• Development and Reproduction
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• v.19 no.1
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• pp.25-31
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• 2015

We have launched an investigation for Embryonic Development, Larvae and Juvenile Morphology, of Buenos aires tetra in order to build basic data of Characidae and fish seeding production. We brought 50 couples of Characidae from Bizidduck aquarium in Yeosu-si, Jeollanamdo, from Korea on March of 2015. We put them in the tetragonal glass aquarium ($50{\times}50{\times}30cm$). Breeding water temperature was $27.5{\sim}28.5^{\circ}C$ (mean $28.0{\pm}0.05^{\circ}C$) and being maintained. The shape of fertilized egg was round shape, and it was adhesive demersal egg. The egg size was 0.63~0.91 mm (mean $0.74{\pm}0.07mm$, n=20). After getting fertilized egg, the developmental stage was gastrula stage, and embryo covered almost two-thirds of Yolk. Incubation was happened after 16 hours 13 minutes from gastrula stage, and the tail of juvenile came out first with tearing egg capsule. Immediately after the incubation, prelarvae had 3.78~3.88 mm length (mean $3.84{\pm}0.04mm$, n=5), and it had no mouth and anus yet. 34 days after hatching from the incubation, juvenile had 8.63~13.1 mm (mean $10.9{\pm}1.66mm$), and it had similar silver-colored body shape with its mother.

• Applied Microscopy
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• v.35 no.2
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• pp.89-96
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• 2005

The ultrastructures of the fertilized egg envelope from Hyphessobrycon serpae belonging to Characidae was studied using scanning and transmission electron microscopes to get systematic fundamental data for classification of species and to confirm whether micropyle is a common trait of Characidae or not. The fertilized egg was of colorless, transparent, spherical, adhesive and demersal type. There were not oil droplets in vitelline membrane and attached structures in the outside of fertilize egg envelope. The egg envelope had a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by 13 to 15 protruded lines of the egg envelope in a radiated form. The outer surface of fertilized egg envelope was covered by reticular adhesive fibrous structures and irregularly arranged by pore canals. The fertilized egg envelope consisted of three distinct layers an outer adhesive fibrous layer with high electron density, a middle layer with pore canals, and an inner layer consisting of 6 to 7 lamellae alternating layers with interlamellae of lower electron density. These ultrastructural characters of fertilized egg envelope form Hyphessobrycon serpae can be utilized in taxonomy of teleost, and as fundamental data for study on early development of fertilized egg. It seems that the morphology of micropyle is a common trait of Characidae

• Applied Microscopy
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• v.26 no.3
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• pp.277-291
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• 1996

The structures of the egg envelope in fertilized eggs of three species of characidae, head and tail light fish (Hemigrammus ocellifer), black tetra (Gymnocorymbus ternetzi), and buenos aires tetra (Hemigrammus caudovittatus) were studied using light and electron microscopes. The fertilized eggs in all species were colorless, transparent, spherical and non-floted type. The egg envelopes have a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by protruded lines of the egg envelope in a radiated form. Egg envelopes of fertilized eggs in both head and tail light fish and buenos aires tetra consisted of three distinct layers; an outer layer, a middle layer and an inner layer. And that of blacktetra consisted of two layers; an outer layer and an inner layer. Also, an outer layers of both head and tail light fish and black tetra were adhesive types but, in that of buenous aires tetra was non-adhesive type. An outer surface of egg envelope in black tetra was arranged by pores regularly. In that of head and tail light fish and buenos aires tetra have a rough side. An inner layer of egg envelope in fertilized eggs consisted of lamellae alternating with interlamellae of lower electron density; an inner layer of fertilized eggs in head and tail light fish consisted of three layers, that of black tetra was four layers, and that of buenos aires tetra was five layers.

• Applied Microscopy
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• v.38 no.4
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• pp.315-319
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• 2008

Glow-light tetra, Hemigrammus erythrozonus is a teleost belonging to Characidae. The natural habitate of this fish is the wild in the Essequibo river, Guyana and South America. The oogenesis of glow-light tetra was investigated by light microscopy to compare with those of other families. A pair of ovary was located between swim bladder and intestines. The ovary was of white color and ellipsoidal shape with the major axis 11 mm and the minor axis 4 mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and size of egg were increased, basophilic substance was distributed in only around the egg envelope. The yolk vesicles were changed to yolk mass in accordance with development. In conclusion, the oogenesis of glow-light tetra, Hemigrammus erythrozonus was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilia a in the cytoplasm. The oogenesis of glow-light tetra seems to share common patterns in Characidae, teleost and have a similar pattern with other teleost.

• 한국전자현미경학회:학술대회논문집
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• 2005.11a
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• pp.125-126
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• 2005

• Development and Reproduction
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• v.18 no.4
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• pp.241-249
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• 2014

Hyphessobrycon eques is a famous fish for ornamental fish market and aquarium. They are inhabit in regions of Amazon and Paraguay River basin. Serpae fishs were investigated 2-3 males are chased to female, and then males attempted to simulate the females abdomen. After fertilization, eggs were kept in incubators at $28^{\circ}C$. The fertilized eggs had adhesive and demesal characteristics and had a mean diameter of $0.92{\pm}0.01mm$. Larvae hatched at 16 hrs post fertilization. The hatched larvae averaged $2.90{\pm}0.16mm$ in total length ($L_T$). Complete yolk sac resorption and mouth opening occurred on the third day post hatching. At 45 days post hatching, the larvae were $12.5{\pm}1.60mm$ $L_T$ and had reached the juvenile stage.