the established topology optimization method, the developed one has no numerical instability such as checkerboard problems and easy representation of topological shape variations.

• Journal of Mechanical Science and Technology
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• v.18 no.2
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• pp.253-261
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• 2004

A simple and effective filtering method to control the member size of an optimized structure is proposed for topology optimization. In the present approach, the original objective sensitivities are replaced with their relative values evaluated within a filtering area. By adjusting the size of the filtering area, the member size of an optimized structure or the level of its topological complexity can be controlled even within a given finite element mesh. In contrast to the checkerboard-free filter, the present filter focuses on high-frequency components of the sensitivities. Since the present filtering method does not add a penalty term to the objective function nor require additional constraints, it is not only efficient but also simple to implement. Mean compliance minimization and eigenfrequency maximization problems are considered to verify the effectiveness of the present approach.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05b
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• pp.173-178
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• 1996

A three-dimensional thermo-hydraulic computer code is developed for simulation of incompressible flows in complex geometries. The computer code employs a body-fitted, nonorthogonal grid system in order to efficiently handle the complex geometries encountered in many engineering applications. The finite volume method is used to discretize the governing equations and the convection term is treated by higher-order bounded schemes. The cell-centered, nonstaggered grid arrangement is adopted and the resulting checkerboard pressure oscillation is avoided by use of momentum interpolation practice. The computer code employs the SIMPLE algorithm for pressure and velocity coupling and the k-$\varepsilon$ turbulence for turbulent calculation. The computer code has been tested through application to a variety of test problems and some results are presented in this paper

• Natural Product Sciences
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• v.13 no.1
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• pp.85-89
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• 2007

To determine whether the essential oil from Coriandum sativum and its main component, linalool, exhibit antifungal activity, we employed a broth dilution assay and disk diffusion test using common pathogenic Candida and Trichophyton species. Both coriander oil and linalool significantly inhibited growth of the tested fungi, with minimal inhibitory concentrations (MICs) in the range of $0.03{\sim}2mg/ml$. Furthermore, in a checkerboard titer test, both the oil fraction and linalool exhibited synergism when combined with ketoconazole, with resultant FICIs ranging from 0.06 to 0.53. Notably, hyphal formation in C. albicans cells was obviously inhibited by C. sativum essential oil in this experiment.

• Coupled systems mechanics
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• v.5 no.4
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• pp.329-340
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• 2016

The trigonal shape memory alloys (SMAs) have a great potential to be utilized as the applications with special purposes, such as actuators with high operation frequency. Most studies on the trigonal microstructures typically focus on the well-known classic herringbone pattern, but many other patterns are also possible, such as non-classic herringbone, toothbrush and checkerboard patterns. In the current work, a systematic procedure is developed to find all possible laminate twin microstructures by using geometrically linear compatibility theory. The procedure is verified by SEM images with the information of crystallographic axes of unitcells obtained by EBSD, showing good agreement. Many interesting trigonal R-phase patterns are found in the specimen. Then, their incompatibility are analyzed with nonlinear compatibility theory. The relationship between such incompatibility and the likelihood of occurrence of the microstructures is revealed. The current procedure is rapid, computationally efficient and sufficiently general to allow further extension to other crystal systems and materials.

• Journal of computational fluids engineering
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• v.4 no.2
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• pp.39-46
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• 1999

The FVM(Finite Volume Method) have been used mainly for the flow analyses in the piston-cylinder. The objective of the present study is to analyze numerically the piston-driven intake flows using the FEM(Finite Element Method). The FEM algorithm used in this study is 4-step time-splitting method which requires much less execution time and computer storage than the velocity-pressure integrated method and the penalty method. And the explicit Lax-Wendroff scheme is applied to nonlinear convective term in the momentum equations to prevent checkerboard pressure oscillations. Also, the ALE(arbitrary Lagrangian Eulerian) method is adopted for the moving grids. The calculated results show good agreement in comparison with those by the FVM and the experimental results by the LDA.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.8
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• pp.786-792
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• 2009

Topology optimization is to find the optimal material distribution of the specified design domain minimizing the objective function while satisfying the design constraints. Since the genetic algorithm (GA) has its advantage of locating global optimum with high probability, it has been applied to the topology optimization. To guarantee the structural connectivity, the concept of compliance pattern is proposed and to improve the convergence rate, small number of population size and variable probability in genetic operators are incorporated into GA. The rank sum weight method is applied to formulate the fitness function consisting of compliance, volume, connectivity and checkerboard pattern. To substantiate the proposed method design examples in the previous works are compared with respect to the number of function evaluation and objective function value. The comparative study shows that the compliance pattern based GA results in the reduction of computational cost to obtain the reasonable structural topology.

• Natural Product Sciences
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• v.11 no.2
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• pp.92-96
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• 2005

The antifungal activities of essential oils from the leaves of Glehnia littoralis, which is cultivated in Korea, were evaluated against pathogenic Trichophyton species by the broth dilution method and the disk diffusion test. Additionally, the effects of the oils together with ketoconazole were tested by the checkerboard titer test. The essential oil fraction and its main components showed significant inhibition of the tested Trichophyton fungi, with minimal inhibitor concentrations (MICs) in the range of 16-32 mg/ml. The results suggest that activities of this oil are based mainly on the contents of ${\alpha}-pinene$ (22.17%), the next prominent component of the oil fraction, while the first main components ${\beta}-pinene$ (57.83%) have relatively mild activity. The MICs of ${\alpha}-pinene$ and ${\beta}-pinene$ were 1-4 mg/ml and 4-32 mg/ml, respectively. Additionally the Glehnia oil fraction and its main components as well, exhibited significant synergism with ketoconazole against Trichophyton rubrum.

• Natural Product Sciences
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• v.16 no.3
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• pp.175-179
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• 2010

The present study aimed to assess the antifungal properties of the essential oil fraction from Ligusticum chuanxiong (Umbelliferae) and its components against five clinically important Aspergillus species. The essential oil fraction was extracted from the underground parts of the plant by steam distillation, and its main components, namely, Z-ligustilide, butylidene phthalide, and p-cresol were isolated by column chromatography. The antifungal activities of the essential oils were evaluated by the broth dilution method. Both the total essential oil fraction of L. chuanxiong and its components showed significant anti-Aspergillus activity against all five tested strains with MICs between 62.5 and 250 ${\mu}g$/ml, respectively. In a checkerboard microtiter assay, the combination of antibiotics, itraconazole with the essential oil fraction of L. chuanxiong or its main components exhibited synergistic or additive, and in some cases indifferent, effects against the tested Aspergillus species, resulting in FICIs (fractional inhibiting concentration indices) ranging from 0.12 to 2, while the combination of antibiotics, amphothericin B with L. chuanxiong essential oils mostly showed antagonistic effects.