• Journal of the Korean Wood Science and Technology
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• v.26 no.4
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• pp.20-25
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• 1998

Radial variation of crystalline state in a stem of Chamaecyparis obtusa E. was examined by x-ray diffraction analyses. Relative crystallinity and degree of crystallite orientation showed significant differences between juvenile and adult wood. That is, Relative crystallinity increased with increasing the age from pith to about 20th annual ring, after which it reached a more or less constant value. On the other hand, degree of crystallite orientation decreased outward from pith to about 10 years and presented almost a constant value thereafter. Crystal width by Scherrer's equation did not show any significant differences between juvenile and adult wood. Therefore, it was considered that crystallinity index and degree of crystallite orientation by x-ray diffraction method could be used for evaluating wood quality.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.29 no.1
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• pp.33-46
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• 2016

Objective : The aim of this study is to investigate the effect of Chamaecyparis Obtusa Oil to NC/Nga mice induced in Atopic dermatitis-like skin lesions by DNCB Mthods.Methods : NC/Nga mice which have been induced to Atopic dermatitis-like skin lesions by DNCB are divided into 3 groups, the one is the mice which have been spread with Chamaecyparis Obtusa Oil 2% cream, the other is which have been spread with dexamethasone (Dexa.) 0.5% on their Atopic lesion, another is the control group. then we take the blood sample of each group to measure state of the dorsal skin, the number of immunocytes. and resect the skin lesion to analyze the state of cells.Result & Conclusion : There are meaningful results of measuring the number of IgE, IL-4, IFN-γ and the state of the skin lesion and cells in the group with hamaecyparis Obtusa Oil 2% cream in comparison with the other groups.

• Journal of Korean Society of Forest Science
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• v.96 no.5
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• pp.528-532
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• 2007

Clonal differences in fertility (expressed as the number of female and male strobili) were determined for five consecutive years (2002-2006) in a clonal seed orchard of Chamaecyparis obtusa. Fertility varied among clones and among years with producing five-year averages of 378.8 and 871.2 for female and male strobili per ramet, respectively. Correlation between female and male strobilus production was positive over the five years and statistically significant. Based on the observed fertility variation, the effective population sizes (estimated by status numbers, $N_e$) were calculated and varied from 24.3 to 47.9 (48.6% to 95.8% of census number, N) among the five studied years. On average (pooled), the relative effective population size was 82% of the N. Variation in female fertility was higher than that in male fertility, and this variation was reflected on female and male parents' status numbers. Pooled $N_e$ estimated from the five years was higher than that from poor seed production years. From our results, it was concluded that genetic diversity collected from good flowering years would be higher than that from poor flowering years.

• The Korea Journal of Herbology
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• v.32 no.1
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• pp.75-81
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• 2017

Objectives : The present study was designed to investigate effects of mixed extracts (9:1, v/v) of Hwangryunhaedoktang, Chamaecyparis obtusa essential oil. We evaluated the antimicrobial and antioxidant activity by manufacturing mixed extracts as the materials for functional medicinal herb cosmetics. Methods : We performed antimicrobial were tested microbes (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, Propionibacterium acnes) by disc diffusion method and measure clear zone. Antioxidant activities were measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. Results : Antimicrobial activities of mixed extracts against S. epidermideis, P. acnes, S. aureus, E. coli, and Candida. A were $10.9{\pm}3.0mm$, $16.9{\pm}2.0mm$, $9.2{\pm}2.0mm$, $10.3{\pm}1.0mm$, $11.8{\pm}3.0mm$ respectively. The Chamaecyparis obtusa essential oil had the highest antimicrobial activities against S. epidermideis, P. acnes, S. aureus, E. coli, and Candia. A and clear zone of microbes ware $16.7{\pm}3.0mm$, $28.8{\pm}0.2mm$, $15.9{\pm}2.0mm$, $11.5{\pm}1.0mm$, $16.3{\pm}3.0mm$. Hwangryunhaedoktang extract showed antimicrobial activity but only P. acnes and S. aureus The antioxidant activities of the mixed extracts were tested through the evaluation of DPPH radical scavenging activity. The 100 % mixed extracts were found to have 90 % DPPH radical scavenging activity. The mixed extracts was presented similar antioxidant activities compared with that of ascorbic acid. As a result, A mixture extract is expected to have antimicrobial effects and free radical scavenging activity was found. Conclusions : Accordingly, It can be concluded that mixed extracts has the potential to cosmetic material.

