• Journal of Pharmaceutical Investigation
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• v.26 no.1
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• pp.33-41
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• 1996

In order to study the effect of phenobarbital(PB) on the hepatic transport of diltiazem(DTZ), $Ca^{2+}$ channel blocker, we used isolated hepatocytes of rat which was intraperitoneally pretreated with phenobarbital sodium(75 mg/kg) for four days once a day. For the isolation of rat liver cells, a modification of the two step procedure of Seglen was used. DTZ was dissolved in incubation buffer to the final DTZ concentrations of 200, 400, 600, 800 and 1000 ng/ml in order to elucidate the uptake characteristics of DTZ by hepatocytes. Reactions were stopped at 10, 20, 30, 45, 60, 90, 120 and 300 sec. The initial velocity was determined by disappearance of diltiazem in the hepatocyte suspension. On the other hand, to determine the effect of PB on the in vitro hepatic intrinsic clearance of DTZ we obtained the metabolism rates of DTZ in the control and the PB-pretreated rat hepatocyte at various time intervals. According to pretreatment with PB, the size of hepatocyte and the amount of protein per $10^6$ cells were significantly (p<0.01) increased from $26.92{\pm}0.1364\;m$ to $35.31{\pm}1.00\;m$ and from $468{\pm}6.5\;{\mu}g/10^6$ cells to $628.8{\pm}12.1{\mu}g/10^6$ cells, respectively. In the case or hepatic uptake of diltiazem, $K_m$ was not different in the normalization by cell numbers and increased from $2.90\;{\mu}M\;to\;13.89\;{\mu}M$ in the normalization by protein amount. $V_max$ was increased regardless of normalization by protein amount and cell numbers, from $1.21\;{\mu}mole/min \;{\cdot}\;mg\;protein\;to\;3.96\;{\mu}mole/min\;{\cdot}\;mg\;protein\;and\;from\;2.38\;{\mu}mole/min\;{\cdot}\;10^6\;cells\;to\;2.83\;{\mu}mole/min\;{\cdot}\;10^6\;cells$, respectively. The in vitro hepatic intrinsic clearance of DTZ was significantly (p<0.01) increased from $0.640{\pm}0.038\;ml/mim\;{\cdot}\;10^6\;cells\;to\;2.385{\pm}0.212\;ml/min\;{\cdot}\;10^6\;cells$ due to PB-pretreatment. These results suggest that the uptake of DTZ by hepatocyte is extremely fast and PB enhances the hepatic intrinsic metabolic clearance of DTZ.

• Geophysics and Geophysical Exploration
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• v.12 no.4
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• pp.316-327
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• 2009

A standard for national seismological observatory was proposed on 1999. Since then, Quanterra digitizer has been installed and is operating on almost all of seismic stations which belong to major seismic monitoring organizations. Quanterra digitizer produce and transmit real-time event packet and data packet. Characteristics of event packet and arrival time of each channel's data packet on data center were investigated. Packet selection criteria using signal to noise ratio (hereafter SNR) and signal period from real-time event packet based on 100 samples per second (hereafter sps) velocity data were developed. Estimation of epicenter using time information of the selected event packet were performed and tested. A series of experiment show that event packets were received approximately 3~4 second earlier than data packets and the number of event packet was only 0.3% compare to data packets. Just about 5% against all of event packets were selected as event packet were related P wave of real earthquake. Using the selected event packets we can estimate an epicenter with misfit less than 10 km within 20 sec for local earthquake over magnitude 2.5.

• Journal of the Society of Naval Architects of Korea
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• v.33 no.1
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• pp.27-39
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• 1996

The effects of free surface and a strut on the lifting characteristics on a fin attached at the mid-chord of the strut are investigated experimentally in a circulating water channel. Variation of lift force on the fin is investigated with respect to free stream velocity(V), angle of attack of the fin(${\alpha}$) and ratio of the submergence depth of the fin to the chord of the fin(H/C). Attentions are focused on the lifting characteristics of the fin at shallow depths of submergence. Visualization of the free surface deformation along the strut and of the streamline about the fin is made in order to examine the inflow angle to the fin. Lift force on the fin alone i.e. in absence of the strut is also measured to investigate the difference in lifting characteristics of the fin caused by the strut. The results show that lift forces over the fin are largely affected by a free surface in the case of small submergence ratios(HiC<3.0). For HiC>5.0, the effects of the free surface are negligibly small. The inflow angle to the fin is significantly influenced by the strut and flow speed at the shallow depths of submergence. The deformation of the free surface is largely governed by the waves generated by the strut. However, for small submergence depths, the effects of the fin are found also significant.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.2
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• pp.495-506
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• 2013