• Journal of Life Science
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• v.29 no.7
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• pp.785-791
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• 2019

In this study, we investigated the biological antioxidant and antibacterial activity of Chamaecyparis obtusa (C. obtuse) extracts by measuring DPPH radical scavenging and ABTS radical scavenging, and SOD-like activities. The DPPH and ABTS radical scavenging activities were increased in a dose-dependent manner, with maximum activities of 78% and 62% at an extract concentration of $50{\mu}l/ml$. The C. obtusa extracts also showed high SOD-like activity, with a maximum activity of 92.85% at a concentration of $50{\mu}l/ml$. The antibacterial activities of C. obtusa extracts were measured against six types of bacteria known to cause food poisoning and disease. Antibacterial activity was investigated against three gram-positive and three gram-negative bacteria using the paper disc agar diffusion method. The C. obtusa extracts showed antibacterial activities against B. cereus, E. coli, L. monocytogenes, S. aureus, S. typhi and V. parahaemolyticus, among which the activity against B. cereus was greatest. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of C. obtusa extracts were $30-40{\mu}l/ml$ for the 6 strains that showed an antimicrobial response by the paper disc agar diffusion method. These results suggest that C. obtusa extracts could serve as potential antibacterial agents to inhibit the growth of pathogens responsible for food poisoning and disease.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.237-244
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• 2015

In this study, essential oils were extracted from the leaf of Chamaecyparis obtusa (CLEO), indigenous to Korea, CLEO constituents were analysed, and the effects of CLEO on airway hyperresponsiveness (AHR) and airway inflammation (AI) were investigated in Ovalbumin (OVA)-induced asthma mouse model. Terpenoid components among identified CLEO constituents made up more than 80%. The CLEO-treated group in comparison to the control group showed reduced AHR, the decrease of eosinophil number in the bronchoalveolar lavage fluid (BALF), reduced specific anti-OVA IgE level in the serum, and a significant reduction in Th2 cytokines levels in the BALF with concentration. We concluded that CLEO have an alleviating effect on asthma-like symptoms such as AHR and AI. Further studies about antiasthmatic effect are necessary on the focus of single component of CLEO.

• Journal of the Korean Wood Science and Technology
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• v.36 no.6
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• pp.159-167
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• 2008

This study was carried out to investigate the potential promise of Chamaecyparis obtusa oil as a natural antioxidant. C. obtusa oil and its fractions were subjected to screening for their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and ammonium thiocyanate method. In the first case, $IC_{50}$ value of the C. obtusa oil was determined as $40{\mu}{\ell}/m{\ell}$. At $0.5{\mu}{\ell}/m{\ell}$ concentration level, free radical scavenging effect of fraction G determined as 66.94% was the highest among the fractions of C. obtusa oil. In the ammonium thiocyanate method, essential oil of C. obtusa and its fraction C, D, and E showed activities of 72.0%, 71.2%, 71.9% and 71.1%, respectively. Fraction G, most active fraction, was mainly consisted of $\alpha$-terpineol, elemol, widdrol and $\alpha$-cadinol.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.4 s.48
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• pp.503-507
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• 2004