This study developed the Time-split Mixing Model (TMM) which can represent the pollutant mixing process on a three-dimensional open channel through constructing the conceptual model based on Taylor's assumption (1954) that the shear flow dispersion is the result of combination of shear advection and diffusion by turbulence. The developed model splits the 2-D mixing process into longitudinal mixing and transverse mixing, and it represents the 2-D advection-dispersion by the repetitive calculation of concentration separation by the vertical non-uniformity of flow velocity and then vertical mixing by turbulent diffusion sequentially. The simulation results indicated that the proposed model explains the effect of concentration overlapping by boundary walls, and the simulated concentration was in good agreement with the analytical solution of the 2-D advection-dispersion equation in Taylor period (Chatwin, 1970). The proposed model could explain the correlation between hydraulic factors and the dispersion coefficient to provide the physical insight about the dispersion behavior. The longitudinal dispersion coefficient calculated by the TMM varied with the mixing time unlike the constant value suggested by Elder (1959), whereas the transverse dispersion coefficient was similar with the coefficient evaluated by experiments of Sayre and Chang (1968), Fischer et al. (1979).

• Geophysics and Geophysical Exploration
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• v.14 no.3
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• pp.227-233
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• 2011

Attention has been focused on geothermal energy as an alternative energy because it is continuously operable without external supply. Most of geothermal anomalies in Korea are related to deep circulation of groundwater through a fracture system in granite area. Therefore it is very important to understand the distribution of the fracture system which is the main channel of ground water. In this research, we constructed the velocity models with a fracture system and the layered sediments, respectively, and generated synthetic data sets with them to verify the presented vertical seismic profiling (VSP) preprocessing scheme. We compared the results from conventional VSP preprocessing flow to those from VSP preprocessing flow considering fracture system. We noticed that the preprocessing flow considering fracture system retains more sufficient signal including down-going wave than conventional preprocessing. In addition, we applied 3D VSP prestack phase screen migration to the preprocessed reversed VSP (RVSP) data from Seokmo Island so that we were able to image fracture structure of the geothermal site in Seokmo Island.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.29 no.2
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• pp.92-108
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• 2017

Present work shows a numerical method to analysis of interaction analysis between solitary wave and onshore bridge. Numerical simulation is carried out by TWOPM-3D (three-dimensional one-field model for immiscible two-phase flows), which is based on Navier-Stokes solver. To do this, the solitary wave is generated numerically in numerical wave channel, and numerical results and experimental results were compared and analyzed in order to verify the applicability of force acting on an onshore bridge. From this, we discussed precisely the characteristics of horizontal and vertical forces (uplift and downward forces) changes including water level and velocity changes due to the variation of solitary wave height, water depth, onshore bridge's location and type, and number of girder. Furthermore, It is revealed that the maximum horizontal and vertical forces acting on the girder bridge show different varying properties according to the number of girder, although each maximum force acting on the girder bridge is proportional to the increasement of incident solitary wave height, and the entrained air in the fluid flow affects the vertical force highly.

• Journal of Biomedical Engineering Research
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• v.21 no.4
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• pp.391-401
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• 2000

Flow through compliant tubes with linear taper in wall thickness is numerically simulated by finite element analysis. For verification of the numerical method, flow through a compliant stenotic vessel is simulated and the results are compared to the existing experimental data. Steady two-dimensional flow in a collapsible channel with initial tension is also simulated and the results are compared with numerical solutions from the literature. Computational results show that as cross-sectional area decreases with the reduction in downstream pressure, flow rate increases and reaches the maximum when the speed index (mean velocity divided by wave speed) is near the unity at the point of minimum cross-section area, indicating the flow limitation or choking (flow speed equals wave speed) in one-dimensional studies. for further reductions in downstream pressure, flow rate decreases. The flow limitation or choking consist of the main reasons of waterfall effect which occurs in the airways, capillaries of lung, and other veins. Cross-sectional narrowing is significant but localized. When the ratio of downstream-to-upstream wall thickness is 2, the area throat is located near the downstream end. As this ratio is increased to 3, the constriction moves to the upstream end of the tube.