The essential oil from Chamaecyparis obtusa was investigated for biological activities in anti-oxidative, anti-inflammation and antibacterial method, respectively. The Growth inhibitory effect of C. obtusa oil on the bacteria was evaluated with MIC (minimum inhibitory concentration), $IC_{50}\;(50\%$ inhibitory concentration), and paper disc method. Two kinds of gram positive strains and two kinds of gram negative strains were used in this study. Gram positive strains were B. subtilis and S. aureus. and Gram negative strains were E. coli and P. aeruginosa. Gram positive strains showed much more intensive effect than gram negative strains. Anti-oxidative effect was investigated with DPPH (1,1-diphenyl-2-picrylhidrazyl) in methanol based and $IC_{50}\;was\;0.78\%.$ Our results suggest that the essential oil from Chamaecyparis obtusa has effects on anti-bacterial, anti- oxidative and anti-inflammation in in vitro and in uiuo. Then this material could be expect synergic effect with other candidated extracts and oils.

• Journal of Aquaculture
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• v.14 no.2
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• pp.81-87
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• 2001

Antimicrobial activity of the essential oil extracted from plants Artemisia princeps var. orientalis, Thuja orientalis and Chamaecyparis obtusa were tested against selected pathogenic bacteria and fungi of fish. At the concentrations above 500ppm, ingibitory effect of the oil of A. princeps var. orientalis was at its peak against Aeromonas hydrophila ATCC 14715, A. hydrophila CF-2, A. salmonicida ATCC 14174 and A. salmonicida EL-1 but the bacteria Edwardsiella tarda KBF-1, Vibrio anguillarum ATCC19264, V. ordalii ATCC33509 and Streptococcus sp. were insensitive. Likewise, the oil extract of T. orientalis showed the highest inhibitory activity against V. ordalii ATCC33509, E. tarda ECK-1, and E. tarda KBF-1 at 300ppm; however the activity was highest at 500ppm or A. hydrophila ATCC14715, A. hydrophila CF-2, A. salomonicida ATCC14174, A. salmonicida EL-1 and Streptococcus sp. SF-1. With increasing dose of C. obtusa oil, the inhibitory activity became more and more effective against A. hydrophila CF-2, A. salomonicida ATCC14174, E. tarda ECK-1 and Streptococcus sp. SF-1, but A. hydrophila ATCC14174, A. salmonicida EL-1, E. tarda KBF-1, V. anguillarum ATCC19264, V. ardalii ATCC33509 and gram positive bacteria (Streptococcus sp.) were somewhat resistant. A. princeps var. orientalis, T. orientalis and C. obtusa were also tested against Saprolegnia sp. at the oil concentrations of 10, 100, 500, 1,000, 1,500 and 2,000ppm. The inhibitory effect of the oil on the inhibit the mycelial growth of Saprolegnia sp. at 10ppm and completely inhibited at over 500ppm.

• Journal of Korean Society of Forest Science
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• v.103 no.3
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• pp.375-382
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• 2014

The objective of this study was to investigate suitable plantation site for planted Chamaecyparis obtusa seedlings from the analysis of regional early growth performances. Two years old C. obtusa seedlings were planted with the density of $3,900seedlings{\cdot}ha^{-1}$ in late March, 2011 at Haeman and Jangseong, Jeollanamdo. In each study site, three plots ($400m^2$ per plot) were established and root collar diameter (mm) and tree height (cm) of each C. obtusa were measured in April, 2011 and October from 2011 to 2013. We also analyzed soil physical and chemical properties of sites and compartmental nitrogen and phosphorus concentrations of C. obtusa. Although the concentrations of soil nitrogen, organic matter, and C.E.C. at Haenam site were higher than those at Jangseong site, early growth performances of planted C. obtusa at Jangseong site were significantly better than those at Haenam site. The reasons for these results were probably related to deep available soil depth at Jangseong site and relatively low annual precipitation and sea wind at Haenam site, which was adjacent to the sea. The compartmental nitrogen and phosphorus concentrations of C. obtusa was in the order of needles > current twigs > fine root > stem and branches root above 2 mm in diameter. The phosphorus concentration of needles at Haenam site was significantly higher than that at Jangseong site. The results of this study might be useful for the selection of suitable plantation site for C. obtusa.