• Journal of Korea Water Resources Association
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• v.50 no.8
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• pp.521-535
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• 2017

Acoustic Doppler Current Profilers (ADCPs) have been widely utilized for assessing streamflow discharge, yet few comprehensive studies were conducted to evaluate discharge uncertainty in consideration of individual uncertainty components. It could be mostly because it was not easy to determine which uncertainty framework can be appropriate to rigorously analyze streamflow discharge driven by ADCPs. In this regard, considerable efforts have been made by scientific and engineering societies to develop a standardized theoretical framework for uncertainty analysis in hydrometry. One of the well-established UA methodology based on sound statistical and engineering concepts is Guide to the Expression of Uncertainty Measurement (GUM) adopted widely by various scientific and research communities. This research fundamentally adapted the GUM framework to assess individual uncertainty components of ADCP discharge measurements, and subsequently provided results of a customized experiment in a controllable real-scale artificial river channel. We focused particularly upon sensitivities of uncertainty components in the GUM framework driven by ADCPs direct measurements such as depths, edge distance, submerged depth, velocity gap, sampling time, repeatability, bed roughness and so on. Section-by-Section method for ADCP discharge measurement was applied for uncertainty analysis for this study. All of measurements were carefully compared with data using other instrumentations such as ADV to evaluate individual uncertainty components.

• Journal of Korea Water Resources Association
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• v.53 no.8
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• pp.617-626
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• 2020

This study investigates changes of bifurcation discharge ratio, flow velocity distributions and characteristics of separation zone due to variation of bifurcation angle by using TELEMAC-2D model. When the bifurcation angle is reduced from 90° to 45° without changing the boundary conditions, the bifurcation discharge ratio increased by 1.5 times from 0.523 to 0.785 because of increasing the radius of curvatures, the inertia force of the downstream flow, and the pressure gradient by the downstream boundary conditions. The bifurcation discharge ratio increases non-linearly whenever the bifurcation angle decreases by 15° intervals from 90° to 45° in flow with the upstream Froude number of 0.45 to 0.74. In flow with a maximum Froude number of 0.74, the rate of increase for bifurcation discharge ratio is 31.1% and the minimum value. When the Froude number is 0.58, the bifurcation discharge ratio is 0.7 or less, and the maximum rate of increase for that ratio is 53.5%. As the upstream Froude number decreases less than 0.58, the bifurcation discharge ratio exceeds 0.7, and the rate of increase decreases. When the upstream Froude number is 0.4 higher, the dimensionless width and length changing ratio of the separation zone are about 2.56 and 5.5 times higher than in 0.4 or less.

• Tunnel and Underground Space
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• v.20 no.2
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• pp.82-91
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• 2010

Grouting technology is one of the ground improvement methods used in water controlling and reinforcement of rock mass in underground structure construction. It is necessarily required to find out the characteristics of grout flow through discontinuities in a rock mass for an adequate grout design and performance assessment. Laminar flow is not always applicable in simulating a grout flow in a rock mass, since the rock joints usually have apertures at a micro-scale and the flow through these joints is affected by the joint roughness and the velocity profile of the flow changes partially near the roughness. Thus, the influence of joint roughness and aperture on the grout flow in rough rock joint was numerically investigated in this study. The commercial computational fluid dynamics code, FLUENT, was applied for this purpose. The computed results by embedded Herschel-Bulkley model and VOF (volume of fluid) model, which are applicable to simulate grout flow in a narrow rock joint that is filled with air and water, were well compared with that of analytical results and previously published laboratory test for the verification. The injection pressure required to keep constant injection rate of grout was calculated in a variety of Joint Roughness Coefficient (JRC) and aperture conditions, and the effect of joint roughness and aperture on grout flow were quantified.